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The map label for this gene is aroF

Identifier: 221229656

GI number: 221229656

Start: 627750

End: 628973

Strand: Direct

Name: aroF

Synonym: MLBr_00516

Alternate gene names: 221229656

Gene position: 627750-628973 (Clockwise)

Preceding gene: 221229655

Following gene: 221229657

Centisome position: 19.21

GC content: 64.71

Gene sequence:

>1224_bases
GTGTTGCGCTGGATCACCGCCGGGGAGTCGCATGGCCGGGCTCTGGTGGCCGTTGTCGAAGGCATGGTCGCCGGTGTCGA
AGTTACCTCCACCGCCATCGCTGATCAGTTGGCGCGCCGCCGACTTGGCTATGGCCGCGGTGCCCGGATGGCCTTCGAGC
GCGACGGGGTGACGGTGTTGTCTGGGGTACGCCACGGCGTTACTTTGGGTGGCCCCATAGCCATCGAGATCGACAACACC
GAGTGGCCGAAGTGGGAATCCGTGATGGCCGCCGATCCGGTCGACGCCGCGGAATTGGAAGACTCCGCTCGCAACGCCCC
GCTCACCAGGCCGCGCCCGGGTCATGCGGACTATGCGGGTATGCTCAAGTACGGTTTCGACGACGCTCGTCCAGTGTTGG
AGCGGGCTAGTGCTCGAGAAACCGCCGCCCGTGTCGCGGCGGGGACGATCGCGAGGCAGTTTCTGCGCCAAGCTCTGGGG
GTCGAAGTGCTTTCGCACGTCATTTCCATCGGTGCGTCGGCCCGCTACGATGGGCCACCGCCGGGGTCCGAAGACCTAAC
CGTAATCGATGCTAGTCTGGTGCGCGCTTTTGACGAACAAGTTGAAAAATCTATGATCGCTGAGATTGAAGCGGCCAAGA
AAGACGGCGATACCCTCGGCGGGGTAGTAGAGGCCGTGGTGCTGGGCCTGCCAGTCGGGCTGGGGTCCTTTACCAGCGGT
GACGACCGGCTCGACAGCCAGCTAGCCGCAGCCGTAATGGGGATACAGGCGATTAAAGGCGTAGAGATCGGTGACGGCTT
CGCGACTGCGCGTCGCCGCGGCAGCTGCGCTCACGACGAAATGTATTCTGGTCCTGGCGGTGTCGTCCGCTTAACCAACC
GGTCAGGTGGACTCGAAGGCGGCATGACCAATGGCCAGCCGCTGCGGGTGCGCGCGGCGATGAAGCCGATCTCCACTGTG
CCGCGGGCGCTAGCCACCGTCGATCTGGCAACCGGTGAAGAGGCCATCGCAATCCACCAGCGCTCGGATGTGTGCGCGGT
GCCGGCCGCCGGAGTCGTTGTCGAGACCATGGTTGCGTTGGTGCTGGCCCGCGCAGCGCTGCGGAAGTTCGGCGGTGACT
CACTGGGTGAAACTCGTCGTAATCTTGCAGCGTACCAGCGCGCGGTCGCTGACCGGGAAGCGCCAGTTGCTCAGTACTGC
GGCGAGTCGGGGATAGGCGGGTAG

Upstream 100 bases:

>100_bases
GGCAGCATGGAAAAGTGCGAGAGCCCCCTACATGTTGGCGTCGATTTCGATGTTTGCGCGCATTTTGAGTGAACCCGAAA
TGGCATGGCAGGATAGTTAA

Downstream 100 bases:

>100_bases
GGGCGCGATGGCACCCAAGGCAGTACTCGTGGGTCTGCCAGGAGCCGGCAAGTCCACCATCGGGCGGCGACTGTCCAAGG
CGCTAGGGGTGAGCTTGCTC

Product: chorismate synthase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate phospholyase

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MLRWITAGESHGRALVAVVEGMVAGVEVTSTAIADQLARRRLGYGRGARMAFERDGVTVLSGVRHGVTLGGPIAIEIDNT
EWPKWESVMAADPVDAAELEDSARNAPLTRPRPGHADYAGMLKYGFDDARPVLERASARETAARVAAGTIARQFLRQALG
VEVLSHVISIGASARYDGPPPGSEDLTVIDASLVRAFDEQVEKSMIAEIEAAKKDGDTLGGVVEAVVLGLPVGLGSFTSG
DDRLDSQLAAAVMGIQAIKGVEIGDGFATARRRGSCAHDEMYSGPGGVVRLTNRSGGLEGGMTNGQPLRVRAAMKPISTV
PRALATVDLATGEEAIAIHQRSDVCAVPAAGVVVETMVALVLARAALRKFGGDSLGETRRNLAAYQRAVADREAPVAQYC
GESGIGG

Sequences:

>Translated_407_residues
MLRWITAGESHGRALVAVVEGMVAGVEVTSTAIADQLARRRLGYGRGARMAFERDGVTVLSGVRHGVTLGGPIAIEIDNT
EWPKWESVMAADPVDAAELEDSARNAPLTRPRPGHADYAGMLKYGFDDARPVLERASARETAARVAAGTIARQFLRQALG
VEVLSHVISIGASARYDGPPPGSEDLTVIDASLVRAFDEQVEKSMIAEIEAAKKDGDTLGGVVEAVVLGLPVGLGSFTSG
DDRLDSQLAAAVMGIQAIKGVEIGDGFATARRRGSCAHDEMYSGPGGVVRLTNRSGGLEGGMTNGQPLRVRAAMKPISTV
PRALATVDLATGEEAIAIHQRSDVCAVPAAGVVVETMVALVLARAALRKFGGDSLGETRRNLAAYQRAVADREAPVAQYC
GESGIGG
>Mature_407_residues
MLRWITAGESHGRALVAVVEGMVAGVEVTSTAIADQLARRRLGYGRGARMAFERDGVTVLSGVRHGVTLGGPIAIEIDNT
EWPKWESVMAADPVDAAELEDSARNAPLTRPRPGHADYAGMLKYGFDDARPVLERASARETAARVAAGTIARQFLRQALG
VEVLSHVISIGASARYDGPPPGSEDLTVIDASLVRAFDEQVEKSMIAEIEAAKKDGDTLGGVVEAVVLGLPVGLGSFTSG
DDRLDSQLAAAVMGIQAIKGVEIGDGFATARRRGSCAHDEMYSGPGGVVRLTNRSGGLEGGMTNGQPLRVRAAMKPISTV
PRALATVDLATGEEAIAIHQRSDVCAVPAAGVVVETMVALVLARAALRKFGGDSLGETRRNLAAYQRAVADREAPVAQYC
GESGIGG

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; seventh step. [C]

COG id: COG0082

COG function: function code E; Chorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chorismate synthase family

Homologues:

Organism=Escherichia coli, GI1788669, Length=369, Percent_Identity=34.9593495934959, Blast_Score=159, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6321290, Length=377, Percent_Identity=35.2785145888594, Blast_Score=178, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROC_MYCLB (B8ZUK0)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503072.1
- ProteinModelPortal:   B8ZUK0
- SMR:   B8ZUK0
- EnsemblBacteria:   EBMYCT00000085169
- GeneID:   7327233
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016560
- HOGENOM:   HBG292336
- OMA:   GSEAHDE
- ProtClustDB:   PRK05382
- HAMAP:   MF_00300_B
- InterPro:   IPR000453
- InterPro:   IPR020541
- PANTHER:   PTHR21085
- PIRSF:   PIRSF001456
- TIGRFAMs:   TIGR00033

Pfam domain/function: PF01264 Chorismate_synt; SSF103263 Chorismate_synth

EC number: =4.2.3.5

Molecular weight: Translated: 42474; Mature: 42474

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00787 CHORISMATE_SYNTHASE_1; PS00788 CHORISMATE_SYNTHASE_2; PS00789 CHORISMATE_SYNTHASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRWITAGESHGRALVAVVEGMVAGVEVTSTAIADQLARRRLGYGRGARMAFERDGVTVL
CCEEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHH
SGVRHGVTLGGPIAIEIDNTEWPKWESVMAADPVDAAELEDSARNAPLTRPRPGHADYAG
HHHHCCCEECCEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHH
MLKYGFDDARPVLERASARETAARVAAGTIARQFLRQALGVEVLSHVISIGASARYDGPP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PGSEDLTVIDASLVRAFDEQVEKSMIAEIEAAKKDGDTLGGVVEAVVLGLPVGLGSFTSG
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
DDRLDSQLAAAVMGIQAIKGVEIGDGFATARRRGSCAHDEMYSGPGGVVRLTNRSGGLEG
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCCC
GMTNGQPLRVRAAMKPISTVPRALATVDLATGEEAIAIHQRSDVCAVPAAGVVVETMVAL
CCCCCCCEEEHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCEECCCCHHHHHHHHHHH
VLARAALRKFGGDSLGETRRNLAAYQRAVADREAPVAQYCGESGIGG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MLRWITAGESHGRALVAVVEGMVAGVEVTSTAIADQLARRRLGYGRGARMAFERDGVTVL
CCEEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHH
SGVRHGVTLGGPIAIEIDNTEWPKWESVMAADPVDAAELEDSARNAPLTRPRPGHADYAG
HHHHCCCEECCEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHH
MLKYGFDDARPVLERASARETAARVAAGTIARQFLRQALGVEVLSHVISIGASARYDGPP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PGSEDLTVIDASLVRAFDEQVEKSMIAEIEAAKKDGDTLGGVVEAVVLGLPVGLGSFTSG
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
DDRLDSQLAAAVMGIQAIKGVEIGDGFATARRRGSCAHDEMYSGPGGVVRLTNRSGGLEG
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCCC
GMTNGQPLRVRAAMKPISTVPRALATVDLATGEEAIAIHQRSDVCAVPAAGVVVETMVAL
CCCCCCCEEEHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCEECCCCHHHHHHHHHHH
VLARAALRKFGGDSLGETRRNLAAYQRAVADREAPVAQYCGESGIGG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA