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The map label for this gene is kojP [H]

Identifier: 220932292

GI number: 220932292

Start: 1547637

End: 1549973

Strand: Reverse

Name: kojP [H]

Synonym: Hore_14560

Alternate gene names: 220932292

Gene position: 1549973-1547637 (Counterclockwise)

Preceding gene: 220932294

Following gene: 220932291

Centisome position: 60.12

GC content: 39.24

Gene sequence:

>2337_bases
ATGAGAAAATACCATCTGAAGAAATTTTACCAGAATCCGCTGTATAAATATGAACCATGGACGATAACTGAGAAAGAATT
TAAGATAGAAAATAATCACCACAATGAAACAATTTTTGCCCTCGGTAATGGTTATATGGGATTAAGGGGGACTCTGGAAG
AAGATTATTCAGGGCCGGAACAGACAACTACCCCTGGTACATACATAAACGGGGTTTATACTGAAGAGAAAATAATATAC
GGAGAAGAGGCACCTGATTTACCGGAGTTTTATCAGACCATGGTCAATCTGGCTGACTGGACTGGAATTAACCTTTACCT
TGGTGATGAAAAATTTGATATGTTACAGGGTAAGGTCAGCGGTTATAAACGGGTTCTTGATATGAAGGAAGGGACTTTAA
CCAGGGAACTTACCTGGGAGAGTCCTCAGGGAAGGAAAGTTAAGTTAACAATAAAAAGGTTTTTATCCCTGGCATCACCA
CATATCGGAGTTATTAAATATAGCTTTACCCCAGTTAATTTTGATGGTACCATAACCGTTGAGTCATCTCTAAAGGGAGA
TGTTCAAAATTATCATACCTTAAGAAATAAAAAACCTTTACAGGTAATAGATAAGGCTTTTTCCGATGGAATGCTGTATC
TTGTTCATAAAATAAAAAACAGTGGTATTGAAGTGGCTACTGCTGTTAAGAATGTAATTTCAGCTGGTTCAGGTGAAATT
GAAACTACTAATAGCCTTATTGAGGGAGATAAGGTTATAAAATTATATAAGATCGAAGCCAGCCAGGGTCAAGAGTATAC
TCTAGAGAAATATGCCTGTCTGTACCATTCCCTGGAGATAGATAAAGAAGATATTGTGGATAAAGTTATTACTGAAGTTA
GTAAGGCCGCTGAAAAGGGCTGGGATTATCTTTTCGAAAACCATTGTCAATTTTTACAGAACTACTGGGATGATGTTGAT
ATTAAAATATACGGAGATGATGCCCTGCAGCAGGCTTTAAGGTTTAATGCCTTTCATCTCATTCAGTCAACCGGGCGTGA
CGGAAAGACTAATATTGCTGCCAAAGGACTCACCGGAGAATATTATGAAGGGCATTATTTCTGGGATACAGAAACATATA
TATTGCCTTTTTATCTCTATAGTAAACCGGAGATTTCAAAGGATCTACTTATGTTCCGTTACTATACCCTTGATGAAGCC
CGGGAAAATGCCCGCAGGATGAGGCTGGATGGGGCCTTATACCCGTGGCGAACGATTAATGGTAAAGAAGCCTCAGGGTT
TTTTATGGGTTCAACCGTACAGTATCATATAGATGCTGACATTGCTTATGCCATTAACCTGTATGTAACTGCAACCAGAG
ATTATGATTTCTTGTTTAATTACGGCGCTGAAATCCTGTTTGAAACTGCCCGGATGTGGGCCAGCCGGGGGAGTTATATT
GAGCTTAAAGGAAATCGCTTCTGTATCAATGAGGTTTGTGGTCCGGATGAATATAAACCGGGAGTCAATAATAATTGTTA
TACCAACTATATGGCTAAATTGAATCTGGAACTGGCATTAGAGGCATACCGGTTGATGGAGAAAAAAGCCCCTGATAATT
TAAAAAAATTAGTTGAAAAGATTAATTTACAGGATTCAGAACTTGAAGCCTGGAAAAGGGCTGCTGACAACATGTATTTA
CCATATAATGAAGAACTGGGAATCCATCCCCAGGACGATTCCTTCCTGTATAAAGACCCTATAGATGTAGATAGCATACC
GGAAGAGGATATTCCTCTGGTTAAAAACTGGCATCCCCTGGTTATATGGCGCTACCAGGTAATAAAACAGGCAGATGTAA
TTTTATTGATGTTATTACTGGGTGACCAATTTACCACCCAGCAGAAGAAAGCCAACTATGATTTTTATGAACCCAAAACT
ACCCATGATTCCTCTTTGTCACCGGCAATTTATAGTATTATTGCCAGTGAAATCGGGTATCATGACTCTGCCTACAATTA
CTTTATGCAAACAGCCAGGCTGGACCTGGATGATTATAATAAAAATGCCTATCAGGGTGTCCATACAGCCTGTATGGCCG
GAACCTGGCTTGCGTTAGTCCAGGGATTTGCTGGAATGCGTAATTATAATGGCGAACTCCACTTTAGCCCCTGTTTACCG
GAAAAGTGGGATGGGTATGAATTTAAGGTTAAGTTCAGGGGAAGACAGCTTAAAATTACTGTAAATGAAGAGGGAGTCAT
GTATAAGCTTATTAAAGGTGAAAAATTGAGCTTCTACCATGGAGATAGGAAACTGGAATTAAATTCAGGGGAGGAGCATA
ATATAGAAATGGGATAA

Upstream 100 bases:

>100_bases
AAATTAATAATAAAATAATTTTTTGATAACACAACTACTTGACTAATATAATTTACTGTTATATAATCAAAATACAACCG
GTTGCAAAGGAGGTAATACG

Downstream 100 bases:

>100_bases
TTTCTAAATGTTTTTCAAATAAAAATTATTAAAAAAATTTAACTGATAAAAGAAGGATTTTTAAAAAATATGTATAATAT
ATTAATTAGGGATAAGCCAT

Product: Kojibiose phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 778; Mature: 778

Protein sequence:

>778_residues
MRKYHLKKFYQNPLYKYEPWTITEKEFKIENNHHNETIFALGNGYMGLRGTLEEDYSGPEQTTTPGTYINGVYTEEKIIY
GEEAPDLPEFYQTMVNLADWTGINLYLGDEKFDMLQGKVSGYKRVLDMKEGTLTRELTWESPQGRKVKLTIKRFLSLASP
HIGVIKYSFTPVNFDGTITVESSLKGDVQNYHTLRNKKPLQVIDKAFSDGMLYLVHKIKNSGIEVATAVKNVISAGSGEI
ETTNSLIEGDKVIKLYKIEASQGQEYTLEKYACLYHSLEIDKEDIVDKVITEVSKAAEKGWDYLFENHCQFLQNYWDDVD
IKIYGDDALQQALRFNAFHLIQSTGRDGKTNIAAKGLTGEYYEGHYFWDTETYILPFYLYSKPEISKDLLMFRYYTLDEA
RENARRMRLDGALYPWRTINGKEASGFFMGSTVQYHIDADIAYAINLYVTATRDYDFLFNYGAEILFETARMWASRGSYI
ELKGNRFCINEVCGPDEYKPGVNNNCYTNYMAKLNLELALEAYRLMEKKAPDNLKKLVEKINLQDSELEAWKRAADNMYL
PYNEELGIHPQDDSFLYKDPIDVDSIPEEDIPLVKNWHPLVIWRYQVIKQADVILLMLLLGDQFTTQQKKANYDFYEPKT
THDSSLSPAIYSIIASEIGYHDSAYNYFMQTARLDLDDYNKNAYQGVHTACMAGTWLALVQGFAGMRNYNGELHFSPCLP
EKWDGYEFKVKFRGRQLKITVNEEGVMYKLIKGEKLSFYHGDRKLELNSGEEHNIEMG

Sequences:

>Translated_778_residues
MRKYHLKKFYQNPLYKYEPWTITEKEFKIENNHHNETIFALGNGYMGLRGTLEEDYSGPEQTTTPGTYINGVYTEEKIIY
GEEAPDLPEFYQTMVNLADWTGINLYLGDEKFDMLQGKVSGYKRVLDMKEGTLTRELTWESPQGRKVKLTIKRFLSLASP
HIGVIKYSFTPVNFDGTITVESSLKGDVQNYHTLRNKKPLQVIDKAFSDGMLYLVHKIKNSGIEVATAVKNVISAGSGEI
ETTNSLIEGDKVIKLYKIEASQGQEYTLEKYACLYHSLEIDKEDIVDKVITEVSKAAEKGWDYLFENHCQFLQNYWDDVD
IKIYGDDALQQALRFNAFHLIQSTGRDGKTNIAAKGLTGEYYEGHYFWDTETYILPFYLYSKPEISKDLLMFRYYTLDEA
RENARRMRLDGALYPWRTINGKEASGFFMGSTVQYHIDADIAYAINLYVTATRDYDFLFNYGAEILFETARMWASRGSYI
ELKGNRFCINEVCGPDEYKPGVNNNCYTNYMAKLNLELALEAYRLMEKKAPDNLKKLVEKINLQDSELEAWKRAADNMYL
PYNEELGIHPQDDSFLYKDPIDVDSIPEEDIPLVKNWHPLVIWRYQVIKQADVILLMLLLGDQFTTQQKKANYDFYEPKT
THDSSLSPAIYSIIASEIGYHDSAYNYFMQTARLDLDDYNKNAYQGVHTACMAGTWLALVQGFAGMRNYNGELHFSPCLP
EKWDGYEFKVKFRGRQLKITVNEEGVMYKLIKGEKLSFYHGDRKLELNSGEEHNIEMG
>Mature_778_residues
MRKYHLKKFYQNPLYKYEPWTITEKEFKIENNHHNETIFALGNGYMGLRGTLEEDYSGPEQTTTPGTYINGVYTEEKIIY
GEEAPDLPEFYQTMVNLADWTGINLYLGDEKFDMLQGKVSGYKRVLDMKEGTLTRELTWESPQGRKVKLTIKRFLSLASP
HIGVIKYSFTPVNFDGTITVESSLKGDVQNYHTLRNKKPLQVIDKAFSDGMLYLVHKIKNSGIEVATAVKNVISAGSGEI
ETTNSLIEGDKVIKLYKIEASQGQEYTLEKYACLYHSLEIDKEDIVDKVITEVSKAAEKGWDYLFENHCQFLQNYWDDVD
IKIYGDDALQQALRFNAFHLIQSTGRDGKTNIAAKGLTGEYYEGHYFWDTETYILPFYLYSKPEISKDLLMFRYYTLDEA
RENARRMRLDGALYPWRTINGKEASGFFMGSTVQYHIDADIAYAINLYVTATRDYDFLFNYGAEILFETARMWASRGSYI
ELKGNRFCINEVCGPDEYKPGVNNNCYTNYMAKLNLELALEAYRLMEKKAPDNLKKLVEKINLQDSELEAWKRAADNMYL
PYNEELGIHPQDDSFLYKDPIDVDSIPEEDIPLVKNWHPLVIWRYQVIKQADVILLMLLLGDQFTTQQKKANYDFYEPKT
THDSSLSPAIYSIIASEIGYHDSAYNYFMQTARLDLDDYNKNAYQGVHTACMAGTWLALVQGFAGMRNYNGELHFSPCLP
EKWDGYEFKVKFRGRQLKITVNEEGVMYKLIKGEKLSFYHGDRKLELNSGEEHNIEMG

Specific function: Hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid. Can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. Inactive when dissaccharides with linkages

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=482, Percent_Identity=22.1991701244813, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI1787575, Length=753, Percent_Identity=29.3492695883134, Blast_Score=349, Evalue=4e-97,
Organism=Saccharomyces cerevisiae, GI6325283, Length=487, Percent_Identity=22.5872689938398, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24583760, Length=362, Percent_Identity=25.1381215469613, Blast_Score=98, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: =2.4.1.230 [H]

Molecular weight: Translated: 89946; Mature: 89946

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYHLKKFYQNPLYKYEPWTITEKEFKIENNHHNETIFALGNGYMGLRGTLEEDYSGPE
CCCCHHHHHHCCCCEEECCEEECCCEEEECCCCCCCEEEEEECCCCCCCCCCHHCCCCCC
QTTTPGTYINGVYTEEKIIYGEEAPDLPEFYQTMVNLADWTGINLYLGDEKFDMLQGKVS
CCCCCCCEEEEEEECCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
GYKRVLDMKEGTLTRELTWESPQGRKVKLTIKRFLSLASPHIGVIKYSFTPVNFDGTITV
HHHHHHHCCCCCEEEEEECCCCCCCEEEEEHHHHHHHCCCCEEEEEEEEEEECCCCEEEE
ESSLKGDVQNYHTLRNKKPLQVIDKAFSDGMLYLVHKIKNSGIEVATAVKNVISAGSGEI
CCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEEHHCCCCCHHHHHHHHHHHCCCCCC
ETTNSLIEGDKVIKLYKIEASQGQEYTLEKYACLYHSLEIDKEDIVDKVITEVSKAAEKG
CCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
WDYLFENHCQFLQNYWDDVDIKIYGDDALQQALRFNAFHLIQSTGRDGKTNIAAKGLTGE
HHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHEEHHHCCCCCCCCEEEECCCCC
YYEGHYFWDTETYILPFYLYSKPEISKDLLMFRYYTLDEARENARRMRLDGALYPWRTIN
CCCCEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECC
GKEASGFFMGSTVQYHIDADIAYAINLYVTATRDYDFLFNYGAEILFETARMWASRGSYI
CCCCCCEEECCEEEEEECCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEE
ELKGNRFCINEVCGPDEYKPGVNNNCYTNYMAKLNLELALEAYRLMEKKAPDNLKKLVEK
EECCCEEEECCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHH
INLQDSELEAWKRAADNMYLPYNEELGIHPQDDSFLYKDPIDVDSIPEEDIPLVKNWHPL
HCCCCHHHHHHHHHHCCEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCE
VIWRYQVIKQADVILLMLLLGDQFTTQQKKANYDFYEPKTTHDSSLSPAIYSIIASEIGY
EEEEEHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
HDSAYNYFMQTARLDLDDYNKNAYQGVHTACMAGTWLALVQGFAGMRNYNGELHFSPCLP
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EKWDGYEFKVKFRGRQLKITVNEEGVMYKLIKGEKLSFYHGDRKLELNSGEEHNIEMG
CCCCCEEEEEEECCEEEEEEECCCCEEEEEECCCEEEEEECCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MRKYHLKKFYQNPLYKYEPWTITEKEFKIENNHHNETIFALGNGYMGLRGTLEEDYSGPE
CCCCHHHHHHCCCCEEECCEEECCCEEEECCCCCCCEEEEEECCCCCCCCCCHHCCCCCC
QTTTPGTYINGVYTEEKIIYGEEAPDLPEFYQTMVNLADWTGINLYLGDEKFDMLQGKVS
CCCCCCCEEEEEEECCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
GYKRVLDMKEGTLTRELTWESPQGRKVKLTIKRFLSLASPHIGVIKYSFTPVNFDGTITV
HHHHHHHCCCCCEEEEEECCCCCCCEEEEEHHHHHHHCCCCEEEEEEEEEEECCCCEEEE
ESSLKGDVQNYHTLRNKKPLQVIDKAFSDGMLYLVHKIKNSGIEVATAVKNVISAGSGEI
CCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEEHHCCCCCHHHHHHHHHHHCCCCCC
ETTNSLIEGDKVIKLYKIEASQGQEYTLEKYACLYHSLEIDKEDIVDKVITEVSKAAEKG
CCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
WDYLFENHCQFLQNYWDDVDIKIYGDDALQQALRFNAFHLIQSTGRDGKTNIAAKGLTGE
HHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHEEHHHCCCCCCCCEEEECCCCC
YYEGHYFWDTETYILPFYLYSKPEISKDLLMFRYYTLDEARENARRMRLDGALYPWRTIN
CCCCEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECC
GKEASGFFMGSTVQYHIDADIAYAINLYVTATRDYDFLFNYGAEILFETARMWASRGSYI
CCCCCCEEECCEEEEEECCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEE
ELKGNRFCINEVCGPDEYKPGVNNNCYTNYMAKLNLELALEAYRLMEKKAPDNLKKLVEK
EECCCEEEECCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHH
INLQDSELEAWKRAADNMYLPYNEELGIHPQDDSFLYKDPIDVDSIPEEDIPLVKNWHPL
HCCCCHHHHHHHHHHCCEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCE
VIWRYQVIKQADVILLMLLLGDQFTTQQKKANYDFYEPKTTHDSSLSPAIYSIIASEIGY
EEEEEHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
HDSAYNYFMQTARLDLDDYNKNAYQGVHTACMAGTWLALVQGFAGMRNYNGELHFSPCLP
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EKWDGYEFKVKFRGRQLKITVNEEGVMYKLIKGEKLSFYHGDRKLELNSGEEHNIEMG
CCCCCEEEEEEECCEEEEEEECCCCEEEEEECCCEEEEEECCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA