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The map label for this gene is gap

Identifier: 221229697

GI number: 221229697

Start: 694898

End: 695917

Strand: Direct

Name: gap

Synonym: MLBr_00570

Alternate gene names: 221229697

Gene position: 694898-695917 (Clockwise)

Preceding gene: 221229695

Following gene: 221229698

Centisome position: 21.26

GC content: 58.63

Gene sequence:

>1020_bases
GTGACGGTCCGCGTGGGCATTAACGGCTTCGGCCGCATCGGACGCAACTTCTACCGGGCGTTATTGGCCCAACAGGAACA
TGGTATTGCCGACGTGCAAGTAGTCGCCATCAACGACATCACCGACAACAGCACGCTGGCGTACCTGCTCAAATTCGACT
CCATCCTGGGCCGGCTGCCACACGATGTTAGCCTCGAGGAAGAGGATACAATCGTGGTTGGCTCCGAGAAAATCAAGGCT
CTTGCGGTTCGAGAAGGTCCAGCGGCATTGCCGTGGCATGCCTTCGGCGTCGACGTTGTCGTCGAGTCGACCGGCTTGTT
CACCAACGCGGCCAAGGCCAAAGGGCACTTGGAGGCCGGCGCCAAGAAAGTGATCGTTTCGGCACCTGCCACTGATCCCG
ATATCACCATCGTGTTCGGGGTCAACGACGACAAGTACGACGGCAGCCAGAACATCATCTCCAATGCGTCGTGTACGACG
AATTGCCTTGCACCGCTGGCGAAAGTGCTGCACGACCAATTCGGCATCGTTAAGGGTTTGATGACCACCGTCCACGCGTA
CACTCAAGACCAGAACCTGCAGGATGGACCGCATTCTGACCTGCGTCGTGCCCGCGCTGCCGCGCTGAACGTTGTGCCGA
CCTCTACCGGTGCGGCCAAGGCGATCGGCCTGGTGATGCCTGAGCTTAAGGGTAAGCTTGACGGTTACGCACTGCGGGTG
CCGATCCCTACCGGCTCGGTTACCGACCTGACAGCCGACTTGTCCAAGTGCGTCAGTGTCAATGAGATCAACGCGGTGTT
TCAGGACGCCGCTGAGGGTCGGCTTAAAGGCATCCTGAAGTACGTTGATGCCCCGATCGTCTCTAGCGACATCGTCACCG
ACCCGCACAGTTCGATCTTCGACTCCGGCCTTACCAAAGTTATCGCCAGCCAGGCCAAGGTGGTGTCCTGGTACGATAAC
GAGTGGGGCTACTCCAACCGTCTGGTTGATCTGGTCGGCCTGGTTGGCAAGTCACTTTAG

Upstream 100 bases:

>100_bases
GTGACATGCTCAACCGGTCCATGACTTCGGCTACCTGTCATTGATCTAGGGTGACGGCGAGCAGTTCATTAGCCGATTGA
AGACGACAAGGGAGAGACCA

Downstream 100 bases:

>100_bases
CCGTGCGCATTCCTAATCTTAAAGATCTTCTTGAGGAAGGTGTTTCTGGTCGCTGCGTGTTGGTGCGCTGCGATCTGAAC
GTCCCGCTGGGGGATGATGG

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTVRVGINGFGRIGRNFYRALLAQQEHGIADVQVVAINDITDNSTLAYLLKFDSILGRLPHDVSLEEEDTIVVGSEKIKA
LAVREGPAALPWHAFGVDVVVESTGLFTNAAKAKGHLEAGAKKVIVSAPATDPDITIVFGVNDDKYDGSQNIISNASCTT
NCLAPLAKVLHDQFGIVKGLMTTVHAYTQDQNLQDGPHSDLRRARAAALNVVPTSTGAAKAIGLVMPELKGKLDGYALRV
PIPTGSVTDLTADLSKCVSVNEINAVFQDAAEGRLKGILKYVDAPIVSSDIVTDPHSSIFDSGLTKVIASQAKVVSWYDN
EWGYSNRLVDLVGLVGKSL

Sequences:

>Translated_339_residues
MTVRVGINGFGRIGRNFYRALLAQQEHGIADVQVVAINDITDNSTLAYLLKFDSILGRLPHDVSLEEEDTIVVGSEKIKA
LAVREGPAALPWHAFGVDVVVESTGLFTNAAKAKGHLEAGAKKVIVSAPATDPDITIVFGVNDDKYDGSQNIISNASCTT
NCLAPLAKVLHDQFGIVKGLMTTVHAYTQDQNLQDGPHSDLRRARAAALNVVPTSTGAAKAIGLVMPELKGKLDGYALRV
PIPTGSVTDLTADLSKCVSVNEINAVFQDAAEGRLKGILKYVDAPIVSSDIVTDPHSSIFDSGLTKVIASQAKVVSWYDN
EWGYSNRLVDLVGLVGKSL
>Mature_338_residues
TVRVGINGFGRIGRNFYRALLAQQEHGIADVQVVAINDITDNSTLAYLLKFDSILGRLPHDVSLEEEDTIVVGSEKIKAL
AVREGPAALPWHAFGVDVVVESTGLFTNAAKAKGHLEAGAKKVIVSAPATDPDITIVFGVNDDKYDGSQNIISNASCTTN
CLAPLAKVLHDQFGIVKGLMTTVHAYTQDQNLQDGPHSDLRRARAAALNVVPTSTGAAKAIGLVMPELKGKLDGYALRVP
IPTGSVTDLTADLSKCVSVNEINAVFQDAAEGRLKGILKYVDAPIVSSDIVTDPHSSIFDSGLTKVIASQAKVVSWYDNE
WGYSNRLVDLVGLVGKSL

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=50.4504504504504, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI7657116, Length=332, Percent_Identity=48.4939759036145, Blast_Score=290, Evalue=9e-79,
Organism=Escherichia coli, GI1788079, Length=339, Percent_Identity=50.7374631268437, Blast_Score=320, Evalue=6e-89,
Organism=Escherichia coli, GI1789295, Length=332, Percent_Identity=43.0722891566265, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17534679, Length=339, Percent_Identity=52.212389380531, Blast_Score=317, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI17534677, Length=339, Percent_Identity=51.9174041297935, Blast_Score=316, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI32566163, Length=340, Percent_Identity=52.0588235294118, Blast_Score=310, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI17568413, Length=340, Percent_Identity=52.0588235294118, Blast_Score=310, Evalue=7e-85,
Organism=Saccharomyces cerevisiae, GI6321631, Length=339, Percent_Identity=50.4424778761062, Blast_Score=311, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6322468, Length=339, Percent_Identity=49.8525073746313, Blast_Score=310, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6322409, Length=339, Percent_Identity=48.0825958702065, Blast_Score=301, Evalue=9e-83,
Organism=Drosophila melanogaster, GI17933600, Length=332, Percent_Identity=50.9036144578313, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI18110149, Length=332, Percent_Identity=50.9036144578313, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI85725000, Length=332, Percent_Identity=50, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI22023983, Length=332, Percent_Identity=50, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI19922412, Length=331, Percent_Identity=47.7341389728097, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): G3P_MYCLE (P46713)

Other databases:

- EMBL:   U00013
- EMBL:   AL583919
- PIR:   S72763
- RefSeq:   NP_301482.1
- ProteinModelPortal:   P46713
- SMR:   P46713
- EnsemblBacteria:   EBMYCT00000029430
- GeneID:   909341
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0570
- NMPDR:   fig|272631.1.peg.354
- Leproma:   ML0570
- GeneTree:   EBGT00050000017630
- HOGENOM:   HBG571736
- OMA:   RNYFRAA
- ProtClustDB:   CLSK791163
- BioCyc:   MLEP272631:ML0570-MONOMER
- BRENDA:   1.2.1.12
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 36073; Mature: 35942

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 158-158 BINDING 39-39 BINDING 84-84 BINDING 188-188 BINDING 203-203 BINDING 239-239 BINDING 320-320

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRVGINGFGRIGRNFYRALLAQQEHGIADVQVVAINDITDNSTLAYLLKFDSILGRLP
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCC
HDVSLEEEDTIVVGSEKIKALAVREGPAALPWHAFGVDVVVESTGLFTNAAKAKGHLEAG
CCCCCCCCCEEEEECCCEEEEEEECCCCCCCCHHCCEEEEEECCCCEECCHHHCCHHHCC
AKKVIVSAPATDPDITIVFGVNDDKYDGSQNIISNASCTTNCLAPLAKVLHDQFGIVKGL
CEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MTTVHAYTQDQNLQDGPHSDLRRARAAALNVVPTSTGAAKAIGLVMPELKGKLDGYALRV
HHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEE
PIPTGSVTDLTADLSKCVSVNEINAVFQDAAEGRLKGILKYVDAPIVSSDIVTDPHSSIF
ECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
DSGLTKVIASQAKVVSWYDNEWGYSNRLVDLVGLVGKSL
HHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVRVGINGFGRIGRNFYRALLAQQEHGIADVQVVAINDITDNSTLAYLLKFDSILGRLP
EEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCC
HDVSLEEEDTIVVGSEKIKALAVREGPAALPWHAFGVDVVVESTGLFTNAAKAKGHLEAG
CCCCCCCCCEEEEECCCEEEEEEECCCCCCCCHHCCEEEEEECCCCEECCHHHCCHHHCC
AKKVIVSAPATDPDITIVFGVNDDKYDGSQNIISNASCTTNCLAPLAKVLHDQFGIVKGL
CEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MTTVHAYTQDQNLQDGPHSDLRRARAAALNVVPTSTGAAKAIGLVMPELKGKLDGYALRV
HHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEE
PIPTGSVTDLTADLSKCVSVNEINAVFQDAAEGRLKGILKYVDAPIVSSDIVTDPHSSIF
ECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
DSGLTKVIASQAKVVSWYDNEWGYSNRLVDLVGLVGKSL
HHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002