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The map label for this gene is aegA [H]

Identifier: 220931491

GI number: 220931491

Start: 701631

End: 703061

Strand: Direct

Name: aegA [H]

Synonym: Hore_06470

Alternate gene names: 220931491

Gene position: 701631-703061 (Clockwise)

Preceding gene: 220931490

Following gene: 220931492

Centisome position: 27.21

GC content: 40.95

Gene sequence:

>1431_bases
ATGCCAGTAGTCAGAAAACGGGTTCCAATGAAAAATCAACCTCCTGATGAAAGAATAAAAAATTTTAAAGAGGTTCCCCT
TGGTTATAATAAAAAAGAAGCCATTAAAGAAGCCGAGCGCTGCCTTCAATGTAAACACTCACCCTGTGTTTCAGGTTGTC
CGGTGAATGTACCTATCCCCCGGTTTATAAAAGCCTTAAGAGAAAATGATATTAAAGGGGCAAACAGAATAATAAAGTCT
AAAAATAATTTACCGGCAATCTGTGGCCGGGTCTGTCCCCAGGAAAACCAGTGTGAAAAAGTATGTGTTTTGGGTCAAAA
ATTTGAACCGGTAGCCATCGGTAGGCTGGAAAGATTTGTGGCTGATTTAATACTTGAGCAAGAGAAAAATAATAATTCAG
GAGAAAAGACTGTAACCGACGGAGATAATATTTCTACTAATTCTGATTCCAGAGACAGGGTTGCGGTGGTTGGCTCAGGA
CCGGCAGGACTTACTGCTGCAGCCGATCTGGCTAAAGAAGGTTTTAAAGTAACAGTTTTTGAAGCCCTGCATGATACCGG
TGGTGTTCTTCGTTATGGTATTCCTGAATTTAGATTGCCAAAGTCTATTTTAGACCAGGAAGTTGAAAATATAAAAAAAC
TCGGTGTGGAATTTAAATTAAATGTAATAATCGGAAAAACAATAACAATTGATGAATTATTTGAAGACGGGTATAAAGCT
GTTTTTATTGGAACTGGTGCTGGATTGCCCCTATTTTTAAATATTCCTGGTGAAAATCTTAATGGGGTTTATTCAGCCAA
TGAATTTTTAACCAGGGTTAACCTGATGAAGGCCTATAAATTTCCTGAGTTCAAAACACCGGTTAAGGTTGGCAGGAGGG
TTGCGGTAATTGGTGGTGGAAATGTGGCTATGGATGCTGCCAGAACAGCAAAAAGGCTCGGAGCTGAAAAGGTAATGGTT
GTGTACCGGCGTTCAGAAGAGGAAATGCCTGCCCGGAATGAAGAAATTGAGCATGCCAGGGAAGAGGGGATTGAATTTAT
ATTATTGAGCAGTCCGGTTAAAATTCATGGGGAGAACGGTCTGGTAACTGCTCTCGAATGTATTAAAATGGAACTTGGTG
AAAAAGATAAATCAGGACGGAGGCGACCCATTCCGGTTGAGGGTTCTAACTGGAACATGAAATTGGATACTGTTATTGTT
GCCATTGGTCAGACTCCCAATCCTTTATTAACCAGTAATTCCCGTGGAATTGAGACTGAATCCTGGGGTGGTATTAAAGT
TGATGAAAATCTATCCACTTCACGTCAGGGTGTTTTTGCCGGTGGTGATGTAGTTACCGGGGCGGCTACAGTTATCGAAG
CTATGGGGGCTGGAAAGAAAGCGGCCCGTAGTATTTTAGAGTATTTGCGTAGCGATAATAAAGATAAGTAA

Upstream 100 bases:

>100_bases
CCTTTGATGGTCACCAGGTTGATTTTGAGGAGTTAATGAATAGACTTAACTTTTATAACGAGGAAGAAAAAATGGTCAAC
AAAAAAGAGGTGAGTGACTG

Downstream 100 bases:

>100_bases
AGGGGGAGATTTTTAAGTGGGTAAGATTATTGATGGGAAGAAAATTGCCAGTGAGATCAGGAATGAATTGAAGGAAGAAG
TAAAAAAATTTACTGAAGAG

Product: glutamate synthase (NADPH) small subunit

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MPVVRKRVPMKNQPPDERIKNFKEVPLGYNKKEAIKEAERCLQCKHSPCVSGCPVNVPIPRFIKALRENDIKGANRIIKS
KNNLPAICGRVCPQENQCEKVCVLGQKFEPVAIGRLERFVADLILEQEKNNNSGEKTVTDGDNISTNSDSRDRVAVVGSG
PAGLTAAADLAKEGFKVTVFEALHDTGGVLRYGIPEFRLPKSILDQEVENIKKLGVEFKLNVIIGKTITIDELFEDGYKA
VFIGTGAGLPLFLNIPGENLNGVYSANEFLTRVNLMKAYKFPEFKTPVKVGRRVAVIGGGNVAMDAARTAKRLGAEKVMV
VYRRSEEEMPARNEEIEHAREEGIEFILLSSPVKIHGENGLVTALECIKMELGEKDKSGRRRPIPVEGSNWNMKLDTVIV
AIGQTPNPLLTSNSRGIETESWGGIKVDENLSTSRQGVFAGGDVVTGAATVIEAMGAGKKAARSILEYLRSDNKDK

Sequences:

>Translated_476_residues
MPVVRKRVPMKNQPPDERIKNFKEVPLGYNKKEAIKEAERCLQCKHSPCVSGCPVNVPIPRFIKALRENDIKGANRIIKS
KNNLPAICGRVCPQENQCEKVCVLGQKFEPVAIGRLERFVADLILEQEKNNNSGEKTVTDGDNISTNSDSRDRVAVVGSG
PAGLTAAADLAKEGFKVTVFEALHDTGGVLRYGIPEFRLPKSILDQEVENIKKLGVEFKLNVIIGKTITIDELFEDGYKA
VFIGTGAGLPLFLNIPGENLNGVYSANEFLTRVNLMKAYKFPEFKTPVKVGRRVAVIGGGNVAMDAARTAKRLGAEKVMV
VYRRSEEEMPARNEEIEHAREEGIEFILLSSPVKIHGENGLVTALECIKMELGEKDKSGRRRPIPVEGSNWNMKLDTVIV
AIGQTPNPLLTSNSRGIETESWGGIKVDENLSTSRQGVFAGGDVVTGAATVIEAMGAGKKAARSILEYLRSDNKDK
>Mature_475_residues
PVVRKRVPMKNQPPDERIKNFKEVPLGYNKKEAIKEAERCLQCKHSPCVSGCPVNVPIPRFIKALRENDIKGANRIIKSK
NNLPAICGRVCPQENQCEKVCVLGQKFEPVAIGRLERFVADLILEQEKNNNSGEKTVTDGDNISTNSDSRDRVAVVGSGP
AGLTAAADLAKEGFKVTVFEALHDTGGVLRYGIPEFRLPKSILDQEVENIKKLGVEFKLNVIIGKTITIDELFEDGYKAV
FIGTGAGLPLFLNIPGENLNGVYSANEFLTRVNLMKAYKFPEFKTPVKVGRRVAVIGGGNVAMDAARTAKRLGAEKVMVV
YRRSEEEMPARNEEIEHAREEGIEFILLSSPVKIHGENGLVTALECIKMELGEKDKSGRRRPIPVEGSNWNMKLDTVIVA
IGQTPNPLLTSNSRGIETESWGGIKVDENLSTSRQGVFAGGDVVTGAATVIEAMGAGKKAARSILEYLRSDNKDK

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=488, Percent_Identity=32.9918032786885, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI237757300, Length=125, Percent_Identity=36, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI111118983, Length=165, Percent_Identity=32.1212121212121, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI111118981, Length=165, Percent_Identity=32.1212121212121, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788811, Length=486, Percent_Identity=37.4485596707819, Blast_Score=311, Evalue=8e-86,
Organism=Escherichia coli, GI87082180, Length=458, Percent_Identity=37.7729257641921, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1789606, Length=472, Percent_Identity=36.4406779661017, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788468, Length=452, Percent_Identity=34.2920353982301, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1789243, Length=469, Percent_Identity=32.6226012793177, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71984108, Length=453, Percent_Identity=34.2163355408389, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17570289, Length=491, Percent_Identity=28.5132382892057, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17543860, Length=160, Percent_Identity=38.125, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=492, Percent_Identity=29.8780487804878, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6320584, Length=176, Percent_Identity=34.0909090909091, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640763, Length=462, Percent_Identity=30.0865800865801, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI18858217, Length=462, Percent_Identity=30.0865800865801, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24665547, Length=490, Percent_Identity=29.3877551020408, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24665543, Length=490, Percent_Identity=29.3877551020408, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=29.3877551020408, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=29.3877551020408, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17137178, Length=159, Percent_Identity=31.4465408805031, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 52124; Mature: 51992

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVRKRVPMKNQPPDERIKNFKEVPLGYNKKEAIKEAERCLQCKHSPCVSGCPVNVPIP
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
RFIKALRENDIKGANRIIKSKNNLPAICGRVCPQENQCEKVCVLGQKFEPVAIGRLERFV
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHH
ADLILEQEKNNNSGEKTVTDGDNISTNSDSRDRVAVVGSGPAGLTAAADLAKEGFKVTVF
HHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEE
EALHDTGGVLRYGIPEFRLPKSILDQEVENIKKLGVEFKLNVIIGKTITIDELFEDGYKA
EHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCEEEHHHHHHCCCEE
VFIGTGAGLPLFLNIPGENLNGVYSANEFLTRVNLMKAYKFPEFKTPVKVGRRVAVIGGG
EEEECCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCEEEEEECC
NVAMDAARTAKRLGAEKVMVVYRRSEEEMPARNEEIEHAREEGIEFILLSSPVKIHGENG
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEECCCC
LVTALECIKMELGEKDKSGRRRPIPVEGSNWNMKLDTVIVAIGQTPNPLLTSNSRGIETE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCC
SWGGIKVDENLSTSRQGVFAGGDVVTGAATVIEAMGAGKKAARSILEYLRSDNKDK
CCCCEEECCCCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PVVRKRVPMKNQPPDERIKNFKEVPLGYNKKEAIKEAERCLQCKHSPCVSGCPVNVPIP
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
RFIKALRENDIKGANRIIKSKNNLPAICGRVCPQENQCEKVCVLGQKFEPVAIGRLERFV
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHH
ADLILEQEKNNNSGEKTVTDGDNISTNSDSRDRVAVVGSGPAGLTAAADLAKEGFKVTVF
HHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEE
EALHDTGGVLRYGIPEFRLPKSILDQEVENIKKLGVEFKLNVIIGKTITIDELFEDGYKA
EHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCEEEHHHHHHCCCEE
VFIGTGAGLPLFLNIPGENLNGVYSANEFLTRVNLMKAYKFPEFKTPVKVGRRVAVIGGG
EEEECCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCEEEEEECC
NVAMDAARTAKRLGAEKVMVVYRRSEEEMPARNEEIEHAREEGIEFILLSSPVKIHGENG
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEECCCC
LVTALECIKMELGEKDKSGRRRPIPVEGSNWNMKLDTVIVAIGQTPNPLLTSNSRGIETE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCC
SWGGIKVDENLSTSRQGVFAGGDVVTGAATVIEAMGAGKKAARSILEYLRSDNKDK
CCCCEEECCCCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]