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The map label for this gene is hemK [H]

Identifier: 220932632

GI number: 220932632

Start: 1920750

End: 1921607

Strand: Reverse

Name: hemK [H]

Synonym: Hore_17960

Alternate gene names: 220932632

Gene position: 1921607-1920750 (Counterclockwise)

Preceding gene: 220932633

Following gene: 220932631

Centisome position: 74.53

GC content: 43.82

Gene sequence:

>858_bases
GTGACTGTAAAAGAGGTATTAAACAGTGCTATTGATTTTTTTAAAAAGCAGAATATAGATAATCCCCGGCTTGATGCTGA
AGTCCTTCTGGCTCATCTCCTTGATATGGAACGGATTCAGCTTTATGTTAAATATGATTTACCGCTAAAAAATAAAGAAG
TTGAAGCTTACCGGGAGATGGTTATTAACCGGGCCAGGGGGATACCTGTTGCCTATCTAACAGGACACAAAGAATTTATG
TCCCTTGATTTTAAAGTGAACAGGAGTGTTTTAATACCCAGACCGGAGACCGAGATTCTGGTGGAAGAGATAATTTCACT
CTGTCAGGCAAAAAATATTGATAATCCCAATATCGTTGATGTAGGAACGGGGAGCGGGGTTATAGCTGTCAGCCTTGCTC
ACTATCTCCCCGGAGCCCGGGTTCTGGGGATTGATATATCCGATAAGGCTCTGGAAGTGGCCCGGACCAACATCAAGAGG
CATAATCTAGGGGAGAGGGTCAAGGTTATTAAGGGTAATTTACTTGATCCTTTAATTAAAATGGAAAAGGATAATGTTAA
TATAGTTGTCTCCAATCCCCCTTACCTGACCGGAAATGAAATGAAAAAACTGCCGCTGGAAGTTACCTATGAGCCTTCAC
AGGCCCTTGATGGAGGGGCTGATGGCTTAAAGATTTACCGGGAGTTAATCCCGCGGGCTTTGAAAGTACTTGTCCCGGGA
GGTATTTTAGGACTGGAGATAGGTTACCATCAGGCCGACAGTATCAGGGATATTTTGTCAGGCCTGCCCTGGGGTGATAT
CAGGGTTTTACAGGATTATTCTGGCCTGGACCGGGTTGTTATTGCCCGCAAAAAATAA

Upstream 100 bases:

>100_bases
CCATTCATCAATTGAATGATATTCTGGACGGAAACCTGGATCTTATTATTGATAATTTAATTAAAGAAGATACTATTAAA
AGATTGGAGAAGCTAAAATA

Downstream 100 bases:

>100_bases
AACAGGGAAGGAAATTGAGGATGGAATATAAAACCAGAATCGGCAGGGTCAATCGGGACCTTATTAAAAAACAGGATATA
CTTAAACTGAAAGAGAATGA

Product: modification methylase, HemK family

Products: NA

Alternate protein names: HemK protein homolog; M.RprHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTVKEVLNSAIDFFKKQNIDNPRLDAEVLLAHLLDMERIQLYVKYDLPLKNKEVEAYREMVINRARGIPVAYLTGHKEFM
SLDFKVNRSVLIPRPETEILVEEIISLCQAKNIDNPNIVDVGTGSGVIAVSLAHYLPGARVLGIDISDKALEVARTNIKR
HNLGERVKVIKGNLLDPLIKMEKDNVNIVVSNPPYLTGNEMKKLPLEVTYEPSQALDGGADGLKIYRELIPRALKVLVPG
GILGLEIGYHQADSIRDILSGLPWGDIRVLQDYSGLDRVVIARKK

Sequences:

>Translated_285_residues
MTVKEVLNSAIDFFKKQNIDNPRLDAEVLLAHLLDMERIQLYVKYDLPLKNKEVEAYREMVINRARGIPVAYLTGHKEFM
SLDFKVNRSVLIPRPETEILVEEIISLCQAKNIDNPNIVDVGTGSGVIAVSLAHYLPGARVLGIDISDKALEVARTNIKR
HNLGERVKVIKGNLLDPLIKMEKDNVNIVVSNPPYLTGNEMKKLPLEVTYEPSQALDGGADGLKIYRELIPRALKVLVPG
GILGLEIGYHQADSIRDILSGLPWGDIRVLQDYSGLDRVVIARKK
>Mature_284_residues
TVKEVLNSAIDFFKKQNIDNPRLDAEVLLAHLLDMERIQLYVKYDLPLKNKEVEAYREMVINRARGIPVAYLTGHKEFMS
LDFKVNRSVLIPRPETEILVEEIISLCQAKNIDNPNIVDVGTGSGVIAVSLAHYLPGARVLGIDISDKALEVARTNIKRH
NLGERVKVIKGNLLDPLIKMEKDNVNIVVSNPPYLTGNEMKKLPLEVTYEPSQALDGGADGLKIYRELIPRALKVLVPGG
ILGLEIGYHQADSIRDILSGLPWGDIRVLQDYSGLDRVVIARKK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=296, Percent_Identity=28.3783783783784, Blast_Score=121, Evalue=9e-28,
Organism=Escherichia coli, GI1787463, Length=286, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI87082085, Length=262, Percent_Identity=34.3511450381679, Blast_Score=123, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324266, Length=202, Percent_Identity=27.2277227722772, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582226, Length=266, Percent_Identity=29.3233082706767, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 31834; Mature: 31702

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKEVLNSAIDFFKKQNIDNPRLDAEVLLAHLLDMERIQLYVKYDLPLKNKEVEAYREM
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHEEEEEEECCCCCCCHHHHHHHH
VINRARGIPVAYLTGHKEFMSLDFKVNRSVLIPRPETEILVEEIISLCQAKNIDNPNIVD
HHHHHCCCEEEEEECCHHHEEEEEEECCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEE
VGTGSGVIAVSLAHYLPGARVLGIDISDKALEVARTNIKRHNLGERVKVIKGNLLDPLIK
ECCCCCHHEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHH
MEKDNVNIVVSNPPYLTGNEMKKLPLEVTYEPSQALDGGADGLKIYRELIPRALKVLVPG
CCCCCEEEEECCCCEECCCCHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHCCC
GILGLEIGYHQADSIRDILSGLPWGDIRVLQDYSGLDRVVIARKK
CEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECC
>Mature Secondary Structure 
TVKEVLNSAIDFFKKQNIDNPRLDAEVLLAHLLDMERIQLYVKYDLPLKNKEVEAYREM
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHEEEEEEECCCCCCCHHHHHHHH
VINRARGIPVAYLTGHKEFMSLDFKVNRSVLIPRPETEILVEEIISLCQAKNIDNPNIVD
HHHHHCCCEEEEEECCHHHEEEEEEECCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEE
VGTGSGVIAVSLAHYLPGARVLGIDISDKALEVARTNIKRHNLGERVKVIKGNLLDPLIK
ECCCCCHHEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHH
MEKDNVNIVVSNPPYLTGNEMKKLPLEVTYEPSQALDGGADGLKIYRELIPRALKVLVPG
CCCCCEEEEECCCCEECCCCHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHCCC
GILGLEIGYHQADSIRDILSGLPWGDIRVLQDYSGLDRVVIARKK
CEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]