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The map label for this gene is ogt [H]

Identifier: 220931660

GI number: 220931660

Start: 877915

End: 878430

Strand: Direct

Name: ogt [H]

Synonym: Hore_08160

Alternate gene names: 220931660

Gene position: 877915-878430 (Clockwise)

Preceding gene: 220931659

Following gene: 220931661

Centisome position: 34.05

GC content: 40.31

Gene sequence:

>516_bases
GTGATAAAAAATTATTATCCTTCTCCACTGGGTTTAATGGAAATTACATTATCTTTAGATGGTCTGGTAAGCATAACCTT
TGTTAAAAATGTAGACAATAAATATAAAAACCTTTATAGTATTAACTTTACCTGTAAACAGCTTTATGAACTAAATAAAG
AGGTTTATAATCAGTTAAATGAATACTTTACCGGCCAGAGGCGTAATTTTAACCTGCCTCTGGCCCCGGCAGGGACAGAT
TTTCAGAAAAAAGTGTGGCAGGAGCTGTTAAATATCCCCTATGGGGAGACATGTTCCTACGGAGATATTGCCAGGGCATT
AGGAAAACCAGGGGCAGCCCGGGCTGTAGGGAGGGCCAATGGAAAAAATCCTCTACCTATAGTAATACCGTGTCATCGGG
TTATAGGCAGCAATGGAGAATTAAAGGGTTATGCCTGTGGCCTCTGGCGAAAAAGGTGGCTTATAGAACACGAGTCTTAT
TATTCTAAACAGGGACAGTACCGGGGCTTATTATGA

Upstream 100 bases:

>100_bases
ATAGATTATTGACCAGGCGGGAAGCCTATATGTTGCTGGGGTATTTTTTAAGAAAATTTGATATACCCGAATCTTTAAAA
AGATTCAGGGGTGAAATAAA

Downstream 100 bases:

>100_bases
CCTATTAACTCTTATTTTTTAAATTACATTTGAATAAGGATGGTTTTAAATTTGTTTATAAATCATAAAGGAGTGAGGAG
TATGGCTAAAGAATTACCCG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MIKNYYPSPLGLMEITLSLDGLVSITFVKNVDNKYKNLYSINFTCKQLYELNKEVYNQLNEYFTGQRRNFNLPLAPAGTD
FQKKVWQELLNIPYGETCSYGDIARALGKPGAARAVGRANGKNPLPIVIPCHRVIGSNGELKGYACGLWRKRWLIEHESY
YSKQGQYRGLL

Sequences:

>Translated_171_residues
MIKNYYPSPLGLMEITLSLDGLVSITFVKNVDNKYKNLYSINFTCKQLYELNKEVYNQLNEYFTGQRRNFNLPLAPAGTD
FQKKVWQELLNIPYGETCSYGDIARALGKPGAARAVGRANGKNPLPIVIPCHRVIGSNGELKGYACGLWRKRWLIEHESY
YSKQGQYRGLL
>Mature_171_residues
MIKNYYPSPLGLMEITLSLDGLVSITFVKNVDNKYKNLYSINFTCKQLYELNKEVYNQLNEYFTGQRRNFNLPLAPAGTD
FQKKVWQELLNIPYGETCSYGDIARALGKPGAARAVGRANGKNPLPIVIPCHRVIGSNGELKGYACGLWRKRWLIEHESY
YSKQGQYRGLL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=170, Percent_Identity=32.9411764705882, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=55.2380952380952, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1788542, Length=107, Percent_Identity=47.6635514018692, Blast_Score=97, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=50, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=50, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=54.320987654321, Blast_Score=100, Evalue=9e-23,
Organism=Drosophila melanogaster, GI17137554, Length=113, Percent_Identity=39.8230088495575, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19490; Mature: 19490

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNYYPSPLGLMEITLSLDGLVSITFVKNVDNKYKNLYSINFTCKQLYELNKEVYNQLN
CCCCCCCCCCCCEEEEEECCCEEEEEEEHHCCHHHHHEEEECHHHHHHHHHHHHHHHHHH
EYFTGQRRNFNLPLAPAGTDFQKKVWQELLNIPYGETCSYGDIARALGKPGAARAVGRAN
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHCCC
GKNPLPIVIPCHRVIGSNGELKGYACGLWRKRWLIEHESYYSKQGQYRGLL
CCCCCEEEEECHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIKNYYPSPLGLMEITLSLDGLVSITFVKNVDNKYKNLYSINFTCKQLYELNKEVYNQLN
CCCCCCCCCCCCEEEEEECCCEEEEEEEHHCCHHHHHEEEECHHHHHHHHHHHHHHHHHH
EYFTGQRRNFNLPLAPAGTDFQKKVWQELLNIPYGETCSYGDIARALGKPGAARAVGRAN
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHCCC
GKNPLPIVIPCHRVIGSNGELKGYACGLWRKRWLIEHESYYSKQGQYRGLL
CCCCCEEEEECHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]