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The map label for this gene is amyS [H]

Identifier: 220932660

GI number: 220932660

Start: 1947407

End: 1949278

Strand: Reverse

Name: amyS [H]

Synonym: Hore_18240

Alternate gene names: 220932660

Gene position: 1949278-1947407 (Counterclockwise)

Preceding gene: 220932661

Following gene: 220932659

Centisome position: 75.61

GC content: 42.2

Gene sequence:

>1872_bases
GTGGTCTTTATTAAATTATTTATAAAATACAAAATATATGCGGCCTTAATACTATTAATACTATTACTATCAGGATGTTC
TAACATTAGCGAAGATGTCAATAATCCTAACCGGAGTTTATTTTTAATCGAATCTGAACCATCTACAGGTGCCAGTGTAT
CTAAAAACTTAACTGAAATCATCCTGATATTCAGTAATGACATAAATAAGGTTTCCCAGCTGGCCTTAACAGATTTAATA
ACTGATAGTGATATTCAGGGAATTGACTATAATATAGAAGGAAACAAAGTAATTATCAATAACTTTTCCCTGGAACCAAC
CTGTAATTACCGGTTATCATATGAGGTTATAGATATTTATGATAATCATTTACAGGGTTATATAGAGTTTTTGGTTAACC
AGAGTAATTATCCTCAGATACCGGACCAGGAGGTTAACCACACAATCCTTCAGGCATTCTACTGGGAGATGAATACCGGT
GAATATGCCACTGAACACCCTGAAGAAGCAAACCTCTGGAACCTACTGGCTGAGAGGGCTCCTGAACTGGCGGAGGCAGG
GTTTACTGCAGTCTGGCTGCCGCCGGCCAATAAGGGTATGGCAGGCATACATGATGTGGGTTATGGCACCTATGACCTCT
GGGATCTGGGAGAATTTGACCAGAAGGGGACGGTAAGGACCAAGTATGGAACAAAGGGCGAGCTGGAAAATGCCATTGAT
GCTCTGCATAACAATGACATTAAGGTTTATTTTGATGCTGTCTTAAACCACAGGATGGGGGCTGATTATGCTGAAACGGT
CCTCCTTGATGAGAATAGTCGAGATAAACCGGGTCAGTATATCAAGGCCTGGACAGGTTTTAATTTCCCCGGGCGTAATG
GTGAATACAGTAATTTTACATGGAACGGGCAGTGTTTTGATGGAACAGACTGGGATGATTATAGTAAAGAATCTGGTAAG
TATTTATTTGACGAAAAGAGCTGGGACTGGACCTATAACTGGGATGAAGACTACCTCATGGGGGCCGATGTTGATTATGA
AAATGAAGCTGTCCAGAATGATGTAATAGACTGGGGTCAGTGGATTATTAACAACATTGATTTTGATGGTTTCAGGCTTG
ATGCCGTTAAGCATATTGACTATAGATTTATTGACAAATGGATGAGTGCGGTTCAGAACAGTTCAAACAGGGATGTCTTC
TTTGTCGGTGAGGCCTGGGTTGAAGATGTTGATGACCTGAAAGGTTTCCTGGATACAGTCGGTAATCCCGACCTCAGGGT
CTTTGATTTTCCATTGAGGTCTTTCTTTGTTGACATGCTGAATGGTGCCTATATGGCTGACCTGAGGAATGCTGGCCTTG
TTAATAGCCCCGGGTATGAGAACAGGGCAGTCACCTTTGTCGATAACCATGATACTGATCGGGATGAGGGGAGTTATACA
GTTTCAATCTACAGTCGTAAATACCAGGCCTATGCCTATATCCTGACCAGGGCTGAAGGTGTTCCTACTGTTTACTGGAA
GGATTATTATATCTGGGAAATGAAAGAAGGACTGGATAAGCTCTTAACGGCAAGACGCTATTATGCCTATGGCCCCGGTT
ATGAGGTGGATAACAATGATGCCGATATCTATTCCTATGTCCGCAGTGGTTTCCCTGATGTAGCTGGTGATGGTCTTGTG
CTAATGATATCTGATGGAACTTCAGGGAATGTTGCAGGCAAATGGATAAATTCCCGGCAACCGGATACAGAGTTTTATGA
CCTGACAGGCCACATCAAGGAACATGTTACTACTGACAGTGAAGGTTATGGAAACTTCAAGGTAATCAAAAGTGAAGATA
AAGGCTGGTCCATCTGGGTTCCGGTGGAGTAG

Upstream 100 bases:

>100_bases
CACCCTTAAATAAATAATGTAAGTTTAATAGATAAACCCCCTGGGTAAAAATCCGGGGGGTTTTTAAATATTACGGTAAT
AACATCTGGATGCGAAAGGA

Downstream 100 bases:

>100_bases
GGAATGGTCGGGGTGTTAACCCCGGCTTTTTTTAAAACTCAGGATGCTTGACACCTGAAATTTTCCGGTGATATAATGTA
ATGTAAAGGATTACATACAT

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase; BLA [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MVFIKLFIKYKIYAALILLILLLSGCSNISEDVNNPNRSLFLIESEPSTGASVSKNLTEIILIFSNDINKVSQLALTDLI
TDSDIQGIDYNIEGNKVIINNFSLEPTCNYRLSYEVIDIYDNHLQGYIEFLVNQSNYPQIPDQEVNHTILQAFYWEMNTG
EYATEHPEEANLWNLLAERAPELAEAGFTAVWLPPANKGMAGIHDVGYGTYDLWDLGEFDQKGTVRTKYGTKGELENAID
ALHNNDIKVYFDAVLNHRMGADYAETVLLDENSRDKPGQYIKAWTGFNFPGRNGEYSNFTWNGQCFDGTDWDDYSKESGK
YLFDEKSWDWTYNWDEDYLMGADVDYENEAVQNDVIDWGQWIINNIDFDGFRLDAVKHIDYRFIDKWMSAVQNSSNRDVF
FVGEAWVEDVDDLKGFLDTVGNPDLRVFDFPLRSFFVDMLNGAYMADLRNAGLVNSPGYENRAVTFVDNHDTDRDEGSYT
VSIYSRKYQAYAYILTRAEGVPTVYWKDYYIWEMKEGLDKLLTARRYYAYGPGYEVDNNDADIYSYVRSGFPDVAGDGLV
LMISDGTSGNVAGKWINSRQPDTEFYDLTGHIKEHVTTDSEGYGNFKVIKSEDKGWSIWVPVE

Sequences:

>Translated_623_residues
MVFIKLFIKYKIYAALILLILLLSGCSNISEDVNNPNRSLFLIESEPSTGASVSKNLTEIILIFSNDINKVSQLALTDLI
TDSDIQGIDYNIEGNKVIINNFSLEPTCNYRLSYEVIDIYDNHLQGYIEFLVNQSNYPQIPDQEVNHTILQAFYWEMNTG
EYATEHPEEANLWNLLAERAPELAEAGFTAVWLPPANKGMAGIHDVGYGTYDLWDLGEFDQKGTVRTKYGTKGELENAID
ALHNNDIKVYFDAVLNHRMGADYAETVLLDENSRDKPGQYIKAWTGFNFPGRNGEYSNFTWNGQCFDGTDWDDYSKESGK
YLFDEKSWDWTYNWDEDYLMGADVDYENEAVQNDVIDWGQWIINNIDFDGFRLDAVKHIDYRFIDKWMSAVQNSSNRDVF
FVGEAWVEDVDDLKGFLDTVGNPDLRVFDFPLRSFFVDMLNGAYMADLRNAGLVNSPGYENRAVTFVDNHDTDRDEGSYT
VSIYSRKYQAYAYILTRAEGVPTVYWKDYYIWEMKEGLDKLLTARRYYAYGPGYEVDNNDADIYSYVRSGFPDVAGDGLV
LMISDGTSGNVAGKWINSRQPDTEFYDLTGHIKEHVTTDSEGYGNFKVIKSEDKGWSIWVPVE
>Mature_623_residues
MVFIKLFIKYKIYAALILLILLLSGCSNISEDVNNPNRSLFLIESEPSTGASVSKNLTEIILIFSNDINKVSQLALTDLI
TDSDIQGIDYNIEGNKVIINNFSLEPTCNYRLSYEVIDIYDNHLQGYIEFLVNQSNYPQIPDQEVNHTILQAFYWEMNTG
EYATEHPEEANLWNLLAERAPELAEAGFTAVWLPPANKGMAGIHDVGYGTYDLWDLGEFDQKGTVRTKYGTKGELENAID
ALHNNDIKVYFDAVLNHRMGADYAETVLLDENSRDKPGQYIKAWTGFNFPGRNGEYSNFTWNGQCFDGTDWDDYSKESGK
YLFDEKSWDWTYNWDEDYLMGADVDYENEAVQNDVIDWGQWIINNIDFDGFRLDAVKHIDYRFIDKWMSAVQNSSNRDVF
FVGEAWVEDVDDLKGFLDTVGNPDLRVFDFPLRSFFVDMLNGAYMADLRNAGLVNSPGYENRAVTFVDNHDTDRDEGSYT
VSIYSRKYQAYAYILTRAEGVPTVYWKDYYIWEMKEGLDKLLTARRYYAYGPGYEVDNNDADIYSYVRSGFPDVAGDGLV
LMISDGTSGNVAGKWINSRQPDTEFYDLTGHIKEHVTTDSEGYGNFKVIKSEDKGWSIWVPVE

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=496, Percent_Identity=35.6854838709677, Blast_Score=261, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 71348; Mature: 71348

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFIKLFIKYKIYAALILLILLLSGCSNISEDVNNPNRSLFLIESEPSTGASVSKNLTEI
CEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCCCCCCHHHCCCEEE
ILIFSNDINKVSQLALTDLITDSDIQGIDYNIEGNKVIINNFSLEPTCNYRLSYEVIDIY
EEEEECCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCCCCEEEEEEEEEEH
DNHLQGYIEFLVNQSNYPQIPDQEVNHTILQAFYWEMNTGEYATEHPEEANLWNLLAERA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHC
PELAEAGFTAVWLPPANKGMAGIHDVGYGTYDLWDLGEFDQKGTVRTKYGTKGELENAID
CHHHHCCCEEEEECCCCCCCCCHHHCCCCCEECCCCCCCCCCCCEEECCCCCCHHHHHHH
ALHNNDIKVYFDAVLNHRMGADYAETVLLDENSRDKPGQYIKAWTGFNFPGRNGEYSNFT
HHCCCCEEEEEEHHHHHCCCCCHHHEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCEE
WNGQCFDGTDWDDYSKESGKYLFDEKSWDWTYNWDEDYLMGADVDYENEAVQNDVIDWGQ
ECCEEECCCCCCCCCCCCCCEEECCCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHH
WIINNIDFDGFRLDAVKHIDYRFIDKWMSAVQNSSNRDVFFVGEAWVEDVDDLKGFLDTV
HHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHC
GNPDLRVFDFPLRSFFVDMLNGAYMADLRNAGLVNSPGYENRAVTFVDNHDTDRDEGSYT
CCCCEEEEECCHHHHHHHHHCCCEEEHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEE
VSIYSRKYQAYAYILTRAEGVPTVYWKDYYIWEMKEGLDKLLTARRYYAYGPGYEVDNND
EEEEECCEEEEEEEEEECCCCCEEEECCEEEEEHHHHHHHHHHHHHHEEECCCCCCCCCC
ADIYSYVRSGFPDVAGDGLVLMISDGTSGNVAGKWINSRQPDTEFYDLTGHIKEHVTTDS
HHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCEEECHHHHHHHCCCCC
EGYGNFKVIKSEDKGWSIWVPVE
CCCCCEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MVFIKLFIKYKIYAALILLILLLSGCSNISEDVNNPNRSLFLIESEPSTGASVSKNLTEI
CEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCCCCCCHHHCCCEEE
ILIFSNDINKVSQLALTDLITDSDIQGIDYNIEGNKVIINNFSLEPTCNYRLSYEVIDIY
EEEEECCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCCCCEEEEEEEEEEH
DNHLQGYIEFLVNQSNYPQIPDQEVNHTILQAFYWEMNTGEYATEHPEEANLWNLLAERA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHC
PELAEAGFTAVWLPPANKGMAGIHDVGYGTYDLWDLGEFDQKGTVRTKYGTKGELENAID
CHHHHCCCEEEEECCCCCCCCCHHHCCCCCEECCCCCCCCCCCCEEECCCCCCHHHHHHH
ALHNNDIKVYFDAVLNHRMGADYAETVLLDENSRDKPGQYIKAWTGFNFPGRNGEYSNFT
HHCCCCEEEEEEHHHHHCCCCCHHHEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCEE
WNGQCFDGTDWDDYSKESGKYLFDEKSWDWTYNWDEDYLMGADVDYENEAVQNDVIDWGQ
ECCEEECCCCCCCCCCCCCCEEECCCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHH
WIINNIDFDGFRLDAVKHIDYRFIDKWMSAVQNSSNRDVFFVGEAWVEDVDDLKGFLDTV
HHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHC
GNPDLRVFDFPLRSFFVDMLNGAYMADLRNAGLVNSPGYENRAVTFVDNHDTDRDEGSYT
CCCCEEEEECCHHHHHHHHHCCCEEEHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEE
VSIYSRKYQAYAYILTRAEGVPTVYWKDYYIWEMKEGLDKLLTARRYYAYGPGYEVDNND
EEEEECCEEEEEEEEEECCCCCEEEECCEEEEEHHHHHHHHHHHHHHEEECCCCCCCCCC
ADIYSYVRSGFPDVAGDGLVLMISDGTSGNVAGKWINSRQPDTEFYDLTGHIKEHVTTDS
HHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCEEECHHHHHHHCCCCC
EGYGNFKVIKSEDKGWSIWVPVE
CCCCCEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2418011; 3009417; 14632998; 6334606; 6609154; 2265757; 2540150; 6172418; 11997021; 2394736; 8771184; 10966804; 12736372; 12915728; 7877175; 9551551; 10924103; 12540849 [H]