Click here to switch to the map view.

The map label for this gene is aroK

Identifier: 221229657

GI number: 221229657

Start: 628981

End: 629580

Strand: Direct

Name: aroK

Synonym: MLBr_00517

Alternate gene names: 221229657

Gene position: 628981-629580 (Clockwise)

Preceding gene: 221229656

Following gene: 221229658

Centisome position: 19.25

GC content: 60.5

Gene sequence:

>600_bases
ATGGCACCCAAGGCAGTACTCGTGGGTCTGCCAGGAGCCGGCAAGTCCACCATCGGGCGGCGACTGTCCAAGGCGCTAGG
GGTGAGCTTGCTCGATACCGACGCTGCTATTGAGAAGCAGACCGGTCGCAGCATCGCTGACATCTTTGCCATCGACGGGG
AAGAAGAGTTCCGCCGCATCGAAGAGGGCGTGGTGCGGGCTGCTTTAGTCGAGCATGACGGTGTGGTGTCGCTTGGCGGC
GGTGCTGTCACTAGCCCAGGGGTATGCGCGGCGCTCGCTGGCCACATTGTCATCTATCTGGAGATCAACGCCGAAGAAGC
AATGCGGCGTGCCTGTGGCAGTACCGTGCGACCGTTGCTGGCCGGGCCCGACCGTGCTGAAAAATTCCAGGATCTGATGG
CCAGACGGGTTCCTCTTTACCGGCGAGTTGCGACTATCCGGGTTGATACCAACTGCCACAATCTGGGGGCGGTAGTCCGC
TACATCATGGCCCGGTTGCAGGCTCAACTAGCCACTCCTGTCTCTGGTGGTGATCGCAAGAGTAGCGAAGCCGAGCGTAG
TGGGGCGCCACTTCGCAAATCTAGTGAGGTCGTCAAATGA

Upstream 100 bases:

>100_bases
ACTCGTCGTAATCTTGCAGCGTACCAGCGCGCGGTCGCTGACCGGGAAGCGCCAGTTGCTCAGTACTGCGGCGAGTCGGG
GATAGGCGGGTAGGGGCGCG

Downstream 100 bases:

>100_bases
CAAATATCGACGCACCGGTCACTGTGCAGGTGGCTGTCGACCCGCCATACCCAGTGGTGATCGGCACCGGGTTGTCGGAT
CAATTGGATGAACTGCTTGC

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MAPKAVLVGLPGAGKSTIGRRLSKALGVSLLDTDAAIEKQTGRSIADIFAIDGEEEFRRIEEGVVRAALVEHDGVVSLGG
GAVTSPGVCAALAGHIVIYLEINAEEAMRRACGSTVRPLLAGPDRAEKFQDLMARRVPLYRRVATIRVDTNCHNLGAVVR
YIMARLQAQLATPVSGGDRKSSEAERSGAPLRKSSEVVK

Sequences:

>Translated_199_residues
MAPKAVLVGLPGAGKSTIGRRLSKALGVSLLDTDAAIEKQTGRSIADIFAIDGEEEFRRIEEGVVRAALVEHDGVVSLGG
GAVTSPGVCAALAGHIVIYLEINAEEAMRRACGSTVRPLLAGPDRAEKFQDLMARRVPLYRRVATIRVDTNCHNLGAVVR
YIMARLQAQLATPVSGGDRKSSEAERSGAPLRKSSEVVK
>Mature_198_residues
APKAVLVGLPGAGKSTIGRRLSKALGVSLLDTDAAIEKQTGRSIADIFAIDGEEEFRRIEEGVVRAALVEHDGVVSLGGG
AVTSPGVCAALAGHIVIYLEINAEEAMRRACGSTVRPLLAGPDRAEKFQDLMARRVPLYRRVATIRVDTNCHNLGAVVRY
IMARLQAQLATPVSGGDRKSSEAERSGAPLRKSSEVVK

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=167, Percent_Identity=37.7245508982036, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_MYCLE (Q9CCS5)

Other databases:

- EMBL:   AL583918
- PIR:   E86973
- RefSeq:   NP_301442.1
- ProteinModelPortal:   Q9CCS5
- SMR:   Q9CCS5
- EnsemblBacteria:   EBMYCT00000029135
- GeneID:   909274
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0517
- NMPDR:   fig|272631.1.peg.314
- Leproma:   ML0517
- GeneTree:   EBGT00050000016251
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- ProtClustDB:   PRK00131
- BioCyc:   MLEP272631:ML0517-MONOMER
- BRENDA:   2.7.1.71
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 21085; Mature: 20953

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 34-34 BINDING 58-58 BINDING 80-80 BINDING 117-117 BINDING 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPKAVLVGLPGAGKSTIGRRLSKALGVSLLDTDAAIEKQTGRSIADIFAIDGEEEFRRI
CCCCEEEEECCCCCHHHHHHHHHHHHCCHHCCCHHHHHHHCCCHHHHHEEECCHHHHHHH
EEGVVRAALVEHDGVVSLGGGAVTSPGVCAALAGHIVIYLEINAEEAMRRACGSTVRPLL
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCEEEEEEECHHHHHHHHHCHHHHHHH
AGPDRAEKFQDLMARRVPLYRRVATIRVDTNCHNLGAVVRYIMARLQAQLATPVSGGDRK
CCCCHHHHHHHHHHHHCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SSEAERSGAPLRKSSEVVK
CCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
APKAVLVGLPGAGKSTIGRRLSKALGVSLLDTDAAIEKQTGRSIADIFAIDGEEEFRRI
CCCEEEEECCCCCHHHHHHHHHHHHCCHHCCCHHHHHHHCCCHHHHHEEECCHHHHHHH
EEGVVRAALVEHDGVVSLGGGAVTSPGVCAALAGHIVIYLEINAEEAMRRACGSTVRPLL
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCEEEEEEECHHHHHHHHHCHHHHHHH
AGPDRAEKFQDLMARRVPLYRRVATIRVDTNCHNLGAVVRYIMARLQAQLATPVSGGDRK
CCCCHHHHHHHHHHHHCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SSEAERSGAPLRKSSEVVK
CCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002