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The map label for this gene is lipA

Identifier: 221229860

GI number: 221229860

Start: 1020512

End: 1021456

Strand: Reverse

Name: lipA

Synonym: MLBr_00858

Alternate gene names: 221229860

Gene position: 1021456-1020512 (Counterclockwise)

Preceding gene: 221229861

Following gene: 221229859

Centisome position: 31.26

GC content: 63.17

Gene sequence:

>945_bases
GTGACTGTTGCGCCCGAAGACTGCCGCTTACTGCGATTAGAAGTGCGCAACGCACAGACCCCGATCGAGCGCAAGCCGCC
ATGGATCAAAACCCGGGCCCGCATGGGACCAGAGTACACCGCGCTAAAAAACCTGGTCCGCCGTGTTGCTCTGCACACGG
TCTGCGAAGAGGCCGGTTGCCCCAACATCTTCGAATGCTGGGAAGATCGGGAAGCCACCTTTCTGATCGGTGGTGATCAG
TGCACCCGCCGGTGCGACTTCTGCCAGATCGACACCGGGAAGCCGGCTGCGTTGGACCGCGACGAGCCCCGACGGGTCGC
CGAGAGCGTGCAAACCATGGGGCTGCGGTATACCACCGTCACTGGGGTCGCCCGCGACGACTTGCCCGACGGCGGTGCCT
GGCTGTACGCCACAACCGTGCGAGCCATTAAAGAGCTCAATCCGTCGACCGGGGTCGAACTGTTGATTCCGGACTTCAAC
GGGCAGCCCGCGCGACTGGCGGAAGTCTTCGACTCACGCCCACAAGTGTTGGCGCACAACGTCGAAACCGTGCCACGCAT
CTTTAAACGGATTCGGCCTGCTTTCACCTACCAACGCAGCCTGGACGTGCTCACTGCAGCGCGCGAGGCCGGCCTGGTCA
CCAAAAGCAACCTGATCCTCGGCCTAGGCGAAACCGCTGATGAGGTGCGCACTGCCCTGGCCGATTTGCGCAAAACCGGC
TGCGACATCGTCACCATCACCCAATATCTGCGTCCGTCGATGCGCCACCACCCGGTCGAGCGCTGGGTCAGGCCTGAGGA
GTTCGTCGAGTACACGCAATACGCCGAAGGTTTGGGCTTCTCGGGTGTGCTGGGCGGGCCGCTGGTGCGGTCATCGTATC
GCGCCGGTCGGCTCTACGAGCAGGCTGCAGGCACCCGGACCGTCGGTACCTCGGTATCCCGTTAA

Upstream 100 bases:

>100_bases
ACGATGTCCGCTCAGCGATCGCCGAGGCCGTCAACGACGCTTTGGACGGTTGGTTAGCCCTGAGCTGGCTGTCCACTCCT
GCCAGCGTAACATCGACACT

Downstream 100 bases:

>100_bases
GCCGCACCTACGTATTCTTGATAACTATGGCTAAACCCCGAAATGCCGCTGCGCACAAGGCCGCGAGAGCGGAGGCGAAA
GCTGCCCGTAAGGCCGCGTC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTVAPEDCRLLRLEVRNAQTPIERKPPWIKTRARMGPEYTALKNLVRRVALHTVCEEAGCPNIFECWEDREATFLIGGDQ
CTRRCDFCQIDTGKPAALDRDEPRRVAESVQTMGLRYTTVTGVARDDLPDGGAWLYATTVRAIKELNPSTGVELLIPDFN
GQPARLAEVFDSRPQVLAHNVETVPRIFKRIRPAFTYQRSLDVLTAAREAGLVTKSNLILGLGETADEVRTALADLRKTG
CDIVTITQYLRPSMRHHPVERWVRPEEFVEYTQYAEGLGFSGVLGGPLVRSSYRAGRLYEQAAGTRTVGTSVSR

Sequences:

>Translated_314_residues
MTVAPEDCRLLRLEVRNAQTPIERKPPWIKTRARMGPEYTALKNLVRRVALHTVCEEAGCPNIFECWEDREATFLIGGDQ
CTRRCDFCQIDTGKPAALDRDEPRRVAESVQTMGLRYTTVTGVARDDLPDGGAWLYATTVRAIKELNPSTGVELLIPDFN
GQPARLAEVFDSRPQVLAHNVETVPRIFKRIRPAFTYQRSLDVLTAAREAGLVTKSNLILGLGETADEVRTALADLRKTG
CDIVTITQYLRPSMRHHPVERWVRPEEFVEYTQYAEGLGFSGVLGGPLVRSSYRAGRLYEQAAGTRTVGTSVSR
>Mature_313_residues
TVAPEDCRLLRLEVRNAQTPIERKPPWIKTRARMGPEYTALKNLVRRVALHTVCEEAGCPNIFECWEDREATFLIGGDQC
TRRCDFCQIDTGKPAALDRDEPRRVAESVQTMGLRYTTVTGVARDDLPDGGAWLYATTVRAIKELNPSTGVELLIPDFNG
QPARLAEVFDSRPQVLAHNVETVPRIFKRIRPAFTYQRSLDVLTAAREAGLVTKSNLILGLGETADEVRTALADLRKTGC
DIVTITQYLRPSMRHHPVERWVRPEEFVEYTQYAEGLGFSGVLGGPLVRSSYRAGRLYEQAAGTRTVGTSVSR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=43.859649122807, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=43.9024390243902, Blast_Score=200, Evalue=1e-51,
Organism=Escherichia coli, GI1786846, Length=294, Percent_Identity=42.5170068027211, Blast_Score=244, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI32564533, Length=291, Percent_Identity=41.9243986254296, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=42.6573426573427, Blast_Score=216, Evalue=3e-57,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=44.7552447552448, Blast_Score=234, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_MYCLB (B8ZQJ9)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503276.1
- ProteinModelPortal:   B8ZQJ9
- EnsemblBacteria:   EBMYCT00000086757
- GeneID:   7325073
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017436
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35120; Mature: 34989

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAPEDCRLLRLEVRNAQTPIERKPPWIKTRARMGPEYTALKNLVRRVALHTVCEEAGC
CCCCCCHHHEEEEEHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PNIFECWEDREATFLIGGDQCTRRCDFCQIDTGKPAALDRDEPRRVAESVQTMGLRYTTV
CCHHHHHCCCCEEEEECCHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEE
TGVARDDLPDGGAWLYATTVRAIKELNPSTGVELLIPDFNGQPARLAEVFDSRPQVLAHN
ECCHHCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHC
VETVPRIFKRIRPAFTYQRSLDVLTAAREAGLVTKSNLILGLGETADEVRTALADLRKTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCHHHHHHHHHHHHHCC
CDIVTITQYLRPSMRHHPVERWVRPEEFVEYTQYAEGLGFSGVLGGPLVRSSYRAGRLYE
CCEEEHHHHHCHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCHHHHHHHH
QAAGTRTVGTSVSR
HHCCCCCCCCCCCC
>Mature Secondary Structure 
TVAPEDCRLLRLEVRNAQTPIERKPPWIKTRARMGPEYTALKNLVRRVALHTVCEEAGC
CCCCCHHHEEEEEHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PNIFECWEDREATFLIGGDQCTRRCDFCQIDTGKPAALDRDEPRRVAESVQTMGLRYTTV
CCHHHHHCCCCEEEEECCHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEE
TGVARDDLPDGGAWLYATTVRAIKELNPSTGVELLIPDFNGQPARLAEVFDSRPQVLAHN
ECCHHCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHC
VETVPRIFKRIRPAFTYQRSLDVLTAAREAGLVTKSNLILGLGETADEVRTALADLRKTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCHHHHHHHHHHHHHCC
CDIVTITQYLRPSMRHHPVERWVRPEEFVEYTQYAEGLGFSGVLGGPLVRSSYRAGRLYE
CCEEEHHHHHCHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCHHHHHHHH
QAAGTRTVGTSVSR
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA