Click here to switch to the map view.

The map label for this gene is sleB [H]

Identifier: 220932013

GI number: 220932013

Start: 1272828

End: 1273421

Strand: Reverse

Name: sleB [H]

Synonym: Hore_11760

Alternate gene names: 220932013

Gene position: 1273421-1272828 (Counterclockwise)

Preceding gene: 220932014

Following gene: 220932011

Centisome position: 49.39

GC content: 38.72

Gene sequence:

>594_bases
TTGAAAAAATATAGTAAATCTATAGCACTATCCTTAATAATAATACTGACAATTTATATTTTAACACCTGCATTATTCAG
TTCTATATTTGTCTATAAAGCCCGGGCTAGTTCAATAGACAGGAAAGATGTCTATCGTGGAGTAGGCATAGCCCTACTTT
TAGTACTGATTGCTAAATTGGGAAAAGCAGCTGAATCTAACACGGAACCGGTGGACAACCCCAGTGACATAAATTTATCT
GGAGAAGACATAGAATTACTGGCCAGGGTTATATATGCTGAAGCCAGAGGAGAACCATATGAAGGGCAGGTAGCTGTTGG
AGCTGTTGTTTTAAACAGGGTTGAGAGCCCTGATTTTCCGGATACTGTTAAGGAAGTAATTTTTCAGGATGGACAGTTTT
CTTCTGTCGATGATGGTCAAATTTATCTAATTCCAAATAAAATTGCCTTTCGTGCTGCCAGGGAGGCATTAAATGGCAGG
GATCCAAGTCAGGGTGCTTTATATTTTTATAATCCCAAAACAGCTAAAACCCTCTGGTGGCTTTCCCAGAGAAAGGTTAC
CGTTAAAATCGGTAACCATGTCTTTGCAAAATAA

Upstream 100 bases:

>100_bases
TGAAATAAAACAACTTAGGAATGAAAATAGTAATTACAGATTATATCTTATTGGAGTTGCTTTGTTAGCCTTACTTGCCA
GATAATGGAGGTTGACCATC

Downstream 100 bases:

>100_bases
GTTATGTTAGTATTAATTACAAAAAGAGGTAGACCAGATGGCCTACCTCTTTTTGTATTTTAAGCCTTTTTATTTTAAGA
CTTTTTGTACTGTAAAAGTA

Product: cell wall hydrolase SleB

Products: NA

Alternate protein names: SCLE; Germination-specific amidase [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MKKYSKSIALSLIIILTIYILTPALFSSIFVYKARASSIDRKDVYRGVGIALLLVLIAKLGKAAESNTEPVDNPSDINLS
GEDIELLARVIYAEARGEPYEGQVAVGAVVLNRVESPDFPDTVKEVIFQDGQFSSVDDGQIYLIPNKIAFRAAREALNGR
DPSQGALYFYNPKTAKTLWWLSQRKVTVKIGNHVFAK

Sequences:

>Translated_197_residues
MKKYSKSIALSLIIILTIYILTPALFSSIFVYKARASSIDRKDVYRGVGIALLLVLIAKLGKAAESNTEPVDNPSDINLS
GEDIELLARVIYAEARGEPYEGQVAVGAVVLNRVESPDFPDTVKEVIFQDGQFSSVDDGQIYLIPNKIAFRAAREALNGR
DPSQGALYFYNPKTAKTLWWLSQRKVTVKIGNHVFAK
>Mature_197_residues
MKKYSKSIALSLIIILTIYILTPALFSSIFVYKARASSIDRKDVYRGVGIALLLVLIAKLGKAAESNTEPVDNPSDINLS
GEDIELLARVIYAEARGEPYEGQVAVGAVVLNRVESPDFPDTVKEVIFQDGQFSSVDDGQIYLIPNKIAFRAAREALNGR
DPSQGALYFYNPKTAKTLWWLSQRKVTVKIGNHVFAK

Specific function: Probable N-acetylmuramyl-L-alanine amidase. Required for spore cortex hydrolysis during germination. May form a complex with some hydrophobic spore component, leading to a stabilization of the enzyme in a spore-bound form [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 21766; Mature: 21766

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYSKSIALSLIIILTIYILTPALFSSIFVYKARASSIDRKDVYRGVGIALLLVLIAKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GKAAESNTEPVDNPSDINLSGEDIELLARVIYAEARGEPYEGQVAVGAVVLNRVESPDFP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCH
DTVKEVIFQDGQFSSVDDGQIYLIPNKIAFRAAREALNGRDPSQGALYFYNPKTAKTLWW
HHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHEE
LSQRKVTVKIGNHVFAK
HHCCEEEEEECCEECCC
>Mature Secondary Structure
MKKYSKSIALSLIIILTIYILTPALFSSIFVYKARASSIDRKDVYRGVGIALLLVLIAKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GKAAESNTEPVDNPSDINLSGEDIELLARVIYAEARGEPYEGQVAVGAVVLNRVESPDFP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCH
DTVKEVIFQDGQFSSVDDGQIYLIPNKIAFRAAREALNGRDPSQGALYFYNPKTAKTLWW
HHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHEE
LSQRKVTVKIGNHVFAK
HHCCEEEEEECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752358; 8081503; 10197998 [H]