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The map label for this gene is murE

Identifier: 221229906

GI number: 221229906

Start: 1076044

End: 1077636

Strand: Direct

Name: murE

Synonym: MLBr_00909

Alternate gene names: 221229906

Gene position: 1076044-1077636 (Clockwise)

Preceding gene: 221229905

Following gene: 221229907

Centisome position: 32.93

GC content: 65.03

Gene sequence:

>1593_bases
GTGACTGAGGTGGTGGCGGTGCCGGTTAGGTTGCGCCCCAGCGCCACTGCGGGGGTGCGGCTCCCTGAGCTTGTGGCTCA
GGTTGGTGCTGTGCTGGCCGACGGCCCCGGGCAGGCGGCGACTGTCCCAGACATTCCGGTGACCGGTGTGACGCTGCGGG
CCCAGGAGGTGCTGTCCGGTGATCTGTTCGCTGCATTGGCTGGTGCATCCACACACGGCGCTCGGTATGCCGGCGTTGCG
CTCGAACGAGGTGCTGTCGCGGTGCTTACCGACGTTGCAGGGGTCGCCGAGCTGACAGCCCAGGCCAGCAGCGTGCCGAT
ACTCATACATCCGGAACCCCGCAGCGTGCTCGGCGGTCTTGCCGCCGCGGCATATGGACATCCCTCCAACCGGATGACGG
TTGTTGGGATTACTGGAACATCGGGTAAGACGACTACCACCTATATGGTTGAGGCTGGTTTACGGGCTGGTGGGCGAGTG
GTCGGGCTAGTCGGCACCATCGGCATTCGGATCGACGGTGCCGACATCCCTAGCTTTCTGACCACGCCGGAGGCTCCTGC
GCTGCAGGCGATGCTCGCGGCGATGGTCGAGCGCGGTGTGGAAACCGTGGTTATGGAGGTGTCTAGTCACGCGTTGAGTC
TGGGTCGGGTGGAAGGCACCCAGTTCGCAGTGGCCGGTTTCACCAATTTGTCTCGCGACCACCTCGATTTTCACCCCGAC
ATGGAGGAGTATTTCGAAGCCAAGGCGGTGCTGTTCGATCCGCACTCGTTGCTGCGGGCTCGCACCGTCGTGGTATGCAT
CGACGATGACGCTGGGCGCGCGACGGCCGCCCGGGCCGGTGACGCCATCACAGTCAGCGCCCTAGGTCAACCCGCATCGT
GGCGCGCGACGGACATTAGGTCACCGGGTGTCGGAGCCCAGGAGTTCACCGCCGTAGACCCCGCCGGTGTCCAGCACCGG
GTCGGCATTCGGTTACCCGGCCGCTACAACGTCGCTAATTGTCTTGTCGCCCTGGCGATTCTAGACGTTGTTGGGGTGTC
TCCGGAGCAGGCTTCCTTGGGGTTCCGGGACATCCGAATCCCCGGACGACTGGAACGGATCGACTGCGGCCAAGATTTTC
TTGCACTGGTCGACTATGCGCACAAGCCAGGAGCGTTACACTCAGTGTTGACCGCCCTACTGCAGCCGGACCATCGGCTG
GCAGTGGTGTTCGGTGCGGGGGGCGAGCGTGACCCGGGCAAGCGGGCTCCGATGGGCGAGATTGCCGCTGAGCTAGCCGA
TTTGGTCGTCGTGACCGATGACAACCCGCGTGGCGAGGATCCGGCGGCAATTCGTCGTGACATTCTGACCGGCACTGTCG
CGGCTGGCGGTGCGGCGCAGGTCGTCGAGATTGGTGACCGGCGGGCTGCTATCCAATACGCGGTTGCTTGGGCGGGTCCT
GATGACGTGGTGTTGGTGGCGGGCAAAGGTCATGAAACCGGGCAGCGGGGCGCTGCCGAGACCTGCCCCTTCGACGACCG
GGTGGAGCTGGCTCGGGCGCTGCAGGTCCGTGACGCCCGACTGCTTCCTGCGCCTGGGCGAGCGTGCCAATGA

Upstream 100 bases:

>100_bases
GTCTACTCGCTAATTTTCGCTTTAGCGGCGATTTCGCCGCCCTGTGCTGGAGCCGGTGTGTCCGGGTAGGGTGTCATGGC
TAGTCATGGTTGACGGAGGT

Downstream 100 bases:

>100_bases
TCGACCTGACGGTTGCCCGGGTTACCGAGATCGTCGGTGGCGCGTTGGTCGATATCTCTCCCGAGGAGGCCGCCGAGCGT
CGCGTCACCGGCAGCGTGGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTEVVAVPVRLRPSATAGVRLPELVAQVGAVLADGPGQAATVPDIPVTGVTLRAQEVLSGDLFAALAGASTHGARYAGVA
LERGAVAVLTDVAGVAELTAQASSVPILIHPEPRSVLGGLAAAAYGHPSNRMTVVGITGTSGKTTTTYMVEAGLRAGGRV
VGLVGTIGIRIDGADIPSFLTTPEAPALQAMLAAMVERGVETVVMEVSSHALSLGRVEGTQFAVAGFTNLSRDHLDFHPD
MEEYFEAKAVLFDPHSLLRARTVVVCIDDDAGRATAARAGDAITVSALGQPASWRATDIRSPGVGAQEFTAVDPAGVQHR
VGIRLPGRYNVANCLVALAILDVVGVSPEQASLGFRDIRIPGRLERIDCGQDFLALVDYAHKPGALHSVLTALLQPDHRL
AVVFGAGGERDPGKRAPMGEIAAELADLVVVTDDNPRGEDPAAIRRDILTGTVAAGGAAQVVEIGDRRAAIQYAVAWAGP
DDVVLVAGKGHETGQRGAAETCPFDDRVELARALQVRDARLLPAPGRACQ

Sequences:

>Translated_530_residues
MTEVVAVPVRLRPSATAGVRLPELVAQVGAVLADGPGQAATVPDIPVTGVTLRAQEVLSGDLFAALAGASTHGARYAGVA
LERGAVAVLTDVAGVAELTAQASSVPILIHPEPRSVLGGLAAAAYGHPSNRMTVVGITGTSGKTTTTYMVEAGLRAGGRV
VGLVGTIGIRIDGADIPSFLTTPEAPALQAMLAAMVERGVETVVMEVSSHALSLGRVEGTQFAVAGFTNLSRDHLDFHPD
MEEYFEAKAVLFDPHSLLRARTVVVCIDDDAGRATAARAGDAITVSALGQPASWRATDIRSPGVGAQEFTAVDPAGVQHR
VGIRLPGRYNVANCLVALAILDVVGVSPEQASLGFRDIRIPGRLERIDCGQDFLALVDYAHKPGALHSVLTALLQPDHRL
AVVFGAGGERDPGKRAPMGEIAAELADLVVVTDDNPRGEDPAAIRRDILTGTVAAGGAAQVVEIGDRRAAIQYAVAWAGP
DDVVLVAGKGHETGQRGAAETCPFDDRVELARALQVRDARLLPAPGRACQ
>Mature_529_residues
TEVVAVPVRLRPSATAGVRLPELVAQVGAVLADGPGQAATVPDIPVTGVTLRAQEVLSGDLFAALAGASTHGARYAGVAL
ERGAVAVLTDVAGVAELTAQASSVPILIHPEPRSVLGGLAAAAYGHPSNRMTVVGITGTSGKTTTTYMVEAGLRAGGRVV
GLVGTIGIRIDGADIPSFLTTPEAPALQAMLAAMVERGVETVVMEVSSHALSLGRVEGTQFAVAGFTNLSRDHLDFHPDM
EEYFEAKAVLFDPHSLLRARTVVVCIDDDAGRATAARAGDAITVSALGQPASWRATDIRSPGVGAQEFTAVDPAGVQHRV
GIRLPGRYNVANCLVALAILDVVGVSPEQASLGFRDIRIPGRLERIDCGQDFLALVDYAHKPGALHSVLTALLQPDHRLA
VVFGAGGERDPGKRAPMGEIAAELADLVVVTDDNPRGEDPAAIRRDILTGTVAAGGAAQVVEIGDRRAAIQYAVAWAGPD
DVVLVAGKGHETGQRGAAETCPFDDRVELARALQVRDARLLPAPGRACQ

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=493, Percent_Identity=38.9452332657201, Blast_Score=224, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_MYCLE (O69557)

Other databases:

- EMBL:   AL022602
- EMBL:   AL583920
- PIR:   G87022
- RefSeq:   NP_301692.1
- ProteinModelPortal:   O69557
- SMR:   O69557
- EnsemblBacteria:   EBMYCT00000029006
- GeneID:   909910
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0909
- NMPDR:   fig|272631.1.peg.564
- Leproma:   ML0909
- GeneTree:   EBGT00050000015779
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   PRK00139
- BioCyc:   MLEP272631:ML0909-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54809; Mature: 54678

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 208-208 BINDING 216-216 BINDING 410-410 BINDING 488-488 BINDING 492-492

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVVAVPVRLRPSATAGVRLPELVAQVGAVLADGPGQAATVPDIPVTGVTLRAQEVLSG
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEHHHHHCC
DLFAALAGASTHGARYAGVALERGAVAVLTDVAGVAELTAQASSVPILIHPEPRSVLGGL
HHHHHHHCCCCCCCEEECEEECCCCEEEEHHHHHHHHHHHHCCCCCEEECCCCHHHHHHH
AAAAYGHPSNRMTVVGITGTSGKTTTTYMVEAGLRAGGRVVGLVGTIGIRIDGADIPSFL
HHHHCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEEEEEEEEECCCCCHHH
TTPEAPALQAMLAAMVERGVETVVMEVSSHALSLGRVEGTQFAVAGFTNLSRDHLDFHPD
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
MEEYFEAKAVLFDPHSLLRARTVVVCIDDDAGRATAARAGDAITVSALGQPASWRATDIR
HHHHHCCCEEEECCHHHHCEEEEEEEEECCCCCCHHHCCCCEEEEEECCCCCCCCCCCCC
SPGVGAQEFTAVDPAGVQHRVGIRLPGRYNVANCLVALAILDVVGVSPEQASLGFRDIRI
CCCCCHHHEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCEEEEC
PGRLERIDCGQDFLALVDYAHKPGALHSVLTALLQPDHRLAVVFGAGGERDPGKRAPMGE
CCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHH
IAAELADLVVVTDDNPRGEDPAAIRRDILTGTVAAGGAAQVVEIGDRRAAIQYAVAWAGP
HHHHHEEEEEEECCCCCCCCHHHHHHHHHHEEECCCCCEEEEEECCCCEEEEEEEEECCC
DDVVLVAGKGHETGQRGAAETCPFDDRVELARALQVRDARLLPAPGRACQ
CCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCC
>Mature Secondary Structure 
TEVVAVPVRLRPSATAGVRLPELVAQVGAVLADGPGQAATVPDIPVTGVTLRAQEVLSG
CCEEEEEEEECCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEHHHHHCC
DLFAALAGASTHGARYAGVALERGAVAVLTDVAGVAELTAQASSVPILIHPEPRSVLGGL
HHHHHHHCCCCCCCEEECEEECCCCEEEEHHHHHHHHHHHHCCCCCEEECCCCHHHHHHH
AAAAYGHPSNRMTVVGITGTSGKTTTTYMVEAGLRAGGRVVGLVGTIGIRIDGADIPSFL
HHHHCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEEEEEEEEECCCCCHHH
TTPEAPALQAMLAAMVERGVETVVMEVSSHALSLGRVEGTQFAVAGFTNLSRDHLDFHPD
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
MEEYFEAKAVLFDPHSLLRARTVVVCIDDDAGRATAARAGDAITVSALGQPASWRATDIR
HHHHHCCCEEEECCHHHHCEEEEEEEEECCCCCCHHHCCCCEEEEEECCCCCCCCCCCCC
SPGVGAQEFTAVDPAGVQHRVGIRLPGRYNVANCLVALAILDVVGVSPEQASLGFRDIRI
CCCCCHHHEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCEEEEC
PGRLERIDCGQDFLALVDYAHKPGALHSVLTALLQPDHRLAVVFGAGGERDPGKRAPMGE
CCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHH
IAAELADLVVVTDDNPRGEDPAAIRRDILTGTVAAGGAAQVVEIGDRRAAIQYAVAWAGP
HHHHHEEEEEEECCCCCCCCHHHHHHHHHHEEECCCCCEEEEEECCCCEEEEEEEEECCC
DDVVLVAGKGHETGQRGAAETCPFDDRVELARALQVRDARLLPAPGRACQ
CCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002