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The map label for this gene is dapF

Identifier: 220931996

GI number: 220931996

Start: 1249517

End: 1250371

Strand: Reverse

Name: dapF

Synonym: Hore_11570

Alternate gene names: 220931996

Gene position: 1250371-1249517 (Counterclockwise)

Preceding gene: 220931997

Following gene: 220931995

Centisome position: 48.5

GC content: 37.66

Gene sequence:

>855_bases
ATGGAACTAAGTTTTACAAAAATGCATGGAACTGGAAATGATTTTATTATGGTAAATGGTAGTAACTATCCTGATTTAGA
TTTTAGTAAGCTTGCCAGACAATTATGCCGCAGACATTTTTCAATAGGTGCAGATGGTTTAATTATTGTGTTACCACCGG
AGAGTGTTGAACATGACTTTAGAATGAGGATCTTCAATGCTGACGGTAGTGAGGCTGAAATGTGTGGAAATGGCATTCGA
TGTTTTGCCCACTATCTCCGGGAAAATAATTTGACTACCAGGGATGTACTTAAAATTGAAACCCTGGCCGGTATTATAAC
TCCTGAGATAGTATCTTATAATGGTGATAAGAGTCTAATAAAAGTAAATATGGGAAGGCCTCATTTTAAATCAGAAGAAA
TTCCTGTTAATATAGAAGATGAGCTTGATTATGTTAAAAATTTCCCATTAAAAATAGGAAATAAGAAGTTAAACATTAAT
TGTGTTTCGATGGGTAACCCCCATACTATTATATTTGTAGAAGATGTAAACCAGATACCGGTTTCAACCTGGGGTCAGGA
AATAGAACACAATCCGCTGTTTCCTCAAAAAACCAATGTAGAGTTTATTCAGATTCAATCCGAAGATGAAATTATTATGA
GGGTCTGGGAAAGGGGTAGTGGAATAACCCTGGCCTGTGGTACTGGAGCCTGTGCCTCAGTAGTAGCTGGTATTAAAAAT
GGTCTCCTCAAAAACATGGTGACTGTACACTTGCCCGGGGGAGACCTTAATATTGAATGGCAGGAACAGGATGTATTTAT
GACAGGTCCTGCAGAGAGTGTTTACACAGGAAAAATCGTAATACAGGAGGGATAA

Upstream 100 bases:

>100_bases
GAAGGAAAGGACCAGATGGTGTATAAACATGCAATTTCAACTATCATACCACAGGAAAAGGTGGATATCCAGTTAAGTTC
AGAGTAAAGGTGGGTATTAA

Downstream 100 bases:

>100_bases
GCAATGGAAAATGCTGATAGAATTAAAAACCTGCCGCCATATTTATTCGCAGAAATTGATAAAATGATTGCCAGAGCAAA
AAAAGAGGGAGTAGATGTAA

Product: Diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MELSFTKMHGTGNDFIMVNGSNYPDLDFSKLARQLCRRHFSIGADGLIIVLPPESVEHDFRMRIFNADGSEAEMCGNGIR
CFAHYLRENNLTTRDVLKIETLAGIITPEIVSYNGDKSLIKVNMGRPHFKSEEIPVNIEDELDYVKNFPLKIGNKKLNIN
CVSMGNPHTIIFVEDVNQIPVSTWGQEIEHNPLFPQKTNVEFIQIQSEDEIIMRVWERGSGITLACGTGACASVVAGIKN
GLLKNMVTVHLPGGDLNIEWQEQDVFMTGPAESVYTGKIVIQEG

Sequences:

>Translated_284_residues
MELSFTKMHGTGNDFIMVNGSNYPDLDFSKLARQLCRRHFSIGADGLIIVLPPESVEHDFRMRIFNADGSEAEMCGNGIR
CFAHYLRENNLTTRDVLKIETLAGIITPEIVSYNGDKSLIKVNMGRPHFKSEEIPVNIEDELDYVKNFPLKIGNKKLNIN
CVSMGNPHTIIFVEDVNQIPVSTWGQEIEHNPLFPQKTNVEFIQIQSEDEIIMRVWERGSGITLACGTGACASVVAGIKN
GLLKNMVTVHLPGGDLNIEWQEQDVFMTGPAESVYTGKIVIQEG
>Mature_284_residues
MELSFTKMHGTGNDFIMVNGSNYPDLDFSKLARQLCRRHFSIGADGLIIVLPPESVEHDFRMRIFNADGSEAEMCGNGIR
CFAHYLRENNLTTRDVLKIETLAGIITPEIVSYNGDKSLIKVNMGRPHFKSEEIPVNIEDELDYVKNFPLKIGNKKLNIN
CVSMGNPHTIIFVEDVNQIPVSTWGQEIEHNPLFPQKTNVEFIQIQSEDEIIMRVWERGSGITLACGTGACASVVAGIKN
GLLKNMVTVHLPGGDLNIEWQEQDVFMTGPAESVYTGKIVIQEG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=279, Percent_Identity=41.2186379928315, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_HALOH (B8CX90)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508904.1
- ProteinModelPortal:   B8CX90
- SMR:   B8CX90
- GeneID:   7312902
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_11570
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31683; Mature: 31683

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSFTKMHGTGNDFIMVNGSNYPDLDFSKLARQLCRRHFSIGADGLIIVLPPESVEHDF
CCCEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCE
RMRIFNADGSEAEMCGNGIRCFAHYLRENNLTTRDVLKIETLAGIITPEIVSYNGDKSLI
EEEEECCCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEE
KVNMGRPHFKSEEIPVNIEDELDYVKNFPLKIGNKKLNINCVSMGNPHTIIFVEDVNQIP
EEECCCCCCCCCCCCEECHHHHHHHHCCCEEECCEEEEEEEEECCCCEEEEEEECCCCCC
VSTWGQEIEHNPLFPQKTNVEFIQIQSEDEIIMRVWERGSGITLACGTGACASVVAGIKN
CHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
GLLKNMVTVHLPGGDLNIEWQEQDVFMTGPAESVYTGKIVIQEG
HHHHCEEEEEECCCCEEEEEECCEEEEECCCCCEEEEEEEEECC
>Mature Secondary Structure
MELSFTKMHGTGNDFIMVNGSNYPDLDFSKLARQLCRRHFSIGADGLIIVLPPESVEHDF
CCCEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCE
RMRIFNADGSEAEMCGNGIRCFAHYLRENNLTTRDVLKIETLAGIITPEIVSYNGDKSLI
EEEEECCCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEE
KVNMGRPHFKSEEIPVNIEDELDYVKNFPLKIGNKKLNINCVSMGNPHTIIFVEDVNQIP
EEECCCCCCCCCCCCEECHHHHHHHHCCCEEECCEEEEEEEEECCCCEEEEEEECCCCCC
VSTWGQEIEHNPLFPQKTNVEFIQIQSEDEIIMRVWERGSGITLACGTGACASVVAGIKN
CHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
GLLKNMVTVHLPGGDLNIEWQEQDVFMTGPAESVYTGKIVIQEG
HHHHCEEEEEECCCCEEEEEECCEEEEECCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA