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The map label for this gene is gltX

Identifier: 221230374

GI number: 221230374

Start: 2033511

End: 2035019

Strand: Reverse

Name: gltX

Synonym: MLBr_01688

Alternate gene names: 221230374

Gene position: 2035019-2033511 (Counterclockwise)

Preceding gene: 221230375

Following gene: 221230373

Centisome position: 62.27

GC content: 58.78

Gene sequence:

>1509_bases
GTGACTGCAGTAACCTCTGATGGTACCCCGCAAGCAGCAAAAGTCAGGGTACGATTCTGCCCATCGCCCACTGGTGTTCC
GCACGTCGGGATGGTTCGCACGGCCCTGTTCAACTGGGCTTACGCTCGACATACTGGAGGCACCTTCGTCTTACGCATCG
AGGACACCGACGCCGACCGCGACAGTGAAGAAAGCTACCTGGCCCTGCTCGATGCGTTGCGCTGGCTCGGCCTGAACTGG
GACGAGGGTCCCGAGGTTGGAGGACCTTACGGTCCGTACCGGCAGTCGCAGCGCACTGACATCTACCGCGAGGTGGTGGC
AAAGTTGCTTGCTACAGGAGAGGCCTATTACGCTTTCTCCACACCGGAGGAAGTCGAGAACCGCCATCTTGCTGCTGGAC
GCAATCCCAAGCTGGGTTATGACAACTTTGACCGCGATCTTACCGATGCGCAGTTCTCAGCGTACTTGGCGGAAGGCCGT
AAGCCTGTAGTTCGGCTGCGGATGCCCGACGAGGATATCAGTTGGGATGACCTGGTGCGCGGGACAACTACCTTTGCTGT
GGGCACTGTGCCCGATTACGTGTTGACCCGAGCAAGCGGAGATCCGTTGTACACTTTGGTCAACCCGTGCGATGACGCGC
TGATGAAGATCACGCATGTACTGCGGGGCGAAGACTTACTATCGTCGACTCCGCGACAGGTGGCGCTATACCAGGCGTTG
ATCCGAATCGGGATGGCTGAGCGTATTCCCGAATTCGGTCATTTCCCATCGGTATTGGGGGAGGGAACCAAGAAACTCTC
GAAGCGAGAACCGCAGTCGAATTTGTTTGCCCACCGTGACCGGGGCTTTATCCCCGAAGGTCTGCTGAACTACCTTGCCT
TGCTGGGGTGGGCGATCGCCGATGACCACGACTTGTTCAGTCTCGATGAGATGGTAGCCGCGTTCGATGTGGTCGACGTC
AACTCCAATCCGGCGCGATTCGATCAGAAGAAGGCCGATGCGGTTAACGCCGAGCACATCCGAATGCTCGACTCCGAGGA
TTTCGCTGGCAGGCTACGCGACTACTTCACCACACACGGCTACCACATCGCTTTGGATCCCGCTAATTACGAAGCTGGTT
TCGTTGCCGCGGCGCAGTTGGTGCAGACCCGCATCGTTGTGCTTGGTGACGCTTGGGACCTGTTGAAATTCCTCAACGAT
GACGAGTACTCTATCGACTCTAAGGCCGCTGCCAAAGAACTGGATGCCGATGCCGGGCCGGTGCTTGATGTTGCCTGTGC
CGTGCTGGACAGCCTGGTGGATTGGACCACTGCGAGTATCGAGGACGTCCTCAAGGTTGCACTCATCGAAGGTCTTGGTC
TTAAACCGCGCAAAGTGTTTGGCCCGATCCGGGTCGCCGCCACGGGGGCATTGGTCAGCCCGCCGCTGTTCGAATCGCTG
GAGCTACTCGGCCGTGCTCGCAGTCTGCAGCGGCTGAGTGCTGCGCGAGCGCGAGTCACCTCGGCCTAG

Upstream 100 bases:

>100_bases
CACCGGCAGGTGTTGGTCCCGTCGAAGACGGTGACATCGTTTCTATCACTGTCGAAGGCATCGGAACTCTTACCAATCCC
GTAGTCCGCAAAGGAAAGTC

Downstream 100 bases:

>100_bases
CTGCAGGCCTAAGTCCGGTTGCGTGGCTGCTTACTCGCGCCGTTTCCAGCGCTCGCTATCGCCACACTAGGAACGCAACG
AGGTAAGACAAGGTGTGTGT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTAVTSDGTPQAAKVRVRFCPSPTGVPHVGMVRTALFNWAYARHTGGTFVLRIEDTDADRDSEESYLALLDALRWLGLNW
DEGPEVGGPYGPYRQSQRTDIYREVVAKLLATGEAYYAFSTPEEVENRHLAAGRNPKLGYDNFDRDLTDAQFSAYLAEGR
KPVVRLRMPDEDISWDDLVRGTTTFAVGTVPDYVLTRASGDPLYTLVNPCDDALMKITHVLRGEDLLSSTPRQVALYQAL
IRIGMAERIPEFGHFPSVLGEGTKKLSKREPQSNLFAHRDRGFIPEGLLNYLALLGWAIADDHDLFSLDEMVAAFDVVDV
NSNPARFDQKKADAVNAEHIRMLDSEDFAGRLRDYFTTHGYHIALDPANYEAGFVAAAQLVQTRIVVLGDAWDLLKFLND
DEYSIDSKAAAKELDADAGPVLDVACAVLDSLVDWTTASIEDVLKVALIEGLGLKPRKVFGPIRVAATGALVSPPLFESL
ELLGRARSLQRLSAARARVTSA

Sequences:

>Translated_502_residues
MTAVTSDGTPQAAKVRVRFCPSPTGVPHVGMVRTALFNWAYARHTGGTFVLRIEDTDADRDSEESYLALLDALRWLGLNW
DEGPEVGGPYGPYRQSQRTDIYREVVAKLLATGEAYYAFSTPEEVENRHLAAGRNPKLGYDNFDRDLTDAQFSAYLAEGR
KPVVRLRMPDEDISWDDLVRGTTTFAVGTVPDYVLTRASGDPLYTLVNPCDDALMKITHVLRGEDLLSSTPRQVALYQAL
IRIGMAERIPEFGHFPSVLGEGTKKLSKREPQSNLFAHRDRGFIPEGLLNYLALLGWAIADDHDLFSLDEMVAAFDVVDV
NSNPARFDQKKADAVNAEHIRMLDSEDFAGRLRDYFTTHGYHIALDPANYEAGFVAAAQLVQTRIVVLGDAWDLLKFLND
DEYSIDSKAAAKELDADAGPVLDVACAVLDSLVDWTTASIEDVLKVALIEGLGLKPRKVFGPIRVAATGALVSPPLFESL
ELLGRARSLQRLSAARARVTSA
>Mature_501_residues
TAVTSDGTPQAAKVRVRFCPSPTGVPHVGMVRTALFNWAYARHTGGTFVLRIEDTDADRDSEESYLALLDALRWLGLNWD
EGPEVGGPYGPYRQSQRTDIYREVVAKLLATGEAYYAFSTPEEVENRHLAAGRNPKLGYDNFDRDLTDAQFSAYLAEGRK
PVVRLRMPDEDISWDDLVRGTTTFAVGTVPDYVLTRASGDPLYTLVNPCDDALMKITHVLRGEDLLSSTPRQVALYQALI
RIGMAERIPEFGHFPSVLGEGTKKLSKREPQSNLFAHRDRGFIPEGLLNYLALLGWAIADDHDLFSLDEMVAAFDVVDVN
SNPARFDQKKADAVNAEHIRMLDSEDFAGRLRDYFTTHGYHIALDPANYEAGFVAAAQLVQTRIVVLGDAWDLLKFLNDD
EYSIDSKAAAKELDADAGPVLDVACAVLDSLVDWTTASIEDVLKVALIEGLGLKPRKVFGPIRVAATGALVSPPLFESLE
LLGRARSLQRLSAARARVTSA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=505, Percent_Identity=33.2673267326733, Blast_Score=219, Evalue=5e-57,
Organism=Escherichia coli, GI1788743, Length=492, Percent_Identity=34.349593495935, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI226510925, Length=255, Percent_Identity=30.1960784313725, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=29.8969072164948, Blast_Score=202, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=331, Percent_Identity=36.8580060422961, Blast_Score=202, Evalue=7e-53,
Organism=Drosophila melanogaster, GI24665321, Length=496, Percent_Identity=32.6612903225806, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_MYCLE (O33120)

Other databases:

- EMBL:   Z99263
- EMBL:   AL583923
- PIR:   T45422
- RefSeq:   NP_302160.1
- ProteinModelPortal:   O33120
- SMR:   O33120
- EnsemblBacteria:   EBMYCT00000028561
- GeneID:   910058
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1688
- NMPDR:   fig|272631.1.peg.1032
- Leproma:   ML1688
- GeneTree:   EBGT00050000016746
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   MLEP272631:ML1688-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55305; Mature: 55174

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 268-268

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVTSDGTPQAAKVRVRFCPSPTGVPHVGMVRTALFNWAYARHTGGTFVLRIEDTDADR
CCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
DSEESYLALLDALRWLGLNWDEGPEVGGPYGPYRQSQRTDIYREVVAKLLATGEAYYAFS
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEC
TPEEVENRHLAAGRNPKLGYDNFDRDLTDAQFSAYLAEGRKPVVRLRMPDEDISWDDLVR
CHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHC
GTTTFAVGTVPDYVLTRASGDPLYTLVNPCDDALMKITHVLRGEDLLSSTPRQVALYQAL
CCCEEEECCCCHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHH
IRIGMAERIPEFGHFPSVLGEGTKKLSKREPQSNLFAHRDRGFIPEGLLNYLALLGWAIA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
DDHDLFSLDEMVAAFDVVDVNSNPARFDQKKADAVNAEHIRMLDSEDFAGRLRDYFTTHG
CCCCHHHHHHHHHHHEEEECCCCCCCCCHHHHHCCCHHHHEECCCCHHHHHHHHHHHCCC
YHIALDPANYEAGFVAAAQLVQTRIVVLGDAWDLLKFLNDDEYSIDSKAAAKELDADAGP
EEEEECCCCCCHHHHHHHHHHHHHEEEEECHHHHHHHHCCCCCCCCHHHHHHHCCCCCCH
VLDVACAVLDSLVDWTTASIEDVLKVALIEGLGLKPRKVFGPIRVAATGALVSPPLFESL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEECCCCCCCHHHHHH
ELLGRARSLQRLSAARARVTSA
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAVTSDGTPQAAKVRVRFCPSPTGVPHVGMVRTALFNWAYARHTGGTFVLRIEDTDADR
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
DSEESYLALLDALRWLGLNWDEGPEVGGPYGPYRQSQRTDIYREVVAKLLATGEAYYAFS
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEC
TPEEVENRHLAAGRNPKLGYDNFDRDLTDAQFSAYLAEGRKPVVRLRMPDEDISWDDLVR
CHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHC
GTTTFAVGTVPDYVLTRASGDPLYTLVNPCDDALMKITHVLRGEDLLSSTPRQVALYQAL
CCCEEEECCCCHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHH
IRIGMAERIPEFGHFPSVLGEGTKKLSKREPQSNLFAHRDRGFIPEGLLNYLALLGWAIA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
DDHDLFSLDEMVAAFDVVDVNSNPARFDQKKADAVNAEHIRMLDSEDFAGRLRDYFTTHG
CCCCHHHHHHHHHHHEEEECCCCCCCCCHHHHHCCCHHHHEECCCCHHHHHHHHHHHCCC
YHIALDPANYEAGFVAAAQLVQTRIVVLGDAWDLLKFLNDDEYSIDSKAAAKELDADAGP
EEEEECCCCCCHHHHHHHHHHHHHEEEEECHHHHHHHHCCCCCCCCHHHHHHHCCCCCCH
VLDVACAVLDSLVDWTTASIEDVLKVALIEGLGLKPRKVFGPIRVAATGALVSPPLFESL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEECCCCCCCHHHHHH
ELLGRARSLQRLSAARARVTSA
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002