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The map label for this gene is folD

Identifier: 220931492

GI number: 220931492

Start: 703078

End: 703929

Strand: Direct

Name: folD

Synonym: Hore_06480

Alternate gene names: 220931492

Gene position: 703078-703929 (Clockwise)

Preceding gene: 220931491

Following gene: 220931493

Centisome position: 27.27

GC content: 44.25

Gene sequence:

>852_bases
GTGGGTAAGATTATTGATGGGAAGAAAATTGCCAGTGAGATCAGGAATGAATTGAAGGAAGAAGTAAAAAAATTTACTGA
AGAGGGGCGCCCTCCAGGACTGTCAGTAGTTCTGGTTGGAGATGATCCTGCGTCCCGGACCTATGTCAGAATGAAAGAGA
AGGTGGCCAAAGAAATTGGTATATATTCAAAAGTGGATTACCTCTCCAGGCAGACAACTCAGGATGAGTTGCTTGATATT
GTCGACAAATTAAATAATGATGAAAAAATTGATGGTATACTGGTTCAGTTACCTTTACCTGATCATATCGATGAAAAAGC
TGTGATAGAAGCTATTAGTCCTTTTAAAGATGTGGACGGTTTTCATCCTATAAATACAGGAAAACTATTTAGTGGGATGA
CCGGGCAGCGGTTTGAGGCCTGTACCCCCCTCGGTATTATTGAGCTACTTGACAGGGAAGGAATAGAAATAGATGGGAAA
AAGGCAGTTGTAGTAGGAAGGAGTAATATTGTAGGTAAACCGGTGGCCCATTTATTACTGGAAAGACATGCTACTGTTAC
AGTTTGCCATTCCCATACCGCAGATCTGGGTATTGAAACCAGACAGGCAGATATACTGGTAGTTGCAGCCGGTCGTCCCA
AACTTGTCAAGGGCGAAATGGTCAAGGAGGGTGCTGCTGTAATTGATGTGGGGACCAACCGGGTTGATGGTCATCTGGTC
GGGGATGTTGATTTTGAAGCAGCCCGCCAGAGGGCTGGTTACATAACTCCGGTGCCCGGAGGAGTAGGTCCGATGACCAT
AGCCATGCTTATGAAAAATACTGTTAAAGCCAGGAAGTATCATGGAGTCTAA

Upstream 100 bases:

>100_bases
ACAGTTATCGAAGCTATGGGGGCTGGAAAGAAAGCGGCCCGTAGTATTTTAGAGTATTTGCGTAGCGATAATAAAGATAA
GTAAAGGGGGAGATTTTTAA

Downstream 100 bases:

>100_bases
AATATACACTGTATCTGAAGTTACCGGTCGAATAACAGAATTATTAACTTCGGATTCTGTTTTAAATGACCTCTGGATAA
CCGGGGAAATATCAAATTTC

Product: tetrahydrofolate dehydrogenase/cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MGKIIDGKKIASEIRNELKEEVKKFTEEGRPPGLSVVLVGDDPASRTYVRMKEKVAKEIGIYSKVDYLSRQTTQDELLDI
VDKLNNDEKIDGILVQLPLPDHIDEKAVIEAISPFKDVDGFHPINTGKLFSGMTGQRFEACTPLGIIELLDREGIEIDGK
KAVVVGRSNIVGKPVAHLLLERHATVTVCHSHTADLGIETRQADILVVAAGRPKLVKGEMVKEGAAVIDVGTNRVDGHLV
GDVDFEAARQRAGYITPVPGGVGPMTIAMLMKNTVKARKYHGV

Sequences:

>Translated_283_residues
MGKIIDGKKIASEIRNELKEEVKKFTEEGRPPGLSVVLVGDDPASRTYVRMKEKVAKEIGIYSKVDYLSRQTTQDELLDI
VDKLNNDEKIDGILVQLPLPDHIDEKAVIEAISPFKDVDGFHPINTGKLFSGMTGQRFEACTPLGIIELLDREGIEIDGK
KAVVVGRSNIVGKPVAHLLLERHATVTVCHSHTADLGIETRQADILVVAAGRPKLVKGEMVKEGAAVIDVGTNRVDGHLV
GDVDFEAARQRAGYITPVPGGVGPMTIAMLMKNTVKARKYHGV
>Mature_282_residues
GKIIDGKKIASEIRNELKEEVKKFTEEGRPPGLSVVLVGDDPASRTYVRMKEKVAKEIGIYSKVDYLSRQTTQDELLDIV
DKLNNDEKIDGILVQLPLPDHIDEKAVIEAISPFKDVDGFHPINTGKLFSGMTGQRFEACTPLGIIELLDREGIEIDGKK
AVVVGRSNIVGKPVAHLLLERHATVTVCHSHTADLGIETRQADILVVAAGRPKLVKGEMVKEGAAVIDVGTNRVDGHLVG
DVDFEAARQRAGYITPVPGGVGPMTIAMLMKNTVKARKYHGV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=50.1706484641638, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=48.0968858131488, Blast_Score=248, Evalue=3e-66,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=49.8293515358362, Blast_Score=247, Evalue=9e-66,
Organism=Homo sapiens, GI36796743, Length=222, Percent_Identity=27.027027027027, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=51.5901060070671, Blast_Score=270, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=42.6116838487972, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=45.3287197231834, Blast_Score=249, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=295, Percent_Identity=45.7627118644068, Blast_Score=239, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6322933, Length=319, Percent_Identity=25.705329153605, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=49.3243243243243, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=49.3243243243243, Blast_Score=255, Evalue=2e-68,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=50.5226480836237, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=50.5226480836237, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=50.5226480836237, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=50.5226480836237, Blast_Score=249, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_HALOH (B8D2H8)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508400.1
- ProteinModelPortal:   B8D2H8
- SMR:   B8D2H8
- GeneID:   7314554
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_06480
- HOGENOM:   HBG328751
- OMA:   GIDNTKV
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30801; Mature: 30670

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIIDGKKIASEIRNELKEEVKKFTEEGRPPGLSVVLVGDDPASRTYVRMKEKVAKEIG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHC
IYSKVDYLSRQTTQDELLDIVDKLNNDEKIDGILVQLPLPDHIDEKAVIEAISPFKDVDG
CHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHCCC
FHPINTGKLFSGMTGQRFEACTPLGIIELLDREGIEIDGKKAVVVGRSNIVGKPVAHLLL
CCCCCCCHHHCCCCCCCHHCCCCHHHHHHHCCCCCEECCCEEEEEECCCCCCHHHHHHHH
ERHATVTVCHSHTADLGIETRQADILVVAAGRPKLVKGEMVKEGAAVIDVGTNRVDGHLV
HCCCEEEEEECCCCCCCCEECCCCEEEEECCCCCEECCHHHHCCCEEEEECCCCCCCEEE
GDVDFEAARQRAGYITPVPGGVGPMTIAMLMKNTVKARKYHGV
ECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GKIIDGKKIASEIRNELKEEVKKFTEEGRPPGLSVVLVGDDPASRTYVRMKEKVAKEIG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHC
IYSKVDYLSRQTTQDELLDIVDKLNNDEKIDGILVQLPLPDHIDEKAVIEAISPFKDVDG
CHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHCCC
FHPINTGKLFSGMTGQRFEACTPLGIIELLDREGIEIDGKKAVVVGRSNIVGKPVAHLLL
CCCCCCCHHHCCCCCCCHHCCCCHHHHHHHCCCCCEECCCEEEEEECCCCCCHHHHHHHH
ERHATVTVCHSHTADLGIETRQADILVVAAGRPKLVKGEMVKEGAAVIDVGTNRVDGHLV
HCCCEEEEEECCCCCCCCEECCCCEEEEECCCCCEECCHHHHCCCEEEEECCCCCCCEEE
GDVDFEAARQRAGYITPVPGGVGPMTIAMLMKNTVKARKYHGV
ECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA