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The map label for this gene is priA [H]

Identifier: 220931831

GI number: 220931831

Start: 1078044

End: 1080281

Strand: Direct

Name: priA [H]

Synonym: Hore_09880

Alternate gene names: 220931831

Gene position: 1078044-1080281 (Clockwise)

Preceding gene: 220931830

Following gene: 220931832

Centisome position: 41.81

GC content: 37.35

Gene sequence:

>2238_bases
ATGAAGAAATATGCCAGGGTTGTTGTTGATATAAAGGCTCCCGGTCTTAACCGTTCGTATGATTATAAGATACCGGAAGA
TCTGGAAAATAAAATAAGTATTGGTACTGCCGTAAAAGTACCTTTTGGAAAGCAGATTATAACCGGTTTTATAACCGGTT
TTTCTTCTCAAACGAGGGTTGAATCAGTAAAGATTAAAGAAATTATGAATATAATATCTGAAGAACCCCTTTTTGATAAA
AGTATGTTAAAATTATTTGCATGGGTAGCTTCTTATTATAAAACATATTTGATAAATGTAATTAAAATAGCAATTCCCAC
CGGTCTTGTTTCTGGAAAGGTCGGCAAAATTAAAAAAAGATATGTTAAATTGTTACTTGCTGAAGAGGAAGCCAGGCGGG
TTATTGAAAAAATAAATACCAGGGCTCCCAAGCAGGCCCTGGTTGTGAAGGTATTAATGGAAAACGATAATATTGATTAT
ACTGTAAGTAAACTGGCTGAAACAGCCGGAACCTCTCCCGGTACTGTCTACAGGCTCATAGAAAAGGGCTATTTAGGGTA
TGTTAAAAAAGTTAAAATAAGAAGACCTGATTTGGGAGAAAAAGAAGATGTTAAGCCGGTTAAACTTACTCCGGTTCAAA
AGCAGGTCATTGAGAAAATTGAAGACAGTATAGAAAAGGGAGTCCCAGATACTTTTTTACTACACGGGGTTACTGGAAGC
GGTAAGACCGAGGTTTACATTAATACTGCATCCAGGGCAGTAGAACAGGGTAAAGGTGTTATCATTCTGGTTCCAGAAAT
ATCCCTTACTCCTTTAATGGTCAGGCGTTTTTATAACAAATTTGGGGAACAGGTAGCTGTAATGCACAGTAACCTCTCAC
TGGGAGAAAGGTATGATGAATGGCGTCGTCTAAAAGAAGGAAAGGCTAAAATAGTTATCGGGGCCAGATCAGCTGTATTT
TCCCCGGTCAGGAATCTGGGATTAATTGTTATTGATGAAGAACATGAAAATTCTTATAAACAGGGAGAAAATCCATATTA
CCATGCCCGTGAAGTAGCTGTTATGAGGGGTAAACTGGAAAATGCTTCAGTGGTATTGGGGTCTGCAACTCCATCACTTG
AAAGTTATTACCGGGCCCGGAAGGGATTTTATAAATATTTGTCGCTACCAGAAAGGATAAATCAGGAAAGACTCCCACCG
GTTGAAATTATAGACATGAGAGATGAATTGAGACAGGGTAATACCAGTATTTTTAGTAGATCACTGCGGGATGCAATTAA
CAAAGCCTTATCAAATAAGGAGCAGGTGATTATTTTTTTAAATAGAAGGGGTTATGCTAATTTTGTACTTTGCCGGGAAT
GTGGCCATGTTATAAAGTGCAATAATTGTGATATTTCTCTTACCTACCACGCTGACGAAGACCTGTTAAGATGTCATTAT
TGTGATTATACCAGGGGTAAACCTGATAAATGTCCCCTGTGTGGTAGTAAATTAATTAAAGAGTTTGGAATAGGTACGGA
AAAACTCCAGAATGAACTGGCTAATGAATTTGTCGGGGCGCGAGTTGATAGACTTGATGTGGATACAACAACGAGAAAGG
GTTCCCATCACAGGATATTAAAGAGATTTGAGGATGGGAAAACTGATATTCTGGTAGGGACACAGATGGTGGCAAAAGGG
CATGATTATCCTAATGTGTCAGTGGTTGGTGTAATTTCTGCAGATACAATCCTCAATTTACCTGATTTTCGTTCTTCTGA
AAGAACCTTTCAACTTTTAACCCAGGTAGCCGGAAGGACAGGGCGGGGTTCTAAACAGGGACGGGTTATTATTCAGACCT
ATAACCCTGACCATTATAGTATTAAATCGTCTAGTCATCACAATTTTAGAGAATTTTATCGTCATGAGATTAGGTTAAGA
CATGCATTAAATTATCCTCCCTTTTCTCTGTTAGTAAATATAATCATAAGTGGGTTAAAGGAGAAAATTGTAGTCAAGAA
AGCTCAAAAATTATATAATTTTTTAATGCAGTATAACCAGCAAATAAATGAAATGCTCGGGCCTGCTCCGGCCCCTGTTT
ATAGATTACGAAAAAAATACAGGTGGCAGATAATTCTTAAATTTAAAAGCTATAAAAAGAGGAATCACGTTTTGTCTGAA
TTACAGAAATCTTTCTTACCTGACCAGGGTAGGAATGTCAACATTATTATTGATGTTGATCCTGTTAAAATGATATAA

Upstream 100 bases:

>100_bases
TGGTTACCTGTATCTGGAGATTTATCACTTTTTATGTCGTTTTAACAGTTGGAGGTATTTTTGCCCTCCGGGAAATAACC
AGAAGTAGGGGTCAGGAATC

Downstream 100 bases:

>100_bases
TTTGTTAGGGAGGTGGCGTTAGTGCCAGTTTTACAGATTAGAAAAATAGGTGATCCTGTTTTAAGAAGTAAAGCTAAGCC
TGTTACAGAAATAACAAAAA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MKKYARVVVDIKAPGLNRSYDYKIPEDLENKISIGTAVKVPFGKQIITGFITGFSSQTRVESVKIKEIMNIISEEPLFDK
SMLKLFAWVASYYKTYLINVIKIAIPTGLVSGKVGKIKKRYVKLLLAEEEARRVIEKINTRAPKQALVVKVLMENDNIDY
TVSKLAETAGTSPGTVYRLIEKGYLGYVKKVKIRRPDLGEKEDVKPVKLTPVQKQVIEKIEDSIEKGVPDTFLLHGVTGS
GKTEVYINTASRAVEQGKGVIILVPEISLTPLMVRRFYNKFGEQVAVMHSNLSLGERYDEWRRLKEGKAKIVIGARSAVF
SPVRNLGLIVIDEEHENSYKQGENPYYHAREVAVMRGKLENASVVLGSATPSLESYYRARKGFYKYLSLPERINQERLPP
VEIIDMRDELRQGNTSIFSRSLRDAINKALSNKEQVIIFLNRRGYANFVLCRECGHVIKCNNCDISLTYHADEDLLRCHY
CDYTRGKPDKCPLCGSKLIKEFGIGTEKLQNELANEFVGARVDRLDVDTTTRKGSHHRILKRFEDGKTDILVGTQMVAKG
HDYPNVSVVGVISADTILNLPDFRSSERTFQLLTQVAGRTGRGSKQGRVIIQTYNPDHYSIKSSSHHNFREFYRHEIRLR
HALNYPPFSLLVNIIISGLKEKIVVKKAQKLYNFLMQYNQQINEMLGPAPAPVYRLRKKYRWQIILKFKSYKKRNHVLSE
LQKSFLPDQGRNVNIIIDVDPVKMI

Sequences:

>Translated_745_residues
MKKYARVVVDIKAPGLNRSYDYKIPEDLENKISIGTAVKVPFGKQIITGFITGFSSQTRVESVKIKEIMNIISEEPLFDK
SMLKLFAWVASYYKTYLINVIKIAIPTGLVSGKVGKIKKRYVKLLLAEEEARRVIEKINTRAPKQALVVKVLMENDNIDY
TVSKLAETAGTSPGTVYRLIEKGYLGYVKKVKIRRPDLGEKEDVKPVKLTPVQKQVIEKIEDSIEKGVPDTFLLHGVTGS
GKTEVYINTASRAVEQGKGVIILVPEISLTPLMVRRFYNKFGEQVAVMHSNLSLGERYDEWRRLKEGKAKIVIGARSAVF
SPVRNLGLIVIDEEHENSYKQGENPYYHAREVAVMRGKLENASVVLGSATPSLESYYRARKGFYKYLSLPERINQERLPP
VEIIDMRDELRQGNTSIFSRSLRDAINKALSNKEQVIIFLNRRGYANFVLCRECGHVIKCNNCDISLTYHADEDLLRCHY
CDYTRGKPDKCPLCGSKLIKEFGIGTEKLQNELANEFVGARVDRLDVDTTTRKGSHHRILKRFEDGKTDILVGTQMVAKG
HDYPNVSVVGVISADTILNLPDFRSSERTFQLLTQVAGRTGRGSKQGRVIIQTYNPDHYSIKSSSHHNFREFYRHEIRLR
HALNYPPFSLLVNIIISGLKEKIVVKKAQKLYNFLMQYNQQINEMLGPAPAPVYRLRKKYRWQIILKFKSYKKRNHVLSE
LQKSFLPDQGRNVNIIIDVDPVKMI
>Mature_745_residues
MKKYARVVVDIKAPGLNRSYDYKIPEDLENKISIGTAVKVPFGKQIITGFITGFSSQTRVESVKIKEIMNIISEEPLFDK
SMLKLFAWVASYYKTYLINVIKIAIPTGLVSGKVGKIKKRYVKLLLAEEEARRVIEKINTRAPKQALVVKVLMENDNIDY
TVSKLAETAGTSPGTVYRLIEKGYLGYVKKVKIRRPDLGEKEDVKPVKLTPVQKQVIEKIEDSIEKGVPDTFLLHGVTGS
GKTEVYINTASRAVEQGKGVIILVPEISLTPLMVRRFYNKFGEQVAVMHSNLSLGERYDEWRRLKEGKAKIVIGARSAVF
SPVRNLGLIVIDEEHENSYKQGENPYYHAREVAVMRGKLENASVVLGSATPSLESYYRARKGFYKYLSLPERINQERLPP
VEIIDMRDELRQGNTSIFSRSLRDAINKALSNKEQVIIFLNRRGYANFVLCRECGHVIKCNNCDISLTYHADEDLLRCHY
CDYTRGKPDKCPLCGSKLIKEFGIGTEKLQNELANEFVGARVDRLDVDTTTRKGSHHRILKRFEDGKTDILVGTQMVAKG
HDYPNVSVVGVISADTILNLPDFRSSERTFQLLTQVAGRTGRGSKQGRVIIQTYNPDHYSIKSSSHHNFREFYRHEIRLR
HALNYPPFSLLVNIIISGLKEKIVVKKAQKLYNFLMQYNQQINEMLGPAPAPVYRLRKKYRWQIILKFKSYKKRNHVLSE
LQKSFLPDQGRNVNIIIDVDPVKMI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=741, Percent_Identity=34.5479082321188, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1787357, Length=178, Percent_Identity=28.6516853932584, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 84967; Mature: 84967

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYARVVVDIKAPGLNRSYDYKIPEDLENKISIGTAVKVPFGKQIITGFITGFSSQTRV
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHEECCEEEECCCCHHHHHHHHHCCCCCHHH
ESVKIKEIMNIISEEPLFDKSMLKLFAWVASYYKTYLINVIKIAIPTGLVSGKVGKIKKR
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
YVKLLLAEEEARRVIEKINTRAPKQALVVKVLMENDNIDYTVSKLAETAGTSPGTVYRLI
HHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHH
EKGYLGYVKKVKIRRPDLGEKEDVKPVKLTPVQKQVIEKIEDSIEKGVPDTFLLHGVTGS
HHCHHHHHHEEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
GKTEVYINTASRAVEQGKGVIILVPEISLTPLMVRRFYNKFGEQVAVMHSNLSLGERYDE
CCEEEEEECHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEHCCCCCHHHHHHH
WRRLKEGKAKIVIGARSAVFSPVRNLGLIVIDEEHENSYKQGENPYYHAREVAVMRGKLE
HHHHHCCCEEEEEECHHHHHHHHHHCCEEEEECCCCCHHHCCCCCCHHHHHHHHHHCCCC
NASVVLGSATPSLESYYRARKGFYKYLSLPERINQERLPPVEIIDMRDELRQGNTSIFSR
CCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEECCHHHHCCCCHHHHHH
SLRDAINKALSNKEQVIIFLNRRGYANFVLCRECGHVIKCNNCDISLTYHADEDLLRCHY
HHHHHHHHHHCCCCEEEEEEECCCCCCEEEEHHCCCEEEECCCEEEEEEECCCCHHEEEE
CDYTRGKPDKCPLCGSKLIKEFGIGTEKLQNELANEFVGARVDRLDVDTTTRKGSHHRIL
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHH
KRFEDGKTDILVGTQMVAKGHDYPNVSVVGVISADTILNLPDFRSSERTFQLLTQVAGRT
HHHCCCCCEEEECHHHHHCCCCCCCEEEEEEEECCCEECCCCCCCCHHHHHHHHHHHCCC
GRGSKQGRVIIQTYNPDHYSIKSSSHHNFREFYRHEIRLRHALNYPPFSLLVNIIISGLK
CCCCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EKIVVKKAQKLYNFLMQYNQQINEMLGPAPAPVYRLRKKYRWQIILKFKSYKKRNHVLSE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEEEEHHHHHHHHHHHH
LQKSFLPDQGRNVNIIIDVDPVKMI
HHHHHCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MKKYARVVVDIKAPGLNRSYDYKIPEDLENKISIGTAVKVPFGKQIITGFITGFSSQTRV
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHEECCEEEECCCCHHHHHHHHHCCCCCHHH
ESVKIKEIMNIISEEPLFDKSMLKLFAWVASYYKTYLINVIKIAIPTGLVSGKVGKIKKR
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
YVKLLLAEEEARRVIEKINTRAPKQALVVKVLMENDNIDYTVSKLAETAGTSPGTVYRLI
HHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHH
EKGYLGYVKKVKIRRPDLGEKEDVKPVKLTPVQKQVIEKIEDSIEKGVPDTFLLHGVTGS
HHCHHHHHHEEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
GKTEVYINTASRAVEQGKGVIILVPEISLTPLMVRRFYNKFGEQVAVMHSNLSLGERYDE
CCEEEEEECHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEHCCCCCHHHHHHH
WRRLKEGKAKIVIGARSAVFSPVRNLGLIVIDEEHENSYKQGENPYYHAREVAVMRGKLE
HHHHHCCCEEEEEECHHHHHHHHHHCCEEEEECCCCCHHHCCCCCCHHHHHHHHHHCCCC
NASVVLGSATPSLESYYRARKGFYKYLSLPERINQERLPPVEIIDMRDELRQGNTSIFSR
CCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEECCHHHHCCCCHHHHHH
SLRDAINKALSNKEQVIIFLNRRGYANFVLCRECGHVIKCNNCDISLTYHADEDLLRCHY
HHHHHHHHHHCCCCEEEEEEECCCCCCEEEEHHCCCEEEECCCEEEEEEECCCCHHEEEE
CDYTRGKPDKCPLCGSKLIKEFGIGTEKLQNELANEFVGARVDRLDVDTTTRKGSHHRIL
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHH
KRFEDGKTDILVGTQMVAKGHDYPNVSVVGVISADTILNLPDFRSSERTFQLLTQVAGRT
HHHCCCCCEEEECHHHHHCCCCCCCEEEEEEEECCCEECCCCCCCCHHHHHHHHHHHCCC
GRGSKQGRVIIQTYNPDHYSIKSSSHHNFREFYRHEIRLRHALNYPPFSLLVNIIISGLK
CCCCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EKIVVKKAQKLYNFLMQYNQQINEMLGPAPAPVYRLRKKYRWQIILKFKSYKKRNHVLSE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEEEEHHHHHHHHHHHH
LQKSFLPDQGRNVNIIIDVDPVKMI
HHHHHCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]