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The map label for this gene is hpaB [H]

Identifier: 221632352

GI number: 221632352

Start: 327744

End: 329000

Strand: Reverse

Name: hpaB [H]

Synonym: trd_0323

Alternate gene names: 221632352

Gene position: 329000-327744 (Counterclockwise)

Preceding gene: 221632353

Following gene: 221632351

Centisome position: 16.43

GC content: 64.76

Gene sequence:

>1257_bases
ATGACCTACGGCTGGCTCGGGCGCAGCCCGGACTACAAGGCCAGCTTCCTCGGCACCCTCGGCGCCAACGCCCCCTTCTA
CGAGCCGTTCAGCGCCAACGCCCGCCGCTGGTACCAGCTCGGCCAGGAACGGGTCCTCTACTGGAACCACGCCATCGTCA
ACCCGCCGGTCGACCGCAACCGCCCACCCGACGAGGTCGGCGATGTCTACATGCACGTCGAGCGGGAGACTGACGGCGGG
ATCTACGTCTCGGGCGCCAAGGTGGTCGCCACCGGCTCGGTGCTGACCCACTACAACTTCATCGCCCACTACGGTCCCTT
GCCGATCAAGGACAAGAAGTTCGCCCTCATCTTCACCGTCCCGATGGATGCACCGGGCGTCAAGCTGATCAGCCGGCCGA
GCTACGAGTACACCGCTGCTGTCATGGGCAGTCCCTTCGACTATCCACTCTCAAGTCGCCTGGACGAGAACGACGCCGTC
TTCATCTTCGACAATGTCTTCGTCCCCTGGGAGAACGTCTTCGTCTACGGCGATGTGGACAAGGTCAACAACTTCTTCCC
AGCCTCCGGCTTCATCCCGCGCTTCACCTTCCACAGCTGCACCCGCTTTGCCGTCAAGCTCGACTTCATCGCCGGGGTGC
TGCTCAAGGCCGTCGCCGCAACCGGCACCAAGGACTACCGGGGCGTGCAAGCCCAGGTCGGGGAGGTGATCGCCTGGCGC
AACCTGTTCTGGGCCATCACCGACGCCATGGCCCGCAACCCGATCCCCTGGACGGATGGCTACGTGCACCCGAACCTCGA
GTACGGCATGGCCTACCGCGTGCTGGCCCCGACCGCCTGGTCGCGTGTCCGCCAGATCATCCTGGACAATGTCGCGAGCG
GCCTCATTTACATCCCCAGCCATGCCCTGGACTTCCAGAACCCGGCCGAGCGCCCCTACCTCGAGAAGTACGTGCGGGGA
GCCGGTGGGATGGATGCGCACGACAGGGTCAAGCTCATGAAGCTCCTCTACGACGCGACCATCTCGGAGTTCGCCGGGCG
GCACGAACTCTACGAGCGGAACTACGCCGGTAACCACGAGAACATCCGCCTGGAGACGCTGATCGTCGCCGAAGCGGTCG
GCACCGCCGAGCGGATGCGCGGCTTCGCCGAGCAGTGCATGGCCGAATACGACCTGGAGGGATGGCTGACCCCGGACTTG
GTCAACAACGAGGACGTCTCCTTCGTCCTCCGGCGCTTCCGGGAGACGCAGGGGTAA

Upstream 100 bases:

>100_bases
ATACCGGCAACGGTGGCTTCACCCATCCCTTCTTCCGCGCCCCCCCCGCTCGGTCGAGGACCTCGTCCGCGCCCGCGACG
CCATCGCCGAATGGGCGCGT

Downstream 100 bases:

>100_bases
CCGTGGCCCCGCTCGTCGGGCCGGCAGCTCCGGCGGGTCTCCCGCCGGCCCGTGAGCGGCCGCCGGGAAGGAGGGTATGG
CCGCACGATGGGATCGGCAC

Product: pyoverdin chromophore biosynthetic protein pvcC

Products: NA

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTYGWLGRSPDYKASFLGTLGANAPFYEPFSANARRWYQLGQERVLYWNHAIVNPPVDRNRPPDEVGDVYMHVERETDGG
IYVSGAKVVATGSVLTHYNFIAHYGPLPIKDKKFALIFTVPMDAPGVKLISRPSYEYTAAVMGSPFDYPLSSRLDENDAV
FIFDNVFVPWENVFVYGDVDKVNNFFPASGFIPRFTFHSCTRFAVKLDFIAGVLLKAVAATGTKDYRGVQAQVGEVIAWR
NLFWAITDAMARNPIPWTDGYVHPNLEYGMAYRVLAPTAWSRVRQIILDNVASGLIYIPSHALDFQNPAERPYLEKYVRG
AGGMDAHDRVKLMKLLYDATISEFAGRHELYERNYAGNHENIRLETLIVAEAVGTAERMRGFAEQCMAEYDLEGWLTPDL
VNNEDVSFVLRRFRETQG

Sequences:

>Translated_418_residues
MTYGWLGRSPDYKASFLGTLGANAPFYEPFSANARRWYQLGQERVLYWNHAIVNPPVDRNRPPDEVGDVYMHVERETDGG
IYVSGAKVVATGSVLTHYNFIAHYGPLPIKDKKFALIFTVPMDAPGVKLISRPSYEYTAAVMGSPFDYPLSSRLDENDAV
FIFDNVFVPWENVFVYGDVDKVNNFFPASGFIPRFTFHSCTRFAVKLDFIAGVLLKAVAATGTKDYRGVQAQVGEVIAWR
NLFWAITDAMARNPIPWTDGYVHPNLEYGMAYRVLAPTAWSRVRQIILDNVASGLIYIPSHALDFQNPAERPYLEKYVRG
AGGMDAHDRVKLMKLLYDATISEFAGRHELYERNYAGNHENIRLETLIVAEAVGTAERMRGFAEQCMAEYDLEGWLTPDL
VNNEDVSFVLRRFRETQG
>Mature_417_residues
TYGWLGRSPDYKASFLGTLGANAPFYEPFSANARRWYQLGQERVLYWNHAIVNPPVDRNRPPDEVGDVYMHVERETDGGI
YVSGAKVVATGSVLTHYNFIAHYGPLPIKDKKFALIFTVPMDAPGVKLISRPSYEYTAAVMGSPFDYPLSSRLDENDAVF
IFDNVFVPWENVFVYGDVDKVNNFFPASGFIPRFTFHSCTRFAVKLDFIAGVLLKAVAATGTKDYRGVQAQVGEVIAWRN
LFWAITDAMARNPIPWTDGYVHPNLEYGMAYRVLAPTAWSRVRQIILDNVASGLIYIPSHALDFQNPAERPYLEKYVRGA
GGMDAHDRVKLMKLLYDATISEFAGRHELYERNYAGNHENIRLETLIVAEAVGTAERMRGFAEQCMAEYDLEGWLTPDLV
NNEDVSFVLRRFRETQG

Specific function: Utilizes FADH(2) supplied by hpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA. Can also oxidize phenol to catechol, and hydroxylate other phenol derivatives [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012688 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: NA

Molecular weight: Translated: 47307; Mature: 47176

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYGWLGRSPDYKASFLGTLGANAPFYEPFSANARRWYQLGQERVLYWNHAIVNPPVDRN
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEEECEEECCCCCCC
RPPDEVGDVYMHVERETDGGIYVSGAKVVATGSVLTHYNFIAHYGPLPIKDKKFALIFTV
CCCCCCCCEEEEEEEECCCCEEEECCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEEE
PMDAPGVKLISRPSYEYTAAVMGSPFDYPLSSRLDENDAVFIFDNVFVPWENVFVYGDVD
CCCCCCCEEEECCCCCEEEEEECCCCCCCHHCCCCCCCEEEEEECEECCCCCEEEEECHH
KVNNFFPASGFIPRFTFHSCTRFAVKLDFIAGVLLKAVAATGTKDYRGVQAQVGEVIAWR
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHH
NLFWAITDAMARNPIPWTDGYVHPNLEYGMAYRVLAPTAWSRVRQIILDNVASGLIYIPS
HHHHHHHHHHHCCCCCCCCCEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCEEEECC
HALDFQNPAERPYLEKYVRGAGGMDAHDRVKLMKLLYDATISEFAGRHELYERNYAGNHE
CCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NIRLETLIVAEAVGTAERMRGFAEQCMAEYDLEGWLTPDLVNNEDVSFVLRRFRETQG
CEEEEEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TYGWLGRSPDYKASFLGTLGANAPFYEPFSANARRWYQLGQERVLYWNHAIVNPPVDRN
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEEECEEECCCCCCC
RPPDEVGDVYMHVERETDGGIYVSGAKVVATGSVLTHYNFIAHYGPLPIKDKKFALIFTV
CCCCCCCCEEEEEEEECCCCEEEECCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEEE
PMDAPGVKLISRPSYEYTAAVMGSPFDYPLSSRLDENDAVFIFDNVFVPWENVFVYGDVD
CCCCCCCEEEECCCCCEEEEEECCCCCCCHHCCCCCCCEEEEEECEECCCCCEEEEECHH
KVNNFFPASGFIPRFTFHSCTRFAVKLDFIAGVLLKAVAATGTKDYRGVQAQVGEVIAWR
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHH
NLFWAITDAMARNPIPWTDGYVHPNLEYGMAYRVLAPTAWSRVRQIILDNVASGLIYIPS
HHHHHHHHHHHCCCCCCCCCEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCEEEECC
HALDFQNPAERPYLEKYVRGAGGMDAHDRVKLMKLLYDATISEFAGRHELYERNYAGNHE
CCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NIRLETLIVAEAVGTAERMRGFAEQCMAEYDLEGWLTPDLVNNEDVSFVLRRFRETQG
CEEEEEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10653707 [H]