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The map label for this gene is hyfB [H]

Identifier: 220918882

GI number: 220918882

Start: 4216959

End: 4218923

Strand: Direct

Name: hyfB [H]

Synonym: A2cp1_3796

Alternate gene names: 220918882

Gene position: 4216959-4218923 (Clockwise)

Preceding gene: 220918881

Following gene: 220918883

Centisome position: 83.85

GC content: 78.07

Gene sequence:

>1965_bases
GTGACCTCCGCGCTCGCCCTCGGCGCCATCGCCCTCACCGCCGCGAGCGGCCTCGCCGCGGCGCTGCCCGGCCTCCGCCC
CGCGCGCGCGCAGGCGCTCGCCACCGCGGTGCTCTGCGCCGGCGCGGCGCTGGGCCTCGCCGCGGCGGGCGTGGCGCTGG
CGGGGGGCGAGGCGGCGGCGGGCGGGGTGCTGCGGGTGGACGCGCTCTCCGGGCTGTTCCTCGTGCCCATCCTCGGCATC
GCCGCGGCGGGCTCGGCATACGGGCTGCGCTACTTCCCGCAGGCGGAGCGCGGCGCGCGCGCGGTGCGGCTGCAGCTCTT
CTACGGCCTCGCGGTCGCCGGGATGGCGCTGCTCGTGTGCGCCCCCGACGCCATCGGCTTCCTCGTCGGCTGGGAGGTGA
TGGCGCTCGCGAACTTCTTCATGGTGCAGACCGAGGACGAGGACCCGTCGGTGCGCCGCGGCGCGTTCCTGTACCTCGCC
GCCACGCACGCCGGCACCCTGGCGCTGTTCGCGCTGTTCGCGCTGCTGCGCCGCGAGGCGGGCACGTTCGAGCTCGCGGC
CATGGAGGGCCTCCGGCTCTCCGGCCCCACGGCGGCCGCGGTGCTGGCGCTGGCGCTGGCCGGCTTCGGCATGAAGGCCG
GGCTCGTGCCGCTCCACTTCTGGCTGCCCCCGGCGCACGCGGCCGCGCCCAGCCACGTGTCCGCGCTCATGTCCGGGGTG
GTGGTGAAGACCGGCATCTACGGGCTGATGCGGGTGACCGGCCTGGTGGAGTCGCCGCCCGAGTCGTGGGGCGTCGCGCT
GCTCGCGCTGGGCGGCGTCTCGGCGGTGCTGGGCGTGGCGTTCGCGCTCGCGCAGCACGACCTGAAGCGGCTGCTCGCCT
ACCACACCGTGGAGAACGTGGGGATCATCGCGCTCGGGCTCGGGCTGGCGCTGCTCGGGCGCGCCCGCGGCGTCCCGGCG
CTGGTGGTCCTCGGCCTGGGCGGCGCCGGCCTGCACGTCGTCAACCACGCGCTGTTCAAGTCGCTCCTGTTCCTCGGCGC
CGGCGCCGTACACCACGCCACCGGCACGCGCGACCTCGACCACCTGGGCGGCCTCGCGCGCGCCATGCCGCGGACGGCGC
TGCTGTTCGTGGTCGGCGCCGCGGCCATCAGCGGGCTCCCGCCGCTGAACGGCTTCGCCTCGGAGTGGCTGGTCTACCTG
GGCCTGCTCGACGGCCTGCGCCAGCCCGGGGGCGACCTGCTCGCGTACGTGACGCTGGGCGCGCCGGCGCTGGCGCTGGT
GGGCGGCCTGGCCGCGGCCTGCTTCGCGAAGGTGGTCGGGGTGGTGTTCCTGGGCAGCCCGCGCAGCCACCACGCCGACG
GCGCGCACGAGCCGCCCGCGGCCATGCTCGCGCCCATGGCGCTCCTCGCCGCCGCCTGCGCCGCCATCGGCCTGTTCCCG
GCCGCGACCACCGCGCCGCTCGCCCGCGCCGCGGCCGCCTGGGCCCGCGTCGATCCCGCGCTGCTCGCCGCCCCCGCCGC
GCGCGCGGCCGCCGGCGCCGCGCGGGTGAGCGCGGTCGCCGCGGTCCTGCTCGTGGCGACGCTGGCGGTGGCGGCGTGGC
GGCGGCGCCGGCTGGCCGGCCGCATCGCGCCCGCCGTGCAGACCTGGGGCTGCGCCTTCTCCGCGCCGACCGCCCGCATG
CAGTACACCGGCTCGTCGCTCGCCGCGCTGCTGGTGGCGCGCTTCTCCTGGGCGGTCCACCCGCGGCGCACCGAGCCGCG
GCTGGAGGGGCCGTTCCCGGCGCCGGCGCCGTTCGCCACCACGGTGCCGGACACCGTCCTCGACCTCGCCCTCCTCCCGG
CGGCGCGCGCGTACGGCTGGATCGCCGAGCGCGCGCGCCTCCTCTACCTCCGCAGGATCCACTTCCAGATGCTGCTCGTG
CTCGTGACGCTCCTCGCCGTCCTCGCCTGGGGGTTCGCGTGGTGA

Upstream 100 bases:

>100_bases
GGCTGCGCAGCTTCCAGCAGGCCCGCCTCAACCTCCAGCTCCTCTACACCGTCGCGGCGCTGCTCGGGCTCACGGCGCTG
CTGCTCCTCCGGTACGGTCC

Downstream 100 bases:

>100_bases
ACGCACCCATGGTCCTGCGCACCGCGCTCCACGTGGTCGCGCTGCTCGTGCTCCCGCCGCTGCTGCTCGGCGTGATCAAC
CGCGTGAAGTCCATCGTCGG

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 654; Mature: 653

Protein sequence:

>654_residues
MTSALALGAIALTAASGLAAALPGLRPARAQALATAVLCAGAALGLAAAGVALAGGEAAAGGVLRVDALSGLFLVPILGI
AAAGSAYGLRYFPQAERGARAVRLQLFYGLAVAGMALLVCAPDAIGFLVGWEVMALANFFMVQTEDEDPSVRRGAFLYLA
ATHAGTLALFALFALLRREAGTFELAAMEGLRLSGPTAAAVLALALAGFGMKAGLVPLHFWLPPAHAAAPSHVSALMSGV
VVKTGIYGLMRVTGLVESPPESWGVALLALGGVSAVLGVAFALAQHDLKRLLAYHTVENVGIIALGLGLALLGRARGVPA
LVVLGLGGAGLHVVNHALFKSLLFLGAGAVHHATGTRDLDHLGGLARAMPRTALLFVVGAAAISGLPPLNGFASEWLVYL
GLLDGLRQPGGDLLAYVTLGAPALALVGGLAAACFAKVVGVVFLGSPRSHHADGAHEPPAAMLAPMALLAAACAAIGLFP
AATTAPLARAAAAWARVDPALLAAPAARAAAGAARVSAVAAVLLVATLAVAAWRRRRLAGRIAPAVQTWGCAFSAPTARM
QYTGSSLAALLVARFSWAVHPRRTEPRLEGPFPAPAPFATTVPDTVLDLALLPAARAYGWIAERARLLYLRRIHFQMLLV
LVTLLAVLAWGFAW

Sequences:

>Translated_654_residues
MTSALALGAIALTAASGLAAALPGLRPARAQALATAVLCAGAALGLAAAGVALAGGEAAAGGVLRVDALSGLFLVPILGI
AAAGSAYGLRYFPQAERGARAVRLQLFYGLAVAGMALLVCAPDAIGFLVGWEVMALANFFMVQTEDEDPSVRRGAFLYLA
ATHAGTLALFALFALLRREAGTFELAAMEGLRLSGPTAAAVLALALAGFGMKAGLVPLHFWLPPAHAAAPSHVSALMSGV
VVKTGIYGLMRVTGLVESPPESWGVALLALGGVSAVLGVAFALAQHDLKRLLAYHTVENVGIIALGLGLALLGRARGVPA
LVVLGLGGAGLHVVNHALFKSLLFLGAGAVHHATGTRDLDHLGGLARAMPRTALLFVVGAAAISGLPPLNGFASEWLVYL
GLLDGLRQPGGDLLAYVTLGAPALALVGGLAAACFAKVVGVVFLGSPRSHHADGAHEPPAAMLAPMALLAAACAAIGLFP
AATTAPLARAAAAWARVDPALLAAPAARAAAGAARVSAVAAVLLVATLAVAAWRRRRLAGRIAPAVQTWGCAFSAPTARM
QYTGSSLAALLVARFSWAVHPRRTEPRLEGPFPAPAPFATTVPDTVLDLALLPAARAYGWIAERARLLYLRRIHFQMLLV
LVTLLAVLAWGFAW
>Mature_653_residues
TSALALGAIALTAASGLAAALPGLRPARAQALATAVLCAGAALGLAAAGVALAGGEAAAGGVLRVDALSGLFLVPILGIA
AAGSAYGLRYFPQAERGARAVRLQLFYGLAVAGMALLVCAPDAIGFLVGWEVMALANFFMVQTEDEDPSVRRGAFLYLAA
THAGTLALFALFALLRREAGTFELAAMEGLRLSGPTAAAVLALALAGFGMKAGLVPLHFWLPPAHAAAPSHVSALMSGVV
VKTGIYGLMRVTGLVESPPESWGVALLALGGVSAVLGVAFALAQHDLKRLLAYHTVENVGIIALGLGLALLGRARGVPAL
VVLGLGGAGLHVVNHALFKSLLFLGAGAVHHATGTRDLDHLGGLARAMPRTALLFVVGAAAISGLPPLNGFASEWLVYLG
LLDGLRQPGGDLLAYVTLGAPALALVGGLAAACFAKVVGVVFLGSPRSHHADGAHEPPAAMLAPMALLAAACAAIGLFPA
ATTAPLARAAAAWARVDPALLAAPAARAAAGAARVSAVAAVLLVATLAVAAWRRRRLAGRIAPAVQTWGCAFSAPTARMQ
YTGSSLAALLVARFSWAVHPRRTEPRLEGPFPAPAPFATTVPDTVLDLALLPAARAYGWIAERARLLYLRRIHFQMLLVL
VTLLAVLAWGFAW

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=436, Percent_Identity=24.5412844036697, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI251831116, Length=305, Percent_Identity=23.6065573770492, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788827, Length=448, Percent_Identity=43.0803571428571, Blast_Score=322, Evalue=6e-89,
Organism=Escherichia coli, GI2367154, Length=439, Percent_Identity=37.3576309794989, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1788831, Length=361, Percent_Identity=35.7340720221607, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1788829, Length=346, Percent_Identity=31.7919075144509, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788614, Length=353, Percent_Identity=31.4447592067989, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788613, Length=353, Percent_Identity=26.9121813031161, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 66470; Mature: 66339

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSALALGAIALTAASGLAAALPGLRPARAQALATAVLCAGAALGLAAAGVALAGGEAAA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
GGVLRVDALSGLFLVPILGIAAAGSAYGLRYFPQAERGARAVRLQLFYGLAVAGMALLVC
CCEEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHC
APDAIGFLVGWEVMALANFFMVQTEDEDPSVRRGAFLYLAATHAGTLALFALFALLRREA
CCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCC
GTFELAAMEGLRLSGPTAAAVLALALAGFGMKAGLVPLHFWLPPAHAAAPSHVSALMSGV
CCEEEEHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHCCCHHHHHHHHHHH
VVKTGIYGLMRVTGLVESPPESWGVALLALGGVSAVLGVAFALAQHDLKRLLAYHTVENV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIIALGLGLALLGRARGVPALVVLGLGGAGLHVVNHALFKSLLFLGAGAVHHATGTRDLD
CHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHH
HLGGLARAMPRTALLFVVGAAAISGLPPLNGFASEWLVYLGLLDGLRQPGGDLLAYVTLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
APALALVGGLAAACFAKVVGVVFLGSPRSHHADGAHEPPAAMLAPMALLAAACAAIGLFP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
AATTAPLARAAAAWARVDPALLAAPAARAAAGAARVSAVAAVLLVATLAVAAWRRRRLAG
CCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIAPAVQTWGCAFSAPTARMQYTGSSLAALLVARFSWAVHPRRTEPRLEGPFPAPAPFAT
HHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
TVPDTVLDLALLPAARAYGWIAERARLLYLRRIHFQMLLVLVTLLAVLAWGFAW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSALALGAIALTAASGLAAALPGLRPARAQALATAVLCAGAALGLAAAGVALAGGEAAA
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
GGVLRVDALSGLFLVPILGIAAAGSAYGLRYFPQAERGARAVRLQLFYGLAVAGMALLVC
CCEEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHC
APDAIGFLVGWEVMALANFFMVQTEDEDPSVRRGAFLYLAATHAGTLALFALFALLRREA
CCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCC
GTFELAAMEGLRLSGPTAAAVLALALAGFGMKAGLVPLHFWLPPAHAAAPSHVSALMSGV
CCEEEEHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHCCCHHHHHHHHHHH
VVKTGIYGLMRVTGLVESPPESWGVALLALGGVSAVLGVAFALAQHDLKRLLAYHTVENV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIIALGLGLALLGRARGVPALVVLGLGGAGLHVVNHALFKSLLFLGAGAVHHATGTRDLD
CHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHH
HLGGLARAMPRTALLFVVGAAAISGLPPLNGFASEWLVYLGLLDGLRQPGGDLLAYVTLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
APALALVGGLAAACFAKVVGVVFLGSPRSHHADGAHEPPAAMLAPMALLAAACAAIGLFP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
AATTAPLARAAAAWARVDPALLAAPAARAAAGAARVSAVAAVLLVATLAVAAWRRRRLAG
CCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIAPAVQTWGCAFSAPTARMQYTGSSLAALLVARFSWAVHPRRTEPRLEGPFPAPAPFAT
HHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
TVPDTVLDLALLPAARAYGWIAERARLLYLRRIHFQMLLVLVTLLAVLAWGFAW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]