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The map label for this gene is purB [H]

Identifier: 221230672

GI number: 221230672

Start: 2647448

End: 2648866

Strand: Reverse

Name: purB [H]

Synonym: MLBr_02230

Alternate gene names: 221230672

Gene position: 2648866-2647448 (Counterclockwise)

Preceding gene: 221230673

Following gene: 221230670

Centisome position: 81.05

GC content: 61.59

Gene sequence:

>1419_bases
GTGAGCATTCCGAATGTTCTAGCCGCCCGATACGCCAGCGCTGAATTGGTCGCGATATGGTCTCCGGAAGCCAGGGTTAT
CGCGGAGCGGCAGTTGTGGCTGGCCGTGCTACGAGCACAGGCGGAACTGAGTGTTCCGATGCCTGTTAAAGCGATCGCCG
ACTACGAGCGGGTGCTTGGCGAGGTCGATTTGGCTTCGATCGCCGCCCGCGAACGTGTGTTGCGTCACGACGTCAAGGCG
CGCATCGAGGAATTCAACGCGCTGGCCGGCCATGAGCAGGTACATAAGGGGATGACCAGCCGCGACTTGACCGAGAACGT
GGAGCAACTGCAGATCCGGCGGTCGTTGGAATTGGTTTTCTGCCATGGTGTGGCCGTAGTAGCACGGCTGGCCGAATGCG
CGGTGTCCTATCGCGACCTGGTGATGGCCGGACGCAGCCATAACGTTGCCGCGCAGGCCACGACATTGGGCAAACGCTTC
GCGTCCGCGACGCAGGAAACACTGATAGCGTTGACTAGGCTGCGCGAGTTGATCGACCGCTACCCACTGCGCGGTATCAA
GGGCCCGATGGGTACCGGGCAGGACATGCTCGACCTGCTTGGCGGTGACGCTGCGGCGCTAGCAGAACTTGAGCAAAGAA
TAGCTGAATTCCTAGGATTCACAAAGGTTTTAGCCAGTGTCGGGCAGGTATATCCGCGTTCACTAGACCACGATGTGATT
TCAGCGTTGGTGCAGCTCGGTGCCGGCTCTTCCTCGTTAGCGCACACCATACGGTTGATGGCAGGTCACGAGCTCGTTAC
CGAAGGCTTTGCACCGGGGCAGGTTGGCTCGTCAGCGATGCCACACAAGATGAACAGCCGCAGCTGTGAACGGGTCAACG
GTCTGCAGGTGGTGTTGCGCGGTTACGCCGCAATGGCAGCCGAACTAGCTGGCGCGCAGTGGAACGAGGGCGACGTTTTC
TGCTCAGTAGTACGTCGAGTTGCTTTGCCAGACAGCTTCTTTGCCATCGACGGCCAGATCGAGACGTTTTTGACGGTGCT
CGACGAGTTTGGTGCTTACCCGGCAGTGATCCGCCGCGAGCTGGACCGCTATTTGCCATTCCTGGCCACCACCAAGGTGC
TGATTGCGGCTGTGCGTGCTGGTATGGGTCGCGAGGCCGCTCACCACGTGATTCGCGAACACGCGGTGGCGACGGCGCTG
GCCATGCGGGAGTGCGGTGCCGAGCCTGACCTGCTGGACCGGTTGGCTGCCGATGGGCGGTTGCCGCTGGACCGAGCGGC
GCTAGACGCCGCGCTGGCCGACAAGAAGGCGTTCACCGGCGCCGCGGCAGACCAGGTAGACGAGGTTGTCGCGATGGTCG
ACAAGCTAGTGGGCCGCTACCCGGAAGCAGCCAAGTACACGCCGGGTGCTATCCTGTGA

Upstream 100 bases:

>100_bases
GGTTCACACCCGCAAAATACATCGGTCTATTAGAGTATCGGACGAGCTTGCATGGATGTTCCCGTTTTGCAGTGCGAACC
CGACCGTAAGCTGTGCTTCT

Downstream 100 bases:

>100_bases
CGCTGGTTGGCGCGCTTTCGGAGATCGACTTGACCTATACTGGATAACTTTGCTAGCGGCTTCTCGCATGAGTTGTTTGC
TATCCGTTGGCGGGAAGCGT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSIPNVLAARYASAELVAIWSPEARVIAERQLWLAVLRAQAELSVPMPVKAIADYERVLGEVDLASIAARERVLRHDVKA
RIEEFNALAGHEQVHKGMTSRDLTENVEQLQIRRSLELVFCHGVAVVARLAECAVSYRDLVMAGRSHNVAAQATTLGKRF
ASATQETLIALTRLRELIDRYPLRGIKGPMGTGQDMLDLLGGDAAALAELEQRIAEFLGFTKVLASVGQVYPRSLDHDVI
SALVQLGAGSSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNSRSCERVNGLQVVLRGYAAMAAELAGAQWNEGDVF
CSVVRRVALPDSFFAIDGQIETFLTVLDEFGAYPAVIRRELDRYLPFLATTKVLIAAVRAGMGREAAHHVIREHAVATAL
AMRECGAEPDLLDRLAADGRLPLDRAALDAALADKKAFTGAAADQVDEVVAMVDKLVGRYPEAAKYTPGAIL

Sequences:

>Translated_472_residues
MSIPNVLAARYASAELVAIWSPEARVIAERQLWLAVLRAQAELSVPMPVKAIADYERVLGEVDLASIAARERVLRHDVKA
RIEEFNALAGHEQVHKGMTSRDLTENVEQLQIRRSLELVFCHGVAVVARLAECAVSYRDLVMAGRSHNVAAQATTLGKRF
ASATQETLIALTRLRELIDRYPLRGIKGPMGTGQDMLDLLGGDAAALAELEQRIAEFLGFTKVLASVGQVYPRSLDHDVI
SALVQLGAGSSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNSRSCERVNGLQVVLRGYAAMAAELAGAQWNEGDVF
CSVVRRVALPDSFFAIDGQIETFLTVLDEFGAYPAVIRRELDRYLPFLATTKVLIAAVRAGMGREAAHHVIREHAVATAL
AMRECGAEPDLLDRLAADGRLPLDRAALDAALADKKAFTGAAADQVDEVVAMVDKLVGRYPEAAKYTPGAIL
>Mature_471_residues
SIPNVLAARYASAELVAIWSPEARVIAERQLWLAVLRAQAELSVPMPVKAIADYERVLGEVDLASIAARERVLRHDVKAR
IEEFNALAGHEQVHKGMTSRDLTENVEQLQIRRSLELVFCHGVAVVARLAECAVSYRDLVMAGRSHNVAAQATTLGKRFA
SATQETLIALTRLRELIDRYPLRGIKGPMGTGQDMLDLLGGDAAALAELEQRIAEFLGFTKVLASVGQVYPRSLDHDVIS
ALVQLGAGSSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNSRSCERVNGLQVVLRGYAAMAAELAGAQWNEGDVFC
SVVRRVALPDSFFAIDGQIETFLTVLDEFGAYPAVIRRELDRYLPFLATTKVLIAAVRAGMGREAAHHVIREHAVATALA
MRECGAEPDLLDRLAADGRLPLDRAALDAALADKKAFTGAAADQVDEVVAMVDKLVGRYPEAAKYTPGAIL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=442, Percent_Identity=32.579185520362, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=32.8083989501312, Blast_Score=204, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=28.7878787878788, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32564234, Length=343, Percent_Identity=30.0291545189504, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323391, Length=451, Percent_Identity=34.8115299334812, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24647570, Length=462, Percent_Identity=32.9004329004329, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50986; Mature: 50855

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPNVLAARYASAELVAIWSPEARVIAERQLWLAVLRAQAELSVPMPVKAIADYERVLG
CCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EVDLASIAARERVLRHDVKARIEEFNALAGHEQVHKGMTSRDLTENVEQLQIRRSLELVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
CHGVAVVARLAECAVSYRDLVMAGRSHNVAAQATTLGKRFASATQETLIALTRLRELIDR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPLRGIKGPMGTGQDMLDLLGGDAAALAELEQRIAEFLGFTKVLASVGQVYPRSLDHDVI
CCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
SALVQLGAGSSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNSRSCERVNGLQVVLR
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GYAAMAAELAGAQWNEGDVFCSVVRRVALPDSFFAIDGQIETFLTVLDEFGAYPAVIRRE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHH
LDRYLPFLATTKVLIAAVRAGMGREAAHHVIREHAVATALAMRECGAEPDLLDRLAADGR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
LPLDRAALDAALADKKAFTGAAADQVDEVVAMVDKLVGRYPEAAKYTPGAIL
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SIPNVLAARYASAELVAIWSPEARVIAERQLWLAVLRAQAELSVPMPVKAIADYERVLG
CCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EVDLASIAARERVLRHDVKARIEEFNALAGHEQVHKGMTSRDLTENVEQLQIRRSLELVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
CHGVAVVARLAECAVSYRDLVMAGRSHNVAAQATTLGKRFASATQETLIALTRLRELIDR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPLRGIKGPMGTGQDMLDLLGGDAAALAELEQRIAEFLGFTKVLASVGQVYPRSLDHDVI
CCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
SALVQLGAGSSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNSRSCERVNGLQVVLR
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GYAAMAAELAGAQWNEGDVFCSVVRRVALPDSFFAIDGQIETFLTVLDEFGAYPAVIRRE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHH
LDRYLPFLATTKVLIAAVRAGMGREAAHHVIREHAVATALAMRECGAEPDLLDRLAADGR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
LPLDRAALDAALADKKAFTGAAADQVDEVVAMVDKLVGRYPEAAKYTPGAIL
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]