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The map label for this gene is mnmE [H]

Identifier: 220933188

GI number: 220933188

Start: 2574658

End: 2576049

Strand: Reverse

Name: mnmE [H]

Synonym: Hore_23560

Alternate gene names: 220933188

Gene position: 2576049-2574658 (Counterclockwise)

Preceding gene: 220933189

Following gene: 220933187

Centisome position: 99.92

GC content: 38.72

Gene sequence:

>1392_bases
ATGAAAGGGTTAACAGATGATACTATTGCAGCTATTTCTACACCTTTCGGGACTGCCGGTATTGGTAAAATCAGGGTCAG
CGGGTCTGGGGCTATAGATGTAGCTGACAGGATTTTTAAAGGAGTTAAAGATAAAAGTTTAAAGAATGTCAATACCTATA
CAGCTCACTACGGTTATGTTGTTGAGCCTGAATCCGGTAAAATGGTTGACGAGGTTATTGTTATTGTAATGAGGGGTCCC
CATTCTTTTACCGGTGAAGATGTAGTTGAGTTTGACTGCCACGGGGGTATGATTCCTTTAAAGAAAGTACTTGAGGTCTT
ATTAAATAATGGGGCCCGCCTGGCAGAACCCGGGGAGTTTTCAAAAAGGGCATTTTTAAATGGCAGGATTGATCTGGCCC
AGGCTGAGGGAATTATGGAGGTTATAAATTCAAAAACAGATAAAGGATTAGATGTGGCCATAAATCATTTAACCGGAAAG
CTTTCAAATAAAATTACTGGAATTAAAAATGAGGTTATTAGACTCCTGGCTCATCTTGAAGCCGCTATAGATTTTCCGGA
AGATGAAATTGAAGGCTTCGAACCTGATGAACTGGGTAACAGGATAAATGGTATAAAGGATGAAATTGAAGATTTACTGG
CAACAAGTGATCAGGGTAAAATATATCAGGAAGGTTTAAAGGCTGTAATTGTGGGAAAACCCAATGTCGGTAAATCGAGC
CTTTTAAACTCCCTTGTTCAGGAAAACAGGGCCATTGTTACTGATATACCCGGTACTACCAGAGATATAATAGAAGAGTA
TATAAATATAAGAGGTATCCCTTTAAGGATAATAGATACAGCCGGAATCAGGGAGACCCGGGATATGGTTGAAAAGATCG
GGGTTGAAAAAACCCGTAATTCCCTGAGGGAGGCCGATCTCGTCCTTTTTATGCTTGATGTAGCCCAGGGGTTGACTGAA
GAAGATATAAAAATATATGATATGGTTAAAGATAAACCGATGATTGTTCTTGTTAATAAGACAGACCTGGACAGGGAAAT
AGATGAAAAGAAAATTAAAGACCATTTTAAAGAACACCCGCTACTCTGGATTTCTGTTAAAGAAGAAATAGGGCTTGATA
ACCTTAAAGAGGCTATTATTGAGGAAGTAGTTTCTGAGGAGGTTAACGTTTCAGATAATATCTTTATAACCAGGGTCAGG
CATAAAGATGCTATGAAGAAAGCTGTTCATAGTATTAACAGGGTAAAAGAATCTTTAAACCAGGGGTTACCCTATGATTT
CCTTACTATAGACCTTAAAGACTGTCTTGATGCTCTGGGTGAGATTACTGGAGAGACGGTTACTGAGGATATTATTGATC
GGATTTTTGCTGATTTTTGTCTTGGTAAATAA

Upstream 100 bases:

>100_bases
ACAATGGATAATAACTGAATTTGTGTTTACACTAAACCCAGTAGGTTTTCCTACTGGGTTTCATTCGTATTTTTAAGGTT
TGATTCAAGGGGGTGGCGAG

Downstream 100 bases:

>100_bases
CGAAGATGTTGTAGTTAAAGGAGGTAATAAGATGAGCAGGGATTTTATGTCTATCTATCCCAAAGAATATGATGTTATTG
TTATCGGGGCCGGACATGCC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKGLTDDTIAAISTPFGTAGIGKIRVSGSGAIDVADRIFKGVKDKSLKNVNTYTAHYGYVVEPESGKMVDEVIVIVMRGP
HSFTGEDVVEFDCHGGMIPLKKVLEVLLNNGARLAEPGEFSKRAFLNGRIDLAQAEGIMEVINSKTDKGLDVAINHLTGK
LSNKITGIKNEVIRLLAHLEAAIDFPEDEIEGFEPDELGNRINGIKDEIEDLLATSDQGKIYQEGLKAVIVGKPNVGKSS
LLNSLVQENRAIVTDIPGTTRDIIEEYINIRGIPLRIIDTAGIRETRDMVEKIGVEKTRNSLREADLVLFMLDVAQGLTE
EDIKIYDMVKDKPMIVLVNKTDLDREIDEKKIKDHFKEHPLLWISVKEEIGLDNLKEAIIEEVVSEEVNVSDNIFITRVR
HKDAMKKAVHSINRVKESLNQGLPYDFLTIDLKDCLDALGEITGETVTEDIIDRIFADFCLGK

Sequences:

>Translated_463_residues
MKGLTDDTIAAISTPFGTAGIGKIRVSGSGAIDVADRIFKGVKDKSLKNVNTYTAHYGYVVEPESGKMVDEVIVIVMRGP
HSFTGEDVVEFDCHGGMIPLKKVLEVLLNNGARLAEPGEFSKRAFLNGRIDLAQAEGIMEVINSKTDKGLDVAINHLTGK
LSNKITGIKNEVIRLLAHLEAAIDFPEDEIEGFEPDELGNRINGIKDEIEDLLATSDQGKIYQEGLKAVIVGKPNVGKSS
LLNSLVQENRAIVTDIPGTTRDIIEEYINIRGIPLRIIDTAGIRETRDMVEKIGVEKTRNSLREADLVLFMLDVAQGLTE
EDIKIYDMVKDKPMIVLVNKTDLDREIDEKKIKDHFKEHPLLWISVKEEIGLDNLKEAIIEEVVSEEVNVSDNIFITRVR
HKDAMKKAVHSINRVKESLNQGLPYDFLTIDLKDCLDALGEITGETVTEDIIDRIFADFCLGK
>Mature_463_residues
MKGLTDDTIAAISTPFGTAGIGKIRVSGSGAIDVADRIFKGVKDKSLKNVNTYTAHYGYVVEPESGKMVDEVIVIVMRGP
HSFTGEDVVEFDCHGGMIPLKKVLEVLLNNGARLAEPGEFSKRAFLNGRIDLAQAEGIMEVINSKTDKGLDVAINHLTGK
LSNKITGIKNEVIRLLAHLEAAIDFPEDEIEGFEPDELGNRINGIKDEIEDLLATSDQGKIYQEGLKAVIVGKPNVGKSS
LLNSLVQENRAIVTDIPGTTRDIIEEYINIRGIPLRIIDTAGIRETRDMVEKIGVEKTRNSLREADLVLFMLDVAQGLTE
EDIKIYDMVKDKPMIVLVNKTDLDREIDEKKIKDHFKEHPLLWISVKEEIGLDNLKEAIIEEVVSEEVNVSDNIFITRVR
HKDAMKKAVHSINRVKESLNQGLPYDFLTIDLKDCLDALGEITGETVTEDIIDRIFADFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=33.1914893617021, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=31.8725099601594, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=32.6797385620915, Blast_Score=212, Evalue=5e-55,
Organism=Escherichia coli, GI2367268, Length=467, Percent_Identity=35.9743040685225, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI87082120, Length=189, Percent_Identity=32.8042328042328, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=467, Percent_Identity=29.7644539614561, Blast_Score=185, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=32.1931589537223, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45550813, Length=481, Percent_Identity=34.5114345114345, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 51329; Mature: 51329

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLTDDTIAAISTPFGTAGIGKIRVSGSGAIDVADRIFKGVKDKSLKNVNTYTAHYGYV
CCCCCCCHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCEE
VEPESGKMVDEVIVIVMRGPHSFTGEDVVEFDCHGGMIPLKKVLEVLLNNGARLAEPGEF
EECCCCCHHHHEEEEEECCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCH
SKRAFLNGRIDLAQAEGIMEVINSKTDKGLDVAINHLTGKLSNKITGIKNEVIRLLAHLE
HHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIDFPEDEIEGFEPDELGNRINGIKDEIEDLLATSDQGKIYQEGLKAVIVGKPNVGKSS
HHCCCCHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCHHH
LLNSLVQENRAIVTDIPGTTRDIIEEYINIRGIPLRIIDTAGIRETRDMVEKIGVEKTRN
HHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHH
SLREADLVLFMLDVAQGLTEEDIKIYDMVKDKPMIVLVNKTDLDREIDEKKIKDHFKEHP
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
LLWISVKEEIGLDNLKEAIIEEVVSEEVNVSDNIFITRVRHKDAMKKAVHSINRVKESLN
EEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
QGLPYDFLTIDLKDCLDALGEITGETVTEDIIDRIFADFCLGK
CCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKGLTDDTIAAISTPFGTAGIGKIRVSGSGAIDVADRIFKGVKDKSLKNVNTYTAHYGYV
CCCCCCCHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCEE
VEPESGKMVDEVIVIVMRGPHSFTGEDVVEFDCHGGMIPLKKVLEVLLNNGARLAEPGEF
EECCCCCHHHHEEEEEECCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCH
SKRAFLNGRIDLAQAEGIMEVINSKTDKGLDVAINHLTGKLSNKITGIKNEVIRLLAHLE
HHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIDFPEDEIEGFEPDELGNRINGIKDEIEDLLATSDQGKIYQEGLKAVIVGKPNVGKSS
HHCCCCHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCHHH
LLNSLVQENRAIVTDIPGTTRDIIEEYINIRGIPLRIIDTAGIRETRDMVEKIGVEKTRN
HHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHH
SLREADLVLFMLDVAQGLTEEDIKIYDMVKDKPMIVLVNKTDLDREIDEKKIKDHFKEHP
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
LLWISVKEEIGLDNLKEAIIEEVVSEEVNVSDNIFITRVRHKDAMKKAVHSINRVKESLN
EEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
QGLPYDFLTIDLKDCLDALGEITGETVTEDIIDRIFADFCLGK
CCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA