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The map label for this gene is purD

Identifier: 221230673

GI number: 221230673

Start: 2653262

End: 2654530

Strand: Reverse

Name: purD

Synonym: MLBr_02235

Alternate gene names: 221230673

Gene position: 2654530-2653262 (Counterclockwise)

Preceding gene: 221230675

Following gene: 221230672

Centisome position: 81.23

GC content: 59.34

Gene sequence:

>1269_bases
GTGCGCGTCCTGGTGATTGGCTCCGGTGCCCGTGAGCATGCCTTGCTGCTGGCACTTAGCAGAGATCCGCAGGTCAAGGG
ATTAGTCGTTGCTCCCGGCAACGCTGGCACCGCCCGGCTGGCCGAACAGTATGACGTCGACATCAGCTCCGGAGAGGATA
TCGTCGCTCTGGCTCGTGATGTCCGGGCCGACATGGTGGTCATTGGTCCCGAGCTACCACTGGTGCTTGGAGTGGCCGAT
GCGGTGCGTGCTGCCGGCATCGTCTGTTTCGGTCCCAGTAAGGACGCTGCTCGCATCGAAGGCTCAAAAGCCTTCGCCAA
GGAAGTCATGGCGGCGGCTGGTGTGCGCACTTCCCGGAGCGAAATCGTCAATAGTCCAGCACGTCTGGATGCCGCCCTGG
ACCGGTTCGGGCCACCCGTCGGTGACTTGTCCTGGGTGGTGAAAGATGATCGTCTAGCGGCTGGCAAGGGCGTTGTGGTA
ACATCGGATCGCGATGTCGCGCGCACTCATGCTGCGGGATTATTAGAAGCCGGACACCCGGTATTGCTGGAGTCGTATCT
CGATGGACCCGAGGTGTCGCTATTCTGCGTCGTAGACGGTAGAACTGTGTTGCCGCTGTTACCGGCTCAAGATTTCAAGC
GGGTCGGTGAAGGCGACACAGGACCTAACACCGGCGGTATGGGCGCCTATGCTCCGCTGCCATGGCTTCCCGATGAGGTT
TGTCAACAGGCTCTCACCAGCATCGTCGAACCCGTTGCAGCCGAATTGGTTCAACGTGGAAGTCCGTTTCGCGGACTACT
GTATGTGGGTCTTGCTGTCACTGCCAGCGGGCCAGCAGTCGTTGAATTCAATTGCCGCTTTGGCGATCCGGAAACCCAAG
TTGTGCTGGCTCTGCTGGATTCACCGCTTGGTCAGCTCCTCTACGCGGCAGGAACTGGTTCGCTGGCTGACTTCGGCGAA
TTGCATTGGCGTTCAGGAGCTGCCGTGGCTGTAGTGTTAGCTGCCGAAAACTATCCCAGACGCCCCCGAGTCGGGGATAT
TGTCTTCGGATCCGAAATCGAGGGGGTATTGCACGCTGGAACCACTCGCCGCGACGATGGTGTGATCGTCTCGTCAGGTG
GGCGGGTGCTATCGGTGGTGGCGACCGGGGATGACCTATCTGCGGCGCGCTCCCAGGCATATCGCATAATCGGATCAATT
CGATTGCTCGGCAGCCATTTTCGCAAGGATATCGGATTTTCAGCGGCAAAGGGCAGGATCCATATCTAG

Upstream 100 bases:

>100_bases
TTTCTTTGTGGACGCTGCCGCCCTCGATCTCCTACGCGGCCGGGTCTGTTTGAATGGCCCGGCGCAACGGTCGAGTAATT
TCCAGCTAATCTGACGTGTC

Downstream 100 bases:

>100_bases
CAACGAAGTTAAGAGAAGTAGAGAAAAATGCTGCACTGTGCGTTGTTCGGTGGCAGATTATTAAGATAAAGCTTAGAAAG
CTGCGGCTTAGTTGTACTGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MRVLVIGSGAREHALLLALSRDPQVKGLVVAPGNAGTARLAEQYDVDISSGEDIVALARDVRADMVVIGPELPLVLGVAD
AVRAAGIVCFGPSKDAARIEGSKAFAKEVMAAAGVRTSRSEIVNSPARLDAALDRFGPPVGDLSWVVKDDRLAAGKGVVV
TSDRDVARTHAAGLLEAGHPVLLESYLDGPEVSLFCVVDGRTVLPLLPAQDFKRVGEGDTGPNTGGMGAYAPLPWLPDEV
CQQALTSIVEPVAAELVQRGSPFRGLLYVGLAVTASGPAVVEFNCRFGDPETQVVLALLDSPLGQLLYAAGTGSLADFGE
LHWRSGAAVAVVLAAENYPRRPRVGDIVFGSEIEGVLHAGTTRRDDGVIVSSGGRVLSVVATGDDLSAARSQAYRIIGSI
RLLGSHFRKDIGFSAAKGRIHI

Sequences:

>Translated_422_residues
MRVLVIGSGAREHALLLALSRDPQVKGLVVAPGNAGTARLAEQYDVDISSGEDIVALARDVRADMVVIGPELPLVLGVAD
AVRAAGIVCFGPSKDAARIEGSKAFAKEVMAAAGVRTSRSEIVNSPARLDAALDRFGPPVGDLSWVVKDDRLAAGKGVVV
TSDRDVARTHAAGLLEAGHPVLLESYLDGPEVSLFCVVDGRTVLPLLPAQDFKRVGEGDTGPNTGGMGAYAPLPWLPDEV
CQQALTSIVEPVAAELVQRGSPFRGLLYVGLAVTASGPAVVEFNCRFGDPETQVVLALLDSPLGQLLYAAGTGSLADFGE
LHWRSGAAVAVVLAAENYPRRPRVGDIVFGSEIEGVLHAGTTRRDDGVIVSSGGRVLSVVATGDDLSAARSQAYRIIGSI
RLLGSHFRKDIGFSAAKGRIHI
>Mature_422_residues
MRVLVIGSGAREHALLLALSRDPQVKGLVVAPGNAGTARLAEQYDVDISSGEDIVALARDVRADMVVIGPELPLVLGVAD
AVRAAGIVCFGPSKDAARIEGSKAFAKEVMAAAGVRTSRSEIVNSPARLDAALDRFGPPVGDLSWVVKDDRLAAGKGVVV
TSDRDVARTHAAGLLEAGHPVLLESYLDGPEVSLFCVVDGRTVLPLLPAQDFKRVGEGDTGPNTGGMGAYAPLPWLPDEV
CQQALTSIVEPVAAELVQRGSPFRGLLYVGLAVTASGPAVVEFNCRFGDPETQVVLALLDSPLGQLLYAAGTGSLADFGE
LHWRSGAAVAVVLAAENYPRRPRVGDIVFGSEIEGVLHAGTTRRDDGVIVSSGGRVLSVVATGDDLSAARSQAYRIIGSI
RLLGSHFRKDIGFSAAKGRIHI

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=433, Percent_Identity=39.2609699769053, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI4503915, Length=433, Percent_Identity=39.2609699769053, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI209869995, Length=433, Percent_Identity=39.2609699769053, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI209869993, Length=433, Percent_Identity=39.2609699769053, Blast_Score=297, Evalue=1e-80,
Organism=Escherichia coli, GI1790438, Length=430, Percent_Identity=42.3255813953488, Blast_Score=304, Evalue=8e-84,
Organism=Caenorhabditis elegans, GI17567511, Length=430, Percent_Identity=36.9767441860465, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6321203, Length=449, Percent_Identity=36.7483296213808, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI62471619, Length=435, Percent_Identity=39.3103448275862, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24582400, Length=435, Percent_Identity=39.0804597701149, Blast_Score=258, Evalue=6e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_MYCLE (Q50144)

Other databases:

- EMBL:   U15187
- EMBL:   Z95151
- EMBL:   AL583924
- PIR:   G87188
- RefSeq:   NP_302459.1
- ProteinModelPortal:   Q50144
- SMR:   Q50144
- EnsemblBacteria:   EBMYCT00000028171
- GeneID:   908797
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2235
- NMPDR:   fig|272631.1.peg.1331
- Leproma:   ML2235
- GeneTree:   EBGT00050000015554
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   MLEP272631:ML2235-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 43985; Mature: 43985

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVIGSGAREHALLLALSRDPQVKGLVVAPGNAGTARLAEQYDVDISSGEDIVALARD
CEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHH
VRADMVVIGPELPLVLGVADAVRAAGIVCFGPSKDAARIEGSKAFAKEVMAAAGVRTSRS
CCCCEEEECCCCCEEEEHHHHHHHCCEEEECCCCCCHHCCCHHHHHHHHHHHHCCCCCHH
EIVNSPARLDAALDRFGPPVGDLSWVVKDDRLAAGKGVVVTSDRDVARTHAAGLLEAGHP
HHHCCCHHHHHHHHHHCCCCCCCEEEEECCCEECCCCEEEECCCHHHHHHHHHHHHCCCC
VLLESYLDGPEVSLFCVVDGRTVLPLLPAQDFKRVGEGDTGPNTGGMGAYAPLPWLPDEV
EEEHHHCCCCCEEEEEEECCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
CQQALTSIVEPVAAELVQRGSPFRGLLYVGLAVTASGPAVVEFNCRFGDPETQVVLALLD
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHH
SPLGQLLYAAGTGSLADFGELHWRSGAAVAVVLAAENYPRRPRVGDIVFGSEIEGVLHAG
CCHHHHEEECCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCHHEECC
TTRRDDGVIVSSGGRVLSVVATGDDLSAARSQAYRIIGSIRLLGSHFRKDIGFSAAKGRI
CCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
HI
CC
>Mature Secondary Structure
MRVLVIGSGAREHALLLALSRDPQVKGLVVAPGNAGTARLAEQYDVDISSGEDIVALARD
CEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHH
VRADMVVIGPELPLVLGVADAVRAAGIVCFGPSKDAARIEGSKAFAKEVMAAAGVRTSRS
CCCCEEEECCCCCEEEEHHHHHHHCCEEEECCCCCCHHCCCHHHHHHHHHHHHCCCCCHH
EIVNSPARLDAALDRFGPPVGDLSWVVKDDRLAAGKGVVVTSDRDVARTHAAGLLEAGHP
HHHCCCHHHHHHHHHHCCCCCCCEEEEECCCEECCCCEEEECCCHHHHHHHHHHHHCCCC
VLLESYLDGPEVSLFCVVDGRTVLPLLPAQDFKRVGEGDTGPNTGGMGAYAPLPWLPDEV
EEEHHHCCCCCEEEEEEECCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
CQQALTSIVEPVAAELVQRGSPFRGLLYVGLAVTASGPAVVEFNCRFGDPETQVVLALLD
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHH
SPLGQLLYAAGTGSLADFGELHWRSGAAVAVVLAAENYPRRPRVGDIVFGSEIEGVLHAG
CCHHHHEEECCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCHHEECC
TTRRDDGVIVSSGGRVLSVVATGDDLSAARSQAYRIIGSIRLLGSHFRKDIGFSAAKGRI
CCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
HI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002