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The map label for this gene is ahpF [H]

Identifier: 220932649

GI number: 220932649

Start: 1934758

End: 1935402

Strand: Reverse

Name: ahpF [H]

Synonym: Hore_18130

Alternate gene names: 220932649

Gene position: 1935402-1934758 (Counterclockwise)

Preceding gene: 220932650

Following gene: 220932648

Centisome position: 75.07

GC content: 39.84

Gene sequence:

>645_bases
ATGGCCAAAATCGGTAGTGAAGACAAAGAGAGAATTAAAGAGTTATTCAGTGAGAACCTTGAGGGAGATGTTAATATTCA
TTTCTTTACAGATGGTGATGCCTGTGAGTACTGTGAAGATACCAGGGAAATTTTGGATGAAATCTCATCACTGGATGACA
GAATTAATCTGACAGTTAACAATCTTGGAGAAGAAGAAGCTGAGAGGCTGGGAATAAATAGGGCACCGGCTATTGTATTC
GTGGATGATGAGGGGGAAGATTTAGGTGTTCATTTTTATGGTATCCCCTCCGGTTATGAGTTTAACACCTTAATAGAGGA
TATTATTGACATTTCAAATAATGGCCCCCGGGACCTTGGAGATGATATTAAAGAACAAATCAAAGCTATTGATAAAGATG
TTCGTCTTCAGGTATTTATTACTCCGACCTGTCCCTATTGCCCCAGGGCTGTCAGGGTTGCTCACCAGATGGCTATGCTT
AACCCCAAAGTCAGGGGTGAGATGATTGAAGCTATTGAATTCAGGGACTTATCAGAAAAATATAATGTATCCGGGGTTCC
TAAAACAGTTATTAATGATGGTGCTGCAGAACAGGTTGGAGCTGTTCCGGCCCAGGTTATTTTAAATAAAATAAAAGAAG
TTTAA

Upstream 100 bases:

>100_bases
TTTATTTAAATTTAAGGGTTTGTAAATTATTTAACATTAAAATTATTGATATGGTTTATAATATGAAGTGAATAGAAAGC
ATAAGGAGAGTGAAATAAAG

Downstream 100 bases:

>100_bases
ATCTGATTATACAATTACAGGTTATTTTTTGACTGGCATTTGTTCATATTTGCGCAATGTCAGGTAGTAAAGACCATTTT
TAATAGAGATAATAAAAATC

Product: glutaredoxin-like domain-containing protein

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MAKIGSEDKERIKELFSENLEGDVNIHFFTDGDACEYCEDTREILDEISSLDDRINLTVNNLGEEEAERLGINRAPAIVF
VDDEGEDLGVHFYGIPSGYEFNTLIEDIIDISNNGPRDLGDDIKEQIKAIDKDVRLQVFITPTCPYCPRAVRVAHQMAML
NPKVRGEMIEAIEFRDLSEKYNVSGVPKTVINDGAAEQVGAVPAQVILNKIKEV

Sequences:

>Translated_214_residues
MAKIGSEDKERIKELFSENLEGDVNIHFFTDGDACEYCEDTREILDEISSLDDRINLTVNNLGEEEAERLGINRAPAIVF
VDDEGEDLGVHFYGIPSGYEFNTLIEDIIDISNNGPRDLGDDIKEQIKAIDKDVRLQVFITPTCPYCPRAVRVAHQMAML
NPKVRGEMIEAIEFRDLSEKYNVSGVPKTVINDGAAEQVGAVPAQVILNKIKEV
>Mature_213_residues
AKIGSEDKERIKELFSENLEGDVNIHFFTDGDACEYCEDTREILDEISSLDDRINLTVNNLGEEEAERLGINRAPAIVFV
DDEGEDLGVHFYGIPSGYEFNTLIEDIIDISNNGPRDLGDDIKEQIKAIDKDVRLQVFITPTCPYCPRAVRVAHQMAMLN
PKVRGEMIEAIEFRDLSEKYNVSGVPKTVINDGAAEQVGAVPAQVILNKIKEV

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=151, Percent_Identity=27.8145695364238, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 23901; Mature: 23770

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: PS00195 GLUTAREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIGSEDKERIKELFSENLEGDVNIHFFTDGDACEYCEDTREILDEISSLDDRINLTVN
CCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHEEEEEC
NLGEEEAERLGINRAPAIVFVDDEGEDLGVHFYGIPSGYEFNTLIEDIIDISNNGPRDLG
CCCHHHHHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHH
DDIKEQIKAIDKDVRLQVFITPTCPYCPRAVRVAHQMAMLNPKVRGEMIEAIEFRDLSEK
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YNVSGVPKTVINDGAAEQVGAVPAQVILNKIKEV
CCCCCCCHHHHCCCCHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKIGSEDKERIKELFSENLEGDVNIHFFTDGDACEYCEDTREILDEISSLDDRINLTVN
CCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHEEEEEC
NLGEEEAERLGINRAPAIVFVDDEGEDLGVHFYGIPSGYEFNTLIEDIIDISNNGPRDLG
CCCHHHHHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHH
DDIKEQIKAIDKDVRLQVFITPTCPYCPRAVRVAHQMAMLNPKVRGEMIEAIEFRDLSEK
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YNVSGVPKTVINDGAAEQVGAVPAQVILNKIKEV
CCCCCCCHHHHCCCCHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA