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The map label for this gene is espA [H]

Identifier: 221229602

GI number: 221229602

Start: 503226

End: 504410

Strand: Direct

Name: espA [H]

Synonym: MLBr_00405

Alternate gene names: 221229602

Gene position: 503226-504410 (Clockwise)

Preceding gene: 221229598

Following gene: 221229603

Centisome position: 15.4

GC content: 57.47

Gene sequence:

>1185_bases
ATGAGCGGCGCATTCATCATCGATCCGACGTTAAAGGCCATTGAAGCTTGGCATGCACTCCTGGGGATAGGAGTACCCAA
TGACGGGGGAGTGCTCTACTCTTCATTATCGTTCTTCGAAAAAGCGCTTGAGCACCTGGCGGCGGCGTTTCCTGGTGACG
GCTGGCTAGGCTCTGCCGCGGATAAATACGCCGGCCAAAACCGTAAACGGGTGGACATTTTTCAGGAGCTGGCTGAGCTG
GATAAAGAGCTTATCGAGCTAATCCATAACCAGGCCAACTCGGTCCAGACGACACGCGGCATCCTGGATGGCGCGAAGAA
GGCGCTCCTGTTCGTGCGTCCGGTGGCTATAGACCTGAACTACATCCCGCTTGTCGGATCTGTCATGTCGGCTTCCATAC
AGGCTCAGGCCTGCGCAGCTGCCATGGCCGCAGTGAGCGGCGGGCTTGCTTACCTACTCGTCCAGACTGCGATCCATACG
GCTAAATTCGTTGCTTTGCTGGCTCGGTTGGCGCACCTGTTGGCGTCCGCTGTCGCGGACGTAGTTTCGGACGGAGTAGC
GATCATAAAGGGCATTGTGGATCACCTATGGCATTTTATAGCGGGCGCGTTAACGGGCCTGAAGGATATTGTGGAAAAGA
TCATCCATTGGTTTTTTGGACTGTTCTCGCACTGGTGGTCGAGACTGCACTCCTTCTTTGGAGGCATCCCTGGCCTGAGT
GGTGCGACTAGCGGCTTGTCCCAGGTGACCGGTTTGTTTGGTGTTCCCGGTTTGGCCGGTTCGTCCGGGTTACTAAGCGG
CGAGAGCTTATTGAGCACAGAAAACTTACCCAGCCTGGCCGGAGTTGGGGCCGGGCTCGGCTTAGGGAGCTTGCCGCAAC
TGGCTCAGCTGCATGCCGCCTCGACTCGGCAGGGAACACGTTCCCAAGCCGGTGTTTCGGCCGAACTCTCTACGGAGCAG
TTTGGCGGACAGCAGGAGCCGGTCTCCGCACAGGGCTCTCAAGGTATGGGCGGCTCTCAAGGTATGGGCGGCATGACTCC
CGCTTCGACGAAGTCGAAAAAAGATGAGCGGAAGAAGAAGAAGTACTCGGAAGGCGCAGCAGCGGGCACAGACGATGCGG
AGCGTGCGCCAATCGAAGTTCAATCAGGCGGTGGTAAACGGGCGTTGGCACAACACGTCGTTTAA

Upstream 100 bases:

>100_bases
GGTCTGAAGGTAATCGTACAATATGCAATAACAGGTCAATATAAGTCATGGTACGGCATTATATTCTATAGCGACGACTG
TTAGGATGTAGGCGGCAGGC

Downstream 100 bases:

>100_bases
CAATATATGGCGAGCTAGATCCATTGTGAACCGGCGCTGTAGCAAGAGACGGAAGGGATGCGATCAATGATAGATAACTT
GACGGTTCAGAGCGAACACC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSGAFIIDPTLKAIEAWHALLGIGVPNDGGVLYSSLSFFEKALEHLAAAFPGDGWLGSAADKYAGQNRKRVDIFQELAEL
DKELIELIHNQANSVQTTRGILDGAKKALLFVRPVAIDLNYIPLVGSVMSASIQAQACAAAMAAVSGGLAYLLVQTAIHT
AKFVALLARLAHLLASAVADVVSDGVAIIKGIVDHLWHFIAGALTGLKDIVEKIIHWFFGLFSHWWSRLHSFFGGIPGLS
GATSGLSQVTGLFGVPGLAGSSGLLSGESLLSTENLPSLAGVGAGLGLGSLPQLAQLHAASTRQGTRSQAGVSAELSTEQ
FGGQQEPVSAQGSQGMGGSQGMGGMTPASTKSKKDERKKKKYSEGAAAGTDDAERAPIEVQSGGGKRALAQHVV

Sequences:

>Translated_394_residues
MSGAFIIDPTLKAIEAWHALLGIGVPNDGGVLYSSLSFFEKALEHLAAAFPGDGWLGSAADKYAGQNRKRVDIFQELAEL
DKELIELIHNQANSVQTTRGILDGAKKALLFVRPVAIDLNYIPLVGSVMSASIQAQACAAAMAAVSGGLAYLLVQTAIHT
AKFVALLARLAHLLASAVADVVSDGVAIIKGIVDHLWHFIAGALTGLKDIVEKIIHWFFGLFSHWWSRLHSFFGGIPGLS
GATSGLSQVTGLFGVPGLAGSSGLLSGESLLSTENLPSLAGVGAGLGLGSLPQLAQLHAASTRQGTRSQAGVSAELSTEQ
FGGQQEPVSAQGSQGMGGSQGMGGMTPASTKSKKDERKKKKYSEGAAAGTDDAERAPIEVQSGGGKRALAQHVV
>Mature_393_residues
SGAFIIDPTLKAIEAWHALLGIGVPNDGGVLYSSLSFFEKALEHLAAAFPGDGWLGSAADKYAGQNRKRVDIFQELAELD
KELIELIHNQANSVQTTRGILDGAKKALLFVRPVAIDLNYIPLVGSVMSASIQAQACAAAMAAVSGGLAYLLVQTAIHTA
KFVALLARLAHLLASAVADVVSDGVAIIKGIVDHLWHFIAGALTGLKDIVEKIIHWFFGLFSHWWSRLHSFFGGIPGLSG
ATSGLSQVTGLFGVPGLAGSSGLLSGESLLSTENLPSLAGVGAGLGLGSLPQLAQLHAASTRQGTRSQAGVSAELSTEQF
GGQQEPVSAQGSQGMGGSQGMGGMTPASTKSKKDERKKKKYSEGAAAGTDDAERAPIEVQSGGGKRALAQHVV

Specific function: Required for virulence. Required for secretion of EsxA (ESAT-6) and EsxB (CFP-10) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Probably secreted via the ESX-1 / type VII secretion system (T7SS) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40755; Mature: 40624

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
SGAFIIDPTLKAIEAWHALLGIGVPNDGGVLYSSLSFFEKALEHLAAAFPGDGWLGSAA
CCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
DKYAGQNRKRVDIFQELAELDKELIELIHNQANSVQTTRGILDGAKKALLFVRPVAIDLN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YIPLVGSVMSASIQAQACAAAMAAVSGGLAYLLVQTAIHTAKFVALLARLAHLLASAVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVSDGVAIIKGIVDHLWHFIAGALTGLKDIVEKIIHWFFGLFSHWWSRLHSFFGGIPGLS
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GATSGLSQVTGLFGVPGLAGSSGLLSGESLLSTENLPSLAGVGAGLGLGSLPQLAQLHAA
CHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHH
STRQGTRSQAGVSAELSTEQFGGQQEPVSAQGSQGMGGSQGMGGMTPASTKSKKDERKKK
HHCCCCHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
KYSEGAAAGTDDAERAPIEVQSGGGKRALAQHVV
HHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]