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The map label for this gene is prfB [H]

Identifier: 220932479

GI number: 220932479

Start: 1765368

End: 1766363

Strand: Reverse

Name: prfB [H]

Synonym: Hore_16430

Alternate gene names: 220932479

Gene position: 1766363-1765368 (Counterclockwise)

Preceding gene: 220932480

Following gene: 220932478

Centisome position: 68.51

GC content: 44.08

Gene sequence:

>996_bases
ATGTCAGACCCCGGGTTATGGGATGATAATAAGAAGGCCCGGGAAATTACCAGAAAATCCAAGGTGGTTAAAGACAGGAT
TAAATCCCTGAAAAAACTGGATGAGCTCAGGGAAGAAGCAGAGGTTATGATTGAACTGGCCGAAGAGGATGAAACCAACA
GGGAGCTCTGGAGTGAAGTTGAGGATTATATTAACTCCCTGGAAGAAAAGATCGATAAGATGGAATTGAAATTCAGACTG
AATGGGGAATATGATGATTGTAATGCTATATTGTCCATTCACCCCGGAGCCGGTGGGACTGAATCCCAGGACTGGGCAGA
TATGTTATTGCGGATGTATGTTCGCTGGGCAGAAAGCCATGGCTATAAGGTAGAAACCCTTGATTACCTGCCCGGTGATG
AAGCCGGTATTAAGAGGGTAACCCTTCTGATTTCCGGTGAATATAGTTATGGTTATCTAAAAGGTGAAAAGGGTGTTCAC
CGACTGGTAAGGATTTCTCCCTTTGATTCTTCAGGGAGAAGGCATACATCCTTTGCTTCAGTTAATGTTATTCCTGAAAT
TGATGATGATATTGAGGTTGATATAGATACCGGTGATTTGAAAATAGAGACATACCGGGCCAGTGGGGCCGGTGGCCAGC
ATGTCAATAAGACAGATTCAGCTGTAAGGATTACCCATATACCGACCGGTATTGTAGTCCAGTGTCAGAATGAACGTTCC
CAGCATAAAAACAAGAAAATGGCCATGATGATTTTAAAGGCCCGGCTACTGGAATTAAAGAAAGAGGCCCGGGCTGAGAA
AATAGAAGAGCTGAGGGGAGAAAATAAAGAGATTGCCTGGGGTAACCAGATCCGGTCTTATGTTTTCCACCCCTACAATC
TCATAAAGGATCACAGGACCGGTCTGGAGGAAGGTAATGTCCAGAAGGTGATGGATGGTTATATTGATGAGTTTATTGAG
GCCTATCTGAAGACGGGTAATAAAGGCAGGGAATAA

Upstream 100 bases:

>100_bases
ACCCAGAAACTGGAGAACCTGTCCCGGAGGATTTCAGATTTGAGGGATTACCTTTGACTGGGATAATCTCCTTAAATTAA
GAGAAAAACTCAATAAAAAA

Downstream 100 bases:

>100_bases
TGAAGGGAGCTGGATATGAGGAGAAGAGGGTATTTTCTTGAAACATTGATTTTTCTGCTGGTTTTACTGGTGGTAACCCA
GGTATTCTTGCCCGGGCTTG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSDPGLWDDNKKAREITRKSKVVKDRIKSLKKLDELREEAEVMIELAEEDETNRELWSEVEDYINSLEEKIDKMELKFRL
NGEYDDCNAILSIHPGAGGTESQDWADMLLRMYVRWAESHGYKVETLDYLPGDEAGIKRVTLLISGEYSYGYLKGEKGVH
RLVRISPFDSSGRRHTSFASVNVIPEIDDDIEVDIDTGDLKIETYRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNERS
QHKNKKMAMMILKARLLELKKEARAEKIEELRGENKEIAWGNQIRSYVFHPYNLIKDHRTGLEEGNVQKVMDGYIDEFIE
AYLKTGNKGRE

Sequences:

>Translated_331_residues
MSDPGLWDDNKKAREITRKSKVVKDRIKSLKKLDELREEAEVMIELAEEDETNRELWSEVEDYINSLEEKIDKMELKFRL
NGEYDDCNAILSIHPGAGGTESQDWADMLLRMYVRWAESHGYKVETLDYLPGDEAGIKRVTLLISGEYSYGYLKGEKGVH
RLVRISPFDSSGRRHTSFASVNVIPEIDDDIEVDIDTGDLKIETYRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNERS
QHKNKKMAMMILKARLLELKKEARAEKIEELRGENKEIAWGNQIRSYVFHPYNLIKDHRTGLEEGNVQKVMDGYIDEFIE
AYLKTGNKGRE
>Mature_330_residues
SDPGLWDDNKKAREITRKSKVVKDRIKSLKKLDELREEAEVMIELAEEDETNRELWSEVEDYINSLEEKIDKMELKFRLN
GEYDDCNAILSIHPGAGGTESQDWADMLLRMYVRWAESHGYKVETLDYLPGDEAGIKRVTLLISGEYSYGYLKGEKGVHR
LVRISPFDSSGRRHTSFASVNVIPEIDDDIEVDIDTGDLKIETYRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNERSQ
HKNKKMAMMILKARLLELKKEARAEKIEELRGENKEIAWGNQIRSYVFHPYNLIKDHRTGLEEGNVQKVMDGYIDEFIEA
YLKTGNKGRE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=298, Percent_Identity=36.241610738255, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI34577120, Length=255, Percent_Identity=38.0392156862745, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI166795305, Length=199, Percent_Identity=37.1859296482412, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI2367172, Length=326, Percent_Identity=49.6932515337423, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1787462, Length=244, Percent_Identity=43.4426229508197, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=313, Percent_Identity=34.185303514377, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=34.5454545454545, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19921226, Length=272, Percent_Identity=39.7058823529412, Blast_Score=173, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37981; Mature: 37850

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPGLWDDNKKAREITRKSKVVKDRIKSLKKLDELREEAEVMIELAEEDETNRELWSEV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EDYINSLEEKIDKMELKFRLNGEYDDCNAILSIHPGAGGTESQDWADMLLRMYVRWAESH
HHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
GYKVETLDYLPGDEAGIKRVTLLISGEYSYGYLKGEKGVHRLVRISPFDSSGRRHTSFAS
CCEEEEECCCCCCCCCCEEEEEEEECCCCEEEEECCCCHHHEEEECCCCCCCCCCCCEEE
VNVIPEIDDDIEVDIDTGDLKIETYRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNERS
EEECCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCHHH
QHKNKKMAMMILKARLLELKKEARAEKIEELRGENKEIAWGNQIRSYVFHPYNLIKDHRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHCCCHHHHHHHHC
GLEEGNVQKVMDGYIDEFIEAYLKTGNKGRE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDPGLWDDNKKAREITRKSKVVKDRIKSLKKLDELREEAEVMIELAEEDETNRELWSEV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EDYINSLEEKIDKMELKFRLNGEYDDCNAILSIHPGAGGTESQDWADMLLRMYVRWAESH
HHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
GYKVETLDYLPGDEAGIKRVTLLISGEYSYGYLKGEKGVHRLVRISPFDSSGRRHTSFAS
CCEEEEECCCCCCCCCCEEEEEEEECCCCEEEEECCCCHHHEEEECCCCCCCCCCCCEEE
VNVIPEIDDDIEVDIDTGDLKIETYRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNERS
EEECCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCHHH
QHKNKKMAMMILKARLLELKKEARAEKIEELRGENKEIAWGNQIRSYVFHPYNLIKDHRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHCCCHHHHHHHHC
GLEEGNVQKVMDGYIDEFIEAYLKTGNKGRE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]