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The map label for this gene is tpiA

Identifier: 221229699

GI number: 221229699

Start: 697167

End: 697952

Strand: Direct

Name: tpiA

Synonym: MLBr_00572

Alternate gene names: 221229699

Gene position: 697167-697952 (Clockwise)

Preceding gene: 221229698

Following gene: 221229700

Centisome position: 21.33

GC content: 59.29

Gene sequence:

>786_bases
GTGAGCCGCAAGTCGCTCATCGCCGGCAACTGGAAGATGAACCTGAATCATTTCGAGGCGATTGCGTTGGTGCAAAAAAT
CGCTTTTTCATTGCCGGACAAGTATTATGACAAGGTTGATGTCACAGTTCTTCCGCCATTCACTGACCTGCGTAGCGTGC
AAACCCTGGTCGATGGCGATAAGTTGCGATTGACCTATGGTGCCCAGGATCTTTCGCAACATGACCTAGGCGCCTACACT
GGAGACATCAGTGGGGCCTTCTTGGCTAAGTTGGGCTGTAGCTTCGTGCTGGTGGGGCACTCTGAGCGGCGCACCTACCA
CGACGAAGGTGACGCGCTGGTGGCCGCGAAGACGGCTGCGGCGCTCAAAAACAGCTTGACTCCGATCGTGTGCATCGGCG
AGTACCTCGAGATCCGAGAGGTGGGTGAACACGTCAGCCACTGCCAGAACCAGCTACGCGGCTCGCTGGCTGGGCTGTCG
CCTGAACAGATCGGCAATGTCGTGATCGTTTATGAACCGGTCTGGGCGATCGGGACTGGGCGGGTGGCTAGTGCAGCCGA
CGCCCAAGAGGTGTGCGAGGCGATCCGAAAAGAGCTGGGTGCGCTGGCATCACCGCAGGTGGCCGAAACCGTTCGGGTGC
TTTATGGTGGCTCGTTGAACGCCAAAAACATTGGCGATATCGTCGCCCAGCAAGACGTCGATGGTGGTCTGGTTGGGGGA
GCGTCGCTGGACGGGGCGCAATTCGCGACGCTCGCGGTCATCGCCGCCGGTGGGCCTCTTCCCTAG

Upstream 100 bases:

>100_bases
ACCTTTCCACCGGTGGCGGCGCGTCGTTGGAATACCTTGAGGGCAAGACGCTACCCGGGATCGAGGTACTAGGTCGTGAG
CAGCCAAGGGGAGGAGACTC

Downstream 100 bases:

>100_bases
GGAAGCCCTCGGGGCGTCTTGCACTGACTTGGTGATAGGTTATGCTGCAGACTTTAGCCGTAGCGTTAGCTCAGTGCTGT
GGGTGGCTAATTCGCTATGG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSRKSLIAGNWKMNLNHFEAIALVQKIAFSLPDKYYDKVDVTVLPPFTDLRSVQTLVDGDKLRLTYGAQDLSQHDLGAYT
GDISGAFLAKLGCSFVLVGHSERRTYHDEGDALVAAKTAAALKNSLTPIVCIGEYLEIREVGEHVSHCQNQLRGSLAGLS
PEQIGNVVIVYEPVWAIGTGRVASAADAQEVCEAIRKELGALASPQVAETVRVLYGGSLNAKNIGDIVAQQDVDGGLVGG
ASLDGAQFATLAVIAAGGPLP

Sequences:

>Translated_261_residues
MSRKSLIAGNWKMNLNHFEAIALVQKIAFSLPDKYYDKVDVTVLPPFTDLRSVQTLVDGDKLRLTYGAQDLSQHDLGAYT
GDISGAFLAKLGCSFVLVGHSERRTYHDEGDALVAAKTAAALKNSLTPIVCIGEYLEIREVGEHVSHCQNQLRGSLAGLS
PEQIGNVVIVYEPVWAIGTGRVASAADAQEVCEAIRKELGALASPQVAETVRVLYGGSLNAKNIGDIVAQQDVDGGLVGG
ASLDGAQFATLAVIAAGGPLP
>Mature_260_residues
SRKSLIAGNWKMNLNHFEAIALVQKIAFSLPDKYYDKVDVTVLPPFTDLRSVQTLVDGDKLRLTYGAQDLSQHDLGAYTG
DISGAFLAKLGCSFVLVGHSERRTYHDEGDALVAAKTAAALKNSLTPIVCIGEYLEIREVGEHVSHCQNQLRGSLAGLSP
EQIGNVVIVYEPVWAIGTGRVASAADAQEVCEAIRKELGALASPQVAETVRVLYGGSLNAKNIGDIVAQQDVDGGLVGGA
SLDGAQFATLAVIAAGGPLP

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=244, Percent_Identity=37.2950819672131, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI4507645, Length=244, Percent_Identity=37.2950819672131, Blast_Score=141, Evalue=5e-34,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=38.9558232931727, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17536593, Length=244, Percent_Identity=42.6229508196721, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320255, Length=244, Percent_Identity=38.9344262295082, Blast_Score=153, Evalue=3e-38,
Organism=Drosophila melanogaster, GI28572008, Length=244, Percent_Identity=39.7540983606557, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI28572006, Length=244, Percent_Identity=39.7540983606557, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI28572004, Length=244, Percent_Identity=39.7540983606557, Blast_Score=147, Evalue=5e-36,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_MYCLE (P46711)

Other databases:

- EMBL:   U00013
- EMBL:   AL583919
- PIR:   S72750
- RefSeq:   NP_301484.1
- ProteinModelPortal:   P46711
- EnsemblBacteria:   EBMYCT00000027948
- GeneID:   909354
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0572
- NMPDR:   fig|272631.1.peg.356
- Leproma:   ML0572
- GeneTree:   EBGT00050000016598
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   MLEP272631:ML0572-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27558; Mature: 27426

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 100-100 ACT_SITE 172-172 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKSLIAGNWKMNLNHFEAIALVQKIAFSLPDKYYDKVDVTVLPPFTDLRSVQTLVDGD
CCCCCEEECCEEECCHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCHHHHHHHHHHCCC
KLRLTYGAQDLSQHDLGAYTGDISGAFLAKLGCSFVLVGHSERRTYHDEGDALVAAKTAA
EEEEEECCCHHCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEHHHHH
ALKNSLTPIVCIGEYLEIREVGEHVSHCQNQLRGSLAGLSPEQIGNVVIVYEPVWAIGTG
HHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCEEECCC
RVASAADAQEVCEAIRKELGALASPQVAETVRVLYGGSLNAKNIGDIVAQQDVDGGLVGG
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEECC
ASLDGAQFATLAVIAAGGPLP
CCCCCHHHEEEEEEECCCCCC
>Mature Secondary Structure 
SRKSLIAGNWKMNLNHFEAIALVQKIAFSLPDKYYDKVDVTVLPPFTDLRSVQTLVDGD
CCCCEEECCEEECCHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCHHHHHHHHHHCCC
KLRLTYGAQDLSQHDLGAYTGDISGAFLAKLGCSFVLVGHSERRTYHDEGDALVAAKTAA
EEEEEECCCHHCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEHHHHH
ALKNSLTPIVCIGEYLEIREVGEHVSHCQNQLRGSLAGLSPEQIGNVVIVYEPVWAIGTG
HHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCEEECCC
RVASAADAQEVCEAIRKELGALASPQVAETVRVLYGGSLNAKNIGDIVAQQDVDGGLVGG
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEECC
ASLDGAQFATLAVIAAGGPLP
CCCCCHHHEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002