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The map label for this gene is def

Identifier: 220931832

GI number: 220931832

Start: 1080303

End: 1080767

Strand: Direct

Name: def

Synonym: Hore_09890

Alternate gene names: 220931832

Gene position: 1080303-1080767 (Clockwise)

Preceding gene: 220931831

Following gene: 220931833

Centisome position: 41.9

GC content: 41.29

Gene sequence:

>465_bases
GTGCCAGTTTTACAGATTAGAAAAATAGGTGATCCTGTTTTAAGAAGTAAAGCTAAGCCTGTTACAGAAATAACAAAAAA
GACTCTTAGTTTAATAGATAATATGGTTGAAACCATGTACCAGGCCGAAGGGGTTGGTCTGGCTGCCCCCCAGGTTGGGG
TTTCAAAGAGAATTATTGTTGTGGATACCGGGGAAGGTCAGGGCCTGATTGAACTTATTAACCCTGAAATTATAGAAACC
GAGGGCAAAGATATTATGGAAGAAGGTTGTCTGAGTGTACCAGGACAGACTGGAAAAGTTATACGTGCCAGCAAAGTTAC
GGTTAAGGGTTTGAACCGGGGCGGGAAAGAAGTCAGGATCAGGGCTGAAGGTTTTCTTGCCAGGGCCTTTCAGCATGAAA
TTGATCATCTAAACGGAATTTTATTTATTGATAAAGTGGTCAGGATTGGAGAAGAAATGATTTAA

Upstream 100 bases:

>100_bases
ATTACAGAAATCTTTCTTACCTGACCAGGGTAGGAATGTCAACATTATTATTGATGTTGATCCTGTTAAAATGATATAAT
TTGTTAGGGAGGTGGCGTTA

Downstream 100 bases:

>100_bases
GAAGGTGGTATTGGCATGAATATAGTGTTTATGGGAAGTCCTGATTTTGCAGTACCCGGTCTTGAGAAATTGTATAATGA
GCCCGGTATAACAATAAAGG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MPVLQIRKIGDPVLRSKAKPVTEITKKTLSLIDNMVETMYQAEGVGLAAPQVGVSKRIIVVDTGEGQGLIELINPEIIET
EGKDIMEEGCLSVPGQTGKVIRASKVTVKGLNRGGKEVRIRAEGFLARAFQHEIDHLNGILFIDKVVRIGEEMI

Sequences:

>Translated_154_residues
MPVLQIRKIGDPVLRSKAKPVTEITKKTLSLIDNMVETMYQAEGVGLAAPQVGVSKRIIVVDTGEGQGLIELINPEIIET
EGKDIMEEGCLSVPGQTGKVIRASKVTVKGLNRGGKEVRIRAEGFLARAFQHEIDHLNGILFIDKVVRIGEEMI
>Mature_153_residues
PVLQIRKIGDPVLRSKAKPVTEITKKTLSLIDNMVETMYQAEGVGLAAPQVGVSKRIIVVDTGEGQGLIELINPEIIETE
GKDIMEEGCLSVPGQTGKVIRASKVTVKGLNRGGKEVRIRAEGFLARAFQHEIDHLNGILFIDKVVRIGEEMI

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=166, Percent_Identity=32.5301204819277, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI1789682, Length=155, Percent_Identity=46.4516129032258, Blast_Score=138, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24645728, Length=168, Percent_Identity=35.7142857142857, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24645726, Length=168, Percent_Identity=32.7380952380952, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_HALOH (B8CWS6)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508740.1
- ProteinModelPortal:   B8CWS6
- SMR:   B8CWS6
- GeneID:   7314577
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_09890
- HOGENOM:   HBG665227
- OMA:   MILPINI
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 16799; Mature: 16668

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLQIRKIGDPVLRSKAKPVTEITKKTLSLIDNMVETMYQAEGVGLAAPQVGVSKRIIV
CCCCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
VDTGEGQGLIELINPEIIETEGKDIMEEGCLSVPGQTGKVIRASKVTVKGLNRGGKEVRI
EECCCCCCEEEECCCEEEECCCHHHHHHCCCCCCCCCCCEEEECEEEEECCCCCCCEEEE
RAEGFLARAFQHEIDHLNGILFIDKVVRIGEEMI
EHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVLQIRKIGDPVLRSKAKPVTEITKKTLSLIDNMVETMYQAEGVGLAAPQVGVSKRIIV
CCCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
VDTGEGQGLIELINPEIIETEGKDIMEEGCLSVPGQTGKVIRASKVTVKGLNRGGKEVRI
EECCCCCCEEEECCCEEEECCCHHHHHHCCCCCCCCCCCEEEECEEEEECCCCCCCEEEE
RAEGFLARAFQHEIDHLNGILFIDKVVRIGEEMI
EHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA