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The map label for this gene is pflB [H]

Identifier: 220933149

GI number: 220933149

Start: 2530506

End: 2532725

Strand: Reverse

Name: pflB [H]

Synonym: Hore_23170

Alternate gene names: 220933149

Gene position: 2532725-2530506 (Counterclockwise)

Preceding gene: 220933151

Following gene: 220933148

Centisome position: 98.24

GC content: 49.64

Gene sequence:

>2220_bases
ATGGCCTATAACTTTAAAGCAGGAAGATGGCAAAAAGAGATTGATGTCCGGGATTTTATTCAGCAAAATTATACTCCATA
TACAGGGGATAGTTCTTTTTTAGAGGGTCCAACCGACCGGACAAGACAACTCTGGGAAGAGTGTCAAAAACTATTATGGC
AGGAGATTGAAGAGGGAGGGGTGCTGGATATCGATGTCGATACTGTATCCGATATTAACGCCTATGAGGCCGGGTATATT
AACCGTGACCTGGAGAAGATTGTCGGCCTGCAGACCGATAAACCCCTGAAACGGACCCTCAATGTCTATGGTGGTATAAG
GATGGCCCGGCAGGCGGCAGAGGCCTACGGGTATAAGGTTAATGAGCAGATCGAAAAAGTATTTAACAACTACAGGAAAA
CCCATAATGATGGGGTTTATGATGTCTATACCCCCGAATTACGGGAGATAAGGCGTTCCGGGGTAATTACTGGCCTGCCC
GATGCCTATGGCCGGGGACGGATTATCGGGGATTACCGACGGGTGGCCCTTTACGGTATTGACAGGTTAATCGAGGAGAA
AGAAAAGGACCTGGCCCATCTTCCCACCAGGATGACCGAAGAAAATGTCAGGCTGCGGGAGGAGTTAAGTGAACAGATCA
AGGCCCTGAAAAAACTTAAAGGCCTGGGTAGAACATACGGCCTGGAACTTTCACGGCCGGCCCGGACTGCTCACGAGGCC
ATCCAGTGGACCTATATGGGATACCTGGCTGCCATTCAGGAAAACAACGGGGCTGCTATGTCCCTTGGCCGGGTATGCGA
GTTTTTTGATATCTATATTGAACATGACCTCAGGAAAGGGAAGTTAACAGAGGAAGAGGCCCAGGAATTAATCGATGATT
TTGTCATCAAGCTCAGGCTGGCCCGGCAGCTACGGACTCCTGAATACAATAAACTCTTCAGTGGGGATCCCATGTGGATT
ACGGTTGTCATCGGTGGTACTGGTATAGACGGACGCCCCCTGGTAACAAAGACCAGCTTCAGGATTTTAAATACCCTGTA
TAACCTCGGACCGGCTCCGGAACCCAACCTCACCGTTCTCTGGTCCCGGAGATTACCGGATGGGTTTAAGGAGTTCTGTT
GCCGGGTATCACGGGATACCAGTTCTATTCAGTATGAAAATGATGAATTGATGAGGGAATATTACGGGGATGATTATGCC
ATTGCCTGCTGTGTATCAGCCCTCAGGACCGGGAAAGAGATGCAGTTCTTCGGGGCCAGGTGTAACCTGCCCAAGACCCT
CCTGTATGCCCTCAATGGTGGCCGGGATGAATTAAGTGGGGTCCAGGTGGGGCCCGAATTTGCCCCATACACCGGAGATA
TACTTGAGTATGACCTGGTCATGGAGAGGTTTGATACCTTCCTCGACTGGCTGGTGGAAAAATATGTTGATGCCCTCAAT
ATTATCCACTATATGCATGACAAATATGCCTATGAACGGGTTCAGATGGCCTTAATGGATACCGAAGTCGACCGGGTAAT
GGGCTTCGGTATTGCCGGCCTCTCCATTGTGGCAGACTCTTTAAGTGCCATTAAATATGCTAAAGTAAGGCCGGTCCGGA
ACAGTGACGGCCTTATAACTTCATTCGATGTTGAAGGTGATCACCCCAGATACGGTAATGATGATGACCGGGTTGACAGC
CTGGCAGTTGAGGTCGTAAGGCGGTTTTTAACAAAGCTTAAGCAGCATAAACTCTACCGTAATGCCCGTCACACCCTGTC
AATTTTAACCATAACTTCAAATGTTGTCTATGGCACGAAGACCGGGGCCACCCCGGACGGCCGGGAGGCAGGACAGCCCT
TTGCCCCGGGGGCCAATCCCATGCACGGTCGTGACGAATCAGGGGCCCTTGCCTCACTGAATACGGTTGCAAAACTACCA
TACGAGTACTGCCAGGACGGTATTTCCAATACCTTTACCATCGTCCCCGGAGCCCTGGGTGTTAATGAAGAGCAGCGGAT
CAATAATCTGGTAGCTATTCTCGATGGTTACTTTACCAAAGGTGGTCACCACCTTAATGTTAATGTTCTCAACAGGGAAA
CCCTCCTGGATGCGGTCAACCACCCCGAGAAATACCCCCAGTTAACCATCAGGGTTTCGGGGTATGCTGTAAACTTCATT
AAACTAACACCGGAGCAGCAGCAGGAAGTAATAGCCCGGACCTTTCATGAGAGTCTTTAA

Upstream 100 bases:

>100_bases
CGACCGCCTGGGCAGATAGATTAAGTCTATTTGCCCGGGCGGTTTTTTTAATGTCCAGGTAATTGAAGTAAATACAAGCA
TATAAGGGGAGGGGATAATA

Downstream 100 bases:

>100_bases
GGGGAGTGTCTGTAAATGTCCACAGGTTTAATTCATTCAGTTGAAAGCATGGGCGTCCATGACGGGCCCGGTATTCGTTA
TGTTGTCTTTACCCAGGGAT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MAYNFKAGRWQKEIDVRDFIQQNYTPYTGDSSFLEGPTDRTRQLWEECQKLLWQEIEEGGVLDIDVDTVSDINAYEAGYI
NRDLEKIVGLQTDKPLKRTLNVYGGIRMARQAAEAYGYKVNEQIEKVFNNYRKTHNDGVYDVYTPELREIRRSGVITGLP
DAYGRGRIIGDYRRVALYGIDRLIEEKEKDLAHLPTRMTEENVRLREELSEQIKALKKLKGLGRTYGLELSRPARTAHEA
IQWTYMGYLAAIQENNGAAMSLGRVCEFFDIYIEHDLRKGKLTEEEAQELIDDFVIKLRLARQLRTPEYNKLFSGDPMWI
TVVIGGTGIDGRPLVTKTSFRILNTLYNLGPAPEPNLTVLWSRRLPDGFKEFCCRVSRDTSSIQYENDELMREYYGDDYA
IACCVSALRTGKEMQFFGARCNLPKTLLYALNGGRDELSGVQVGPEFAPYTGDILEYDLVMERFDTFLDWLVEKYVDALN
IIHYMHDKYAYERVQMALMDTEVDRVMGFGIAGLSIVADSLSAIKYAKVRPVRNSDGLITSFDVEGDHPRYGNDDDRVDS
LAVEVVRRFLTKLKQHKLYRNARHTLSILTITSNVVYGTKTGATPDGREAGQPFAPGANPMHGRDESGALASLNTVAKLP
YEYCQDGISNTFTIVPGALGVNEEQRINNLVAILDGYFTKGGHHLNVNVLNRETLLDAVNHPEKYPQLTIRVSGYAVNFI
KLTPEQQQEVIARTFHESL

Sequences:

>Translated_739_residues
MAYNFKAGRWQKEIDVRDFIQQNYTPYTGDSSFLEGPTDRTRQLWEECQKLLWQEIEEGGVLDIDVDTVSDINAYEAGYI
NRDLEKIVGLQTDKPLKRTLNVYGGIRMARQAAEAYGYKVNEQIEKVFNNYRKTHNDGVYDVYTPELREIRRSGVITGLP
DAYGRGRIIGDYRRVALYGIDRLIEEKEKDLAHLPTRMTEENVRLREELSEQIKALKKLKGLGRTYGLELSRPARTAHEA
IQWTYMGYLAAIQENNGAAMSLGRVCEFFDIYIEHDLRKGKLTEEEAQELIDDFVIKLRLARQLRTPEYNKLFSGDPMWI
TVVIGGTGIDGRPLVTKTSFRILNTLYNLGPAPEPNLTVLWSRRLPDGFKEFCCRVSRDTSSIQYENDELMREYYGDDYA
IACCVSALRTGKEMQFFGARCNLPKTLLYALNGGRDELSGVQVGPEFAPYTGDILEYDLVMERFDTFLDWLVEKYVDALN
IIHYMHDKYAYERVQMALMDTEVDRVMGFGIAGLSIVADSLSAIKYAKVRPVRNSDGLITSFDVEGDHPRYGNDDDRVDS
LAVEVVRRFLTKLKQHKLYRNARHTLSILTITSNVVYGTKTGATPDGREAGQPFAPGANPMHGRDESGALASLNTVAKLP
YEYCQDGISNTFTIVPGALGVNEEQRINNLVAILDGYFTKGGHHLNVNVLNRETLLDAVNHPEKYPQLTIRVSGYAVNFI
KLTPEQQQEVIARTFHESL
>Mature_738_residues
AYNFKAGRWQKEIDVRDFIQQNYTPYTGDSSFLEGPTDRTRQLWEECQKLLWQEIEEGGVLDIDVDTVSDINAYEAGYIN
RDLEKIVGLQTDKPLKRTLNVYGGIRMARQAAEAYGYKVNEQIEKVFNNYRKTHNDGVYDVYTPELREIRRSGVITGLPD
AYGRGRIIGDYRRVALYGIDRLIEEKEKDLAHLPTRMTEENVRLREELSEQIKALKKLKGLGRTYGLELSRPARTAHEAI
QWTYMGYLAAIQENNGAAMSLGRVCEFFDIYIEHDLRKGKLTEEEAQELIDDFVIKLRLARQLRTPEYNKLFSGDPMWIT
VVIGGTGIDGRPLVTKTSFRILNTLYNLGPAPEPNLTVLWSRRLPDGFKEFCCRVSRDTSSIQYENDELMREYYGDDYAI
ACCVSALRTGKEMQFFGARCNLPKTLLYALNGGRDELSGVQVGPEFAPYTGDILEYDLVMERFDTFLDWLVEKYVDALNI
IHYMHDKYAYERVQMALMDTEVDRVMGFGIAGLSIVADSLSAIKYAKVRPVRNSDGLITSFDVEGDHPRYGNDDDRVDSL
AVEVVRRFLTKLKQHKLYRNARHTLSILTITSNVVYGTKTGATPDGREAGQPFAPGANPMHGRDESGALASLNTVAKLPY
EYCQDGISNTFTIVPGALGVNEEQRINNLVAILDGYFTKGGHHLNVNVLNRETLLDAVNHPEKYPQLTIRVSGYAVNFIK
LTPEQQQEVIARTFHESL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=746, Percent_Identity=60.0536193029491, Blast_Score=944, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=746, Percent_Identity=59.2493297587131, Blast_Score=923, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=575, Percent_Identity=28.8695652173913, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1790388, Length=668, Percent_Identity=24.4011976047904, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788933, Length=61, Percent_Identity=80.327868852459, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84017; Mature: 83886

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYNFKAGRWQKEIDVRDFIQQNYTPYTGDSSFLEGPTDRTRQLWEECQKLLWQEIEEGG
CCCCCCCCCCHHHCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
VLDIDVDTVSDINAYEAGYINRDLEKIVGLQTDKPLKRTLNVYGGIRMARQAAEAYGYKV
EEEEECCCCCCCCCHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
NEQIEKVFNNYRKTHNDGVYDVYTPELREIRRSGVITGLPDAYGRGRIIGDYRRVALYGI
HHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHH
DRLIEEKEKDLAHLPTRMTEENVRLREELSEQIKALKKLKGLGRTYGLELSRPARTAHEA
HHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHH
IQWTYMGYLAAIQENNGAAMSLGRVCEFFDIYIEHDLRKGKLTEEEAQELIDDFVIKLRL
HHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ARQLRTPEYNKLFSGDPMWITVVIGGTGIDGRPLVTKTSFRILNTLYNLGPAPEPNLTVL
HHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEE
WSRRLPDGFKEFCCRVSRDTSSIQYENDELMREYYGDDYAIACCVSALRTGKEMQFFGAR
ECCCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCC
CNLPKTLLYALNGGRDELSGVQVGPEFAPYTGDILEYDLVMERFDTFLDWLVEKYVDALN
CCCCHHHHHHHCCCHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIHYMHDKYAYERVQMALMDTEVDRVMGFGIAGLSIVADSLSAIKYAKVRPVRNSDGLIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
SFDVEGDHPRYGNDDDRVDSLAVEVVRRFLTKLKQHKLYRNARHTLSILTITSNVVYGTK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEECC
TGATPDGREAGQPFAPGANPMHGRDESGALASLNTVAKLPYEYCQDGISNTFTIVPGALG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCEEEEECCCCC
VNEEQRINNLVAILDGYFTKGGHHLNVNVLNRETLLDAVNHPEKYPQLTIRVSGYAVNFI
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHCCCCCCCEEEEEEECEEEEEE
KLTPEQQQEVIARTFHESL
EECCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AYNFKAGRWQKEIDVRDFIQQNYTPYTGDSSFLEGPTDRTRQLWEECQKLLWQEIEEGG
CCCCCCCCCHHHCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
VLDIDVDTVSDINAYEAGYINRDLEKIVGLQTDKPLKRTLNVYGGIRMARQAAEAYGYKV
EEEEECCCCCCCCCHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
NEQIEKVFNNYRKTHNDGVYDVYTPELREIRRSGVITGLPDAYGRGRIIGDYRRVALYGI
HHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHH
DRLIEEKEKDLAHLPTRMTEENVRLREELSEQIKALKKLKGLGRTYGLELSRPARTAHEA
HHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHH
IQWTYMGYLAAIQENNGAAMSLGRVCEFFDIYIEHDLRKGKLTEEEAQELIDDFVIKLRL
HHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ARQLRTPEYNKLFSGDPMWITVVIGGTGIDGRPLVTKTSFRILNTLYNLGPAPEPNLTVL
HHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEE
WSRRLPDGFKEFCCRVSRDTSSIQYENDELMREYYGDDYAIACCVSALRTGKEMQFFGAR
ECCCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCC
CNLPKTLLYALNGGRDELSGVQVGPEFAPYTGDILEYDLVMERFDTFLDWLVEKYVDALN
CCCCHHHHHHHCCCHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIHYMHDKYAYERVQMALMDTEVDRVMGFGIAGLSIVADSLSAIKYAKVRPVRNSDGLIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
SFDVEGDHPRYGNDDDRVDSLAVEVVRRFLTKLKQHKLYRNARHTLSILTITSNVVYGTK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEECC
TGATPDGREAGQPFAPGANPMHGRDESGALASLNTVAKLPYEYCQDGISNTFTIVPGALG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCEEEEECCCCC
VNEEQRINNLVAILDGYFTKGGHHLNVNVLNRETLLDAVNHPEKYPQLTIRVSGYAVNFI
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHCCCCCCCEEEEEEECEEEEEE
KLTPEQQQEVIARTFHESL
EECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA