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The map label for this gene is pstS [H]

Identifier: 220932995

GI number: 220932995

Start: 2353768

End: 2354589

Strand: Reverse

Name: pstS [H]

Synonym: Hore_21620

Alternate gene names: 220932995

Gene position: 2354589-2353768 (Counterclockwise)

Preceding gene: 220932996

Following gene: 220932994

Centisome position: 91.33

GC content: 39.9

Gene sequence:

>822_bases
ATGTTTAATTCAAAGAATTTGATTGCTTTGCTGGTTATTGCATTGGTAACTTTATTGTCAGCCGGAGTATTAGCCTCTGA
TACTTTGTTTATTCAGGGCTCTTCTACCGTATTACCCATTGCTCAGAAAGCTGCGGAAGTTTATATGTCTAAACATGATG
TTCATATTTCTGTGAGGGGTGGCGGTTCCGGTAATGGTATTGCTGCTTTAATTGATGGAGCTGTTGATATAGCAGACGCT
TCCCGTTTTATTAAAAACAAAGAGGTAAGTGCAGCTATTGCCAATGGTATTTATCCTGTACCCCATAGAGTAGCCAAAGA
CTCTATTGCTGTAGTTGTTCACCCCGGTAACCCTGTATCTGACTTAACTGTAGAAGAGTTAAAGGGGATCTACACCGGTA
AAATTACTAACTGGAAAGAGCTGGGTGGTAAAGATCAGGAAATAGTTGTTGTCTCCCGTGACTCCAGTTCTGGTACCTTT
GCTGTATTTAATGACATTGCCAATGATGGAGAATTAAATAATGAGGCTTTAAGGGTTACCAGCAGGGCTCTGTTACAGGC
TTCTAACGGTGAAGCAAGGGCTACAGTTGCTGATACTCCTGGTGCTATCGGTTATATCGGTCTGGGTTACTTAAATGATG
ATGTAAAAGCTGTAAAATTAAATGGTCATCTGCCAAGTGACTCTCAGTACATGATTGTTCGTCCCTTGTATATGTTTACA
AATGGGTGGCCCAGAGGATTAACCAAGAAATTTATTGATTTTATCTTAAGCCCTGAAGGTCAGAAGATTGTTAAAGAAGT
AGGTTATGTTCCTTTATACTAA

Upstream 100 bases:

>100_bases
ATTTACTCCGTGTTAACCAGATCGTAATATGCCTTTAACATTGTCGTGCTATGATTATAAGCGAACGATAAAATAAAACT
GTAAGGGGGAAATTACTTAA

Downstream 100 bases:

>100_bases
AAGTAAGCCAGAATTAAAAACACACAACAAGAAGTCATATAAAAAACATTAAGTAAGAGTTAACATTACCAATTAGAATA
CCACCAGATAAGTTTATAAT

Product: phosphate binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MFNSKNLIALLVIALVTLLSAGVLASDTLFIQGSSTVLPIAQKAAEVYMSKHDVHISVRGGGSGNGIAALIDGAVDIADA
SRFIKNKEVSAAIANGIYPVPHRVAKDSIAVVVHPGNPVSDLTVEELKGIYTGKITNWKELGGKDQEIVVVSRDSSSGTF
AVFNDIANDGELNNEALRVTSRALLQASNGEARATVADTPGAIGYIGLGYLNDDVKAVKLNGHLPSDSQYMIVRPLYMFT
NGWPRGLTKKFIDFILSPEGQKIVKEVGYVPLY

Sequences:

>Translated_273_residues
MFNSKNLIALLVIALVTLLSAGVLASDTLFIQGSSTVLPIAQKAAEVYMSKHDVHISVRGGGSGNGIAALIDGAVDIADA
SRFIKNKEVSAAIANGIYPVPHRVAKDSIAVVVHPGNPVSDLTVEELKGIYTGKITNWKELGGKDQEIVVVSRDSSSGTF
AVFNDIANDGELNNEALRVTSRALLQASNGEARATVADTPGAIGYIGLGYLNDDVKAVKLNGHLPSDSQYMIVRPLYMFT
NGWPRGLTKKFIDFILSPEGQKIVKEVGYVPLY
>Mature_273_residues
MFNSKNLIALLVIALVTLLSAGVLASDTLFIQGSSTVLPIAQKAAEVYMSKHDVHISVRGGGSGNGIAALIDGAVDIADA
SRFIKNKEVSAAIANGIYPVPHRVAKDSIAVVVHPGNPVSDLTVEELKGIYTGKITNWKELGGKDQEIVVVSRDSSSGTF
AVFNDIANDGELNNEALRVTSRALLQASNGEARATVADTPGAIGYIGLGYLNDDVKAVKLNGHLPSDSQYMIVRPLYMFT
NGWPRGLTKKFIDFILSPEGQKIVKEVGYVPLY

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

None

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011862
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 29053; Mature: 29053

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNSKNLIALLVIALVTLLSAGVLASDTLFIQGSSTVLPIAQKAAEVYMSKHDVHISVRG
CCCCCHHHHHHHHHHHHHHHCCCEECCEEEEECCCCEEHHHHHHHHHHHCCCCEEEEEEC
GGSGNGIAALIDGAVDIADASRFIKNKEVSAAIANGIYPVPHRVAKDSIAVVVHPGNPVS
CCCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHCCCCEEEEEECCCCCC
DLTVEELKGIYTGKITNWKELGGKDQEIVVVSRDSSSGTFAVFNDIANDGELNNEALRVT
CCCHHHHCCEEECCCCCHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHH
SRALLQASNGEARATVADTPGAIGYIGLGYLNDDVKAVKLNGHLPSDSQYMIVRPLYMFT
HHHHHCCCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCEEEEEEEEEEC
NGWPRGLTKKFIDFILSPEGQKIVKEVGYVPLY
CCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MFNSKNLIALLVIALVTLLSAGVLASDTLFIQGSSTVLPIAQKAAEVYMSKHDVHISVRG
CCCCCHHHHHHHHHHHHHHHCCCEECCEEEEECCCCEEHHHHHHHHHHHCCCCEEEEEEC
GGSGNGIAALIDGAVDIADASRFIKNKEVSAAIANGIYPVPHRVAKDSIAVVVHPGNPVS
CCCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHCCCCEEEEEECCCCCC
DLTVEELKGIYTGKITNWKELGGKDQEIVVVSRDSSSGTFAVFNDIANDGELNNEALRVT
CCCHHHHCCEEECCCCCHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHH
SRALLQASNGEARATVADTPGAIGYIGLGYLNDDVKAVKLNGHLPSDSQYMIVRPLYMFT
HHHHHCCCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCEEEEEEEEEEC
NGWPRGLTKKFIDFILSPEGQKIVKEVGYVPLY
CCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]