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The map label for this gene is pgk

Identifier: 221229698

GI number: 221229698

Start: 695920

End: 697170

Strand: Direct

Name: pgk

Synonym: MLBr_00571

Alternate gene names: 221229698

Gene position: 695920-697170 (Clockwise)

Preceding gene: 221229697

Following gene: 221229699

Centisome position: 21.29

GC content: 59.15

Gene sequence:

>1251_bases
GTGCGCATTCCTAATCTTAAAGATCTTCTTGAGGAAGGTGTTTCTGGTCGCTGCGTGTTGGTGCGCTGCGATCTGAACGT
CCCGCTGGGGGATGATGGCGCCATCACCGACCTCGGTCGGGTCACCGCGTCGGTGCCTACGTTGAAAGCGCTGCTTGAGG
CCGGAGCCAAGATTGTGGTTGCCGCCCATCTGGGCCGCCCCAAGAACGGGCCAGACCCAAAGCTGTCACTGGAGCCGGTC
GCCGCGGCGCTCGGTGAGCAGCTGGGCCAAAACGTCCAGTTGGTCTGCAGCACCGATCGTTCCCCTGTCGGGGGTGATGC
GCTCGCCTGTGTTGAGAGGCTGACCGACGGCGACCTCCTACTGCTGCAGAACATCCGCTTCGATCCGCGCGAGACCAGCA
AAGTCGACGACGAACGACTCGCTCTGGCCAAGCAATTGGTTGAATTGGTCGGGTCAGCAGGAGCTTTTGTTTCGGATGGA
TTCGGTGTGGTGCATCGCAGGCAGGCTTCGGTGTATGACGTCGCTACGCTATTGCCGCACTACGCCGGCATACTGGTTGC
TGACGAGATAAGGATACTCGAGCAATTGACTAGTTCGGCCAAACGCCCCTACGCAGTGGTGCTCGGCGGGTCGAAGGTAT
CCGACAAGCTCGGCGTCATCGAGTCGCTGGCGACAAAAGCCGATAGCATCGTACTCGGAGGTGGAATGTGCTTTACATTT
CTTGCTGCACAAGGATTTTCAGTAGGTAAATCGTTATTGGAAACCGAGATGATCGAGACCTGTCGTAGTCTGTTGGACAC
CTACGCTGACGTGCTTCGGCTTCCTATGGACATTGTGGTGACAGAGAAGTTCGTCGCTGATTCGCCGCCGCAGACCGTCG
CCGCCGACGCTATCCCAGATGGATTGATGGGACTGGATATCGGGCCGGAATCGGTCAAGCGGTTCGCTACGTTGTTATCC
AACGCCTCGACCATTTTCTGGAACGGACCGATGGGCGTGTTCGAATTCCCTGCGTACGCCGCGGGCACCAGAGGTGTTGC
TGAAGCAATCGTGGCTGTTACTGGCAAGGGAGCTTTCAGCGTGGTCGGCGGCGGTGACTCTGCGGCTGCGATGCGGGCGC
TCAGCCTACCCGAAGGCAGTGTCTCGCACCTTTCCACCGGTGGCGGCGCGTCGTTGGAATACCTTGAGGGCAAGACGCTA
CCCGGGATCGAGGTACTAGGTCGTGAGCAGCCAAGGGGAGGAGACTCGTGA

Upstream 100 bases:

>100_bases
TCGCCAGCCAGGCCAAGGTGGTGTCCTGGTACGATAACGAGTGGGGCTACTCCAACCGTCTGGTTGATCTGGTCGGCCTG
GTTGGCAAGTCACTTTAGCC

Downstream 100 bases:

>100_bases
GCCGCAAGTCGCTCATCGCCGGCAACTGGAAGATGAACCTGAATCATTTCGAGGCGATTGCGTTGGTGCAAAAAATCGCT
TTTTCATTGCCGGACAAGTA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MRIPNLKDLLEEGVSGRCVLVRCDLNVPLGDDGAITDLGRVTASVPTLKALLEAGAKIVVAAHLGRPKNGPDPKLSLEPV
AAALGEQLGQNVQLVCSTDRSPVGGDALACVERLTDGDLLLLQNIRFDPRETSKVDDERLALAKQLVELVGSAGAFVSDG
FGVVHRRQASVYDVATLLPHYAGILVADEIRILEQLTSSAKRPYAVVLGGSKVSDKLGVIESLATKADSIVLGGGMCFTF
LAAQGFSVGKSLLETEMIETCRSLLDTYADVLRLPMDIVVTEKFVADSPPQTVAADAIPDGLMGLDIGPESVKRFATLLS
NASTIFWNGPMGVFEFPAYAAGTRGVAEAIVAVTGKGAFSVVGGGDSAAAMRALSLPEGSVSHLSTGGGASLEYLEGKTL
PGIEVLGREQPRGGDS

Sequences:

>Translated_416_residues
MRIPNLKDLLEEGVSGRCVLVRCDLNVPLGDDGAITDLGRVTASVPTLKALLEAGAKIVVAAHLGRPKNGPDPKLSLEPV
AAALGEQLGQNVQLVCSTDRSPVGGDALACVERLTDGDLLLLQNIRFDPRETSKVDDERLALAKQLVELVGSAGAFVSDG
FGVVHRRQASVYDVATLLPHYAGILVADEIRILEQLTSSAKRPYAVVLGGSKVSDKLGVIESLATKADSIVLGGGMCFTF
LAAQGFSVGKSLLETEMIETCRSLLDTYADVLRLPMDIVVTEKFVADSPPQTVAADAIPDGLMGLDIGPESVKRFATLLS
NASTIFWNGPMGVFEFPAYAAGTRGVAEAIVAVTGKGAFSVVGGGDSAAAMRALSLPEGSVSHLSTGGGASLEYLEGKTL
PGIEVLGREQPRGGDS
>Mature_416_residues
MRIPNLKDLLEEGVSGRCVLVRCDLNVPLGDDGAITDLGRVTASVPTLKALLEAGAKIVVAAHLGRPKNGPDPKLSLEPV
AAALGEQLGQNVQLVCSTDRSPVGGDALACVERLTDGDLLLLQNIRFDPRETSKVDDERLALAKQLVELVGSAGAFVSDG
FGVVHRRQASVYDVATLLPHYAGILVADEIRILEQLTSSAKRPYAVVLGGSKVSDKLGVIESLATKADSIVLGGGMCFTF
LAAQGFSVGKSLLETEMIETCRSLLDTYADVLRLPMDIVVTEKFVADSPPQTVAADAIPDGLMGLDIGPESVKRFATLLS
NASTIFWNGPMGVFEFPAYAAGTRGVAEAIVAVTGKGAFSVVGGGDSAAAMRALSLPEGSVSHLSTGGGASLEYLEGKTL
PGIEVLGREQPRGGDS

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=422, Percent_Identity=37.2037914691943, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI31543397, Length=409, Percent_Identity=37.1638141809291, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI1789294, Length=408, Percent_Identity=39.4607843137255, Blast_Score=253, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17508823, Length=411, Percent_Identity=38.9294403892944, Blast_Score=272, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6319857, Length=409, Percent_Identity=38.1418092909535, Blast_Score=253, Evalue=4e-68,
Organism=Drosophila melanogaster, GI17136394, Length=414, Percent_Identity=37.1980676328502, Blast_Score=247, Evalue=9e-66,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=36.6265060240964, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_MYCLB (B8ZUP2)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503114.1
- ProteinModelPortal:   B8ZUP2
- SMR:   B8ZUP2
- EnsemblBacteria:   EBMYCT00000087438
- GeneID:   7325330
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017271
- HOGENOM:   HBG453500
- OMA:   STYGIAQ
- ProtClustDB:   PRK00073
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43279; Mature: 43279

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 40-40 BINDING 126-126 BINDING 166-166 BINDING 216-216 BINDING 304-304 BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPNLKDLLEEGVSGRCVLVRCDLNVPLGDDGAITDLGRVTASVPTLKALLEAGAKIVV
CCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEE
AAHLGRPKNGPDPKLSLEPVAAALGEQLGQNVQLVCSTDRSPVGGDALACVERLTDGDLL
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEE
LLQNIRFDPRETSKVDDERLALAKQLVELVGSAGAFVSDGFGVVHRRQASVYDVATLLPH
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHCCCHHHHHHHHHHH
YAGILVADEIRILEQLTSSAKRPYAVVLGGSKVSDKLGVIESLATKADSIVLGGGMCFTF
HHCEEHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHHHH
LAAQGFSVGKSLLETEMIETCRSLLDTYADVLRLPMDIVVTEKFVADSPPQTVAADAIPD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHCCCCCCCEEHHCCCC
GLMGLDIGPESVKRFATLLSNASTIFWNGPMGVFEFPAYAAGTRGVAEAIVAVTGKGAFS
CCEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHCCCCHHCCCHHHHHHHHHEECCCEEE
VVGGGDSAAAMRALSLPEGSVSHLSTGGGASLEYLEGKTLPGIEVLGREQPRGGDS
EECCCCHHHHHHHHCCCCCCHHHCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MRIPNLKDLLEEGVSGRCVLVRCDLNVPLGDDGAITDLGRVTASVPTLKALLEAGAKIVV
CCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEE
AAHLGRPKNGPDPKLSLEPVAAALGEQLGQNVQLVCSTDRSPVGGDALACVERLTDGDLL
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEE
LLQNIRFDPRETSKVDDERLALAKQLVELVGSAGAFVSDGFGVVHRRQASVYDVATLLPH
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHCCCHHHHHHHHHHH
YAGILVADEIRILEQLTSSAKRPYAVVLGGSKVSDKLGVIESLATKADSIVLGGGMCFTF
HHCEEHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHHHH
LAAQGFSVGKSLLETEMIETCRSLLDTYADVLRLPMDIVVTEKFVADSPPQTVAADAIPD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHCCCCCCCEEHHCCCC
GLMGLDIGPESVKRFATLLSNASTIFWNGPMGVFEFPAYAAGTRGVAEAIVAVTGKGAFS
CCEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHCCCCHHCCCHHHHHHHHHEECCCEEE
VVGGGDSAAAMRALSLPEGSVSHLSTGGGASLEYLEGKTLPGIEVLGREQPRGGDS
EECCCCHHHHHHHHCCCCCCHHHCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA