Click here to switch to the map view.

The map label for this gene is prfA [H]

Identifier: 220932633

GI number: 220932633

Start: 1921607

End: 1922683

Strand: Reverse

Name: prfA [H]

Synonym: Hore_17970

Alternate gene names: 220932633

Gene position: 1922683-1921607 (Counterclockwise)

Preceding gene: 220932634

Following gene: 220932632

Centisome position: 74.58

GC content: 42.9

Gene sequence:

>1077_bases
ATGTTTGATTTAACGAAAAAACTTGATAAATTAGTCGATAGATATAATGAACTTAACAAAGAAATCAGTGACCCTGATGT
TATTAATAACAGTGAACAGTATCAAAAACTTCTCAAAGAACATGGTAAATTAAAAGAGATTGTCAGCAAATACAGGGAGT
ACAAAAAGGTGCTAAGCCGGGTTGAAGAAGCCCACTCCATTATTGAAGAGGAAGAGGAGCAGGAAGTTAGAGAACTGGCT
GAAATGGAGCTTAAGGAATTAACCCCGCGCAAGGAGAAAATGGAAGATGAGCTTACCTATATGTTGCTACCTGAAGACCC
CAATGATGAAAAAAATGTTATTGTTGAAATCAGGGCCGGGGCCGGAGGAGACGAAGCCGGCCTTTTTGCTGCTGACCTTT
ACCGGATGTATTCCCGGTATGCTGAAAGCAAAAACTGGAAAACAGAAGTTATAAGTGCCAATGCTTCTGGAATAGGCGGG
TATAAGGAGATTATATTTACGGTTGAAGGAAAAAATGTATACAGCCGTCTTAAATATGAAAGTGGGGTGCACCGGGTTCA
GAGGGTTCCGACCACTGAATCTGGAGGCCGGATTCATACATCTACAGCCACTGTTGCCGTTCTACCTGAGGCTGAAGAGG
TTGATGTAGATATTGACCCCAATGATTTACGGATAGATGTTTACAGGTCAAGTGGCCCCGGAGGGCAGAGTGTTAATACT
ACTGACTCTGCCGTGCGGATAACCCATGAACCGACAGGGCTGGTTGTATCCTGTCAGGATGAAAAATCACAGCATAAGAA
CAAAGCCAGGGCCATGCGTATTCTACGGGCACGGCTCAAGGAGTTAATGGAAAAAGAAAAGCAGGCGGAAATTGATGAAG
CCCGGAAAAGCCAGATCGGCTCCGGGGACCGCAGTGAGAAAATCAGGACCTATAATTTCCCCCAGGGACGGGTCAGTGAC
CACAGAATCAATTTGACCATTCATCAATTGAATGATATTCTGGACGGAAACCTGGATCTTATTATTGATAATTTAATTAA
AGAAGATACTATTAAAAGATTGGAGAAGCTAAAATAG

Upstream 100 bases:

>100_bases
CTGCAAAAATTAACGACAGTCGAACCAGATGATTCTATGCTAGAGGTTGCTATCGCTGCCCTTAAGGCAGTATTACCAGA
AGAAGAAAGGAATGGGTCAA

Downstream 100 bases:

>100_bases
TGACTGTAAAAGAGGTATTAAACAGTGCTATTGATTTTTTTAAAAAGCAGAATATAGATAATCCCCGGCTTGATGCTGAA
GTCCTTCTGGCTCATCTCCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MFDLTKKLDKLVDRYNELNKEISDPDVINNSEQYQKLLKEHGKLKEIVSKYREYKKVLSRVEEAHSIIEEEEEQEVRELA
EMELKELTPRKEKMEDELTYMLLPEDPNDEKNVIVEIRAGAGGDEAGLFAADLYRMYSRYAESKNWKTEVISANASGIGG
YKEIIFTVEGKNVYSRLKYESGVHRVQRVPTTESGGRIHTSTATVAVLPEAEEVDVDIDPNDLRIDVYRSSGPGGQSVNT
TDSAVRITHEPTGLVVSCQDEKSQHKNKARAMRILRARLKELMEKEKQAEIDEARKSQIGSGDRSEKIRTYNFPQGRVSD
HRINLTIHQLNDILDGNLDLIIDNLIKEDTIKRLEKLK

Sequences:

>Translated_358_residues
MFDLTKKLDKLVDRYNELNKEISDPDVINNSEQYQKLLKEHGKLKEIVSKYREYKKVLSRVEEAHSIIEEEEEQEVRELA
EMELKELTPRKEKMEDELTYMLLPEDPNDEKNVIVEIRAGAGGDEAGLFAADLYRMYSRYAESKNWKTEVISANASGIGG
YKEIIFTVEGKNVYSRLKYESGVHRVQRVPTTESGGRIHTSTATVAVLPEAEEVDVDIDPNDLRIDVYRSSGPGGQSVNT
TDSAVRITHEPTGLVVSCQDEKSQHKNKARAMRILRARLKELMEKEKQAEIDEARKSQIGSGDRSEKIRTYNFPQGRVSD
HRINLTIHQLNDILDGNLDLIIDNLIKEDTIKRLEKLK
>Mature_358_residues
MFDLTKKLDKLVDRYNELNKEISDPDVINNSEQYQKLLKEHGKLKEIVSKYREYKKVLSRVEEAHSIIEEEEEQEVRELA
EMELKELTPRKEKMEDELTYMLLPEDPNDEKNVIVEIRAGAGGDEAGLFAADLYRMYSRYAESKNWKTEVISANASGIGG
YKEIIFTVEGKNVYSRLKYESGVHRVQRVPTTESGGRIHTSTATVAVLPEAEEVDVDIDPNDLRIDVYRSSGPGGQSVNT
TDSAVRITHEPTGLVVSCQDEKSQHKNKARAMRILRARLKELMEKEKQAEIDEARKSQIGSGDRSEKIRTYNFPQGRVSD
HRINLTIHQLNDILDGNLDLIIDNLIKEDTIKRLEKLK

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=48.014440433213, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI34577120, Length=355, Percent_Identity=40.2816901408451, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI166795305, Length=182, Percent_Identity=45.6043956043956, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=50.561797752809, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=39.6449704142012, Blast_Score=228, Evalue=7e-61,
Organism=Caenorhabditis elegans, GI17542784, Length=309, Percent_Identity=38.8349514563107, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=44.9848024316109, Blast_Score=262, Evalue=6e-71,
Organism=Drosophila melanogaster, GI19921226, Length=336, Percent_Identity=41.9642857142857, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40961; Mature: 40961

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDLTKKLDKLVDRYNELNKEISDPDVINNSEQYQKLLKEHGKLKEIVSKYREYKKVLSR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VEEAHSIIEEEEEQEVRELAEMELKELTPRKEKMEDELTYMLLPEDPNDEKNVIVEIRAG
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCCCEEEEEECC
AGGDEAGLFAADLYRMYSRYAESKNWKTEVISANASGIGGYKEIIFTVEGKNVYSRLKYE
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHH
SGVHRVQRVPTTESGGRIHTSTATVAVLPEAEEVDVDIDPNDLRIDVYRSSGPGGQSVNT
HHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEECCCCCEEEEEEECCCCCCCCCCC
TDSAVRITHEPTGLVVSCQDEKSQHKNKARAMRILRARLKELMEKEKQAEIDEARKSQIG
CCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGDRSEKIRTYNFPQGRVSDHRINLTIHQLNDILDGNLDLIIDNLIKEDTIKRLEKLK
CCCCCCCCEEECCCCCCCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFDLTKKLDKLVDRYNELNKEISDPDVINNSEQYQKLLKEHGKLKEIVSKYREYKKVLSR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VEEAHSIIEEEEEQEVRELAEMELKELTPRKEKMEDELTYMLLPEDPNDEKNVIVEIRAG
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCCCEEEEEECC
AGGDEAGLFAADLYRMYSRYAESKNWKTEVISANASGIGGYKEIIFTVEGKNVYSRLKYE
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHH
SGVHRVQRVPTTESGGRIHTSTATVAVLPEAEEVDVDIDPNDLRIDVYRSSGPGGQSVNT
HHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEECCCCCEEEEEEECCCCCCCCCCC
TDSAVRITHEPTGLVVSCQDEKSQHKNKARAMRILRARLKELMEKEKQAEIDEARKSQIG
CCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGDRSEKIRTYNFPQGRVSDHRINLTIHQLNDILDGNLDLIIDNLIKEDTIKRLEKLK
CCCCCCCCEEECCCCCCCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA