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The map label for this gene is mnmG [H]

Identifier: 220933187

GI number: 220933187

Start: 2572725

End: 2574611

Strand: Reverse

Name: mnmG [H]

Synonym: Hore_23550

Alternate gene names: 220933187

Gene position: 2574611-2572725 (Counterclockwise)

Preceding gene: 220933188

Following gene: 220933186

Centisome position: 99.86

GC content: 43.67

Gene sequence:

>1887_bases
ATGTCTATCTATCCCAAAGAATATGATGTTATTGTTATCGGGGCCGGACATGCCGGGTCTGAGGCATCACTGGCACCTGC
CAGAATGGGCTTTAAAGTTCTGACCATGACTGTAGACCTGGACCATGTTGCCTTTATGCCCTGTAATCCATCTCTGGGTG
GTCCCGGTAAGTCCCATATTGTCCGGGAGATTGATGCCCTCGGGGGAGAAATGGCCCGTAACATGGATGAAACCATGATC
CAGATCAGAATGCTTAATACCAGCAAGGGACCAGCCGTCCGTGGTTTGCGGGGACAGGCTGATAAAAAACAGTACCACCT
GAGGATGAAGCGTGTCCTCGAAAGGGAGCCAAATGTTGATTTAAAGCAGGAAATTGCAGAAAAACTGGTCATAGAAGATG
GAGAAATAAAAGGGGTTATAACCAAAACCGGTATTCTATACAGAGCTAAAAAAGTCATTGTTACTACGGGTACCTTTTTA
AAGGGAAAAATCATCATCGGGGAGGCTACTTTTAATGCCGGGCCCAATCAGCAGTATCCGGCCAATAAGCTATCAGAATC
CTTAAAGAAGGCTGGCTTTAAACTTCGACGGTTTAAAACTGGAACCCCGCCCCGGGTTAATAAGAATTCCCTTGATTTTT
CTAAAATGAAAGAGCAGACCGGGGATAAGGGGTTGACCTTCTCTTTTGTCTCCCCACCCTTTACCAGGGAACAGGTTTCC
TGCTGGTTGACATATACAACAGATGAGACCCATCGTATTATCCAGGAAAACAAGCACCGTACTCCACTTTTCAGTGGTGA
TATTGAAGGAGTAGGGCCCAGGTACTGCCCCTCTATTGAGGATAAAGTAGTTCGCTTCCCACATAAAGACAGGCATCAAA
TCTTTCTAGAACCTGAAGGGGAGTTTACCGATGAATATTATGTAGCTGGACTTTCAAGCAGCTTACCTGAAGATGTTCAG
ATTGATCTGGTCAGGTCGATACCGGGTATGGAAAAGGCTGAGATTATGCGTCCCGCCTATGCCATTGAATATGACTGTAT
TGATCCCACTCAGTTAAAGCTAACCCTGGAGGCCAAACACATTAAAGGCCTTTATATGGCCGGTCAGATTAATGGGACTT
CCGGTTATGAGGAGGCTGCCGGGCAGGGTCTGATTGCCGGTATTAATGCATCCTTAAGTTTACAGGGTAAAGATCCTTTA
ATACTGAAGCGTTCTGAAGCCTATATCGGGGTTTTGATTGATGAACTGGTTACTAAAGGGACCAATGAACCATACCGGAT
TATGACCTCAAGGGCAGAGTACCGTCTTATTTTACGACAGGATAATGCTGACCAGCGATTGACTCCAAAAGGTTATGAAA
TTGGTTTGATTTCTGAAGAGAGGTATAAAAAATTCAGGAAAAAGGTAGATAAGATTGAAGAGGCCCTTGAATTTTTGAGG
TCAAATCAGATCAGTCCTACTCCGGAGGTCAGAGAACGACTAAAGGAATTAAATAGTGGGGATTTGAGTAAACCGGTCAC
CCTGGAACAGCTGTTAAGGAGACCTCAACTTGAATACAGAGACCTGAAATATTTTGTTGATGATTTACCTGAGTATCCTG
ATGATATTATAGAACAGGTCTCAATTCAGGTTAAATATAAAGGGTATATTGACAGACAGATGTCCCAGATAGAACAGTTT
AAAAAAATGGAGGATAAATTAATACCTGATGATATTGATTATCAGGAACTGGAGAACCTCCGTTTGGAGGCCAGGGAAAA
ACTTGATAAAATAAAACCCCGTTCCCTTGGGCAGGCTTCCAGAATTTCAGGTGTATCCCCGGCTGATATTTCAGTATTAA
TGGTTTATTTAGAACAGCGGCAGAGGAGGAAACAGGGTGAAAAGTAA

Upstream 100 bases:

>100_bases
GTTACTGAGGATATTATTGATCGGATTTTTGCTGATTTTTGTCTTGGTAAATAACGAAGATGTTGTAGTTAAAGGAGGTA
ATAAGATGAGCAGGGATTTT

Downstream 100 bases:

>100_bases
TAAGTTTTATTCCCGGATAGCTGCAGGCCTTGAGGCCATGAATATTGAGTATCAGGAATATATGATTGACCAACTCCAGG
ATTATCTAAAATTTTTAAAG

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MSIYPKEYDVIVIGAGHAGSEASLAPARMGFKVLTMTVDLDHVAFMPCNPSLGGPGKSHIVREIDALGGEMARNMDETMI
QIRMLNTSKGPAVRGLRGQADKKQYHLRMKRVLEREPNVDLKQEIAEKLVIEDGEIKGVITKTGILYRAKKVIVTTGTFL
KGKIIIGEATFNAGPNQQYPANKLSESLKKAGFKLRRFKTGTPPRVNKNSLDFSKMKEQTGDKGLTFSFVSPPFTREQVS
CWLTYTTDETHRIIQENKHRTPLFSGDIEGVGPRYCPSIEDKVVRFPHKDRHQIFLEPEGEFTDEYYVAGLSSSLPEDVQ
IDLVRSIPGMEKAEIMRPAYAIEYDCIDPTQLKLTLEAKHIKGLYMAGQINGTSGYEEAAGQGLIAGINASLSLQGKDPL
ILKRSEAYIGVLIDELVTKGTNEPYRIMTSRAEYRLILRQDNADQRLTPKGYEIGLISEERYKKFRKKVDKIEEALEFLR
SNQISPTPEVRERLKELNSGDLSKPVTLEQLLRRPQLEYRDLKYFVDDLPEYPDDIIEQVSIQVKYKGYIDRQMSQIEQF
KKMEDKLIPDDIDYQELENLRLEAREKLDKIKPRSLGQASRISGVSPADISVLMVYLEQRQRRKQGEK

Sequences:

>Translated_628_residues
MSIYPKEYDVIVIGAGHAGSEASLAPARMGFKVLTMTVDLDHVAFMPCNPSLGGPGKSHIVREIDALGGEMARNMDETMI
QIRMLNTSKGPAVRGLRGQADKKQYHLRMKRVLEREPNVDLKQEIAEKLVIEDGEIKGVITKTGILYRAKKVIVTTGTFL
KGKIIIGEATFNAGPNQQYPANKLSESLKKAGFKLRRFKTGTPPRVNKNSLDFSKMKEQTGDKGLTFSFVSPPFTREQVS
CWLTYTTDETHRIIQENKHRTPLFSGDIEGVGPRYCPSIEDKVVRFPHKDRHQIFLEPEGEFTDEYYVAGLSSSLPEDVQ
IDLVRSIPGMEKAEIMRPAYAIEYDCIDPTQLKLTLEAKHIKGLYMAGQINGTSGYEEAAGQGLIAGINASLSLQGKDPL
ILKRSEAYIGVLIDELVTKGTNEPYRIMTSRAEYRLILRQDNADQRLTPKGYEIGLISEERYKKFRKKVDKIEEALEFLR
SNQISPTPEVRERLKELNSGDLSKPVTLEQLLRRPQLEYRDLKYFVDDLPEYPDDIIEQVSIQVKYKGYIDRQMSQIEQF
KKMEDKLIPDDIDYQELENLRLEAREKLDKIKPRSLGQASRISGVSPADISVLMVYLEQRQRRKQGEK
>Mature_627_residues
SIYPKEYDVIVIGAGHAGSEASLAPARMGFKVLTMTVDLDHVAFMPCNPSLGGPGKSHIVREIDALGGEMARNMDETMIQ
IRMLNTSKGPAVRGLRGQADKKQYHLRMKRVLEREPNVDLKQEIAEKLVIEDGEIKGVITKTGILYRAKKVIVTTGTFLK
GKIIIGEATFNAGPNQQYPANKLSESLKKAGFKLRRFKTGTPPRVNKNSLDFSKMKEQTGDKGLTFSFVSPPFTREQVSC
WLTYTTDETHRIIQENKHRTPLFSGDIEGVGPRYCPSIEDKVVRFPHKDRHQIFLEPEGEFTDEYYVAGLSSSLPEDVQI
DLVRSIPGMEKAEIMRPAYAIEYDCIDPTQLKLTLEAKHIKGLYMAGQINGTSGYEEAAGQGLIAGINASLSLQGKDPLI
LKRSEAYIGVLIDELVTKGTNEPYRIMTSRAEYRLILRQDNADQRLTPKGYEIGLISEERYKKFRKKVDKIEEALEFLRS
NQISPTPEVRERLKELNSGDLSKPVTLEQLLRRPQLEYRDLKYFVDDLPEYPDDIIEQVSIQVKYKGYIDRQMSQIEQFK
KMEDKLIPDDIDYQELENLRLEAREKLDKIKPRSLGQASRISGVSPADISVLMVYLEQRQRRKQGEK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=645, Percent_Identity=40.7751937984496, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI19882217, Length=664, Percent_Identity=39.0060240963855, Blast_Score=431, Evalue=1e-120,
Organism=Homo sapiens, GI183227703, Length=685, Percent_Identity=38.3941605839416, Blast_Score=426, Evalue=1e-119,
Organism=Escherichia coli, GI2367273, Length=621, Percent_Identity=48.792270531401, Blast_Score=608, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17534255, Length=630, Percent_Identity=40.1587301587302, Blast_Score=450, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6321202, Length=637, Percent_Identity=41.7582417582418, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=41.8238993710692, Blast_Score=458, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 71283; Mature: 71152

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYPKEYDVIVIGAGHAGSEASLAPARMGFKVLTMTVDLDHVAFMPCNPSLGGPGKSHI
CCCCCCCCCEEEEECCCCCCCCCCCHHHCCEEEEEEEEEECCEEEECCCCCCCCCCHHHH
VREIDALGGEMARNMDETMIQIRMLNTSKGPAVRGLRGQADKKQYHLRMKRVLEREPNVD
HHHHHHHHHHHHHCHHHHEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
LKQEIAEKLVIEDGEIKGVITKTGILYRAKKVIVTTGTFLKGKIIIGEATFNAGPNQQYP
HHHHHHHHHCCCCCCEEEEEEECCCEEEEEEEEEEECCEEEEEEEEEEEECCCCCCCCCC
ANKLSESLKKAGFKLRRFKTGTPPRVNKNSLDFSKMKEQTGDKGLTFSFVSPPFTREQVS
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCEE
CWLTYTTDETHRIIQENKHRTPLFSGDIEGVGPRYCPSIEDKVVRFPHKDRHQIFLEPEG
EEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCEEEECCCC
EFTDEYYVAGLSSSLPEDVQIDLVRSIPGMEKAEIMRPAYAIEYDCIDPTQLKLTLEAKH
CCCCCCEEEECCCCCCCCHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCEEEEEEEEEC
IKGLYMAGQINGTSGYEEAAGQGLIAGINASLSLQGKDPLILKRSEAYIGVLIDELVTKG
CCEEEEEEEECCCCCHHHHCCCCEEEECCCEEEECCCCCEEEEECCCHHHHHHHHHHHCC
TNEPYRIMTSRAEYRLILRQDNADQRLTPKGYEIGLISEERYKKFRKKVDKIEEALEFLR
CCCCCEEEECCCEEEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
SNQISPTPEVRERLKELNSGDLSKPVTLEQLLRRPQLEYRDLKYFVDDLPEYPDDIIEQV
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHC
SIQVKYKGYIDRQMSQIEQFKKMEDKLIPDDIDYQELENLRLEAREKLDKIKPRSLGQAS
EEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
RISGVSPADISVLMVYLEQRQRRKQGEK
HCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIYPKEYDVIVIGAGHAGSEASLAPARMGFKVLTMTVDLDHVAFMPCNPSLGGPGKSHI
CCCCCCCCEEEEECCCCCCCCCCCHHHCCEEEEEEEEEECCEEEECCCCCCCCCCHHHH
VREIDALGGEMARNMDETMIQIRMLNTSKGPAVRGLRGQADKKQYHLRMKRVLEREPNVD
HHHHHHHHHHHHHCHHHHEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
LKQEIAEKLVIEDGEIKGVITKTGILYRAKKVIVTTGTFLKGKIIIGEATFNAGPNQQYP
HHHHHHHHHCCCCCCEEEEEEECCCEEEEEEEEEEECCEEEEEEEEEEEECCCCCCCCCC
ANKLSESLKKAGFKLRRFKTGTPPRVNKNSLDFSKMKEQTGDKGLTFSFVSPPFTREQVS
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCEE
CWLTYTTDETHRIIQENKHRTPLFSGDIEGVGPRYCPSIEDKVVRFPHKDRHQIFLEPEG
EEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCEEEECCCC
EFTDEYYVAGLSSSLPEDVQIDLVRSIPGMEKAEIMRPAYAIEYDCIDPTQLKLTLEAKH
CCCCCCEEEECCCCCCCCHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCEEEEEEEEEC
IKGLYMAGQINGTSGYEEAAGQGLIAGINASLSLQGKDPLILKRSEAYIGVLIDELVTKG
CCEEEEEEEECCCCCHHHHCCCCEEEECCCEEEECCCCCEEEEECCCHHHHHHHHHHHCC
TNEPYRIMTSRAEYRLILRQDNADQRLTPKGYEIGLISEERYKKFRKKVDKIEEALEFLR
CCCCCEEEECCCEEEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
SNQISPTPEVRERLKELNSGDLSKPVTLEQLLRRPQLEYRDLKYFVDDLPEYPDDIIEQV
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHC
SIQVKYKGYIDRQMSQIEQFKKMEDKLIPDDIDYQELENLRLEAREKLDKIKPRSLGQAS
EEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
RISGVSPADISVLMVYLEQRQRRKQGEK
HCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA