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The map label for this gene is glpK

Identifier: 220931653

GI number: 220931653

Start: 868990

End: 870489

Strand: Direct

Name: glpK

Synonym: Hore_08090

Alternate gene names: 220931653

Gene position: 868990-870489 (Clockwise)

Preceding gene: 220931652

Following gene: 220931654

Centisome position: 33.71

GC content: 42.33

Gene sequence:

>1500_bases
GTGAATAAAAAGTATATAATGGCCATTGATCAGGGGACAACCAGTACCAGAGCTATGATATTTAATCACCGGGGTGAGGT
TGTCAGTAAGTCACAGCAGGAATTTAGTCAGATATACCCTAAACCGGGCTGGGTTGAACACAGTCCTGATGAGATCTGGG
TTTCGATCCTTGGTGTCATGAGTGGCGCCTTAAGGTCAAAAGGTATTTCACCCAAAGAAATTGCTGCCATTGGAATTACA
AATCAGCGAGAGACTACAGTAATCTGGGATAAAAATACAGATGAACCTGTCTATAATGCTATTGTCTGGCAGTGTCGGAG
AACTACAGATATCTGTCAGGAATTAAAAAATAATGGGCTGGAAGATAAATTTAAAGAAAAAACGGGTCTTGTCCTTGACC
CTTACTTTTCAGGGACCAAAATTAAGTGGATTTTAGACAATGTTGCCGGAGCCAGGCAGAAAGCTAAAAATGGTGACCTT
CTGTTTGGCACAATTGATAGCTGGTTGATATGGAAGCTTACCGGGGGAAAAGTCCATGTCACTGATTATACTAATGCATC
CCGGACTTTATTATATAACATTCATGAGTTAGACTGGGATGAAGAATTATTAGATATCCTTGGTATTCCAGAGAGTATAC
TTCCTGAAGTTAAAGCGAGTTCGACAATTTACGGTAATACAGTACCCTATCATTTTTTCGGAGAGGAGGTTCCTATTTCT
GGTATTGCCGGGGACCAGCAGGCTGCTACCTTTGCCCAGGGTTGTTATGAAAAGGGGATGACTAAAATTACCTATGGTAC
CGGTGGATTTATGTTAATGAATACCGGAAAAGAAGCGGTTACTTCAGATAATGGACTGTTAACAACAATTGCCTGGGGCC
TGGATGATGGTATATATTATGCCCTTGAGGGAAGTATTTTTAACGCCGGTTCCGCGATTCAGTGGCTAAGGGATGAACTT
GACTTAATTGAAGATGCTGCTGATTCTGAGTATTTTGCCAGTAAGGTAGAAGATACCGGAGGTGTCTATGTTGTTCCTGC
TTTTACAGGACTCGGTGCCCCTTACTGGGATCCCAGGGCCCGGGGGATAATTGTAGGTCTAACCAGGGGAACTAATAAAA
ATCATCTTATCCGGGCCACTATTGAGTCACTGATTTATCAGAGTAAAGATGTTTTAATGGCAATGGTTGAAGATTCGGGT
CTTGATTTAAAGGATATAAAGGTTGATGGAGGTGCTGCTGCTAATAATCTTTTGTTACAGTTCCTATCAGATATGACCGG
ATCCCGGGTTGAAAGACCCATTAACACTGAAACGACGGCAGCCGGGGCAGCTTATCTGGCTGGTCTGGCTATTGATTTCT
GGACAGACCAGGAGGAAGTACTTAAGATAAGGAAAGTTGACCGGGAGTTTAATCCTGATATGTCAGGGGAAAAAAGAGAG
CAATTATACAGGGGATGGAAAAAAGCTATTAATGCAGCCCTTAGCTGGAGTAAGACTTAA

Upstream 100 bases:

>100_bases
AATAACCAGGTCGGAGAGAGGAGAAAGGCCTGTTTAATGTCTATGTGCACATTAAACAGGCTATTGTTATTTTATGACAT
ATTTTAATGGAGGGATTTAT

Downstream 100 bases:

>100_bases
TTATAAATTGATTTTTAACTGATAATATTATATAATTAATTTAGGTAATAAAATTAACTACTAAAATAGAATAAAAATGG
TGGAGAACAAGAGAGAACCA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNKKYIMAIDQGTTSTRAMIFNHRGEVVSKSQQEFSQIYPKPGWVEHSPDEIWVSILGVMSGALRSKGISPKEIAAIGIT
NQRETTVIWDKNTDEPVYNAIVWQCRRTTDICQELKNNGLEDKFKEKTGLVLDPYFSGTKIKWILDNVAGARQKAKNGDL
LFGTIDSWLIWKLTGGKVHVTDYTNASRTLLYNIHELDWDEELLDILGIPESILPEVKASSTIYGNTVPYHFFGEEVPIS
GIAGDQQAATFAQGCYEKGMTKITYGTGGFMLMNTGKEAVTSDNGLLTTIAWGLDDGIYYALEGSIFNAGSAIQWLRDEL
DLIEDAADSEYFASKVEDTGGVYVVPAFTGLGAPYWDPRARGIIVGLTRGTNKNHLIRATIESLIYQSKDVLMAMVEDSG
LDLKDIKVDGGAAANNLLLQFLSDMTGSRVERPINTETTAAGAAYLAGLAIDFWTDQEEVLKIRKVDREFNPDMSGEKRE
QLYRGWKKAINAALSWSKT

Sequences:

>Translated_499_residues
MNKKYIMAIDQGTTSTRAMIFNHRGEVVSKSQQEFSQIYPKPGWVEHSPDEIWVSILGVMSGALRSKGISPKEIAAIGIT
NQRETTVIWDKNTDEPVYNAIVWQCRRTTDICQELKNNGLEDKFKEKTGLVLDPYFSGTKIKWILDNVAGARQKAKNGDL
LFGTIDSWLIWKLTGGKVHVTDYTNASRTLLYNIHELDWDEELLDILGIPESILPEVKASSTIYGNTVPYHFFGEEVPIS
GIAGDQQAATFAQGCYEKGMTKITYGTGGFMLMNTGKEAVTSDNGLLTTIAWGLDDGIYYALEGSIFNAGSAIQWLRDEL
DLIEDAADSEYFASKVEDTGGVYVVPAFTGLGAPYWDPRARGIIVGLTRGTNKNHLIRATIESLIYQSKDVLMAMVEDSG
LDLKDIKVDGGAAANNLLLQFLSDMTGSRVERPINTETTAAGAAYLAGLAIDFWTDQEEVLKIRKVDREFNPDMSGEKRE
QLYRGWKKAINAALSWSKT
>Mature_499_residues
MNKKYIMAIDQGTTSTRAMIFNHRGEVVSKSQQEFSQIYPKPGWVEHSPDEIWVSILGVMSGALRSKGISPKEIAAIGIT
NQRETTVIWDKNTDEPVYNAIVWQCRRTTDICQELKNNGLEDKFKEKTGLVLDPYFSGTKIKWILDNVAGARQKAKNGDL
LFGTIDSWLIWKLTGGKVHVTDYTNASRTLLYNIHELDWDEELLDILGIPESILPEVKASSTIYGNTVPYHFFGEEVPIS
GIAGDQQAATFAQGCYEKGMTKITYGTGGFMLMNTGKEAVTSDNGLLTTIAWGLDDGIYYALEGSIFNAGSAIQWLRDEL
DLIEDAADSEYFASKVEDTGGVYVVPAFTGLGAPYWDPRARGIIVGLTRGTNKNHLIRATIESLIYQSKDVLMAMVEDSG
LDLKDIKVDGGAAANNLLLQFLSDMTGSRVERPINTETTAAGAAYLAGLAIDFWTDQEEVLKIRKVDREFNPDMSGEKRE
QLYRGWKKAINAALSWSKT

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI42794763, Length=509, Percent_Identity=45.7760314341847, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI189409120, Length=505, Percent_Identity=45.9405940594059, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI4504007, Length=505, Percent_Identity=45.9405940594059, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=45.6175298804781, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=33.1372549019608, Blast_Score=334, Evalue=1e-91,
Organism=Escherichia coli, GI1790361, Length=496, Percent_Identity=61.6935483870968, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=494, Percent_Identity=24.8987854251012, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1787789, Length=506, Percent_Identity=26.2845849802372, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1789987, Length=479, Percent_Identity=22.7557411273486, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1789138, Length=499, Percent_Identity=22.4448897795591, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535599, Length=507, Percent_Identity=40.6311637080868, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=24.5681381957774, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71986147, Length=299, Percent_Identity=29.4314381270903, Blast_Score=141, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6321755, Length=549, Percent_Identity=34.4262295081967, Blast_Score=305, Evalue=9e-84,
Organism=Drosophila melanogaster, GI17864214, Length=510, Percent_Identity=43.3333333333333, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24654573, Length=435, Percent_Identity=45.2873563218391, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655555, Length=498, Percent_Identity=38.9558232931727, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24655560, Length=498, Percent_Identity=38.9558232931727, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24655570, Length=498, Percent_Identity=38.9558232931727, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24655574, Length=498, Percent_Identity=38.9558232931727, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24655565, Length=498, Percent_Identity=38.9558232931727, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=33.2068311195446, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24652871, Length=495, Percent_Identity=33.5353535353535, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24656367, Length=489, Percent_Identity=30.0613496932515, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI21357867, Length=500, Percent_Identity=28.6, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24656372, Length=324, Percent_Identity=32.7160493827161, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=29.3103448275862, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_HALOH (B8CW97)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508561.1
- ProteinModelPortal:   B8CW97
- SMR:   B8CW97
- GeneID:   7314796
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_08090
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55295; Mature: 55295

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 135-135 BINDING 245-245 BINDING 267-267 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKYIMAIDQGTTSTRAMIFNHRGEVVSKSQQEFSQIYPKPGWVEHSPDEIWVSILGVM
CCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
SGALRSKGISPKEIAAIGITNQRETTVIWDKNTDEPVYNAIVWQCRRTTDICQELKNNGL
HHHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCC
EDKFKEKTGLVLDPYFSGTKIKWILDNVAGARQKAKNGDLLFGTIDSWLIWKLTGGKVHV
CHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCEEEE
TDYTNASRTLLYNIHELDWDEELLDILGIPESILPEVKASSTIYGNTVPYHFFGEEVPIS
EECCCCCCEEEEEEHHCCCHHHHHHHHCCCHHHHCCCCCCCEEECCCCCEEECCCCCCCC
GIAGDQQAATFAQGCYEKGMTKITYGTGGFMLMNTGKEAVTSDNGLLTTIAWGLDDGIYY
CCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHCCCCCEEEEEEECCCCCEEE
ALEGSIFNAGSAIQWLRDELDLIEDAADSEYFASKVEDTGGVYVVPAFTGLGAPYWDPRA
EEECCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
RGIIVGLTRGTNKNHLIRATIESLIYQSKDVLMAMVEDSGLDLKDIKVDGGAAANNLLLQ
CEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHH
FLSDMTGSRVERPINTETTAAGAAYLAGLAIDFWTDQEEVLKIRKVDREFNPDMSGEKRE
HHHHHCCHHHCCCCCCCCHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHH
QLYRGWKKAINAALSWSKT
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKKYIMAIDQGTTSTRAMIFNHRGEVVSKSQQEFSQIYPKPGWVEHSPDEIWVSILGVM
CCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
SGALRSKGISPKEIAAIGITNQRETTVIWDKNTDEPVYNAIVWQCRRTTDICQELKNNGL
HHHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCC
EDKFKEKTGLVLDPYFSGTKIKWILDNVAGARQKAKNGDLLFGTIDSWLIWKLTGGKVHV
CHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCEEEE
TDYTNASRTLLYNIHELDWDEELLDILGIPESILPEVKASSTIYGNTVPYHFFGEEVPIS
EECCCCCCEEEEEEHHCCCHHHHHHHHCCCHHHHCCCCCCCEEECCCCCEEECCCCCCCC
GIAGDQQAATFAQGCYEKGMTKITYGTGGFMLMNTGKEAVTSDNGLLTTIAWGLDDGIYY
CCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHCCCCCEEEEEEECCCCCEEE
ALEGSIFNAGSAIQWLRDELDLIEDAADSEYFASKVEDTGGVYVVPAFTGLGAPYWDPRA
EEECCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
RGIIVGLTRGTNKNHLIRATIESLIYQSKDVLMAMVEDSGLDLKDIKVDGGAAANNLLLQ
CEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHH
FLSDMTGSRVERPINTETTAAGAAYLAGLAIDFWTDQEEVLKIRKVDREFNPDMSGEKRE
HHHHHCCHHHCCCCCCCCHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHH
QLYRGWKKAINAALSWSKT
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA