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The map label for this gene is proB

Identifier: 221230243

GI number: 221230243

Start: 1761493

End: 1762596

Strand: Reverse

Name: proB

Synonym: MLBr_01464

Alternate gene names: 221230243

Gene position: 1762596-1761493 (Counterclockwise)

Preceding gene: 221230244

Following gene: 221230242

Centisome position: 53.93

GC content: 63.41

Gene sequence:

>1104_bases
GTGAGTGTACATCGCGACGCGATCAGAGCTGCTCGGAGTTTGGTCGTCAAGGTTGGGACTAACGCGCTCACCACATCATC
CGGAGTATTCGATTCTAGCCGGCTGGCCAGACTGGTCGACGCGATAGAGGCGCGGATGAAGGCCGGTACTGATGTGGTCA
TCGTGTCTTCGGGTGCCATCGCCGCCGGCATTGAGCCGCTTGGATTATCACTTCGCCCAAAAGATTTAGCGACCAAGCAG
GCTGCGGCCAGCGTCGGGCAAGTTGCGCTGGTAAACTCCTGGAGCGCAGCGTTCGCCCGGTATGGTCGCGCGGTAGGGCA
GGTGCTGCTGACTGCGCAGGATATTTCGATGCGAGTCCAACACACTAACGCTCAACGCACCCTAGACCGGTTGCGGGCGC
TTCATGCGGTGGCAATCGTCAACGAGAACGACACCGTGGCTACCAACGAGATCCGGTTCGGTGACAACGATCGGCTTTCG
GCGGTGGTGGCGCACCTGGTCGGTGCCGAAGCTTTAGTGCTGCTATCCGATATCAATGGTCTCTACGATTCGGATCCGCG
GAAGAATACCGGTGCGCGCTTTGTTCCTGAGGTGACCGGGTCGGCAGATCTCGATGGTGTGGTCGCCAGCCGGGGCAGTT
CCTTGGGAACAGGTGGAATGGTATCGAAGATGTCATCGGCGCTACTGGCCGCCGACGCGGGTGTGCCGGTGCTGCTGGCT
GCCGCGGCTGACGCCGCGACTGCGCTTACCGATGCCTCGGTTGGCACAGTGTTCGCCGCGCGGCCGGACCGGATGTCCGC
CCGGCGGTTCTGGTTGCGCTATGCTGCCGACTCTGTCGGCTCGTTAACCCTCGACGAGGGTGCGGTATGGGCAGTGGTAC
AGCAACGCCGCTCGCTGCTGGCCGCGGGTATCACCGCGGTTTCGGGCCGGTTCTACGGCGGTGATGTCGTGGAATTGCGC
GGACCAGACGCGACGATGGTCGCCCGCGGTGTGGTCGCCTATGACGCGACCGAGCTGGCCGCCATGATGGGCCGGTCGAC
CTCGGAGCTGCCTTGCGAGCTGCGCCGGCCCGCGGTGCACGCCGACGACCTCGTCTCGATTTGA

Upstream 100 bases:

>100_bases
AGGCCGGGGTACCGACGCGAGGTTGGAAAGTACCGACCCCGTTGGTACCGCTGAACGCAAGGTTGCGCGGCATCAGCGTC
ACAAACACGGTGGATGAGCC

Downstream 100 bases:

>100_bases
TGCGGGTCAGGGCGTCTTCGTTGCACCGAGATACATCTGCGCCGCAGATGATCTCGGCGTGGTTGCGGGACCAATACCAC
TGCGCGAGGGCGCGTAGTTT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSVHRDAIRAARSLVVKVGTNALTTSSGVFDSSRLARLVDAIEARMKAGTDVVIVSSGAIAAGIEPLGLSLRPKDLATKQ
AAASVGQVALVNSWSAAFARYGRAVGQVLLTAQDISMRVQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLS
AVVAHLVGAEALVLLSDINGLYDSDPRKNTGARFVPEVTGSADLDGVVASRGSSLGTGGMVSKMSSALLAADAGVPVLLA
AAADAATALTDASVGTVFAARPDRMSARRFWLRYAADSVGSLTLDEGAVWAVVQQRRSLLAAGITAVSGRFYGGDVVELR
GPDATMVARGVVAYDATELAAMMGRSTSELPCELRRPAVHADDLVSI

Sequences:

>Translated_367_residues
MSVHRDAIRAARSLVVKVGTNALTTSSGVFDSSRLARLVDAIEARMKAGTDVVIVSSGAIAAGIEPLGLSLRPKDLATKQ
AAASVGQVALVNSWSAAFARYGRAVGQVLLTAQDISMRVQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLS
AVVAHLVGAEALVLLSDINGLYDSDPRKNTGARFVPEVTGSADLDGVVASRGSSLGTGGMVSKMSSALLAADAGVPVLLA
AAADAATALTDASVGTVFAARPDRMSARRFWLRYAADSVGSLTLDEGAVWAVVQQRRSLLAAGITAVSGRFYGGDVVELR
GPDATMVARGVVAYDATELAAMMGRSTSELPCELRRPAVHADDLVSI
>Mature_366_residues
SVHRDAIRAARSLVVKVGTNALTTSSGVFDSSRLARLVDAIEARMKAGTDVVIVSSGAIAAGIEPLGLSLRPKDLATKQA
AASVGQVALVNSWSAAFARYGRAVGQVLLTAQDISMRVQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLSA
VVAHLVGAEALVLLSDINGLYDSDPRKNTGARFVPEVTGSADLDGVVASRGSSLGTGGMVSKMSSALLAADAGVPVLLAA
AADAATALTDASVGTVFAARPDRMSARRFWLRYAADSVGSLTLDEGAVWAVVQQRRSLLAAGITAVSGRFYGGDVVELRG
PDATMVARGVVAYDATELAAMMGRSTSELPCELRRPAVHADDLVSI

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=28.9115646258503, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=28.7671232876712, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=39.2857142857143, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17569967, Length=275, Percent_Identity=33.0909090909091, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17569969, Length=275, Percent_Identity=33.0909090909091, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320506, Length=403, Percent_Identity=31.5136476426799, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6321822, Length=356, Percent_Identity=33.4269662921348, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21357643, Length=289, Percent_Identity=33.9100346020761, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_MYCLB (B8ZRN2)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503659.1
- ProteinModelPortal:   B8ZRN2
- SMR:   B8ZRN2
- EnsemblBacteria:   EBMYCT00000086241
- GeneID:   7326895
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017499
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 38136; Mature: 38005

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVHRDAIRAARSLVVKVGTNALTTSSGVFDSSRLARLVDAIEARMKAGTDVVIVSSGAI
CCCCHHHHHHHHHEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
AAGIEPLGLSLRPKDLATKQAAASVGQVALVNSWSAAFARYGRAVGQVLLTAQDISMRVQ
EECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHEEEHHHEEEEE
HTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLSAVVAHLVGAEALVLLSDING
CCCHHHHHHHHHHHHHEEEECCCCCEEECEEEECCCCHHHHHHHHHHCHHHEEHHHHCCC
LYDSDPRKNTGARFVPEVTGSADLDGVVASRGSSLGTGGMVSKMSSALLAADAGVPVLLA
CCCCCCCCCCCCEECCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
AAADAATALTDASVGTVFAARPDRMSARRFWLRYAADSVGSLTLDEGAVWAVVQQRRSLL
EHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHH
AAGITAVSGRFYGGDVVELRGPDATMVARGVVAYDATELAAMMGRSTSELPCELRRPAVH
HHHHHHHCCCCCCCCEEEEECCCHHHHHCCHHHCCHHHHHHHHCCCCCCCCHHHCCCCCC
ADDLVSI
CHHHHCC
>Mature Secondary Structure 
SVHRDAIRAARSLVVKVGTNALTTSSGVFDSSRLARLVDAIEARMKAGTDVVIVSSGAI
CCCHHHHHHHHHEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
AAGIEPLGLSLRPKDLATKQAAASVGQVALVNSWSAAFARYGRAVGQVLLTAQDISMRVQ
EECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHEEEHHHEEEEE
HTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLSAVVAHLVGAEALVLLSDING
CCCHHHHHHHHHHHHHEEEECCCCCEEECEEEECCCCHHHHHHHHHHCHHHEEHHHHCCC
LYDSDPRKNTGARFVPEVTGSADLDGVVASRGSSLGTGGMVSKMSSALLAADAGVPVLLA
CCCCCCCCCCCCEECCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
AAADAATALTDASVGTVFAARPDRMSARRFWLRYAADSVGSLTLDEGAVWAVVQQRRSLL
EHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHH
AAGITAVSGRFYGGDVVELRGPDATMVARGVVAYDATELAAMMGRSTSELPCELRRPAVH
HHHHHHHCCCCCCCCEEEEECCCHHHHHCCHHHCCHHHHHHHHCCCCCCCCHHHCCCCCC
ADDLVSI
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA