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The map label for this gene is recG [H]

Identifier: 221230362

GI number: 221230362

Start: 2014650

End: 2016881

Strand: Reverse

Name: recG [H]

Synonym: MLBr_01671

Alternate gene names: 221230362

Gene position: 2016881-2014650 (Counterclockwise)

Preceding gene: 221230363

Following gene: 221230360

Centisome position: 61.71

GC content: 59.86

Gene sequence:

>2232_bases
ATGGTGTCACTATCCGATCGGCTCGACTACATCGTGGGCGCCAAGGCTGCCGATTCCCTCGACGAAGTGTTTGGTATTCG
CACTGTCGACGACCTGCTACGCCATTACCCACGCAGTTACACTAAGGGTGCGACTGTGCGGGGCGCCCAAGACGAGCGGC
CGGAAGCAGGTGAACACATCACCATCGTCGACGTCATCACAGAGGCGGTGACGTTACCGATGAAGAAGGACTCGAAAAAG
AAGTATCTACGTCTCACGGTCGGCTCTGGACGCAACAAGGTGATCGCTACGTTTTTTAATGCGGGCTATATCAGTAAGGG
GCTAACCAAAGATACCCGGGTGATGCTCTCTGGGGAGGTTGGATTTTTTCGGGGCGTTATGCAGCTGACGCACCCCGCGT
TTCTCATTCTCGACTCGCCGGACGGACGCAACCGCGGCAGTAGCTCGCTGCGCCGCATCGCCGATGCTTCACAGGCTGTC
AGTGGCGAAGTGCTGATGTCGGCGTTCGAACGTCGATTCTTTCCGATCTATCCGGCCAGCACCAAACTGCAGAGCTGGGA
TATCTACGCCTGCGTGCGTCAGGTGCTTGAGGTCCTCGATCCGGTGGCCGATCCGTTGCCCGCGGATCTGCGCGCCAAGC
ATGGCCTGGTCTCCGAAGATGAGGCACTGCGCGCCATCCATCTCGCTGAAAGCGAGTCGGACCGTCGGCGTGCGCGGGAG
CGGCTAACCTTCGACGAAGCGGTCGGCTTGCAGTGGGCGTTGGTAACCCGAAGGCATGGTGAACTGTCGGAATCGGGACC
GTCGGCGCCGCCGCGGTCTGACGGTCTGATGGCAGAACTGATGCGGCGGTTGCCGTTCGAGCTTACGGAGGGACAGCGTG
AGGTGCGTGACGTGCTGTCCGATGGGCTAGCGGCTACCCGTCCGTTGAACCGCCTGCTGCAAGGTGAAGTCGGGTCGGGC
AAGACCATCGTCGCGGTATTGGCAATGTTGCAGATGATTGATGCTGGCTACCAATGCGTGTTGCTGGCTCCTACGGAAGT
TCTTGCTGCACAACATCTACTGTCGATCCGTGACGTTCTCGGCCCGTTGGGGATGGGGTGTCAGCTGGGCGGGGCGGAAA
ATGCCACCCAGGTGGCTCTGCTGACCGGCTCGATGACGATGGCCCAGAAGAAAAAGGTTCGCGCCGACATTTTCAGCGGC
CAGACTGGCATCGTCATCGGCACGCACGCGCTGCTGCAGGACGCGATTGAATTCCACAACCTAGGCATGGTAGTAGTCGA
CGAACAACACCGTTTCGGTGTCGAGCAGCGAGATCAATTGCGTACCAAGGCCCGCACTGGCATCATGCCACATCTGTTGG
TGATGACGGCGACGCCAATCCCGCGTACTGTCGCACTTACCGTCTATGGCGACTTGGAGATGTCGACACTGCGCGAACTG
CCGCGCGGACGTCAGCCGATAACCAGCAACGTCATCTTCGTCAAGGACAAGCCAGGGTGGCTGGACCGGGCTTGGCAGCG
CATCCTCGAGGAAGTCGCCGCGGGCCGCCAAGCGTATGTGGTGGCGCCCCGGATTGACGAGACCGAAGATCCGCAGAAAG
GGGGGCAAAACAGCAGACCATCGGAAACCGCGGAGGGTCTCTACGCACGATTGCGTTCTGGTGAGCTGGCTAACGTGCGG
CTGGCGCTTATGCACGGTCGGTTGTCCGCTGACGAGAAGGACGCTGCGATGATGGCTTTCCGTGCAGGCGAAATCGATGT
GCTGGTGTGTACCAACGTCATTGAGGTAGGCGTAGATGTACCTAACGCCACCATCATGCTGGTGATGGACGCCGATCGGT
TTGGAATAAGCCAGTTGCATCAGCTGCGTGGCCGCATCGGTCGCGGCACGCATCCGAGCCTGTGTTTGTTGGCCAGCTGG
GTCTCACCGGGCTCCCCGGCCGGGCGCAGGCTATGTGCCGTCGCCGAGACCATGGACGGGTTCGCACTCGCCGATCTCGA
CCTCAAGGAGCGTCGGGAAGGAGATGTTCTGGGCCGCAACCAATCCGGAAAGGCAATTACCCTGCGGATGCTGTCATTGG
CCGAGCATCAGGTGTTCATTGAGGCCGCAAGGGATTTCTGCACGCGTGCCTACGAGTATCCGCACCTCGGATTAGCCCCG
CATCCGGGATTGGCAGATTTGGCAGCACGATTCATTGACACCGACCGTATAGAATACTTAGGCAAGTCATGA

Upstream 100 bases:

>100_bases
TCCTGCAACAGTACGGGCACAACAACCATCCGGAGACTGAGTTGGTGACTTACCGCTGCGGGCATCGTGGTGATGCGTTG
CTGATCGGAGTCGAGTAGCT

Downstream 100 bases:

>100_bases
CGTCCAGTGACGATGCAGTGGGTGAAACCCTTCACAAGTGGGAAGTACCCCCCATCTGCTTGTGGCGGAGCGCGGAGCTG
GTGAATAGCTAAAGTGCTGC

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 743

Protein sequence:

>743_residues
MVSLSDRLDYIVGAKAADSLDEVFGIRTVDDLLRHYPRSYTKGATVRGAQDERPEAGEHITIVDVITEAVTLPMKKDSKK
KYLRLTVGSGRNKVIATFFNAGYISKGLTKDTRVMLSGEVGFFRGVMQLTHPAFLILDSPDGRNRGSSSLRRIADASQAV
SGEVLMSAFERRFFPIYPASTKLQSWDIYACVRQVLEVLDPVADPLPADLRAKHGLVSEDEALRAIHLAESESDRRRARE
RLTFDEAVGLQWALVTRRHGELSESGPSAPPRSDGLMAELMRRLPFELTEGQREVRDVLSDGLAATRPLNRLLQGEVGSG
KTIVAVLAMLQMIDAGYQCVLLAPTEVLAAQHLLSIRDVLGPLGMGCQLGGAENATQVALLTGSMTMAQKKKVRADIFSG
QTGIVIGTHALLQDAIEFHNLGMVVVDEQHRFGVEQRDQLRTKARTGIMPHLLVMTATPIPRTVALTVYGDLEMSTLREL
PRGRQPITSNVIFVKDKPGWLDRAWQRILEEVAAGRQAYVVAPRIDETEDPQKGGQNSRPSETAEGLYARLRSGELANVR
LALMHGRLSADEKDAAMMAFRAGEIDVLVCTNVIEVGVDVPNATIMLVMDADRFGISQLHQLRGRIGRGTHPSLCLLASW
VSPGSPAGRRLCAVAETMDGFALADLDLKERREGDVLGRNQSGKAITLRMLSLAEHQVFIEAARDFCTRAYEYPHLGLAP
HPGLADLAARFIDTDRIEYLGKS

Sequences:

>Translated_743_residues
MVSLSDRLDYIVGAKAADSLDEVFGIRTVDDLLRHYPRSYTKGATVRGAQDERPEAGEHITIVDVITEAVTLPMKKDSKK
KYLRLTVGSGRNKVIATFFNAGYISKGLTKDTRVMLSGEVGFFRGVMQLTHPAFLILDSPDGRNRGSSSLRRIADASQAV
SGEVLMSAFERRFFPIYPASTKLQSWDIYACVRQVLEVLDPVADPLPADLRAKHGLVSEDEALRAIHLAESESDRRRARE
RLTFDEAVGLQWALVTRRHGELSESGPSAPPRSDGLMAELMRRLPFELTEGQREVRDVLSDGLAATRPLNRLLQGEVGSG
KTIVAVLAMLQMIDAGYQCVLLAPTEVLAAQHLLSIRDVLGPLGMGCQLGGAENATQVALLTGSMTMAQKKKVRADIFSG
QTGIVIGTHALLQDAIEFHNLGMVVVDEQHRFGVEQRDQLRTKARTGIMPHLLVMTATPIPRTVALTVYGDLEMSTLREL
PRGRQPITSNVIFVKDKPGWLDRAWQRILEEVAAGRQAYVVAPRIDETEDPQKGGQNSRPSETAEGLYARLRSGELANVR
LALMHGRLSADEKDAAMMAFRAGEIDVLVCTNVIEVGVDVPNATIMLVMDADRFGISQLHQLRGRIGRGTHPSLCLLASW
VSPGSPAGRRLCAVAETMDGFALADLDLKERREGDVLGRNQSGKAITLRMLSLAEHQVFIEAARDFCTRAYEYPHLGLAP
HPGLADLAARFIDTDRIEYLGKS
>Mature_743_residues
MVSLSDRLDYIVGAKAADSLDEVFGIRTVDDLLRHYPRSYTKGATVRGAQDERPEAGEHITIVDVITEAVTLPMKKDSKK
KYLRLTVGSGRNKVIATFFNAGYISKGLTKDTRVMLSGEVGFFRGVMQLTHPAFLILDSPDGRNRGSSSLRRIADASQAV
SGEVLMSAFERRFFPIYPASTKLQSWDIYACVRQVLEVLDPVADPLPADLRAKHGLVSEDEALRAIHLAESESDRRRARE
RLTFDEAVGLQWALVTRRHGELSESGPSAPPRSDGLMAELMRRLPFELTEGQREVRDVLSDGLAATRPLNRLLQGEVGSG
KTIVAVLAMLQMIDAGYQCVLLAPTEVLAAQHLLSIRDVLGPLGMGCQLGGAENATQVALLTGSMTMAQKKKVRADIFSG
QTGIVIGTHALLQDAIEFHNLGMVVVDEQHRFGVEQRDQLRTKARTGIMPHLLVMTATPIPRTVALTVYGDLEMSTLREL
PRGRQPITSNVIFVKDKPGWLDRAWQRILEEVAAGRQAYVVAPRIDETEDPQKGGQNSRPSETAEGLYARLRSGELANVR
LALMHGRLSADEKDAAMMAFRAGEIDVLVCTNVIEVGVDVPNATIMLVMDADRFGISQLHQLRGRIGRGTHPSLCLLASW
VSPGSPAGRRLCAVAETMDGFALADLDLKERREGDVLGRNQSGKAITLRMLSLAEHQVFIEAARDFCTRAYEYPHLGLAP
HPGLADLAARFIDTDRIEYLGKS

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=718, Percent_Identity=32.1727019498607, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI1787357, Length=416, Percent_Identity=33.1730769230769, Blast_Score=202, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 81520; Mature: 81520

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLSDRLDYIVGAKAADSLDEVFGIRTVDDLLRHYPRSYTKGATVRGAQDERPEAGEHI
CCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCEE
TIVDVITEAVTLPMKKDSKKKYLRLTVGSGRNKVIATFFNAGYISKGLTKDTRVMLSGEV
EHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEECCCHHHCCCCCCCEEEEECCH
GFFRGVMQLTHPAFLILDSPDGRNRGSSSLRRIADASQAVSGEVLMSAFERRFFPIYPAS
HHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCC
TKLQSWDIYACVRQVLEVLDPVADPLPADLRAKHGLVSEDEALRAIHLAESESDRRRARE
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHH
RLTFDEAVGLQWALVTRRHGELSESGPSAPPRSDGLMAELMRRLPFELTEGQREVRDVLS
HCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHCCCHHHHHHHHH
DGLAATRPLNRLLQGEVGSGKTIVAVLAMLQMIDAGYQCVLLAPTEVLAAQHLLSIRDVL
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHH
GPLGMGCQLGGAENATQVALLTGSMTMAQKKKVRADIFSGQTGIVIGTHALLQDAIEFHN
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHC
LGMVVVDEQHRFGVEQRDQLRTKARTGIMPHLLVMTATPIPRTVALTVYGDLEMSTLREL
CCEEEEECHHHCCCCHHHHHHHHHHHCCCCEEHEEECCCCCCEEEEEEEECCCHHHHHHC
PRGRQPITSNVIFVKDKPGWLDRAWQRILEEVAAGRQAYVVAPRIDETEDPQKGGQNSRP
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCC
SETAEGLYARLRSGELANVRLALMHGRLSADEKDAAMMAFRAGEIDVLVCTNVIEVGVDV
CHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHEEECCCEEEEEECCHHHHCCCC
PNATIMLVMDADRFGISQLHQLRGRIGRGTHPSLCLLASWVSPGSPAGRRLCAVAETMDG
CCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCC
FALADLDLKERREGDVLGRNQSGKAITLRMLSLAEHQVFIEAARDFCTRAYEYPHLGLAP
CEEECCCHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HPGLADLAARFIDTDRIEYLGKS
CCCHHHHHHHHHCHHHHHHCCCC
>Mature Secondary Structure
MVSLSDRLDYIVGAKAADSLDEVFGIRTVDDLLRHYPRSYTKGATVRGAQDERPEAGEHI
CCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCEE
TIVDVITEAVTLPMKKDSKKKYLRLTVGSGRNKVIATFFNAGYISKGLTKDTRVMLSGEV
EHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEECCCHHHCCCCCCCEEEEECCH
GFFRGVMQLTHPAFLILDSPDGRNRGSSSLRRIADASQAVSGEVLMSAFERRFFPIYPAS
HHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCC
TKLQSWDIYACVRQVLEVLDPVADPLPADLRAKHGLVSEDEALRAIHLAESESDRRRARE
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHH
RLTFDEAVGLQWALVTRRHGELSESGPSAPPRSDGLMAELMRRLPFELTEGQREVRDVLS
HCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHCCCHHHHHHHHH
DGLAATRPLNRLLQGEVGSGKTIVAVLAMLQMIDAGYQCVLLAPTEVLAAQHLLSIRDVL
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHH
GPLGMGCQLGGAENATQVALLTGSMTMAQKKKVRADIFSGQTGIVIGTHALLQDAIEFHN
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHC
LGMVVVDEQHRFGVEQRDQLRTKARTGIMPHLLVMTATPIPRTVALTVYGDLEMSTLREL
CCEEEEECHHHCCCCHHHHHHHHHHHCCCCEEHEEECCCCCCEEEEEEEECCCHHHHHHC
PRGRQPITSNVIFVKDKPGWLDRAWQRILEEVAAGRQAYVVAPRIDETEDPQKGGQNSRP
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCC
SETAEGLYARLRSGELANVRLALMHGRLSADEKDAAMMAFRAGEIDVLVCTNVIEVGVDV
CHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHEEECCCEEEEEECCHHHHCCCC
PNATIMLVMDADRFGISQLHQLRGRIGRGTHPSLCLLASWVSPGSPAGRRLCAVAETMDG
CCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCC
FALADLDLKERREGDVLGRNQSGKAITLRMLSLAEHQVFIEAARDFCTRAYEYPHLGLAP
CEEECCCHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HPGLADLAARFIDTDRIEYLGKS
CCCHHHHHHHHHCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]