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The map label for this gene is ligT [C]

Identifier: 220932027

GI number: 220932027

Start: 1284645

End: 1285214

Strand: Reverse

Name: ligT [C]

Synonym: Hore_11900

Alternate gene names: 220932027

Gene position: 1285214-1284645 (Counterclockwise)

Preceding gene: 220932028

Following gene: 220932026

Centisome position: 49.85

GC content: 34.39

Gene sequence:

>570_bases
ATGAGGTTATTTATTGCTGTTAATATTCCTGACAGGACAAAAGACCTTATAGAACGTAAACTCATCACTATTAAAAGGCA
GGTTAGACAGGGTATAAAATGGGTCAAAAAAGATAACTGGCATATAACCCTGAAATTCATAGGAGAGGTGGGGGATGAGA
AAGTCTCCGAAATAAAGGATTCTTTAGAAAGTATTTCAGAGAGAGTAAAACCGTTTTCTATTACAATTAATGGCCCTGGG
GCTTTTCCAGATATCACAAGGCCAAGGGTAATGTATTTAAGTATTAAAGAAGGTAGTGATAACCTGACTTCTGTCTATAA
ACTGTTAGAAAAAGACTTAATAGATAGAGGATTTAAAGCCGATGATAGGGAATTTACTCCACATCTTACTCTGGCCCGGA
GCAGGCGAAGAACTGATCTTAACCAGTTATCTAAACGTCTTAATAAAGTAGTAGAAAGTCTTGAAGATTTTAAAGCCAGA
GTAAATATAAATAAACTGAGTTTGATGAAAAGTGAGCTTTATAGAACAGGACCAGTGTATAAAGAAATATTTTCAGTTTT
TTTAGAATAA

Upstream 100 bases:

>100_bases
GATGCAGCCATTGCTGAATTAAAGAAAACATTACACTTAAATCCAAATCACCGCGGTGCTCAAAAACTGTTAGAGAAATT
AACAGAGGGTGAGTAATTTT

Downstream 100 bases:

>100_bases
CCCTTGCTTTTTTAAAACATATGTTCTATAATGTTAACTAACAGGACTGAACATATTTTCGGTTTTTGTAATAATTCAAA
AATTGGTGGGAAGGTGTGGT

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MRLFIAVNIPDRTKDLIERKLITIKRQVRQGIKWVKKDNWHITLKFIGEVGDEKVSEIKDSLESISERVKPFSITINGPG
AFPDITRPRVMYLSIKEGSDNLTSVYKLLEKDLIDRGFKADDREFTPHLTLARSRRRTDLNQLSKRLNKVVESLEDFKAR
VNINKLSLMKSELYRTGPVYKEIFSVFLE

Sequences:

>Translated_189_residues
MRLFIAVNIPDRTKDLIERKLITIKRQVRQGIKWVKKDNWHITLKFIGEVGDEKVSEIKDSLESISERVKPFSITINGPG
AFPDITRPRVMYLSIKEGSDNLTSVYKLLEKDLIDRGFKADDREFTPHLTLARSRRRTDLNQLSKRLNKVVESLEDFKAR
VNINKLSLMKSELYRTGPVYKEIFSVFLE
>Mature_189_residues
MRLFIAVNIPDRTKDLIERKLITIKRQVRQGIKWVKKDNWHITLKFIGEVGDEKVSEIKDSLESISERVKPFSITINGPG
AFPDITRPRVMYLSIKEGSDNLTSVYKLLEKDLIDRGFKADDREFTPHLTLARSRRRTDLNQLSKRLNKVVESLEDFKAR
VNINKLSLMKSELYRTGPVYKEIFSVFLE

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 22053; Mature: 22053

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFIAVNIPDRTKDLIERKLITIKRQVRQGIKWVKKDNWHITLKFIGEVGDEKVSEIKD
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHH
SLESISERVKPFSITINGPGAFPDITRPRVMYLSIKEGSDNLTSVYKLLEKDLIDRGFKA
HHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
DDREFTPHLTLARSRRRTDLNQLSKRLNKVVESLEDFKARVNINKLSLMKSELYRTGPVY
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHH
KEIFSVFLE
HHHHHHHCC
>Mature Secondary Structure
MRLFIAVNIPDRTKDLIERKLITIKRQVRQGIKWVKKDNWHITLKFIGEVGDEKVSEIKD
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHH
SLESISERVKPFSITINGPGAFPDITRPRVMYLSIKEGSDNLTSVYKLLEKDLIDRGFKA
HHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
DDREFTPHLTLARSRRRTDLNQLSKRLNKVVESLEDFKARVNINKLSLMKSELYRTGPVY
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHH
KEIFSVFLE
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]