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The map label for this gene is hpaB [H]

Identifier: 221632353

GI number: 221632353

Start: 328997

End: 329305

Strand: Reverse

Name: hpaB [H]

Synonym: trd_0324

Alternate gene names: 221632353

Gene position: 329305-328997 (Counterclockwise)

Preceding gene: 221632354

Following gene: 221632352

Centisome position: 16.44

GC content: 67.64

Gene sequence:

>309_bases
ATGGCGCAAGCAGTCGCCACCCGACCGTTTACGGGTGAGGAGTATCTGGAGAGCCTGCGGGACGGACGCGAGGTCTACGT
CTACGGTGAGCGCGTCACGGACGTCACGACCCATCCGGCGTTCCGCAACGCCGCCCGCATGGTCGCCCGGCTCTACGACG
CCTTGCACGATCCGGCCAAGAAGGACATCCTCACCGTCGAGACCGATACCGGCAACGGTGGCTTCACCCATCCCTTCTTC
CGCGCCCCCCCCGCTCGGTCGAGGACCTCGTCCGCGCCCGCGACGCCATCGCCGAATGGGCGCGTATGA

Upstream 100 bases:

>100_bases
TTTCCGGTTGATGATATTGCATGACAACCTGTAGACTTGACATCGGGACCCGCCAGACGAACGGATCGAAACCGAGGCTC
GCAGGCCACGGAGGTGGACT

Downstream 100 bases:

>100_bases
CCTACGGCTGGCTCGGGCGCAGCCCGGACTACAAGGCCAGCTTCCTCGGCACCCTCGGCGCCAACGCCCCCTTCTACGAG
CCGTTCAGCGCCAACGCCCG

Product: pyoverdin chromophore biosynthetic protein pvcC

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 102; Mature: 101

Protein sequence:

>102_residues
MAQAVATRPFTGEEYLESLRDGREVYVYGERVTDVTTHPAFRNAARMVARLYDALHDPAKKDILTVETDTGNGGFTHPFF
RAPPARSRTSSAPATPSPNGRV

Sequences:

>Translated_102_residues
MAQAVATRPFTGEEYLESLRDGREVYVYGERVTDVTTHPAFRNAARMVARLYDALHDPAKKDILTVETDTGNGGFTHPFF
RAPPARSRTSSAPATPSPNGRV
>Mature_101_residues
AQAVATRPFTGEEYLESLRDGREVYVYGERVTDVTTHPAFRNAARMVARLYDALHDPAKKDILTVETDTGNGGFTHPFFR
APPARSRTSSAPATPSPNGRV

Specific function: Utilizes FADH(2) supplied by hpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA. Can also oxidize phenol to catechol, and hydroxylate other phenol derivatives [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012688 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 11208; Mature: 11076

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAVATRPFTGEEYLESLRDGREVYVYGERVTDVTTHPAFRNAARMVARLYDALHDPAK
CCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
KDILTVETDTGNGGFTHPFFRAPPARSRTSSAPATPSPNGRV
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQAVATRPFTGEEYLESLRDGREVYVYGERVTDVTTHPAFRNAARMVARLYDALHDPAK
CCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
KDILTVETDTGNGGFTHPFFRAPPARSRTSSAPATPSPNGRV
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10653707 [H]