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The map label for this gene is purR [H]

Identifier: 220932885

GI number: 220932885

Start: 2217095

End: 2218120

Strand: Reverse

Name: purR [H]

Synonym: Hore_20530

Alternate gene names: 220932885

Gene position: 2218120-2217095 (Counterclockwise)

Preceding gene: 220932886

Following gene: 220932884

Centisome position: 86.04

GC content: 37.52

Gene sequence:

>1026_bases
ATGGCAGTTACTTTAAAAGATGTGGCGAAAAAGGCCGGGGTTTCACTGGCGACAACCTCTCTGGCTTTAAATGAAAGTGA
TAAAGTTAAAGAAGAAACTTATCTAAAAGTTCACCGGGTAGCCCGGGAGTTAAATTACATACCAAATGCCAGAGCCCGTG
CTCTGGTTAAAAGAAAAACAAGAGCCGTGGGACTGGTAATACCTGAAGTTGAAAACCCATTTTTTGCTGAGTTATCTCAG
GCAATAAAGGATACTTTGCATAAAAAGAAATATAATGTAATTCTGTGTAGTACAGATTATAAAACCGAAGAAGAAATCAG
GTATATTAACCTTTTTAAAAGCGGTATGGTTGATGGTGCCATTTTTGCCTGTCTTGGTGAGATGATGAAAGGAAATAATG
AGCTAATCCTGGACCTTGCCAGAAACCATGTCCCTGTTGTTTATATTGACAGGGAAAGTTCAGATAATGAACTTATACCC
GTTATTAAATCTGATTTAAAAGAAGGTTCTTATAAACTGACCAAATACTTAATTGAACTGGGGCATGAGGATATAGGGTT
TGTCGGTCAGAGCCTTGAAAGGATGGAAGGCTATAAAAAGGCTATGGCTGAATACAATTTACCGGTAAAAGATAAGTTCC
TGTTTTATAACTATCTTACCATTGAGGGTGGCATTGAAGTAGGCACAAAACTGCTTAACTATGACCGACTTCCCACAGCT
CTGGTTTGTTTAAATGACGAGATGGCTATCGGAGTTTTACAGGCTTTAACCAGGGAAGGAATCAGGGTTCCAGAGGATAT
TTCTATCTGTGGGATTGATAATATTAAAATATCAAACTTTTATAACCCTCCCCTGACTACAGTAAATGTTCCCAAAAAAG
AGATGGGGAAAAAGGCGGCAGAACTGCTCCTTAAACTAATATTTGAGGAGCATGTTGATAAAAATGAACAATTTATAATA
TACAACACCGACTTAATTATCAGACGTTCAGCTACATCACCCCGGAAAACAAATATATTATTATAA

Upstream 100 bases:

>100_bases
AAAAATAATTTTCAAAAAATTTGACTTTATGAAAATATTTATATATAATATATTTGTAACAAAATTTAAAACGTTTTAAA
AATTAAAAAGGACTGGTCTT

Downstream 100 bases:

>100_bases
GTTGATTAAATTCCACCAGTTCTATTTTTATTTTTATATAATATAGGAGGTGGTTATAAAACGGGTTTTTTTCTTTTTAT
ATTTTAAAACGTTTTAATTT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MAVTLKDVAKKAGVSLATTSLALNESDKVKEETYLKVHRVARELNYIPNARARALVKRKTRAVGLVIPEVENPFFAELSQ
AIKDTLHKKKYNVILCSTDYKTEEEIRYINLFKSGMVDGAIFACLGEMMKGNNELILDLARNHVPVVYIDRESSDNELIP
VIKSDLKEGSYKLTKYLIELGHEDIGFVGQSLERMEGYKKAMAEYNLPVKDKFLFYNYLTIEGGIEVGTKLLNYDRLPTA
LVCLNDEMAIGVLQALTREGIRVPEDISICGIDNIKISNFYNPPLTTVNVPKKEMGKKAAELLLKLIFEEHVDKNEQFII
YNTDLIIRRSATSPRKTNILL

Sequences:

>Translated_341_residues
MAVTLKDVAKKAGVSLATTSLALNESDKVKEETYLKVHRVARELNYIPNARARALVKRKTRAVGLVIPEVENPFFAELSQ
AIKDTLHKKKYNVILCSTDYKTEEEIRYINLFKSGMVDGAIFACLGEMMKGNNELILDLARNHVPVVYIDRESSDNELIP
VIKSDLKEGSYKLTKYLIELGHEDIGFVGQSLERMEGYKKAMAEYNLPVKDKFLFYNYLTIEGGIEVGTKLLNYDRLPTA
LVCLNDEMAIGVLQALTREGIRVPEDISICGIDNIKISNFYNPPLTTVNVPKKEMGKKAAELLLKLIFEEHVDKNEQFII
YNTDLIIRRSATSPRKTNILL
>Mature_340_residues
AVTLKDVAKKAGVSLATTSLALNESDKVKEETYLKVHRVARELNYIPNARARALVKRKTRAVGLVIPEVENPFFAELSQA
IKDTLHKKKYNVILCSTDYKTEEEIRYINLFKSGMVDGAIFACLGEMMKGNNELILDLARNHVPVVYIDRESSDNELIPV
IKSDLKEGSYKLTKYLIELGHEDIGFVGQSLERMEGYKKAMAEYNLPVKDKFLFYNYLTIEGGIEVGTKLLNYDRLPTAL
VCLNDEMAIGVLQALTREGIRVPEDISICGIDNIKISNFYNPPLTTVNVPKKEMGKKAAELLLKLIFEEHVDKNEQFIIY
NTDLIIRRSATSPRKTNILL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=32.8358208955224, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=33.1360946745562, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=32.5878594249201, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1789202, Length=315, Percent_Identity=32.3809523809524, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1789068, Length=321, Percent_Identity=31.1526479750779, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=27.9874213836478, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788474, Length=315, Percent_Identity=29.5238095238095, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1786540, Length=340, Percent_Identity=26.7647058823529, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787906, Length=330, Percent_Identity=25.1515151515152, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=29.2063492063492, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786268, Length=309, Percent_Identity=24.5954692556634, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1790715, Length=317, Percent_Identity=26.4984227129338, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789456, Length=318, Percent_Identity=27.3584905660377, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790689, Length=321, Percent_Identity=22.7414330218069, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38551; Mature: 38420

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTLKDVAKKAGVSLATTSLALNESDKVKEETYLKVHRVARELNYIPNARARALVKRKT
CEEEHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RAVGLVIPEVENPFFAELSQAIKDTLHKKKYNVILCSTDYKTEEEIRYINLFKSGMVDGA
HEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHH
IFACLGEMMKGNNELILDLARNHVPVVYIDRESSDNELIPVIKSDLKEGSYKLTKYLIEL
HHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
GHEDIGFVGQSLERMEGYKKAMAEYNLPVKDKFLFYNYLTIEGGIEVGTKLLNYDRLPTA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCHHHHHHHHCCCCCCEE
LVCLNDEMAIGVLQALTREGIRVPEDISICGIDNIKISNFYNPPLTTVNVPKKEMGKKAA
EEEECCHHHHHHHHHHHHCCCCCCCCCEEECCCCEEECCCCCCCCCEEECCHHHHHHHHH
ELLLKLIFEEHVDKNEQFIIYNTDLIIRRSATSPRKTNILL
HHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AVTLKDVAKKAGVSLATTSLALNESDKVKEETYLKVHRVARELNYIPNARARALVKRKT
EEEHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RAVGLVIPEVENPFFAELSQAIKDTLHKKKYNVILCSTDYKTEEEIRYINLFKSGMVDGA
HEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHH
IFACLGEMMKGNNELILDLARNHVPVVYIDRESSDNELIPVIKSDLKEGSYKLTKYLIEL
HHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
GHEDIGFVGQSLERMEGYKKAMAEYNLPVKDKFLFYNYLTIEGGIEVGTKLLNYDRLPTA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCHHHHHHHHCCCCCCEE
LVCLNDEMAIGVLQALTREGIRVPEDISICGIDNIKISNFYNPPLTTVNVPKKEMGKKAA
EEEECCHHHHHHHHHHHHCCCCCCCCCEEECCCCEEECCCCCCCCCEEECCHHHHHHHHH
ELLLKLIFEEHVDKNEQFIIYNTDLIIRRSATSPRKTNILL
HHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA