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The map label for this gene is gyrB [H]

Identifier: 220932011

GI number: 220932011

Start: 1268149

End: 1270062

Strand: Reverse

Name: gyrB [H]

Synonym: Hore_11740

Alternate gene names: 220932011

Gene position: 1270062-1268149 (Counterclockwise)

Preceding gene: 220932013

Following gene: 220932010

Centisome position: 49.26

GC content: 39.03

Gene sequence:

>1914_bases
ATGTCGCTATTTGAAGGGGCTGTATATGGTGCGGAACAGATTGAGATATTAAAAGGTCTGGAAGCAGTCAGGAAAAGACC
CGGAATGTATATAGGAAGCACAGGAATCCGGGGCCTTCACCATCTGGTCTGGGAAGTAGTTGATAACAGTATTGATGAGT
ACCTGGCTGGATATGGTGATAAGATTGAAGTTACAATTAACGAAGATAATAGTATAACCGTTGAAGACTGGGGGCGGGGT
ATACCGGCTGAAGAACACCCCGAGACTGGATTACCGGCTGCCCAGGTAGTAATGACTACCCTTCATGCCGGGGGAAAGTT
TAATAATAACAGTTATAAAGTATCCGGTGGTCTTCACGGTGTCGGTATATCTGTAGTTAATGCCCTCTCTGAGTGGCTGG
AACTGACTACATACTGGAATGGAAATGAGTATTACCAGCGCTATGAGAGGGGATATCCCAAAACAAAATTCCAGAAACTG
GGGACCAGTAATAGAAATGGTACTATTATCTCTTTTAAACCAGATCCAGAGATTTTTGATGTAACTGAGTTTAAATATGA
AACCCTGGCCCATCGTCTTCAGGAATCTGCTTTTTTAAATAAAGAATTATCCCTCATTTTGATAGACAAAAGATCAGATA
AAACTAAAAAAGAAGTTTTTCAATATGACGGGGGTATAAAAGCCTTTGTAGAATACTTAAATAAAAACCGTCAGGTTATA
AATGAGGATATTATATATACTGAAAAAGAGAATGGGGATTACTACATTGAAGTAGCCCTTCAGTACAATGAGGGGTACAA
TGAAAGGATCTTTTCATATGCCAATAATATCAATACTGTTGATGGTGGTTACCACTTAACTGGTTTCAAGACAGCATTAA
CAAAAGCAATAAATAACTTTGCCAAACAAAATAACCTGCTTAAACAGAGTGACCCTACTTTAACCGGTAATGATGTCCGG
GAGGGTTTAACTGCAGTTGTTAATGTCAAATTACCAAATCCCCAGTTTGAGGGCCAGACCAAATCTAAACTGGGTAATAG
TGAAATAAGGAGTATTGTTGAGAGCAATATTTATGATTACCTCAGCCTCTATTTAGATTCCAATCCGGAAGTTGGTAAAA
AGATAGTAGACAAGGCTATACAGGCGATAAGAGCCCGTCAGGCTTCCAAAAAAGCCAGGGAACTAACCCGGAGAAAAAAT
GCTCTAAATTCTCATTCCTTACCGGGTAAACTGGCCGATTGTGCCAGCCGTAAACCAGAAAAGAGTGAACTATTTCTGGT
TGAGGGAGATTCAGCAGGCGGTTCAGCCAAACAGGGACGCAACAGGCATTTCCAGGCGATTTTACCGTTAAAAGGGAAAA
TAATGAACGTGGAACGGGCTCGTCTTGATAAAATATTGAGTAATAATGAAATAGCTACCATTATTTCGGCCCTGGGTACC
GGTATTGGTCAGGAATTTAATATTGATAAACTACGGTATCATAAAATTATTATAATGACTGATGCCGATGTTGATGGTGC
CCATATTGCAACTCTTTTATTAACTTTATTCTACCGGTATATGCCTGAACTAATCAAAGAAGGTTATATTTATATAGCCC
AACCACCTTTATATAAAGTCTCATGGAAGAAAAATATTCGCTATCTTTATACTGATAAAGAATTAGAATCTCTAAAAAGG
GAGCTCGAAAATAAATCATATTCCATTCAGCGGTATAAGGGGCTCGGTGAAATGAATCCAAGACAGCTATGGGAGACTAC
CATGAACCCTGAAACACGTAAACTACTACGGATTGAAATTGATGATGAAATTGAAGCAGATGAAATTTTTACCCGCTTAA
TGGGTTCCAATGCCAGTCTAAGGCGTGAGTTTATTATTTCCAATGCCGATATGGTTGATGAATTGGATATCTAA

Upstream 100 bases:

>100_bases
ATACTTGATATTATAACAAATAATTAACTATTTCAAGAAACAGCTGGTAAAAATCTGTTCGTTTTGATATAATATAGTGC
GAAAAGGAAGGTGAAAGGAC

Downstream 100 bases:

>100_bases
GATAGTTTTTATATCTATAAAATTACTGAATATTATTACCGGGGCAGGAGATAATAAGATTAAAAAGAAAAGGGGGCTTG
CATGACTGAAAAAGACATTA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MSLFEGAVYGAEQIEILKGLEAVRKRPGMYIGSTGIRGLHHLVWEVVDNSIDEYLAGYGDKIEVTINEDNSITVEDWGRG
IPAEEHPETGLPAAQVVMTTLHAGGKFNNNSYKVSGGLHGVGISVVNALSEWLELTTYWNGNEYYQRYERGYPKTKFQKL
GTSNRNGTIISFKPDPEIFDVTEFKYETLAHRLQESAFLNKELSLILIDKRSDKTKKEVFQYDGGIKAFVEYLNKNRQVI
NEDIIYTEKENGDYYIEVALQYNEGYNERIFSYANNINTVDGGYHLTGFKTALTKAINNFAKQNNLLKQSDPTLTGNDVR
EGLTAVVNVKLPNPQFEGQTKSKLGNSEIRSIVESNIYDYLSLYLDSNPEVGKKIVDKAIQAIRARQASKKARELTRRKN
ALNSHSLPGKLADCASRKPEKSELFLVEGDSAGGSAKQGRNRHFQAILPLKGKIMNVERARLDKILSNNEIATIISALGT
GIGQEFNIDKLRYHKIIIMTDADVDGAHIATLLLTLFYRYMPELIKEGYIYIAQPPLYKVSWKKNIRYLYTDKELESLKR
ELENKSYSIQRYKGLGEMNPRQLWETTMNPETRKLLRIEIDDEIEADEIFTRLMGSNASLRREFIISNADMVDELDI

Sequences:

>Translated_637_residues
MSLFEGAVYGAEQIEILKGLEAVRKRPGMYIGSTGIRGLHHLVWEVVDNSIDEYLAGYGDKIEVTINEDNSITVEDWGRG
IPAEEHPETGLPAAQVVMTTLHAGGKFNNNSYKVSGGLHGVGISVVNALSEWLELTTYWNGNEYYQRYERGYPKTKFQKL
GTSNRNGTIISFKPDPEIFDVTEFKYETLAHRLQESAFLNKELSLILIDKRSDKTKKEVFQYDGGIKAFVEYLNKNRQVI
NEDIIYTEKENGDYYIEVALQYNEGYNERIFSYANNINTVDGGYHLTGFKTALTKAINNFAKQNNLLKQSDPTLTGNDVR
EGLTAVVNVKLPNPQFEGQTKSKLGNSEIRSIVESNIYDYLSLYLDSNPEVGKKIVDKAIQAIRARQASKKARELTRRKN
ALNSHSLPGKLADCASRKPEKSELFLVEGDSAGGSAKQGRNRHFQAILPLKGKIMNVERARLDKILSNNEIATIISALGT
GIGQEFNIDKLRYHKIIIMTDADVDGAHIATLLLTLFYRYMPELIKEGYIYIAQPPLYKVSWKKNIRYLYTDKELESLKR
ELENKSYSIQRYKGLGEMNPRQLWETTMNPETRKLLRIEIDDEIEADEIFTRLMGSNASLRREFIISNADMVDELDI
>Mature_636_residues
SLFEGAVYGAEQIEILKGLEAVRKRPGMYIGSTGIRGLHHLVWEVVDNSIDEYLAGYGDKIEVTINEDNSITVEDWGRGI
PAEEHPETGLPAAQVVMTTLHAGGKFNNNSYKVSGGLHGVGISVVNALSEWLELTTYWNGNEYYQRYERGYPKTKFQKLG
TSNRNGTIISFKPDPEIFDVTEFKYETLAHRLQESAFLNKELSLILIDKRSDKTKKEVFQYDGGIKAFVEYLNKNRQVIN
EDIIYTEKENGDYYIEVALQYNEGYNERIFSYANNINTVDGGYHLTGFKTALTKAINNFAKQNNLLKQSDPTLTGNDVRE
GLTAVVNVKLPNPQFEGQTKSKLGNSEIRSIVESNIYDYLSLYLDSNPEVGKKIVDKAIQAIRARQASKKARELTRRKNA
LNSHSLPGKLADCASRKPEKSELFLVEGDSAGGSAKQGRNRHFQAILPLKGKIMNVERARLDKILSNNEIATIISALGTG
IGQEFNIDKLRYHKIIIMTDADVDGAHIATLLLTLFYRYMPELIKEGYIYIAQPPLYKVSWKKNIRYLYTDKELESLKRE
LENKSYSIQRYKGLGEMNPRQLWETTMNPETRKLLRIEIDDEIEADEIFTRLMGSNASLRREFIISNADMVDELDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=614, Percent_Identity=25.0814332247557, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI19913408, Length=631, Percent_Identity=24.2472266244057, Blast_Score=136, Evalue=7e-32,
Organism=Escherichia coli, GI48994957, Length=551, Percent_Identity=57.1687840290381, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1789408, Length=633, Percent_Identity=38.8625592417062, Blast_Score=435, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17535065, Length=649, Percent_Identity=24.3451463790447, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=546, Percent_Identity=24.1758241758242, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=180, Percent_Identity=28.8888888888889, Blast_Score=83, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=619, Percent_Identity=25.3634894991922, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI17136538, Length=668, Percent_Identity=23.502994011976, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72317; Mature: 72186

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFEGAVYGAEQIEILKGLEAVRKRPGMYIGSTGIRGLHHLVWEVVDNSIDEYLAGYGD
CCCCCCHHCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
KIEVTINEDNSITVEDWGRGIPAEEHPETGLPAAQVVMTTLHAGGKFNNNSYKVSGGLHG
EEEEEECCCCEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCH
VGISVVNALSEWLELTTYWNGNEYYQRYERGYPKTKFQKLGTSNRNGTIISFKPDPEIFD
HHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCCCEEE
VTEFKYETLAHRLQESAFLNKELSLILIDKRSDKTKKEVFQYDGGIKAFVEYLNKNRQVI
CHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCEE
NEDIIYTEKENGDYYIEVALQYNEGYNERIFSYANNINTVDGGYHLTGFKTALTKAINNF
CCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHH
AKQNNLLKQSDPTLTGNDVREGLTAVVNVKLPNPQFEGQTKSKLGNSEIRSIVESNIYDY
HHHCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHH
LSLYLDSNPEVGKKIVDKAIQAIRARQASKKARELTRRKNALNSHSLPGKLADCASRKPE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCC
KSELFLVEGDSAGGSAKQGRNRHFQAILPLKGKIMNVERARLDKILSNNEIATIISALGT
CCCEEEEECCCCCCCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHCCCHHHHHHHHHHC
GIGQEFNIDKLRYHKIIIMTDADVDGAHIATLLLTLFYRYMPELIKEGYIYIAQPPLYKV
CCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEE
SWKKNIRYLYTDKELESLKRELENKSYSIQRYKGLGEMNPRQLWETTMNPETRKLLRIEI
ECCCCCEEEEECHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEE
DDEIEADEIFTRLMGSNASLRREFIISNADMVDELDI
CCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
SLFEGAVYGAEQIEILKGLEAVRKRPGMYIGSTGIRGLHHLVWEVVDNSIDEYLAGYGD
CCCCCHHCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
KIEVTINEDNSITVEDWGRGIPAEEHPETGLPAAQVVMTTLHAGGKFNNNSYKVSGGLHG
EEEEEECCCCEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCH
VGISVVNALSEWLELTTYWNGNEYYQRYERGYPKTKFQKLGTSNRNGTIISFKPDPEIFD
HHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCCCEEE
VTEFKYETLAHRLQESAFLNKELSLILIDKRSDKTKKEVFQYDGGIKAFVEYLNKNRQVI
CHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCEE
NEDIIYTEKENGDYYIEVALQYNEGYNERIFSYANNINTVDGGYHLTGFKTALTKAINNF
CCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHH
AKQNNLLKQSDPTLTGNDVREGLTAVVNVKLPNPQFEGQTKSKLGNSEIRSIVESNIYDY
HHHCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHH
LSLYLDSNPEVGKKIVDKAIQAIRARQASKKARELTRRKNALNSHSLPGKLADCASRKPE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCC
KSELFLVEGDSAGGSAKQGRNRHFQAILPLKGKIMNVERARLDKILSNNEIATIISALGT
CCCEEEEECCCCCCCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHCCCHHHHHHHHHHC
GIGQEFNIDKLRYHKIIIMTDADVDGAHIATLLLTLFYRYMPELIKEGYIYIAQPPLYKV
CCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEE
SWKKNIRYLYTDKELESLKRELENKSYSIQRYKGLGEMNPRQLWETTMNPETRKLLRIEI
ECCCCCEEEEECHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEE
DDEIEADEIFTRLMGSNASLRREFIISNADMVDELDI
CCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]