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The map label for this gene is pflA [H]

Identifier: 220933148

GI number: 220933148

Start: 2529747

End: 2530490

Strand: Reverse

Name: pflA [H]

Synonym: Hore_23160

Alternate gene names: 220933148

Gene position: 2530490-2529747 (Counterclockwise)

Preceding gene: 220933149

Following gene: 220933143

Centisome position: 98.15

GC content: 45.16

Gene sequence:

>744_bases
ATGTCCACAGGTTTAATTCATTCAGTTGAAAGCATGGGCGTCCATGACGGGCCCGGTATTCGTTATGTTGTCTTTACCCA
GGGATGTCCCCTGCGCTGTCAGTACTGCCATAATCCCGATACCTGGATGCGTAAAGCAGGAAAGAGGGTAGAGGCTGGTG
AGCTTTTATTACGGGTTTTAAAATGTAAACCCTATATGGATAGCTCCGGGGGTGGGGTCACTATTTCAGGTGGGGAACCG
ACCCTTCAACCCGGGTTTGTCCGGGAATTATTAAAGGCCTGCAAAGAGCAGGGGATTCATACTGCCCTTGATACATCAGG
CTATGTTACCCCTGAAGTATTTTCTTCAATTTTGCCGTATATTGACCTTGTTCTGCTGGATATAAAACATATAAAAGAAG
AAAAACACCGGGACTTGACCGGTGTCAGCAATGAAAGAACCCTTGAACTGATAAGCCTTTTGGAAAAAGAAAAACAGCCC
TACTGGATCAGGCATGTTATTGTTCCCGGTATAACCGATAACCGGGATGACTTAAACCAGCTGGCTTCATATCTGTCTAA
TCTGAATGGACTTGAAAGAGTTGAGCTTTTGCCCTATCACAGGCTGGGAGTTTATAAGTGGCAGGAGCTTGGTTATGAAT
ATAGGCTTGAAGGGATTGAGCCCCCTTCCCGGGGACAGATGCAAGAAATAAAGGGGTTATTTATAAAAAAGGGAGTATCT
GCATCTATCGCCGTTCATGGATAA

Upstream 100 bases:

>100_bases
TTCGGGGTATGCTGTAAACTTCATTAAACTAACACCGGAGCAGCAGCAGGAAGTAATAGCCCGGACCTTTCATGAGAGTC
TTTAAGGGGAGTGTCTGTAA

Downstream 100 bases:

>100_bases
AATAAGTATATTTTAACTGGATAAAACAGGGGATTAGCTGGTTAATCTCACAGTATATAAGACTGATTCAGCTATCACTG
TTTCATATCATAAATCAGGG

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSTGLIHSVESMGVHDGPGIRYVVFTQGCPLRCQYCHNPDTWMRKAGKRVEAGELLLRVLKCKPYMDSSGGGVTISGGEP
TLQPGFVRELLKACKEQGIHTALDTSGYVTPEVFSSILPYIDLVLLDIKHIKEEKHRDLTGVSNERTLELISLLEKEKQP
YWIRHVIVPGITDNRDDLNQLASYLSNLNGLERVELLPYHRLGVYKWQELGYEYRLEGIEPPSRGQMQEIKGLFIKKGVS
ASIAVHG

Sequences:

>Translated_247_residues
MSTGLIHSVESMGVHDGPGIRYVVFTQGCPLRCQYCHNPDTWMRKAGKRVEAGELLLRVLKCKPYMDSSGGGVTISGGEP
TLQPGFVRELLKACKEQGIHTALDTSGYVTPEVFSSILPYIDLVLLDIKHIKEEKHRDLTGVSNERTLELISLLEKEKQP
YWIRHVIVPGITDNRDDLNQLASYLSNLNGLERVELLPYHRLGVYKWQELGYEYRLEGIEPPSRGQMQEIKGLFIKKGVS
ASIAVHG
>Mature_246_residues
STGLIHSVESMGVHDGPGIRYVVFTQGCPLRCQYCHNPDTWMRKAGKRVEAGELLLRVLKCKPYMDSSGGGVTISGGEPT
LQPGFVRELLKACKEQGIHTALDTSGYVTPEVFSSILPYIDLVLLDIKHIKEEKHRDLTGVSNERTLELISLLEKEKQPY
WIRHVIVPGITDNRDDLNQLASYLSNLNGLERVELLPYHRLGVYKWQELGYEYRLEGIEPPSRGQMQEIKGLFIKKGVSA
SIAVHG

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=237, Percent_Identity=46.8354430379747, Blast_Score=242, Evalue=1e-65,
Organism=Escherichia coli, GI1790389, Length=268, Percent_Identity=26.865671641791, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI226510931, Length=268, Percent_Identity=26.865671641791, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790839, Length=268, Percent_Identity=25.7462686567164, Blast_Score=88, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27728; Mature: 27597

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGLIHSVESMGVHDGPGIRYVVFTQGCPLRCQYCHNPDTWMRKAGKRVEAGELLLRVL
CCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHH
KCKPYMDSSGGGVTISGGEPTLQPGFVRELLKACKEQGIHTALDTSGYVTPEVFSSILPY
HCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
IDLVLLDIKHIKEEKHRDLTGVSNERTLELISLLEKEKQPYWIRHVIVPGITDNRDDLNQ
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHH
LASYLSNLNGLERVELLPYHRLGVYKWQELGYEYRLEGIEPPSRGQMQEIKGLFIKKGVS
HHHHHHCCCCCHHHHHCCHHHHCCHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCC
ASIAVHG
CEEEECC
>Mature Secondary Structure 
STGLIHSVESMGVHDGPGIRYVVFTQGCPLRCQYCHNPDTWMRKAGKRVEAGELLLRVL
CCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHH
KCKPYMDSSGGGVTISGGEPTLQPGFVRELLKACKEQGIHTALDTSGYVTPEVFSSILPY
HCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
IDLVLLDIKHIKEEKHRDLTGVSNERTLELISLLEKEKQPYWIRHVIVPGITDNRDDLNQ
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHH
LASYLSNLNGLERVELLPYHRLGVYKWQELGYEYRLEGIEPPSRGQMQEIKGLFIKKGVS
HHHHHHCCCCCHHHHHCCHHHHCCHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCC
ASIAVHG
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]