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The map label for this gene is aprN [H]

Identifier: 220932760

GI number: 220932760

Start: 2059635

End: 2060966

Strand: Reverse

Name: aprN [H]

Synonym: Hore_19260

Alternate gene names: 220932760

Gene position: 2060966-2059635 (Counterclockwise)

Preceding gene: 220932761

Following gene: 220932758

Centisome position: 79.94

GC content: 47.75

Gene sequence:

>1332_bases
ATGAAAAAATTTCTAACTTTAACTCTGGTTACCCTTCTGGCTGTGGCTCTTTTAACCGGGGGAGTATCGGCTGCCAGGAA
GGTCAGGGTCATGGTTGACTTTAACTACCTCAATGAGGACAACCTGACAGAGTTAAAAAAACATGTAGTTAAGGTAAATT
ATAAGTTTCCGGAAATCAATGTAGTTGCCCTGACGGTAAAGGATAATGATATCCCTGAGATTAAGTCTTTGCCCGGTGTC
AAAGGAGTTTACCGTGATAATCAGGTTAAAGTTCATGGGGGACTTGTTTCCTGGAATCTCGATGTAATTGATGTTGAAAA
GGTGCATACTGATCATATTAATGAAGCAGATGGTTCTGGGGTTTATGTAGCTGTTCTCGATACCGGTCTTTTACCTAACT
GGAAGGATTATTTTCCGGAAGAAAAAATAGCATCTGAATATGCGGCAGGATTCCTCAATCCCAATGGTAATCGTAATCCC
GGGGCCTGGACAGGGACCCATGCCCATGGAACCCATGTAGCCAGTACCATTATTGGTTACTATCTATACGGGACACCGGT
TGATGGTGTGGCTCCGGGAGCGAAAATTATTCCCGTTAAAGTACTCAATAATAACGGTTATGGCTATGATTCTGCGGTTA
CAGCCGGCATCCTGTATGTTGCCAGCTTAAAGGCCAGTGGTAAAATTGTGGAGCCTGTTGTTATCAACATGAGCCTCGGG
AGTAGTGTCCCCAGTGCTCCGGAATTGAAGGCCATCAGGTATGCTATAGACCACGGGGTTATTGTTGTGGCTTCAGCCGG
GAATGAAGGCGAGGCCGGAATGGGATACCCCGGAGGTTATGATGAAGTAATTTCATGTGGGGCAGTAGGCTGGAAAAATA
TGTGGGTCAAAGGTTGGACCGGTGATGTTCCTGAAGAGGATATAGCATCCCGGGTATTTGTAGCTAATTTTAGTAGCCGG
GAACTTCCGGGCCAGCATCTGGATATTCTGGCTCCGGGGGTCTGGGTAATCGGACCTTATACCCTTTATGGAGCAGCCCA
TCCCCCCTACTGGGCTAAACAGGATAAGATGGGCCAGTATTATTATCTAAGTGGAACAAGTATGGCGGCCCCTCATGTAG
CAGGGACCGCGGCTCTGTTACTGGAGAAAAACCCTGCCCTGACCCAGCGGGAGGTTGAAGAGATACTGAAGGAAACAGCC
ACTTACATCCCACCGGCCAGTATATTTGTTCCCGATCCTGCCGGAGGTCAGACCTATACCTGGGGTAGTGATGCCACAGG
TTCTGGTTTGATAGATGTAGATGCGGCCCTGGAGGAGGCAGCTGGATACTAG

Upstream 100 bases:

>100_bases
GAATTAATTCTAGATATTTAGTGGTTATAACCTGGTTCTGTCAGGCATAACTATTTTAATGGAATAACAACTTCATCAAA
AAAAGAAAGGAGTAGTGAGC

Downstream 100 bases:

>100_bases
AAAATAATTTAGCTTCCTTATACCCCGGCAGAGTATTCTGCCGGGTTCTCTCTTTTATCAATATAGACTTCATTTTTTAA
TGGTTTGAGGGTAGGGAGGG

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MKKFLTLTLVTLLAVALLTGGVSAARKVRVMVDFNYLNEDNLTELKKHVVKVNYKFPEINVVALTVKDNDIPEIKSLPGV
KGVYRDNQVKVHGGLVSWNLDVIDVEKVHTDHINEADGSGVYVAVLDTGLLPNWKDYFPEEKIASEYAAGFLNPNGNRNP
GAWTGTHAHGTHVASTIIGYYLYGTPVDGVAPGAKIIPVKVLNNNGYGYDSAVTAGILYVASLKASGKIVEPVVINMSLG
SSVPSAPELKAIRYAIDHGVIVVASAGNEGEAGMGYPGGYDEVISCGAVGWKNMWVKGWTGDVPEEDIASRVFVANFSSR
ELPGQHLDILAPGVWVIGPYTLYGAAHPPYWAKQDKMGQYYYLSGTSMAAPHVAGTAALLLEKNPALTQREVEEILKETA
TYIPPASIFVPDPAGGQTYTWGSDATGSGLIDVDAALEEAAGY

Sequences:

>Translated_443_residues
MKKFLTLTLVTLLAVALLTGGVSAARKVRVMVDFNYLNEDNLTELKKHVVKVNYKFPEINVVALTVKDNDIPEIKSLPGV
KGVYRDNQVKVHGGLVSWNLDVIDVEKVHTDHINEADGSGVYVAVLDTGLLPNWKDYFPEEKIASEYAAGFLNPNGNRNP
GAWTGTHAHGTHVASTIIGYYLYGTPVDGVAPGAKIIPVKVLNNNGYGYDSAVTAGILYVASLKASGKIVEPVVINMSLG
SSVPSAPELKAIRYAIDHGVIVVASAGNEGEAGMGYPGGYDEVISCGAVGWKNMWVKGWTGDVPEEDIASRVFVANFSSR
ELPGQHLDILAPGVWVIGPYTLYGAAHPPYWAKQDKMGQYYYLSGTSMAAPHVAGTAALLLEKNPALTQREVEEILKETA
TYIPPASIFVPDPAGGQTYTWGSDATGSGLIDVDAALEEAAGY
>Mature_443_residues
MKKFLTLTLVTLLAVALLTGGVSAARKVRVMVDFNYLNEDNLTELKKHVVKVNYKFPEINVVALTVKDNDIPEIKSLPGV
KGVYRDNQVKVHGGLVSWNLDVIDVEKVHTDHINEADGSGVYVAVLDTGLLPNWKDYFPEEKIASEYAAGFLNPNGNRNP
GAWTGTHAHGTHVASTIIGYYLYGTPVDGVAPGAKIIPVKVLNNNGYGYDSAVTAGILYVASLKASGKIVEPVVINMSLG
SSVPSAPELKAIRYAIDHGVIVVASAGNEGEAGMGYPGGYDEVISCGAVGWKNMWVKGWTGDVPEEDIASRVFVANFSSR
ELPGQHLDILAPGVWVIGPYTLYGAAHPPYWAKQDKMGQYYYLSGTSMAAPHVAGTAALLLEKNPALTQREVEEILKETA
TYIPPASIFVPDPAGGQTYTWGSDATGSGLIDVDAALEEAAGY

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324576, Length=339, Percent_Identity=30.3834808259587, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320775, Length=290, Percent_Identity=28.2758620689655, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319893, Length=284, Percent_Identity=28.5211267605634, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 47349; Mature: 47349

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00136 SUBTILASE_ASP ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFLTLTLVTLLAVALLTGGVSAARKVRVMVDFNYLNEDNLTELKKHVVKVNYKFPEIN
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHEEEEEEECCCEE
VVALTVKDNDIPEIKSLPGVKGVYRDNQVKVHGGLVSWNLDVIDVEKVHTDHINEADGSG
EEEEEEECCCCCHHHHCCCCCCEECCCEEEEECCEEEEEEEEEEEEHHCCCCCCCCCCCE
VYVAVLDTGLLPNWKDYFPEEKIASEYAAGFLNPNGNRNPGAWTGTHAHGTHVASTIIGY
EEEEEEECCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YLYGTPVDGVAPGAKIIPVKVLNNNGYGYDSAVTAGILYVASLKASGKIVEPVVINMSLG
EEECCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHEEEEECCCCCEECEEEEEEECC
SSVPSAPELKAIRYAIDHGVIVVASAGNEGEAGMGYPGGYDEVISCGAVGWKNMWVKGWT
CCCCCCCCHHHHEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCC
GDVPEEDIASRVFVANFSSRELPGQHLDILAPGVWVIGPYTLYGAAHPPYWAKQDKMGQY
CCCCHHHHHCEEEEEECCCCCCCCCCEEEECCCEEEECCEEEECCCCCCCCCCCCCCCCE
YYLSGTSMAAPHVAGTAALLLEKNPALTQREVEEILKETATYIPPASIFVPDPAGGQTYT
EEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEE
WGSDATGSGLIDVDAALEEAAGY
CCCCCCCCCEEEHHHHHHHHCCC
>Mature Secondary Structure
MKKFLTLTLVTLLAVALLTGGVSAARKVRVMVDFNYLNEDNLTELKKHVVKVNYKFPEIN
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHEEEEEEECCCEE
VVALTVKDNDIPEIKSLPGVKGVYRDNQVKVHGGLVSWNLDVIDVEKVHTDHINEADGSG
EEEEEEECCCCCHHHHCCCCCCEECCCEEEEECCEEEEEEEEEEEEHHCCCCCCCCCCCE
VYVAVLDTGLLPNWKDYFPEEKIASEYAAGFLNPNGNRNPGAWTGTHAHGTHVASTIIGY
EEEEEEECCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YLYGTPVDGVAPGAKIIPVKVLNNNGYGYDSAVTAGILYVASLKASGKIVEPVVINMSLG
EEECCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHEEEEECCCCCEECEEEEEEECC
SSVPSAPELKAIRYAIDHGVIVVASAGNEGEAGMGYPGGYDEVISCGAVGWKNMWVKGWT
CCCCCCCCHHHHEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCC
GDVPEEDIASRVFVANFSSRELPGQHLDILAPGVWVIGPYTLYGAAHPPYWAKQDKMGQY
CCCCHHHHHCEEEEEECCCCCCCCCCEEEECCCEEEECCEEEECCCCCCCCCCCCCCCCE
YYLSGTSMAAPHVAGTAALLLEKNPALTQREVEEILKETATYIPPASIFVPDPAGGQTYT
EEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEE
WGSDATGSGLIDVDAALEEAAGY
CCCCCCCCCEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]