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The map label for this gene is guaA

Identifier: 221229598

GI number: 221229598

Start: 491052

End: 492641

Strand: Direct

Name: guaA

Synonym: MLBr_00395

Alternate gene names: 221229598

Gene position: 491052-492641 (Clockwise)

Preceding gene: 221229594

Following gene: 221229602

Centisome position: 15.03

GC content: 61.26

Gene sequence:

>1590_bases
GTGGCTGACTCTGCTGGCCTCGACCAGCCCGAACCCTCGCCCCGCCCCGTGTTGGTGGTCGACTTCGGTGCGCAGTATGC
ACAGCTTATTGCCCGCCGGGTTCGAGAAGCACGGGTTTTTTCCGAGGTAATTCCGCACACCACCTCGATCGAGGAGATCA
CCGCCCGTGATCCGCTGGCGATTGTGCTTTCTGGTGGACCGGCCAGTGTCTATACGGAAGGTGCTCCGCAGCTCGATCCA
GCGCTGTTTGACCTCGGTTTGCCGGTGTTCGGGATCTGCTACGGGTTTCAGGCCATGGCTCAGGTGCTCGGCGGGACTGT
CGCCCGAACCAAGACCAGTGAATACGGCCGGACGGAACTGAAAGTCCTTGGCGGTGATCTGCATTCGGGTCTTCCTGGTG
TCCAGCCGGTCTGGATGAGTCACGGTGACGCGGTCACCGCCGCGCCCGATGGATTCGACGTGGCTGCCAGCAGCTCGGGT
GCCCCAGTGGCCGCCTTCGAGAACCGGGACCGGCGTCTGGCCGGGGTGCAGTATCACCCGGAGGTGATGCACACCCCGCA
CGGGCAACAAGTACTCAGCCGGTTCTTGCACGATTTCGCTGGGCTTGACGCGGATTGGACGGCTGCCAACATCGCTGGTG
TGCTGGTCGAGCAGGTGCGTGCCCAGATCGGCAACGGCCATGCGATCTGCGGACTGTCTGGAGGTGTGGATTCCGCAGTG
GCCGCAGCACTGGTGCAACGCGCCATCGGAGACCGATTGACCTGTATCTTTGTCGATCATGGTCTGCTGCGTGACGGTGA
GCGCGGACAGGTGCAGCGTGATTTTGTCGCCGCTACTGGCGCTAAGCTGGTTACCGTCGACGCGGCCGAGACTTTCCTTC
AGGCGCTGTCGGGTGTGACCAACCCCGAAGGCAAGCGTAAGATTATCGGACGCCAGTTCATCCGGGCATTTGAAGGTGCA
GTACGGGATTTGTTGGGTGACAGCACTTTTGATTCCGGGATCGAGTTCTTGGTTCAGGGCACACTGTATCCGGATGTGGT
GGAGTCCGGTGGGGGCAGTGGCACTGCGAACATCAAGAGCCACCACAATGTTGGTGGTCTTCCCGACAACCTAAGGTTCA
AGCTCGTTGAGCCGCTGCGGCTGCTATTCAAAGACGAGGTGCGTGCGGTCGGTCGACAGTTGGACCTGCCGGAGGAAATT
GTTGCTCGCCAACCATTTCCGGGTCCTGGCCTGGGCATCCGGATCGTTGGTGAGGTGACTGCCGAACGGCTGGACACGCT
GCGGCGGGCCGACTCGATAGCGCGTGAGGAGCTGACCACGGCGGGTCTGGACTACCAGATTTGGCAGTGCCCGGTAGTGC
TGCTGGCCGACGTACGTTCGGTCGGTGTGCAGGGGGATAATCGCAGTTACGGACACCCGATCGTGCTGCGGCCAGTGTCT
AGCGAGGATGCCATGACAGCCGATTGGACCTGGGTTCCTTATGAGGTGTTGGAGTGCATCTCGACGCGTATCACCAACGA
GGTTGCTGAGGTTAACCGCGTGGTGTTGGACATCACCAACAAACCGCCCGGCACCATCGAGTGGGAATAG

Upstream 100 bases:

>100_bases
GGGAACAACAGGCGGTTGTCGGCGTGGAGTTGATCGCGAGGCAGCCCTGTGCGTTGCTGCTTGCCTCGGGAGCTGCTGCC
AGCCTCTAGACTGGCTGCCC

Downstream 100 bases:

>100_bases
CGGTCGCCGAAAACTTTCCGGAATGACATCACGTCGCTGGCGCTCCTCGGGCGCTCAGGGTTGCCGGTGAATGCGGAACC
GTTATTTGGTGTCGATGATG

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MADSAGLDQPEPSPRPVLVVDFGAQYAQLIARRVREARVFSEVIPHTTSIEEITARDPLAIVLSGGPASVYTEGAPQLDP
ALFDLGLPVFGICYGFQAMAQVLGGTVARTKTSEYGRTELKVLGGDLHSGLPGVQPVWMSHGDAVTAAPDGFDVAASSSG
APVAAFENRDRRLAGVQYHPEVMHTPHGQQVLSRFLHDFAGLDADWTAANIAGVLVEQVRAQIGNGHAICGLSGGVDSAV
AAALVQRAIGDRLTCIFVDHGLLRDGERGQVQRDFVAATGAKLVTVDAAETFLQALSGVTNPEGKRKIIGRQFIRAFEGA
VRDLLGDSTFDSGIEFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDNLRFKLVEPLRLLFKDEVRAVGRQLDLPEEI
VARQPFPGPGLGIRIVGEVTAERLDTLRRADSIAREELTTAGLDYQIWQCPVVLLADVRSVGVQGDNRSYGHPIVLRPVS
SEDAMTADWTWVPYEVLECISTRITNEVAEVNRVVLDITNKPPGTIEWE

Sequences:

>Translated_529_residues
MADSAGLDQPEPSPRPVLVVDFGAQYAQLIARRVREARVFSEVIPHTTSIEEITARDPLAIVLSGGPASVYTEGAPQLDP
ALFDLGLPVFGICYGFQAMAQVLGGTVARTKTSEYGRTELKVLGGDLHSGLPGVQPVWMSHGDAVTAAPDGFDVAASSSG
APVAAFENRDRRLAGVQYHPEVMHTPHGQQVLSRFLHDFAGLDADWTAANIAGVLVEQVRAQIGNGHAICGLSGGVDSAV
AAALVQRAIGDRLTCIFVDHGLLRDGERGQVQRDFVAATGAKLVTVDAAETFLQALSGVTNPEGKRKIIGRQFIRAFEGA
VRDLLGDSTFDSGIEFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDNLRFKLVEPLRLLFKDEVRAVGRQLDLPEEI
VARQPFPGPGLGIRIVGEVTAERLDTLRRADSIAREELTTAGLDYQIWQCPVVLLADVRSVGVQGDNRSYGHPIVLRPVS
SEDAMTADWTWVPYEVLECISTRITNEVAEVNRVVLDITNKPPGTIEWE
>Mature_528_residues
ADSAGLDQPEPSPRPVLVVDFGAQYAQLIARRVREARVFSEVIPHTTSIEEITARDPLAIVLSGGPASVYTEGAPQLDPA
LFDLGLPVFGICYGFQAMAQVLGGTVARTKTSEYGRTELKVLGGDLHSGLPGVQPVWMSHGDAVTAAPDGFDVAASSSGA
PVAAFENRDRRLAGVQYHPEVMHTPHGQQVLSRFLHDFAGLDADWTAANIAGVLVEQVRAQIGNGHAICGLSGGVDSAVA
AALVQRAIGDRLTCIFVDHGLLRDGERGQVQRDFVAATGAKLVTVDAAETFLQALSGVTNPEGKRKIIGRQFIRAFEGAV
RDLLGDSTFDSGIEFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDNLRFKLVEPLRLLFKDEVRAVGRQLDLPEEIV
ARQPFPGPGLGIRIVGEVTAERLDTLRRADSIAREELTTAGLDYQIWQCPVVLLADVRSVGVQGDNRSYGHPIVLRPVSS
EDAMTADWTWVPYEVLECISTRITNEVAEVNRVVLDITNKPPGTIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=550, Percent_Identity=35.8181818181818, Blast_Score=295, Evalue=7e-80,
Organism=Escherichia coli, GI1788854, Length=524, Percent_Identity=47.1374045801527, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1789760, Length=155, Percent_Identity=34.1935483870968, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71992717, Length=572, Percent_Identity=36.3636363636364, Blast_Score=291, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI71992710, Length=572, Percent_Identity=36.3636363636364, Blast_Score=290, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI133901714, Length=572, Percent_Identity=36.3636363636364, Blast_Score=290, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6323873, Length=517, Percent_Identity=45.8413926499033, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI281365319, Length=570, Percent_Identity=32.6315789473684, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_MYCLB (B8ZUE2)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503014.1
- ProteinModelPortal:   B8ZUE2
- EnsemblBacteria:   EBMYCT00000085713
- GeneID:   7326425
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015860
- HOGENOM:   HBG391775
- OMA:   PSTIEPC
- ProtClustDB:   PRK00074
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 56724; Mature: 56593

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 93-93 ACT_SITE 179-179 ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSAGLDQPEPSPRPVLVVDFGAQYAQLIARRVREARVFSEVIPHTTSIEEITARDPLA
CCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEE
IVLSGGPASVYTEGAPQLDPALFDLGLPVFGICYGFQAMAQVLGGTVARTKTSEYGRTEL
EEECCCCCCEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEE
KVLGGDLHSGLPGVQPVWMSHGDAVTAAPDGFDVAASSSGAPVAAFENRDRRLAGVQYHP
EEECCHHHCCCCCCCCHHCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCEECC
EVMHTPHGQQVLSRFLHDFAGLDADWTAANIAGVLVEQVRAQIGNGHAICGLSGGVDSAV
HHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHH
AAALVQRAIGDRLTCIFVDHGLLRDGERGQVQRDFVAATGAKLVTVDAAETFLQALSGVT
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHCCCC
NPEGKRKIIGRQFIRAFEGAVRDLLGDSTFDSGIEFLVQGTLYPDVVESGGGSGTANIKS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCC
HHNVGGLPDNLRFKLVEPLRLLFKDEVRAVGRQLDLPEEIVARQPFPGPGLGIRIVGEVT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEHHHH
AERLDTLRRADSIAREELTTAGLDYQIWQCPVVLLADVRSVGVQGDNRSYGHPIVLRPVS
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
SEDAMTADWTWVPYEVLECISTRITNEVAEVNRVVLDITNKPPGTIEWE
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ADSAGLDQPEPSPRPVLVVDFGAQYAQLIARRVREARVFSEVIPHTTSIEEITARDPLA
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEE
IVLSGGPASVYTEGAPQLDPALFDLGLPVFGICYGFQAMAQVLGGTVARTKTSEYGRTEL
EEECCCCCCEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEE
KVLGGDLHSGLPGVQPVWMSHGDAVTAAPDGFDVAASSSGAPVAAFENRDRRLAGVQYHP
EEECCHHHCCCCCCCCHHCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCEECC
EVMHTPHGQQVLSRFLHDFAGLDADWTAANIAGVLVEQVRAQIGNGHAICGLSGGVDSAV
HHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHH
AAALVQRAIGDRLTCIFVDHGLLRDGERGQVQRDFVAATGAKLVTVDAAETFLQALSGVT
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHCCCC
NPEGKRKIIGRQFIRAFEGAVRDLLGDSTFDSGIEFLVQGTLYPDVVESGGGSGTANIKS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCC
HHNVGGLPDNLRFKLVEPLRLLFKDEVRAVGRQLDLPEEIVARQPFPGPGLGIRIVGEVT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEHHHH
AERLDTLRRADSIAREELTTAGLDYQIWQCPVVLLADVRSVGVQGDNRSYGHPIVLRPVS
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
SEDAMTADWTWVPYEVLECISTRITNEVAEVNRVVLDITNKPPGTIEWE
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA