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The map label for this gene is hmeA [H]

Identifier: 221632494

GI number: 221632494

Start: 464509

End: 465234

Strand: Reverse

Name: hmeA [H]

Synonym: trd_0468

Alternate gene names: 221632494

Gene position: 465234-464509 (Counterclockwise)

Preceding gene: 221632495

Following gene: 221632493

Centisome position: 23.23

GC content: 57.44

Gene sequence:

>726_bases
ATGCCACGCTGGGGAATGGTGATCGATCTTGACCGCTGTATCGGGTGCGGCGCCTGCGTCGTCGCGTGCCAGGCAGAGAA
CAACGTTCCGATCAATACCGAGGACGTCTATCATCAGCGCCGCGCCATCGCCTGGATTCGACTCGAGCGGTACTGGGAGG
GCGAATACCCGAACATCAAGGCACGCTACATTCCGGTACCCTGCATGCAGTGCACCAACGCTCCTTGCGAACCGGTCTGT
CCGGTTTATGCGACCTACCACACCAACGAAGGTATCAATATGCAGGTTTATAACCGCTGTATCGGCACTCGCTATTGTGC
GGTCAACTGCCACTGGCACGTCCGCTTCTTCAACTTCTGGGAGCCGAAGTGGCCCGATTCGCTCAAGAACCAGCTCAATC
CAGACGTCACGGTACGGAGTCGTGGCGTCATGGAAAAGTGCAACTTCTGTGTCCATCGCCTCGTCAAGGCCGAGCAGAAG
GCGAAGACTGAAAAGCGATCCCTGCGTACTGACGAGGTCACGACTGCTTGTGCCCAGGCATGTCCGACCAGTGCCATCGT
CTTCGGAGATCTGGACGACCCCAACAGCGCGGTCAGCAAGTTGCTCGCCCAGGCTGAACAGGAAGGACGCGTCTACCGGT
TGTTGGAAGATCTCGGTACCGATCCGAAGGTCTATTACCTGAAGAAGATCGATCTCGAAGCTGCTGAGCACGCAGCAACG
CACTGA

Upstream 100 bases:

>100_bases
TATGGTGCCAGCGTTCCAGCTGGAGGAGTTCCCGATCGTCCGCGTGATGGCACCGAAGGCCTGAGCGGGAGGGATCAGGG
AGAGGAAAAGGACGGGACGC

Downstream 100 bases:

>100_bases
GCGCTGATGAGCGAACGGGGTGAGGGGATATGGCGACACACGGTCACGCACACGGTCATGAACTCCAACGCACCTGGGAA
CTCGCACCGGCGGACGTGCT

Product: oxidoreductase, iron-sulfur cluster-binding subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MPRWGMVIDLDRCIGCGACVVACQAENNVPINTEDVYHQRRAIAWIRLERYWEGEYPNIKARYIPVPCMQCTNAPCEPVC
PVYATYHTNEGINMQVYNRCIGTRYCAVNCHWHVRFFNFWEPKWPDSLKNQLNPDVTVRSRGVMEKCNFCVHRLVKAEQK
AKTEKRSLRTDEVTTACAQACPTSAIVFGDLDDPNSAVSKLLAQAEQEGRVYRLLEDLGTDPKVYYLKKIDLEAAEHAAT
H

Sequences:

>Translated_241_residues
MPRWGMVIDLDRCIGCGACVVACQAENNVPINTEDVYHQRRAIAWIRLERYWEGEYPNIKARYIPVPCMQCTNAPCEPVC
PVYATYHTNEGINMQVYNRCIGTRYCAVNCHWHVRFFNFWEPKWPDSLKNQLNPDVTVRSRGVMEKCNFCVHRLVKAEQK
AKTEKRSLRTDEVTTACAQACPTSAIVFGDLDDPNSAVSKLLAQAEQEGRVYRLLEDLGTDPKVYYLKKIDLEAAEHAAT
H
>Mature_240_residues
PRWGMVIDLDRCIGCGACVVACQAENNVPINTEDVYHQRRAIAWIRLERYWEGEYPNIKARYIPVPCMQCTNAPCEPVCP
VYATYHTNEGINMQVYNRCIGTRYCAVNCHWHVRFFNFWEPKWPDSLKNQLNPDVTVRSRGVMEKCNFCVHRLVKAEQKA
KTEKRSLRTDEVTTACAQACPTSAIVFGDLDDPNSAVSKLLAQAEQEGRVYRLLEDLGTDPKVYYLKKIDLEAAEHAATH

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=224, Percent_Identity=35.7142857142857, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI226510944, Length=194, Percent_Identity=29.8969072164948, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787122, Length=204, Percent_Identity=23.5294117647059, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787872, Length=211, Percent_Identity=23.2227488151659, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27591; Mature: 27460

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRWGMVIDLDRCIGCGACVVACQAENNVPINTEDVYHQRRAIAWIRLERYWEGEYPNIK
CCCCCEEEEHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHEEEEEEHCCCCCCCCE
ARYIPVPCMQCTNAPCEPVCPVYATYHTNEGINMQVYNRCIGTRYCAVNCHWHVRFFNFW
EEEECCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEEEEEEEEEEECC
EPKWPDSLKNQLNPDVTVRSRGVMEKCNFCVHRLVKAEQKAKTEKRSLRTDEVTTACAQA
CCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
CPTSAIVFGDLDDPNSAVSKLLAQAEQEGRVYRLLEDLGTDPKVYYLKKIDLEAAEHAAT
CCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEECHHHHHHCCC
H
C
>Mature Secondary Structure 
PRWGMVIDLDRCIGCGACVVACQAENNVPINTEDVYHQRRAIAWIRLERYWEGEYPNIK
CCCCEEEEHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHEEEEEEHCCCCCCCCE
ARYIPVPCMQCTNAPCEPVCPVYATYHTNEGINMQVYNRCIGTRYCAVNCHWHVRFFNFW
EEEECCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEEEEEEEEEEECC
EPKWPDSLKNQLNPDVTVRSRGVMEKCNFCVHRLVKAEQKAKTEKRSLRTDEVTTACAQA
CCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
CPTSAIVFGDLDDPNSAVSKLLAQAEQEGRVYRLLEDLGTDPKVYYLKKIDLEAAEHAAT
CCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEECHHHHHHCCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]