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The map label for this gene is lipB

Identifier: 221229861

GI number: 221229861

Start: 1021453

End: 1022160

Strand: Reverse

Name: lipB

Synonym: MLBr_00859

Alternate gene names: 221229861

Gene position: 1022160-1021453 (Counterclockwise)

Preceding gene: 221229862

Following gene: 221229860

Centisome position: 31.28

GC content: 64.12

Gene sequence:

>708_bases
ATGGATTCCATCCGGTCGATCCCGACCGCGATCGATGTCCGCCAGCTAGGCACCGTCGACTACCACATCGCCTGGCAACT
ACAGCGAGACCTGGCCGACGCCAGGGTCGCCGGCGGACCCGACACGCTGCTGCTACTGCAGCACCCGCCAGTCTACACCG
CCGGACGGCGAACTCAGCCACACGAACGACCAAACCCGTCGCTGCACGGCGTTCCCGTTGTGGAAACCGACCGCGGAGGC
AAGATCACCTGGCATGGCCCAGGGCAGTTGGTCGGCTACCCGATCATTGGGCTTGCCGAGCCGCTCGACGTAGTCAACTA
TGTTCGACGCCTCGAGGAAGCATTGATCAAGGTCTGTGCGGAACTGGGCCTGGACACCAGCCGAGTCAACGGCCGCTCCG
GGATCTGGGTGCCGGGCAGTGCCGGTCAGCCTGCGCGCAAAATCGCCGCTATCGGCGTCCGGGTGTCACGTGCGACGACG
ATGCACGGATTCGCGCTTAATTGCCAGTGCGATCTGGACGCCTTCCACGCCATCGTACCTTGCGGCATCAGCGACGCCGG
GGTGACATCGTTGTCCGCCGAACTCGGACGCACCGTTGCCGTCAACGATGTCCGCTCAGCGATCGCCGAGGCCGTCAACG
ACGCTTTGGACGGTTGGTTAGCCCTGAGCTGGCTGTCCACTCCTGCCAGCGTAACATCGACACTGTGA

Upstream 100 bases:

>100_bases
CTCAGTTACGCGACCACCCGACCGAGCCAGAACTAGGCTCGGGCCGCAACACACCCGACGACCCAAACACCCACAGGCTA
CGGGTAGCCTGGGCTCCGTG

Downstream 100 bases:

>100_bases
CTGTTGCGCCCGAAGACTGCCGCTTACTGCGATTAGAAGTGCGCAACGCACAGACCCCGATCGAGCGCAAGCCGCCATGG
ATCAAAACCCGGGCCCGCAT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MDSIRSIPTAIDVRQLGTVDYHIAWQLQRDLADARVAGGPDTLLLLQHPPVYTAGRRTQPHERPNPSLHGVPVVETDRGG
KITWHGPGQLVGYPIIGLAEPLDVVNYVRRLEEALIKVCAELGLDTSRVNGRSGIWVPGSAGQPARKIAAIGVRVSRATT
MHGFALNCQCDLDAFHAIVPCGISDAGVTSLSAELGRTVAVNDVRSAIAEAVNDALDGWLALSWLSTPASVTSTL

Sequences:

>Translated_235_residues
MDSIRSIPTAIDVRQLGTVDYHIAWQLQRDLADARVAGGPDTLLLLQHPPVYTAGRRTQPHERPNPSLHGVPVVETDRGG
KITWHGPGQLVGYPIIGLAEPLDVVNYVRRLEEALIKVCAELGLDTSRVNGRSGIWVPGSAGQPARKIAAIGVRVSRATT
MHGFALNCQCDLDAFHAIVPCGISDAGVTSLSAELGRTVAVNDVRSAIAEAVNDALDGWLALSWLSTPASVTSTL
>Mature_235_residues
MDSIRSIPTAIDVRQLGTVDYHIAWQLQRDLADARVAGGPDTLLLLQHPPVYTAGRRTQPHERPNPSLHGVPVVETDRGG
KITWHGPGQLVGYPIIGLAEPLDVVNYVRRLEEALIKVCAELGLDTSRVNGRSGIWVPGSAGQPARKIAAIGVRVSRATT
MHGFALNCQCDLDAFHAIVPCGISDAGVTSLSAELGRTVAVNDVRSAIAEAVNDALDGWLALSWLSTPASVTSTL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=178, Percent_Identity=41.5730337078652, Blast_Score=103, Evalue=9e-23,
Organism=Escherichia coli, GI87081767, Length=183, Percent_Identity=33.879781420765, Blast_Score=104, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21355483, Length=216, Percent_Identity=37.5, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_MYCLB (B8ZQK0)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503277.1
- ProteinModelPortal:   B8ZQK0
- SMR:   B8ZQK0
- EnsemblBacteria:   EBMYCT00000085707
- GeneID:   7325387
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016282
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- ProtClustDB:   PRK14345
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25003; Mature: 25003

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSIRSIPTAIDVRQLGTVDYHIAWQLQRDLADARVAGGPDTLLLLQHPPVYTAGRRTQP
CCCHHCCCCHHHHHHHCCEEEEEEEEEHHHHHHHCCCCCCCEEEEEECCCCEECCCCCCC
HERPNPSLHGVPVVETDRGGKITWHGPGQLVGYPIIGLAEPLDVVNYVRRLEEALIKVCA
CCCCCCCCCCCEEEEECCCCEEEECCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHHHH
ELGLDTSRVNGRSGIWVPGSAGQPARKIAAIGVRVSRATTMHGFALNCQCDLDAFHAIVP
HHCCCCHHCCCCCCEEECCCCCCHHHHHHHHHEEEEEEEEECCEEEEEEECHHHHHHEEC
CGISDAGVTSLSAELGRTVAVNDVRSAIAEAVNDALDGWLALSWLSTPASVTSTL
CCCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHEEEHHHCCCCHHHCCC
>Mature Secondary Structure
MDSIRSIPTAIDVRQLGTVDYHIAWQLQRDLADARVAGGPDTLLLLQHPPVYTAGRRTQP
CCCHHCCCCHHHHHHHCCEEEEEEEEEHHHHHHHCCCCCCCEEEEEECCCCEECCCCCCC
HERPNPSLHGVPVVETDRGGKITWHGPGQLVGYPIIGLAEPLDVVNYVRRLEEALIKVCA
CCCCCCCCCCCEEEEECCCCEEEECCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHHHH
ELGLDTSRVNGRSGIWVPGSAGQPARKIAAIGVRVSRATTMHGFALNCQCDLDAFHAIVP
HHCCCCHHCCCCCCEEECCCCCCHHHHHHHHHEEEEEEEEECCEEEEEEECHHHHHHEEC
CGISDAGVTSLSAELGRTVAVNDVRSAIAEAVNDALDGWLALSWLSTPASVTSTL
CCCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHEEEHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA