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The map label for this gene is nadE

Identifier: 221230242

GI number: 221230242

Start: 1759234

End: 1761276

Strand: Reverse

Name: nadE

Synonym: MLBr_01463

Alternate gene names: 221230242

Gene position: 1761276-1759234 (Counterclockwise)

Preceding gene: 221230243

Following gene: 221230241

Centisome position: 53.89

GC content: 58.2

Gene sequence:

>2043_bases
ATGGACTTCTACAACTCCTACAGCCAGGGTTTTGTCCGAGTCGCTGCGTGCACCCATCACGCTTCCATCGGCGATCCGAC
GACCAACGCTGCATCGGTTTTGCGGTTGGCCCGCCAGTGTCACGACGACGGAGTTGCCGTGGCCGTCTTCCCAGAGCTGA
CGTTGTCCGGTTACTCGATCGAGGATATTTTGCTCCAAGACCTTTTGCTCGAGGCTGTCGAGGACACCGTGCTCGATATC
GTTGTGGCCTCCGCGGATCTGTTGCCGGTTTTGGTCATTGGTGCCCCGCTGCGCTACCGGCATCGCATCTACAACACCGC
AGTGATCATCCACCGCGGTGTGGTACTCGGTGTGGCACCCAAGTCGTACCTGCCCACCTACCGGGAGTTCTACGAGCGTC
GTCAACTCGCACCTGGAGACGACGAACACGGCACCATTGGCATCGGTGACTTGCGGGCCCCGTTCGGTCCTGATCTGCTG
TTCGCCGCGGCCGACTTGCTCGGCTTGGTGCTGCACGTTGAAATCTGCGAGGACATGTTCGTGCCGGTGCCGCCCAGCGC
CGAAGCCGCGTTGGCAGGCGCGACGGTGTTGGCGAACCTGTCGGGCAGCCCGATCACGATCGGCCGCGCCGAAGACCGTC
GCCTGCTGGCCCGCTCGGCGTCGTTGCGGTGTCTAGCCGCTTACGTCTATGCCGCTGCGGGGGAGGGTGAGTCCACGACC
GACTTGGCTTGGGACGGTCAGACGATGATCTGGGAGAATGGGGTGCTGCTCGCGGAATCCGAGCGTTTCCCCAAGGGGGA
GCACCGCTCGGTCGCTGACGTCGACACCGAACTGCTTCGATCCGAGCGATTGCGCATGGGCACCTTCAACGACAACCGTC
GCCGTCACCGAGCGTTAGTTGAGCCATTCCGGCGCATTGAGTTTCGGCTCGAGCCGCCGGTCGGCAACATCGGATTGTTG
CGGGAGGTTGAGCGTTTTCCTTTTGTCCCAGCCGATCCGCAGCGTTTACAGCAAGATTGCTACGAGGCTTACAATATCCA
AGTTTCCGGGCTTGAACAACGGTTGCGTGCGTTGGACTACCCGAAGGTTGTAATCGGAGTTTCTGGCGGGCTAGATTCGA
CACATGCGCTGATCGTCGCCGCGCGCGCAATGGACCGGGAAGGACGCCCGCGCAGCGACATTCTCGCATTCACGCTGCCC
GGGTTCGTGACCGGAGATCGCACCAAAAGCAATGCCACCGAGCTGTGTCGCGCACTGGGTGTTACTTTCACGGAAATAGA
TATCAGAGACACCGCAACGTTGATGCTGAAAAAAATCGGTCATCCGTTCTCACGCGGCGAGGTTTCCTACGATGTCACTT
TCGAGAACGTCCAGGCAGGAGTGCGCACCGATTATCTCTTTCGGCTGGCCAACCAGCATGGTGGCATCGTGCTCGGCACC
GGTGACCTGTCAGAGCTGGGGCTGGGTTGGTCGACCTACGGTGTGGGTGATCAAATGTCCCACTATAATATCAATGCTGG
TGTTCCAAAGACATTGGTGCAACACCTGATCCGCTGGGTCATTGCCTCGAGTCAATTCGAGGAGCAGGTCGATAAGGTGC
TGCAGTCGGTGCTCGACACCGAGATCACTCCGGAACTTATTCCAAGCGATGGTGAAGAAAAACTTCAGAGCACTGAAGCA
AAGGTTGGTCCGTTTGCCCTACAAGATTTTTCGTTGTTTCAGGTCTTGCGGTACGGATTTCGTCCATCGAAGATCGCTTT
CCTGACCTGGCACGCTTGGAGCGATCCCAATTGCGGCAAGTGGCCACCTGGATTCCCAGAAGATAAACGATTGTCCTACT
CACTTAAGGAAATTAGGCATTGGCTGCAGATTTTTGTGCAACGTTTCTATTCGTTCAGTCAGTTCAAAAGATCAGCGCTG
CCCAATGGCCCCAAGGTGTCTCATGGAGGAGCGTTATCGCCGCGTGGGGATTGGCGAGCCCCGTCAGATATGTCCGCACG
GATCTGGCTCGACGAGATCGAGCGCGAAGTGCCCGAAGAGTAG

Upstream 100 bases:

>100_bases
ATAACGCTGGCATTATGCGGACGCCCGTACCGCACTGCCAGCTCATTGTGTCAACCCGGCGAATGCTTGGTGACACCAGT
CGGGTGACAATGAGAGGGCG

Downstream 100 bases:

>100_bases
CCGGTTACGGTGCGTCTTCCAGATCAGGAGGGCTTTCCTAGCTGGGCTACTTCGGGTCACTATTTGTCGGTGCTGTTAGT
GTCGAACCGCAGCCGTTAAA

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing]

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MDFYNSYSQGFVRVAACTHHASIGDPTTNAASVLRLARQCHDDGVAVAVFPELTLSGYSIEDILLQDLLLEAVEDTVLDI
VVASADLLPVLVIGAPLRYRHRIYNTAVIIHRGVVLGVAPKSYLPTYREFYERRQLAPGDDEHGTIGIGDLRAPFGPDLL
FAAADLLGLVLHVEICEDMFVPVPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASLRCLAAYVYAAAGEGESTT
DLAWDGQTMIWENGVLLAESERFPKGEHRSVADVDTELLRSERLRMGTFNDNRRRHRALVEPFRRIEFRLEPPVGNIGLL
REVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDYPKVVIGVSGGLDSTHALIVAARAMDREGRPRSDILAFTLP
GFVTGDRTKSNATELCRALGVTFTEIDIRDTATLMLKKIGHPFSRGEVSYDVTFENVQAGVRTDYLFRLANQHGGIVLGT
GDLSELGLGWSTYGVGDQMSHYNINAGVPKTLVQHLIRWVIASSQFEEQVDKVLQSVLDTEITPELIPSDGEEKLQSTEA
KVGPFALQDFSLFQVLRYGFRPSKIAFLTWHAWSDPNCGKWPPGFPEDKRLSYSLKEIRHWLQIFVQRFYSFSQFKRSAL
PNGPKVSHGGALSPRGDWRAPSDMSARIWLDEIEREVPEE

Sequences:

>Translated_680_residues
MDFYNSYSQGFVRVAACTHHASIGDPTTNAASVLRLARQCHDDGVAVAVFPELTLSGYSIEDILLQDLLLEAVEDTVLDI
VVASADLLPVLVIGAPLRYRHRIYNTAVIIHRGVVLGVAPKSYLPTYREFYERRQLAPGDDEHGTIGIGDLRAPFGPDLL
FAAADLLGLVLHVEICEDMFVPVPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASLRCLAAYVYAAAGEGESTT
DLAWDGQTMIWENGVLLAESERFPKGEHRSVADVDTELLRSERLRMGTFNDNRRRHRALVEPFRRIEFRLEPPVGNIGLL
REVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDYPKVVIGVSGGLDSTHALIVAARAMDREGRPRSDILAFTLP
GFVTGDRTKSNATELCRALGVTFTEIDIRDTATLMLKKIGHPFSRGEVSYDVTFENVQAGVRTDYLFRLANQHGGIVLGT
GDLSELGLGWSTYGVGDQMSHYNINAGVPKTLVQHLIRWVIASSQFEEQVDKVLQSVLDTEITPELIPSDGEEKLQSTEA
KVGPFALQDFSLFQVLRYGFRPSKIAFLTWHAWSDPNCGKWPPGFPEDKRLSYSLKEIRHWLQIFVQRFYSFSQFKRSAL
PNGPKVSHGGALSPRGDWRAPSDMSARIWLDEIEREVPEE
>Mature_680_residues
MDFYNSYSQGFVRVAACTHHASIGDPTTNAASVLRLARQCHDDGVAVAVFPELTLSGYSIEDILLQDLLLEAVEDTVLDI
VVASADLLPVLVIGAPLRYRHRIYNTAVIIHRGVVLGVAPKSYLPTYREFYERRQLAPGDDEHGTIGIGDLRAPFGPDLL
FAAADLLGLVLHVEICEDMFVPVPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASLRCLAAYVYAAAGEGESTT
DLAWDGQTMIWENGVLLAESERFPKGEHRSVADVDTELLRSERLRMGTFNDNRRRHRALVEPFRRIEFRLEPPVGNIGLL
REVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDYPKVVIGVSGGLDSTHALIVAARAMDREGRPRSDILAFTLP
GFVTGDRTKSNATELCRALGVTFTEIDIRDTATLMLKKIGHPFSRGEVSYDVTFENVQAGVRTDYLFRLANQHGGIVLGT
GDLSELGLGWSTYGVGDQMSHYNINAGVPKTLVQHLIRWVIASSQFEEQVDKVLQSVLDTEITPELIPSDGEEKLQSTEA
KVGPFALQDFSLFQVLRYGFRPSKIAFLTWHAWSDPNCGKWPPGFPEDKRLSYSLKEIRHWLQIFVQRFYSFSQFKRSAL
PNGPKVSHGGALSPRGDWRAPSDMSARIWLDEIEREVPEE

Specific function: Can use both glutamine or ammonia as a nitrogen source

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

Organism=Homo sapiens, GI41393551, Length=632, Percent_Identity=25, Blast_Score=82, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321865, Length=655, Percent_Identity=23.6641221374046, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24641841, Length=638, Percent_Identity=23.5109717868339, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24641843, Length=638, Percent_Identity=23.5109717868339, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADE_MYCLE (Q9CBZ6)

Other databases:

- EMBL:   AL583922
- PIR:   H87091
- RefSeq:   NP_302028.1
- ProteinModelPortal:   Q9CBZ6
- EnsemblBacteria:   EBMYCT00000028909
- GeneID:   909491
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1463
- NMPDR:   fig|272631.1.peg.900
- Leproma:   ML1463
- GeneTree:   EBGT00050000017830
- HOGENOM:   HBG732101
- OMA:   DGPKVGS
- ProtClustDB:   PRK02628
- BioCyc:   MLEP272631:ML1463-MONOMER
- BRENDA:   6.3.5.1
- HAMAP:   MF_00193
- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.60.110.10
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006630
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase; SSF56317 Ntlse/CNhydtse

EC number: =6.3.5.1

Molecular weight: Translated: 75544; Mature: 75544

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50263 CN_HYDROLASE; PS00591 GLYCOSYL_HYDROL_F10

Important sites: ACT_SITE 368-368

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFYNSYSQGFVRVAACTHHASIGDPTTNAASVLRLARQCHDDGVAVAVFPELTLSGYSI
CCCCCCHHCCEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCH
EDILLQDLLLEAVEDTVLDIVVASADLLPVLVIGAPLRYRHRIYNTAVIIHRGVVLGVAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEECCEEEEECC
KSYLPTYREFYERRQLAPGDDEHGTIGIGDLRAPFGPDLLFAAADLLGLVLHVEICEDMF
HHHCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
VPVPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASLRCLAAYVYAAAGEGESTT
CCCCCCCHHHHHHHHHEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DLAWDGQTMIWENGVLLAESERFPKGEHRSVADVDTELLRSERLRMGTFNDNRRRHRALV
EEEECCCEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EPFRRIEFRLEPPVGNIGLLREVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDY
HHHHHCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEECHHHHHHHCCC
PKVVIGVSGGLDSTHALIVAARAMDREGRPRSDILAFTLPGFVTGDRTKSNATELCRALG
CEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHC
VTFTEIDIRDTATLMLKKIGHPFSRGEVSYDVTFENVQAGVRTDYLFRLANQHGGIVLGT
CEEEEEECHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHCCCHHHHHHHHHCCCCCEEEEC
GDLSELGLGWSTYGVGDQMSHYNINAGVPKTLVQHLIRWVIASSQFEEQVDKVLQSVLDT
CCHHHHCCCCEECCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EITPELIPSDGEEKLQSTEAKVGPFALQDFSLFQVLRYGFRPSKIAFLTWHAWSDPNCGK
CCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCC
WPPGFPEDKRLSYSLKEIRHWLQIFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PSDMSARIWLDEIEREVPEE
CCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFYNSYSQGFVRVAACTHHASIGDPTTNAASVLRLARQCHDDGVAVAVFPELTLSGYSI
CCCCCCHHCCEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCH
EDILLQDLLLEAVEDTVLDIVVASADLLPVLVIGAPLRYRHRIYNTAVIIHRGVVLGVAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEECCEEEEECC
KSYLPTYREFYERRQLAPGDDEHGTIGIGDLRAPFGPDLLFAAADLLGLVLHVEICEDMF
HHHCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
VPVPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASLRCLAAYVYAAAGEGESTT
CCCCCCCHHHHHHHHHEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DLAWDGQTMIWENGVLLAESERFPKGEHRSVADVDTELLRSERLRMGTFNDNRRRHRALV
EEEECCCEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EPFRRIEFRLEPPVGNIGLLREVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDY
HHHHHCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEECHHHHHHHCCC
PKVVIGVSGGLDSTHALIVAARAMDREGRPRSDILAFTLPGFVTGDRTKSNATELCRALG
CEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHC
VTFTEIDIRDTATLMLKKIGHPFSRGEVSYDVTFENVQAGVRTDYLFRLANQHGGIVLGT
CEEEEEECHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHCCCHHHHHHHHHCCCCCEEEEC
GDLSELGLGWSTYGVGDQMSHYNINAGVPKTLVQHLIRWVIASSQFEEQVDKVLQSVLDT
CCHHHHCCCCEECCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EITPELIPSDGEEKLQSTEAKVGPFALQDFSLFQVLRYGFRPSKIAFLTWHAWSDPNCGK
CCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCC
WPPGFPEDKRLSYSLKEIRHWLQIFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PSDMSARIWLDEIEREVPEE
CCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002