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The map label for this gene is ddl

Identifier: 220931741

GI number: 220931741

Start: 969420

End: 970451

Strand: Direct

Name: ddl

Synonym: Hore_08980

Alternate gene names: 220931741

Gene position: 969420-970451 (Clockwise)

Preceding gene: 220931740

Following gene: 220931742

Centisome position: 37.6

GC content: 38.95

Gene sequence:

>1032_bases
GTGAGTAAATTAAGGCTTGGAGTTTTGTTTGGGGGACGCTCTCAGGAACATGAAGTTTCTGTTATGTCGGCCAGGTCAGT
TGTCAAAATGGCCAAAAAAGAAAAATATAAGGTAATTCCCTTTGGTATAACCAAAAAAGGTCAATGGGTCGGACCCGAGG
AATCGTGGAATATACTGAACAATGGAATAAATGAAGTTAAAGCCAGTAAAGATAGGTGTATTACAGAGAGTGTCAGGGTA
TTCCTGGATCACAAACTGGATATTGTTTTTCCTGTTTTACACGGTCCTTATGGAGAGGATGGCAAGCTTCAGGGATTTTT
AGATTGTCTTGATATCCCCTATATAGGAGCCAGTGTTTTATCTTCGGCAGCCGGTATGGATAAGGAAATAATGAAAAACT
TATTTAGCTATCATAAAATTCCCCAGGCCAGATACAGGGTTTATCGACAGAATCATTTAGAAAATGGACTGGATGGCATT
ATCTCAGAAATAGATCGATTTTTAGGATGGCCCTGTTTTGTTAAACCGGCTAATATGGGATCAAGCATCGGGGTCAGCAA
GGTTCATTCTCCTGGGGAAGTAAAAAAGGCCCTGGAAAAGGGATTTTATTATGACCGTAAACTTATTTTTGAAGAATTTG
TAGAGGGGCGAGAAATAGAGTGTTCAGTGCTGGGTAATGACCAGGTTGAAGCTTCACTACCCGGTGAAATTCTTCCCGGA
AAAGAGTTTTATGATTACGAAGCTAAATATAAAGATAACAAAACCCGACTGGTGATACCTGCCTCTCTGGATGAAAGTGT
TATTGATAAGGCCAGAAACCTGGCAATTAAAGCTTTTAAAGCTATTGATGGTAATGGGTTTGCCAGAGTTGATTTTTTCT
TAAAGAATAACGGAGTGCTTTTAGTAAATGAGATTAATACAATACCTGGTTTTACTCAATATAGTATGTACCCCAAATTA
TGGGAAGCAACCGGTCTTAACTACCCTGATTTAATAGATAAATTAATAGAACTTGCCCTGGAGGACAGATAG

Upstream 100 bases:

>100_bases
TAAATTATTTTAAGTAATATTATCGTTAAAATGCTGTAATTATTAATAAGGAAAATAAAGTTATGTATATAAGAAAGGAG
ATTAAGAAAGGGGTCTTGGT

Downstream 100 bases:

>100_bases
GTTTAGGCCAAAACAAACCAGGGGGCGACAAACATGGAATTGGATATTATGCATGTAGATATGGATGCCTTTTTTGCCTC
TGTGGAACAGCTCGACAATC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSKLRLGVLFGGRSQEHEVSVMSARSVVKMAKKEKYKVIPFGITKKGQWVGPEESWNILNNGINEVKASKDRCITESVRV
FLDHKLDIVFPVLHGPYGEDGKLQGFLDCLDIPYIGASVLSSAAGMDKEIMKNLFSYHKIPQARYRVYRQNHLENGLDGI
ISEIDRFLGWPCFVKPANMGSSIGVSKVHSPGEVKKALEKGFYYDRKLIFEEFVEGREIECSVLGNDQVEASLPGEILPG
KEFYDYEAKYKDNKTRLVIPASLDESVIDKARNLAIKAFKAIDGNGFARVDFFLKNNGVLLVNEINTIPGFTQYSMYPKL
WEATGLNYPDLIDKLIELALEDR

Sequences:

>Translated_343_residues
MSKLRLGVLFGGRSQEHEVSVMSARSVVKMAKKEKYKVIPFGITKKGQWVGPEESWNILNNGINEVKASKDRCITESVRV
FLDHKLDIVFPVLHGPYGEDGKLQGFLDCLDIPYIGASVLSSAAGMDKEIMKNLFSYHKIPQARYRVYRQNHLENGLDGI
ISEIDRFLGWPCFVKPANMGSSIGVSKVHSPGEVKKALEKGFYYDRKLIFEEFVEGREIECSVLGNDQVEASLPGEILPG
KEFYDYEAKYKDNKTRLVIPASLDESVIDKARNLAIKAFKAIDGNGFARVDFFLKNNGVLLVNEINTIPGFTQYSMYPKL
WEATGLNYPDLIDKLIELALEDR
>Mature_342_residues
SKLRLGVLFGGRSQEHEVSVMSARSVVKMAKKEKYKVIPFGITKKGQWVGPEESWNILNNGINEVKASKDRCITESVRVF
LDHKLDIVFPVLHGPYGEDGKLQGFLDCLDIPYIGASVLSSAAGMDKEIMKNLFSYHKIPQARYRVYRQNHLENGLDGII
SEIDRFLGWPCFVKPANMGSSIGVSKVHSPGEVKKALEKGFYYDRKLIFEEFVEGREIECSVLGNDQVEASLPGEILPGK
EFYDYEAKYKDNKTRLVIPASLDESVIDKARNLAIKAFKAIDGNGFARVDFFLKNNGVLLVNEINTIPGFTQYSMYPKLW
EATGLNYPDLIDKLIELALEDR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=355, Percent_Identity=41.6901408450704, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1786280, Length=337, Percent_Identity=32.3442136498516, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_HALOH (B8CWI5)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508649.1
- ProteinModelPortal:   B8CWI5
- SMR:   B8CWI5
- GeneID:   7314894
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_08980
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38656; Mature: 38525

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLRLGVLFGGRSQEHEVSVMSARSVVKMAKKEKYKVIPFGITKKGQWVGPEESWNILN
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHH
NGINEVKASKDRCITESVRVFLDHKLDIVFPVLHGPYGEDGKLQGFLDCLDIPYIGASVL
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHH
SSAAGMDKEIMKNLFSYHKIPQARYRVYRQNHLENGLDGIISEIDRFLGWPCFVKPANMG
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCC
SSIGVSKVHSPGEVKKALEKGFYYDRKLIFEEFVEGREIECSVLGNDQVEASLPGEILPG
CCCCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCC
KEFYDYEAKYKDNKTRLVIPASLDESVIDKARNLAIKAFKAIDGNGFARVDFFLKNNGVL
CCCCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHEECCCCEEEEEEEEECCCEE
LVNEINTIPGFTQYSMYPKLWEATGLNYPDLIDKLIELALEDR
EEEECCCCCCCHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKLRLGVLFGGRSQEHEVSVMSARSVVKMAKKEKYKVIPFGITKKGQWVGPEESWNILN
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHH
NGINEVKASKDRCITESVRVFLDHKLDIVFPVLHGPYGEDGKLQGFLDCLDIPYIGASVL
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHH
SSAAGMDKEIMKNLFSYHKIPQARYRVYRQNHLENGLDGIISEIDRFLGWPCFVKPANMG
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCC
SSIGVSKVHSPGEVKKALEKGFYYDRKLIFEEFVEGREIECSVLGNDQVEASLPGEILPG
CCCCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCC
KEFYDYEAKYKDNKTRLVIPASLDESVIDKARNLAIKAFKAIDGNGFARVDFFLKNNGVL
CCCCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHEECCCCEEEEEEEEECCCEE
LVNEINTIPGFTQYSMYPKLWEATGLNYPDLIDKLIELALEDR
EEEECCCCCCCHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA