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The map label for this gene is trpS2 [H]

Identifier: 220931740

GI number: 220931740

Start: 968335

End: 969336

Strand: Direct

Name: trpS2 [H]

Synonym: Hore_08970

Alternate gene names: 220931740

Gene position: 968335-969336 (Clockwise)

Preceding gene: 220931738

Following gene: 220931741

Centisome position: 37.56

GC content: 39.32

Gene sequence:

>1002_bases
ATGGCAGAGAAAAAAAAGAAAAGGATATTAACAGGAGATAGGCCGACCGGGAAATTACACCTGGGTCATTATGTAGGGAG
TCTGCAAAACAGGGTAAGATTACAAAAAGAATATGATACCTTTTTAATAATTGCTGATGTTCAGGCCCTGACAACCAATT
TTGAAACACCTGAAAAGCTGTCCCGGGATGTCAGGCAGGTAGCCACAGATTATCTTGCTGTTGGGATAGACCCTGAAGTG
ACAACAATATTTGTTCAATCTATGGTACCTGAAATTGCAGAGTTGACTGTTTTTTATTCTATGATTGTTACTGTTAACCA
ATTACGGCATAATCCAACTATTAAGTCTGAAGCTGCCCAGTATGGCTATAATGATATGACATATGGTTTTCTGGGATATC
CGGTTAGTCAGGCAGCTGACATTACTTTCTGTAAAGCCAATCTGGTCCCCGTAGGTGAAGATCAGCTTCCTCATATTGAG
CAGACCAGAAGAATTGTAAGAAAATTTAACAATCTATATGCTCCTGTATTTCCAGAACCCGAAGCACTTGTCAGTGATAT
ACCAAGGTTGGTTGGGCTTGATGGCAAAAGCAAAATGAGTAAAAGCCTGGATAATGCTATTTATCTTTCTGATAGTGAAG
AAGAGGTTAATAGAAAGGTTCAAAAGGCGGTAACTGACCCGGCCCGTATCAGGAAAACTGATCCGGGACATCCTGAAGTT
TGTACTGTTTTTGAGTATCATAAGGCTTTTAATTCAGATGAAGTTAATGAAATTGAGGAAAAATGTCGGGCTGGAAAAAT
TGGCTGTGTGGCCTGTAAAAAGCGTCTGGCAGAGGTTTTAAATAATCTACTGGAACCAATGAGGGAGCGCCGTGTTAAAT
ATGAACAGAATCCTGATATTGTTGACGAAATATTAATGGCAGGCACTAAAAAAGTCAGGGAAATAGGCAAAAAGACACTT
GAAGAAGTACGGGAGGCAATGCATTTAAATTATTTTAAGTAA

Upstream 100 bases:

>100_bases
ATTTTCCTTTTGTGGAACCTTATGCCCTTTGCTTTTTATATGGGATGGGCATTTTTTTATAATGCATATTATTGAGGAAA
TAACAGGAGGGGTGGTTATA

Downstream 100 bases:

>100_bases
TATTATCGTTAAAATGCTGTAATTATTAATAAGGAAAATAAAGTTATGTATATAAGAAAGGAGATTAAGAAAGGGGTCTT
GGTGTGAGTAAATTAAGGCT

Product: Tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase II; TrpRS II; Tryptophanyl-tRNA synthetase II [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAEKKKKRILTGDRPTGKLHLGHYVGSLQNRVRLQKEYDTFLIIADVQALTTNFETPEKLSRDVRQVATDYLAVGIDPEV
TTIFVQSMVPEIAELTVFYSMIVTVNQLRHNPTIKSEAAQYGYNDMTYGFLGYPVSQAADITFCKANLVPVGEDQLPHIE
QTRRIVRKFNNLYAPVFPEPEALVSDIPRLVGLDGKSKMSKSLDNAIYLSDSEEEVNRKVQKAVTDPARIRKTDPGHPEV
CTVFEYHKAFNSDEVNEIEEKCRAGKIGCVACKKRLAEVLNNLLEPMRERRVKYEQNPDIVDEILMAGTKKVREIGKKTL
EEVREAMHLNYFK

Sequences:

>Translated_333_residues
MAEKKKKRILTGDRPTGKLHLGHYVGSLQNRVRLQKEYDTFLIIADVQALTTNFETPEKLSRDVRQVATDYLAVGIDPEV
TTIFVQSMVPEIAELTVFYSMIVTVNQLRHNPTIKSEAAQYGYNDMTYGFLGYPVSQAADITFCKANLVPVGEDQLPHIE
QTRRIVRKFNNLYAPVFPEPEALVSDIPRLVGLDGKSKMSKSLDNAIYLSDSEEEVNRKVQKAVTDPARIRKTDPGHPEV
CTVFEYHKAFNSDEVNEIEEKCRAGKIGCVACKKRLAEVLNNLLEPMRERRVKYEQNPDIVDEILMAGTKKVREIGKKTL
EEVREAMHLNYFK
>Mature_332_residues
AEKKKKRILTGDRPTGKLHLGHYVGSLQNRVRLQKEYDTFLIIADVQALTTNFETPEKLSRDVRQVATDYLAVGIDPEVT
TIFVQSMVPEIAELTVFYSMIVTVNQLRHNPTIKSEAAQYGYNDMTYGFLGYPVSQAADITFCKANLVPVGEDQLPHIEQ
TRRIVRKFNNLYAPVFPEPEALVSDIPRLVGLDGKSKMSKSLDNAIYLSDSEEEVNRKVQKAVTDPARIRKTDPGHPEVC
TVFEYHKAFNSDEVNEIEEKCRAGKIGCVACKKRLAEVLNNLLEPMRERRVKYEQNPDIVDEILMAGTKKVREIGKKTLE
EVREAMHLNYFK

Specific function: Catalyzes the formation of 5'adenyl-Trp and tRNA(Trp) but with 5-fold less activity than trpRS. Increases the solubility of the nitric oxide synthase oxygenase (nos), as well as its affinity for substrate L-arginine and its nitric-oxide synthase activity.

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=326, Percent_Identity=33.4355828220859, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI41352700, Length=186, Percent_Identity=39.247311827957, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1789786, Length=327, Percent_Identity=32.1100917431193, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71982800, Length=331, Percent_Identity=31.1178247734139, Blast_Score=140, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71982793, Length=331, Percent_Identity=31.1178247734139, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320474, Length=337, Percent_Identity=28.7833827893175, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24666151, Length=332, Percent_Identity=31.6265060240964, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 37893; Mature: 37762

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKKKKRILTGDRPTGKLHLGHYVGSLQNRVRLQKEYDTFLIIADVQALTTNFETPEKL
CCCCHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCHHHH
SRDVRQVATDYLAVGIDPEVTTIFVQSMVPEIAELTVFYSMIVTVNQLRHNPTIKSEAAQ
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
YGYNDMTYGFLGYPVSQAADITFCKANLVPVGEDQLPHIEQTRRIVRKFNNLYAPVFPEP
CCCCCCCCCHHCCCHHHCCCEEEEECCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCH
EALVSDIPRLVGLDGKSKMSKSLDNAIYLSDSEEEVNRKVQKAVTDPARIRKTDPGHPEV
HHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCHHHHCCCCCCCCCH
CTVFEYHKAFNSDEVNEIEEKCRAGKIGCVACKKRLAEVLNNLLEPMRERRVKYEQNPDI
HHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
VDEILMAGTKKVREIGKKTLEEVREAMHLNYFK
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AEKKKKRILTGDRPTGKLHLGHYVGSLQNRVRLQKEYDTFLIIADVQALTTNFETPEKL
CCCHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCHHHH
SRDVRQVATDYLAVGIDPEVTTIFVQSMVPEIAELTVFYSMIVTVNQLRHNPTIKSEAAQ
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
YGYNDMTYGFLGYPVSQAADITFCKANLVPVGEDQLPHIEQTRRIVRKFNNLYAPVFPEP
CCCCCCCCCHHCCCHHHCCCEEEEECCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCH
EALVSDIPRLVGLDGKSKMSKSLDNAIYLSDSEEEVNRKVQKAVTDPARIRKTDPGHPEV
HHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCHHHHCCCCCCCCCH
CTVFEYHKAFNSDEVNEIEEKCRAGKIGCVACKKRLAEVLNNLLEPMRERRVKYEQNPDI
HHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
VDEILMAGTKKVREIGKKTLEEVREAMHLNYFK
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]