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The map label for this gene is murF

Identifier: 221229907

GI number: 221229907

Start: 1077633

End: 1079186

Strand: Direct

Name: murF

Synonym: MLBr_00910

Alternate gene names: 221229907

Gene position: 1077633-1079186 (Clockwise)

Preceding gene: 221229906

Following gene: 221229908

Centisome position: 32.97

GC content: 63.58

Gene sequence:

>1554_bases
ATGATCGACCTGACGGTTGCCCGGGTTACCGAGATCGTCGGTGGCGCGTTGGTCGATATCTCTCCCGAGGAGGCCGCCGA
GCGTCGCGTCACCGGCAGCGTGGAATTTGATTCGCGGGCTGTTGGCCCCGGCGGTCTATTCCTGGCCTTGCCGGGGGCCC
GCTCGGACGGACATGACCATGCTGCTTCGGCGATAGCGGCCGGTGCGGTGGCTGTGTTGGCTGCCCGGCCGGTCGGCGTT
CCCGCCATCGTAGTTGCTCCTGACCCTCGCACGGGTGACGGCGGGCTGACTGGGGTGCTTGAGCACGATGCAGACGGATC
GGGCGCGGCGGTGCTGGCCGCCTTGGCTAAGTTGGCCAAGGCGGTGGCCGCGGAGCTGGTAGCTGGCGGGCTGACTATCA
TTGGGATTACCGGATCGTCGGGGAAGACATCCACCAAAGATTTGGTGGCCGTCGTGCTGGAGTTGCTCGGTGAGGTGGTG
GCGCCACCCGAGTCGTTCAACAATGAACTGGGTCATCCCTGGACAGTGTTGCGTGCAACCCGTAGCACGGACTATTTGAT
TCTGGAGATGTCGGCACGCCGTCCCGGTAATATTGCCGCGTTGGCGGCGATCGCCCCACCAAAGATAGGGGTTGTCCTCA
ACGTCGGCACTGCGCACCTAGGTGAGTTTGGCTCCCGGGAAGCCATCGCGCGAACTAAAACCGAGCTTCCGCAAGCTATT
ATGCAGTCCGGTGTGGTCATACTCAACGTCGATGACCCGGCGGTGGCGGCGATGGCTGACGCGACCGTGGCCCGCGTGAT
TCGGGTTAGTCGCGGTAGTTATAGCCATCCGGGGTCACCAAACAGCCCAGACGTTTGGACTGGTCCGGTGTCGCTCGACG
AACTTGCTAGGCCGCGCTTCACGCTGCATACTCGAGATCCACGCGCCGGCGCCACCGAAATCCAACTCGGCGTGTACGGC
GATCATCAGGTCGCCAACGCCTTGTGCGCGGCTGCGGTCGGACTGGAATGCGGTGCTAGCGTCGAGGAAGTGGCGGTCGC
GCTTGCCGCGACAGGGCCGGTGTCGCGGCATCGGATGCAGGTGACCACGCGCGCTGACGGGGTGACGGTGATCGACGACA
CCTACAACGCCAACCCCGACTCGATGCGGGCCGGGCTACAGGCTTTGGCCTGGATCGCGCATGGTGGGACCCATGATAAG
AACCAGCCAGGTTCTTGTGCTCGACGAAGCTGGGCGGTGTTGGGTGAGATGGCCGAGCTTGGAGAGGACTCGATAACCGA
ACACGATCGCATCGGCCGGCTAGCGGTGCGCTTAGATGTGTCTCGACTCGTTGTCGTGGGAGGGGGAAGGTCGATAAACG
CCATGCACCGCGGAGCGGTTATGGAAGGTTCTTGGGGTTTAGAGACGGTTAATGTGGCCGACCCCGCTGCTGCCCTGACA
TTGTTGCGGGCTGAAGTACGACCCGGCGACGTGGTCCTGATCAAGGCGTCGCATTCGGTCGGCCTAGGAGCGCTGGCTGA
TGTACTGGTGGGCGACGGGGCCATCCGCCCATGA

Upstream 100 bases:

>100_bases
GCGCTGCCGAGACCTGCCCCTTCGACGACCGGGTGGAGCTGGCTCGGGCGCTGCAGGTCCGTGACGCCCGACTGCTTCCT
GCGCCTGGGCGAGCGTGCCA

Downstream 100 bases:

>100_bases
GACAGATCCTTGTCGCTGTCACAGTCGCGCTGGTTGTGTCTATCCTGTTGACCCCAGCGCTGATCCGGTTGTTCACCAGA
CACGGCTTCGGCCAGGAAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase (D-alanine:D-alanine-adding enzyme)

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MIDLTVARVTEIVGGALVDISPEEAAERRVTGSVEFDSRAVGPGGLFLALPGARSDGHDHAASAIAAGAVAVLAARPVGV
PAIVVAPDPRTGDGGLTGVLEHDADGSGAAVLAALAKLAKAVAAELVAGGLTIIGITGSSGKTSTKDLVAVVLELLGEVV
APPESFNNELGHPWTVLRATRSTDYLILEMSARRPGNIAALAAIAPPKIGVVLNVGTAHLGEFGSREAIARTKTELPQAI
MQSGVVILNVDDPAVAAMADATVARVIRVSRGSYSHPGSPNSPDVWTGPVSLDELARPRFTLHTRDPRAGATEIQLGVYG
DHQVANALCAAAVGLECGASVEEVAVALAATGPVSRHRMQVTTRADGVTVIDDTYNANPDSMRAGLQALAWIAHGGTHDK
NQPGSCARRSWAVLGEMAELGEDSITEHDRIGRLAVRLDVSRLVVVGGGRSINAMHRGAVMEGSWGLETVNVADPAAALT
LLRAEVRPGDVVLIKASHSVGLGALADVLVGDGAIRP

Sequences:

>Translated_517_residues
MIDLTVARVTEIVGGALVDISPEEAAERRVTGSVEFDSRAVGPGGLFLALPGARSDGHDHAASAIAAGAVAVLAARPVGV
PAIVVAPDPRTGDGGLTGVLEHDADGSGAAVLAALAKLAKAVAAELVAGGLTIIGITGSSGKTSTKDLVAVVLELLGEVV
APPESFNNELGHPWTVLRATRSTDYLILEMSARRPGNIAALAAIAPPKIGVVLNVGTAHLGEFGSREAIARTKTELPQAI
MQSGVVILNVDDPAVAAMADATVARVIRVSRGSYSHPGSPNSPDVWTGPVSLDELARPRFTLHTRDPRAGATEIQLGVYG
DHQVANALCAAAVGLECGASVEEVAVALAATGPVSRHRMQVTTRADGVTVIDDTYNANPDSMRAGLQALAWIAHGGTHDK
NQPGSCARRSWAVLGEMAELGEDSITEHDRIGRLAVRLDVSRLVVVGGGRSINAMHRGAVMEGSWGLETVNVADPAAALT
LLRAEVRPGDVVLIKASHSVGLGALADVLVGDGAIRP
>Mature_517_residues
MIDLTVARVTEIVGGALVDISPEEAAERRVTGSVEFDSRAVGPGGLFLALPGARSDGHDHAASAIAAGAVAVLAARPVGV
PAIVVAPDPRTGDGGLTGVLEHDADGSGAAVLAALAKLAKAVAAELVAGGLTIIGITGSSGKTSTKDLVAVVLELLGEVV
APPESFNNELGHPWTVLRATRSTDYLILEMSARRPGNIAALAAIAPPKIGVVLNVGTAHLGEFGSREAIARTKTELPQAI
MQSGVVILNVDDPAVAAMADATVARVIRVSRGSYSHPGSPNSPDVWTGPVSLDELARPRFTLHTRDPRAGATEIQLGVYG
DHQVANALCAAAVGLECGASVEEVAVALAATGPVSRHRMQVTTRADGVTVIDDTYNANPDSMRAGLQALAWIAHGGTHDK
NQPGSCARRSWAVLGEMAELGEDSITEHDRIGRLAVRLDVSRLVVVGGGRSINAMHRGAVMEGSWGLETVNVADPAAALT
LLRAEVRPGDVVLIKASHSVGLGALADVLVGDGAIRP

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786274, Length=451, Percent_Identity=31.9290465631929, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURF_MYCLE (O69556)

Other databases:

- EMBL:   AL022602
- EMBL:   AL583920
- PIR:   H87022
- RefSeq:   NP_301693.1
- ProteinModelPortal:   O69556
- SMR:   O69556
- EnsemblBacteria:   EBMYCT00000029340
- GeneID:   909915
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0910
- NMPDR:   fig|272631.1.peg.565
- Leproma:   ML0910
- GeneTree:   EBGT00050000015779
- HOGENOM:   HBG617121
- OMA:   ANPASMR
- ProtClustDB:   CLSK791663
- BioCyc:   MLEP272631:ML0910-MONOMER
- BRENDA:   6.3.2.10
- GO:   GO:0005737
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- PANTHER:   PTHR23135:SF3
- TIGRFAMs:   TIGR01143

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.10

Molecular weight: Translated: 52875; Mature: 52875

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLTVARVTEIVGGALVDISPEEAAERRVTGSVEFDSRAVGPGGLFLALPGARSDGHDH
CCCCHHHHHHHHHCCEEEECCHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCCCCCHH
AASAIAAGAVAVLAARPVGVPAIVVAPDPRTGDGGLTGVLEHDADGSGAAVLAALAKLAK
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AVAAELVAGGLTIIGITGSSGKTSTKDLVAVVLELLGEVVAPPESFNNELGHPWTVLRAT
HHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEC
RSTDYLILEMSARRPGNIAALAAIAPPKIGVVLNVGTAHLGEFGSREAIARTKTELPQAI
CCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHHHHH
MQSGVVILNVDDPAVAAMADATVARVIRVSRGSYSHPGSPNSPDVWTGPVSLDELARPRF
HHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCHHHHCCCCE
TLHTRDPRAGATEIQLGVYGDHQVANALCAAAVGLECGASVEEVAVALAATGPVSRHRMQ
EEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEE
VTTRADGVTVIDDTYNANPDSMRAGLQALAWIAHGGTHDKNQPGSCARRSWAVLGEMAEL
EEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHC
GEDSITEHDRIGRLAVRLDVSRLVVVGGGRSINAMHRGAVMEGSWGLETVNVADPAAALT
CCCCCCCHHHHCEEEEEEEEEEEEEEECCCEECHHHCCEEEECCCCCEEEECCCHHHHHH
LLRAEVRPGDVVLIKASHSVGLGALADVLVGDGAIRP
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIDLTVARVTEIVGGALVDISPEEAAERRVTGSVEFDSRAVGPGGLFLALPGARSDGHDH
CCCCHHHHHHHHHCCEEEECCHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCCCCCHH
AASAIAAGAVAVLAARPVGVPAIVVAPDPRTGDGGLTGVLEHDADGSGAAVLAALAKLAK
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AVAAELVAGGLTIIGITGSSGKTSTKDLVAVVLELLGEVVAPPESFNNELGHPWTVLRAT
HHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEC
RSTDYLILEMSARRPGNIAALAAIAPPKIGVVLNVGTAHLGEFGSREAIARTKTELPQAI
CCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHHHHH
MQSGVVILNVDDPAVAAMADATVARVIRVSRGSYSHPGSPNSPDVWTGPVSLDELARPRF
HHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCHHHHCCCCE
TLHTRDPRAGATEIQLGVYGDHQVANALCAAAVGLECGASVEEVAVALAATGPVSRHRMQ
EEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEE
VTTRADGVTVIDDTYNANPDSMRAGLQALAWIAHGGTHDKNQPGSCARRSWAVLGEMAEL
EEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHC
GEDSITEHDRIGRLAVRLDVSRLVVVGGGRSINAMHRGAVMEGSWGLETVNVADPAAALT
CCCCCCCHHHHCEEEEEEEEEEEEEEECCCEECHHHCCEEEECCCCCEEEECCCHHHHHH
LLRAEVRPGDVVLIKASHSVGLGALADVLVGDGAIRP
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002