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The map label for this gene is purR [H]

Identifier: 220931302

GI number: 220931302

Start: 490264

End: 491271

Strand: Direct

Name: purR [H]

Synonym: Hore_04570

Alternate gene names: 220931302

Gene position: 490264-491271 (Clockwise)

Preceding gene: 220931301

Following gene: 220931303

Centisome position: 19.02

GC content: 34.52

Gene sequence:

>1008_bases
ATGCCTGTTACTATTAAGGATATAGCTAAAAAGGCTAATGTATCCGTGACAACTGTGTCCAGGGTCTTAAATAACAAACC
CGATGTTAGTGAGAAAACTAAAAGTAAAATTCTAAAAATAATCAATGAGTTGGGCTATAATCCGAATGGAGTAGCCAGGG
GACTGGTACTAAATAGGACACATACAATTGGACTAATTATCCCTGATATAAGAAACCCATTTTTTCCTGAAGTTGCCAGA
GGTATTGAAGATAAGGCAAAAGAGTATGGTTACTCTGTTATTTATTGTAATACAGATAACAAGAAAGAGGAGGAAAAAAA
GGCTGTTGAATTATTGTTAAATAAAAGGGTGGATGGAATTATTTTATCTCTTTCTATGAATAACAGAGATGAACTGGATA
GAATAAAAAAGCAGGGTTGTCCCATTGTACAGATTGATAGGAAAGTTCCTGATGCCAATTACCCAACAGTTACAATTGAT
AATTTAATTTCAGCTTATAACGCTACTAAACATTTGATTAACCTGGGTCATAAATATATTGCTCATATTACCGGTAACCT
GGATACTAAAACCGGTCAGGATCGTCTTAACGGTTATAAAAAGGCTATGCATGAGCGAGGTATACATATAAGTCAGGAAT
GGATTATAGAGGGTGATTATAGTAAAGAAAGCGGTTATAAAAATATGAAAAGAATCTTAAATTTAAAGAATAGACCAACC
GGTATTTTTATTGCCAATGATTTAATGGCAATTGGTGCCTATAAAGCAGTATTTGAATCCAAATTAAATATACCTGAGGA
TATTTCCATAATTGGACATGATGATATTGAACTTGCTTCTCTGGTAAATCCTGAATTGACAACTATGGCTCAACCTAAAT
ATGAAATGGGCAAACACGCTGCCAGGTTACTAATTCAGGAAATTGAGGGTTCAGACCAGGATAATAACCAAAACATTCTA
TTAAATACGGAACTTGTAGTCAGGGGTTCTACAGGGGGAGTTGGTTAA

Upstream 100 bases:

>100_bases
TAATTTTTCGAAATATTTTATACTTGTAATTGGAAACGTTAACGGTAACGTTGTCGGTAACGTTAACATATGTTGTTTTA
TAGTCTGGGAGGTATAAGTA

Downstream 100 bases:

>100_bases
TAATGACAAAGGAGATTCTTGTAATAGGTAGTTTAAACATGGATTTAATTGTCCAAACTGAAAAATACCCACGTAAAGGT
GAAACAGTACCGGGAGAAAA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MPVTIKDIAKKANVSVTTVSRVLNNKPDVSEKTKSKILKIINELGYNPNGVARGLVLNRTHTIGLIIPDIRNPFFPEVAR
GIEDKAKEYGYSVIYCNTDNKKEEEKKAVELLLNKRVDGIILSLSMNNRDELDRIKKQGCPIVQIDRKVPDANYPTVTID
NLISAYNATKHLINLGHKYIAHITGNLDTKTGQDRLNGYKKAMHERGIHISQEWIIEGDYSKESGYKNMKRILNLKNRPT
GIFIANDLMAIGAYKAVFESKLNIPEDISIIGHDDIELASLVNPELTTMAQPKYEMGKHAARLLIQEIEGSDQDNNQNIL
LNTELVVRGSTGGVG

Sequences:

>Translated_335_residues
MPVTIKDIAKKANVSVTTVSRVLNNKPDVSEKTKSKILKIINELGYNPNGVARGLVLNRTHTIGLIIPDIRNPFFPEVAR
GIEDKAKEYGYSVIYCNTDNKKEEEKKAVELLLNKRVDGIILSLSMNNRDELDRIKKQGCPIVQIDRKVPDANYPTVTID
NLISAYNATKHLINLGHKYIAHITGNLDTKTGQDRLNGYKKAMHERGIHISQEWIIEGDYSKESGYKNMKRILNLKNRPT
GIFIANDLMAIGAYKAVFESKLNIPEDISIIGHDDIELASLVNPELTTMAQPKYEMGKHAARLLIQEIEGSDQDNNQNIL
LNTELVVRGSTGGVG
>Mature_334_residues
PVTIKDIAKKANVSVTTVSRVLNNKPDVSEKTKSKILKIINELGYNPNGVARGLVLNRTHTIGLIIPDIRNPFFPEVARG
IEDKAKEYGYSVIYCNTDNKKEEEKKAVELLLNKRVDGIILSLSMNNRDELDRIKKQGCPIVQIDRKVPDANYPTVTIDN
LISAYNATKHLINLGHKYIAHITGNLDTKTGQDRLNGYKKAMHERGIHISQEWIIEGDYSKESGYKNMKRILNLKNRPTG
IFIANDLMAIGAYKAVFESKLNIPEDISIIGHDDIELASLVNPELTTMAQPKYEMGKHAARLLIQEIEGSDQDNNQNILL
NTELVVRGSTGGVG

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=329, Percent_Identity=38.2978723404255, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=38.6018237082067, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=36.7469879518072, Blast_Score=227, Evalue=9e-61,
Organism=Escherichia coli, GI1789068, Length=315, Percent_Identity=33.6507936507937, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1788474, Length=308, Percent_Identity=33.7662337662338, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=32.5423728813559, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1787580, Length=334, Percent_Identity=31.437125748503, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI48994940, Length=307, Percent_Identity=30.2931596091205, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787906, Length=335, Percent_Identity=26.2686567164179, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1790715, Length=315, Percent_Identity=27.6190476190476, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786268, Length=293, Percent_Identity=25.938566552901, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789456, Length=313, Percent_Identity=28.7539936102236, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790689, Length=324, Percent_Identity=23.1481481481481, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37413; Mature: 37281

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTIKDIAKKANVSVTTVSRVLNNKPDVSEKTKSKILKIINELGYNPNGVARGLVLNRT
CCCCHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCEEEECC
HTIGLIIPDIRNPFFPEVARGIEDKAKEYGYSVIYCNTDNKKEEEKKAVELLLNKRVDGI
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEE
ILSLSMNNRDELDRIKKQGCPIVQIDRKVPDANYPTVTIDNLISAYNATKHLINLGHKYI
EEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHCHHEE
AHITGNLDTKTGQDRLNGYKKAMHERGIHISQEWIIEGDYSKESGYKNMKRILNLKNRPT
EEEECCCCCCCCHHHHHHHHHHHHHCCCEECCEEEEECCCCCCCCHHHHHHHHCCCCCCC
GIFIANDLMAIGAYKAVFESKLNIPEDISIIGHDDIELASLVNPELTTMAQPKYEMGKHA
EEEEECHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHCCCCHHHHCCCHHHHHHHH
ARLLIQEIEGSDQDNNQNILLNTELVVRGSTGGVG
HHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
PVTIKDIAKKANVSVTTVSRVLNNKPDVSEKTKSKILKIINELGYNPNGVARGLVLNRT
CCCHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCEEEECC
HTIGLIIPDIRNPFFPEVARGIEDKAKEYGYSVIYCNTDNKKEEEKKAVELLLNKRVDGI
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEE
ILSLSMNNRDELDRIKKQGCPIVQIDRKVPDANYPTVTIDNLISAYNATKHLINLGHKYI
EEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHCHHEE
AHITGNLDTKTGQDRLNGYKKAMHERGIHISQEWIIEGDYSKESGYKNMKRILNLKNRPT
EEEECCCCCCCCHHHHHHHHHHHHHCCCEECCEEEEECCCCCCCCHHHHHHHHCCCCCCC
GIFIANDLMAIGAYKAVFESKLNIPEDISIIGHDDIELASLVNPELTTMAQPKYEMGKHA
EEEEECHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHCCCCHHHHCCCHHHHHHHH
ARLLIQEIEGSDQDNNQNILLNTELVVRGSTGGVG
HHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA