Click here to switch to the map view.

The map label for this gene is metG

Identifier: 220931388

GI number: 220931388

Start: 591733

End: 593355

Strand: Direct

Name: metG

Synonym: Hore_05440

Alternate gene names: 220931388

Gene position: 591733-593355 (Clockwise)

Preceding gene: 220931385

Following gene: 220931390

Centisome position: 22.95

GC content: 37.95

Gene sequence:

>1623_bases
ATGAATGTATTTATTGGTGGGGCCTGGCCCTATGCTAACGGTTCTTTACATCTGGGTCATATAGCCAGTCTTTTGCCCGG
TGACATTCTGGCCAGATATTTTCGACTTAAGGGGGAAAAAGTCCTGTATGTGTCAGGGAGTGATTGTCATGGAACCCCGA
TTGCCCTTAGGGCAAAAAGGGAAGGGGAGAGTCCCCGGATTATAGCTGAAAGATATCATCAGGAGTTTAAAAAAACCTTT
ACAAAGCTGGGTTTTTCTTACGATAATTATACGAAGACTGATAACCCCTTTCATCACCAGGTGGTACAGAGTATAATAAA
AAAACTATTTGAAAAGGGGTACATTTATAAAAAGCAAACCAAACAGCTTTATTGTAGTAAATGTCAACAGTTTTTACCTG
ATAGGTATGTGGAAGGAATTTGCCCTGTATGTGGCAAAGTCGTCCGAGGTGATCAATGCGATTACTGTTCCAGTTTAATT
GAGCCCACTGATCTGGAAGAAAAGAGATGTAAAATATGTGGGAGTAGTCCAGTCATTAAAAATACAGAGCACTTCTATCT
GGCCCTGTCAGAATTTAGTGATGAATTAAAGAGGTTACTTGATAATAATCGGGGGTGGAGAGATAATGCAATTAAGCTCA
GTACCAGGTATATAGAGGAAGGTCTACAGGACAGGGCCATAACCAGGGACCTCAACTGGGGTGTTTCTATACCTTTTGAA
GGCTATGAAGAGAAAAGAATATATGTCTGGATTGATGCTGTGTTAGGTTATCTGTCTGCCAGTAAGGAATGGTCCCGGCA
TACAGGGGAACCCTGGCAAAATTTTTGGAGTGAGGATACAAAAACATATTATGTTCATGGAAAAGATAATATACCTTTTC
ACACCCTGATACTACCTGCGTTACTCCGGGGTGCTGGAATTAAACAAATTCCAGAAAAAATAGTTTCCAGTGAATACCTT
ACAATTGAGGGGAAGAAATTGTCAACCAGTCGTAATTGGGCTGTCTGGCTTCCTGATATGTTAAAAAATTACCAGCCTGA
TTCTATTCGTTACTTTTTGATAATAAATGGTCCCGAGAAGAAAGACAGTAATTTTTCCTGGGAAGAGTTTATTCACAGCC
ATAATGGGGAACTTTTGGGGGCATATGGTAATTTTGTTAACCGGACTCTTGTTTTTATTGAAAAATATTACAATGGTTTA
ATACCTGATGGTAAACTAAATGAAAAAATTAAGGAAAAAATTGAGACCCTCTACCATAGGGTGGGCAATAAGATAGAGAA
GACGAATTTAAAAAATGCCCTGGAGGTTATATTTAATTTTGTACGTGAAGCTAATAAGTATTTTGATAGTGAGGAGCCAT
GGATTACTGTTAAAGAAGACAAAGGTACCTGTAAAGATACAATCTATACGTGTGTTCAAATAATTGCAAATTTAACAAAT
TTATTGTCCCCATTCCTCCCTTTTTCCAGCAAAAAAGTCAGGGGTTTTTTAAACATTGATAAACCTGACTGGAAATATAT
AGAGGTTTTGCCTGCAACAAAGTTAGAAAAAGTAGAAATTCTTTTCCCGAGAATTAACAAAAACCTAATTAAGGAGGAAC
GGGAAAAGTTAGGGGTTAATTAG

Upstream 100 bases:

>100_bases
TTAACTGTCTTAATCGGAGTGTTTATTATGTAAAATCTTCAATCTATAAGAGGGTTAAGTGTAATATTATAAGATATATT
AAAGGGGGAGTGTTAGACAA

Downstream 100 bases:

>100_bases
TAATAAAACGGTTTATGGGAAAATAGTCAAAAAAGAAATGTGGATTTTAGGACTTGCAAATATTTGTGTATATATGGTTA
ACAAAATTATTAAAGAGTTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MNVFIGGAWPYANGSLHLGHIASLLPGDILARYFRLKGEKVLYVSGSDCHGTPIALRAKREGESPRIIAERYHQEFKKTF
TKLGFSYDNYTKTDNPFHHQVVQSIIKKLFEKGYIYKKQTKQLYCSKCQQFLPDRYVEGICPVCGKVVRGDQCDYCSSLI
EPTDLEEKRCKICGSSPVIKNTEHFYLALSEFSDELKRLLDNNRGWRDNAIKLSTRYIEEGLQDRAITRDLNWGVSIPFE
GYEEKRIYVWIDAVLGYLSASKEWSRHTGEPWQNFWSEDTKTYYVHGKDNIPFHTLILPALLRGAGIKQIPEKIVSSEYL
TIEGKKLSTSRNWAVWLPDMLKNYQPDSIRYFLIINGPEKKDSNFSWEEFIHSHNGELLGAYGNFVNRTLVFIEKYYNGL
IPDGKLNEKIKEKIETLYHRVGNKIEKTNLKNALEVIFNFVREANKYFDSEEPWITVKEDKGTCKDTIYTCVQIIANLTN
LLSPFLPFSSKKVRGFLNIDKPDWKYIEVLPATKLEKVEILFPRINKNLIKEEREKLGVN

Sequences:

>Translated_540_residues
MNVFIGGAWPYANGSLHLGHIASLLPGDILARYFRLKGEKVLYVSGSDCHGTPIALRAKREGESPRIIAERYHQEFKKTF
TKLGFSYDNYTKTDNPFHHQVVQSIIKKLFEKGYIYKKQTKQLYCSKCQQFLPDRYVEGICPVCGKVVRGDQCDYCSSLI
EPTDLEEKRCKICGSSPVIKNTEHFYLALSEFSDELKRLLDNNRGWRDNAIKLSTRYIEEGLQDRAITRDLNWGVSIPFE
GYEEKRIYVWIDAVLGYLSASKEWSRHTGEPWQNFWSEDTKTYYVHGKDNIPFHTLILPALLRGAGIKQIPEKIVSSEYL
TIEGKKLSTSRNWAVWLPDMLKNYQPDSIRYFLIINGPEKKDSNFSWEEFIHSHNGELLGAYGNFVNRTLVFIEKYYNGL
IPDGKLNEKIKEKIETLYHRVGNKIEKTNLKNALEVIFNFVREANKYFDSEEPWITVKEDKGTCKDTIYTCVQIIANLTN
LLSPFLPFSSKKVRGFLNIDKPDWKYIEVLPATKLEKVEILFPRINKNLIKEEREKLGVN
>Mature_540_residues
MNVFIGGAWPYANGSLHLGHIASLLPGDILARYFRLKGEKVLYVSGSDCHGTPIALRAKREGESPRIIAERYHQEFKKTF
TKLGFSYDNYTKTDNPFHHQVVQSIIKKLFEKGYIYKKQTKQLYCSKCQQFLPDRYVEGICPVCGKVVRGDQCDYCSSLI
EPTDLEEKRCKICGSSPVIKNTEHFYLALSEFSDELKRLLDNNRGWRDNAIKLSTRYIEEGLQDRAITRDLNWGVSIPFE
GYEEKRIYVWIDAVLGYLSASKEWSRHTGEPWQNFWSEDTKTYYVHGKDNIPFHTLILPALLRGAGIKQIPEKIVSSEYL
TIEGKKLSTSRNWAVWLPDMLKNYQPDSIRYFLIINGPEKKDSNFSWEEFIHSHNGELLGAYGNFVNRTLVFIEKYYNGL
IPDGKLNEKIKEKIETLYHRVGNKIEKTNLKNALEVIFNFVREANKYFDSEEPWITVKEDKGTCKDTIYTCVQIIANLTN
LLSPFLPFSSKKVRGFLNIDKPDWKYIEVLPATKLEKVEILFPRINKNLIKEEREKLGVN

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=559, Percent_Identity=32.737030411449, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI24308436, Length=576, Percent_Identity=25.8680555555556, Blast_Score=159, Evalue=5e-39,
Organism=Escherichia coli, GI1788432, Length=540, Percent_Identity=29.6296296296296, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1786861, Length=227, Percent_Identity=25.9911894273128, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=561, Percent_Identity=31.729055258467, Blast_Score=325, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI212645096, Length=509, Percent_Identity=26.3261296660118, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71992781, Length=401, Percent_Identity=25.4364089775561, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321704, Length=557, Percent_Identity=31.9569120287253, Blast_Score=302, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6321610, Length=542, Percent_Identity=24.7232472324723, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24655311, Length=559, Percent_Identity=34.8837209302326, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI21358483, Length=521, Percent_Identity=22.4568138195777, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_HALOH (B8D274)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508296.1
- GeneID:   7313508
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_05440
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- ProtClustDB:   PRK12268
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 62658; Mature: 62658

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVFIGGAWPYANGSLHLGHIASLLPGDILARYFRLKGEKVLYVSGSDCHGTPIALRAKR
CCEEEECCCCCCCCCEEHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECC
EGESPRIIAERYHQEFKKTFTKLGFSYDNYTKTDNPFHHQVVQSIIKKLFEKGYIYKKQT
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHH
KQLYCSKCQQFLPDRYVEGICPVCGKVVRGDQCDYCSSLIEPTDLEEKRCKICGSSPVIK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHCCCCCCCC
NTEHFYLALSEFSDELKRLLDNNRGWRDNAIKLSTRYIEEGLQDRAITRDLNWGVSIPFE
CCCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCHHHHHHHCCCCCCEECCCC
GYEEKRIYVWIDAVLGYLSASKEWSRHTGEPWQNFWSEDTKTYYVHGKDNIPFHTLILPA
CCCCCEEEEEHHHHHHHHHCCHHHHHCCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
LLRGAGIKQIPEKIVSSEYLTIEGKKLSTSRNWAVWLPDMLKNYQPDSIRYFLIINGPEK
HHHCCCHHHHHHHHHCCCEEEEECCEECCCCCEEEECHHHHCCCCCCCEEEEEEEECCCC
KDSNFSWEEFIHSHNGELLGAYGNFVNRTLVFIEKYYNGLIPDGKLNEKIKEKIETLYHR
CCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
VGNKIEKTNLKNALEVIFNFVREANKYFDSEEPWITVKEDKGTCKDTIYTCVQIIANLTN
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
LLSPFLPFSSKKVRGFLNIDKPDWKYIEVLPATKLEKVEILFPRINKNLIKEEREKLGVN
HHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNVFIGGAWPYANGSLHLGHIASLLPGDILARYFRLKGEKVLYVSGSDCHGTPIALRAKR
CCEEEECCCCCCCCCEEHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECC
EGESPRIIAERYHQEFKKTFTKLGFSYDNYTKTDNPFHHQVVQSIIKKLFEKGYIYKKQT
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHH
KQLYCSKCQQFLPDRYVEGICPVCGKVVRGDQCDYCSSLIEPTDLEEKRCKICGSSPVIK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHCCCCCCCC
NTEHFYLALSEFSDELKRLLDNNRGWRDNAIKLSTRYIEEGLQDRAITRDLNWGVSIPFE
CCCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCHHHHHHHCCCCCCEECCCC
GYEEKRIYVWIDAVLGYLSASKEWSRHTGEPWQNFWSEDTKTYYVHGKDNIPFHTLILPA
CCCCCEEEEEHHHHHHHHHCCHHHHHCCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
LLRGAGIKQIPEKIVSSEYLTIEGKKLSTSRNWAVWLPDMLKNYQPDSIRYFLIINGPEK
HHHCCCHHHHHHHHHCCCEEEEECCEECCCCCEEEECHHHHCCCCCCCEEEEEEEECCCC
KDSNFSWEEFIHSHNGELLGAYGNFVNRTLVFIEKYYNGLIPDGKLNEKIKEKIETLYHR
CCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
VGNKIEKTNLKNALEVIFNFVREANKYFDSEEPWITVKEDKGTCKDTIYTCVQIIANLTN
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
LLSPFLPFSSKKVRGFLNIDKPDWKYIEVLPATKLEKVEILFPRINKNLIKEEREKLGVN
HHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA