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The map label for this gene is pdhC [H]

Identifier: 220917208

GI number: 220917208

Start: 2347176

End: 2348501

Strand: Direct

Name: pdhC [H]

Synonym: A2cp1_2105

Alternate gene names: 220917208

Gene position: 2347176-2348501 (Clockwise)

Preceding gene: 220917207

Following gene: 220917209

Centisome position: 46.67

GC content: 74.66

Gene sequence:

>1326_bases
ATGGCCTACAAGCTCGAGCTCCCCGACATCGGCGAGGGGGTCGTCGAGGCGGAGGTCCAGCAGTGGTTCGTCGCCCCGGG
CGACGCGATCACCGAGGATCAGCCGCTCGTCGAGGTGATGACCGACAAGGCGACGGTGGTGATCCCGTCGCCGAAGCGCG
GCACGGTGGTGAAGCTGTTCTGGAACGTGGGCGACGTCGCCAAGGTGCACTCGCCGCTGGTCGAGATCGAGCTCGACGAC
GCGCCGCCGCGCCCGGCGCCCGAGGCGCCGGTGCGCGCCGCCACCGCGGCCGCGACGGCCCAGGCGGCCGCGGCGGCGAG
CGTCGCCGTGGGCCGGCCGGCCCAGGCCGAGCCGCCCGCGGCGGCGGCGGCCCCCTCGGCGGCGGCAGGGCCCGCGGGCG
CGAAGGCGCTCGCCACCCCCGCCGTGCGCGCCATGGCGCGGGAGCTGGGGATCGACGTGAACACGGTGCCGGGCACCGGC
CCGGGCGGCCGCGTCACCAAGGACGACCTGTCGCGCGCGCGCGGCAAGGTGAACGGGCACGGCGGCGAGACGGAGGTGCC
GGCCCCGGCGCCCGCCGCGGCCCGCGCCCCTGTGCCGGCGCCGTCGCCGGCCGGCCCGCCGGAGGCGGACGAGCGGATCC
CGCTGCGCGGCATGCGGCGGAAGATCGCCGAGAACATGGCGCGCTCGAAGCGGACCGCCGCCCACTTCACGTTCGTGGAG
CAGGCGGACGTCACCGAGCTGGTCCGGGTGAAGGACCGGATCGCCGCCGCCGCGAAGGAGGAGGGCGTCCGCGTCACGTT
CCTGCCGTTCGTGGTGAAGGCGGTCGTCGCCGCCCTGCGCAAGTTCCCGAAGCTGAACGCGTCGCTCGACGACGAGCGCG
GCGAGATCGTGGTGAAGCACCGGTACGACCTCGGCGTCGCGTCGGCCACCGACGCGGGCCTGGTGGTCCCGGTGATCCGC
GCGGCCGATCGCCGCTCGCTCCTCGATCTCGCCCGGGAGATCGAGCGGCTCTCGCAGGACACCAAGGCCGGGCGCATCCG
CCCCGAGGACCTGGGCAGCTCCACCTTCACCATCACCAGCCTGGGCGCGCTGGGCGGGCTGTTCGCCACCCCGGTGCTGA
ACCACCCCGAGGTCGGCATCCTGGGCATCCACCGCATCCGGCCCACGCCGGTGGTGCGCGACGGGCAGATCGTCGCCCGC
GACGTGATGCACGTCTCGCTCACGAGCGACCACCGCGTGGTGGACGGGCACGAGGCGGCCGCGTTCACCTACCAGGTGAT
CAGGTACCTCGAGGATCCCAACCTGCTGTTCATGCAGATGGTGTGA

Upstream 100 bases:

>100_bases
ACGCCGTTCCCGTACACGCTCGAGAACGAGTACCTGCCGCGCGCGCCGCGCATCCTGAAGGCCATCCGCGAGGTGGTCGC
GTACTGAGGGGGAGTCGTCC

Downstream 100 bases:

>100_bases
TCGACTCGCACTGCCACCTCGACGCGCGCGCGTTCGACGCGGACCGGGCCGAGGCGCTGGCCCGGGCGCGCGCCGCGGGG
GTCACCGACGTGATCGTGCC

Product: hypothetical protein

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAYKLELPDIGEGVVEAEVQQWFVAPGDAITEDQPLVEVMTDKATVVIPSPKRGTVVKLFWNVGDVAKVHSPLVEIELDD
APPRPAPEAPVRAATAAATAQAAAAASVAVGRPAQAEPPAAAAAPSAAAGPAGAKALATPAVRAMARELGIDVNTVPGTG
PGGRVTKDDLSRARGKVNGHGGETEVPAPAPAAARAPVPAPSPAGPPEADERIPLRGMRRKIAENMARSKRTAAHFTFVE
QADVTELVRVKDRIAAAAKEEGVRVTFLPFVVKAVVAALRKFPKLNASLDDERGEIVVKHRYDLGVASATDAGLVVPVIR
AADRRSLLDLAREIERLSQDTKAGRIRPEDLGSSTFTITSLGALGGLFATPVLNHPEVGILGIHRIRPTPVVRDGQIVAR
DVMHVSLTSDHRVVDGHEAAAFTYQVIRYLEDPNLLFMQMV

Sequences:

>Translated_441_residues
MAYKLELPDIGEGVVEAEVQQWFVAPGDAITEDQPLVEVMTDKATVVIPSPKRGTVVKLFWNVGDVAKVHSPLVEIELDD
APPRPAPEAPVRAATAAATAQAAAAASVAVGRPAQAEPPAAAAAPSAAAGPAGAKALATPAVRAMARELGIDVNTVPGTG
PGGRVTKDDLSRARGKVNGHGGETEVPAPAPAAARAPVPAPSPAGPPEADERIPLRGMRRKIAENMARSKRTAAHFTFVE
QADVTELVRVKDRIAAAAKEEGVRVTFLPFVVKAVVAALRKFPKLNASLDDERGEIVVKHRYDLGVASATDAGLVVPVIR
AADRRSLLDLAREIERLSQDTKAGRIRPEDLGSSTFTITSLGALGGLFATPVLNHPEVGILGIHRIRPTPVVRDGQIVAR
DVMHVSLTSDHRVVDGHEAAAFTYQVIRYLEDPNLLFMQMV
>Mature_440_residues
AYKLELPDIGEGVVEAEVQQWFVAPGDAITEDQPLVEVMTDKATVVIPSPKRGTVVKLFWNVGDVAKVHSPLVEIELDDA
PPRPAPEAPVRAATAAATAQAAAAASVAVGRPAQAEPPAAAAAPSAAAGPAGAKALATPAVRAMARELGIDVNTVPGTGP
GGRVTKDDLSRARGKVNGHGGETEVPAPAPAAARAPVPAPSPAGPPEADERIPLRGMRRKIAENMARSKRTAAHFTFVEQ
ADVTELVRVKDRIAAAAKEEGVRVTFLPFVVKAVVAALRKFPKLNASLDDERGEIVVKHRYDLGVASATDAGLVVPVIRA
ADRRSLLDLAREIERLSQDTKAGRIRPEDLGSSTFTITSLGALGGLFATPVLNHPEVGILGIHRIRPTPVVRDGQIVARD
VMHVSLTSDHRVVDGHEAAAFTYQVIRYLEDPNLLFMQMV

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=449, Percent_Identity=30.2895322939866, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI19923748, Length=245, Percent_Identity=33.8775510204082, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI31711992, Length=448, Percent_Identity=28.5714285714286, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI203098816, Length=467, Percent_Identity=24.6252676659529, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI203098753, Length=467, Percent_Identity=24.6252676659529, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI260898739, Length=165, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786946, Length=436, Percent_Identity=30.045871559633, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1786305, Length=445, Percent_Identity=32.1348314606742, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=440, Percent_Identity=31.5909090909091, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI25146366, Length=445, Percent_Identity=30.1123595505618, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=454, Percent_Identity=27.9735682819383, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=330, Percent_Identity=28.1818181818182, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=442, Percent_Identity=29.8642533936652, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6324258, Length=443, Percent_Identity=22.7990970654628, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI18859875, Length=441, Percent_Identity=31.7460317460317, Blast_Score=224, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=32.7510917030568, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24582497, Length=234, Percent_Identity=28.6324786324786, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI20129315, Length=234, Percent_Identity=28.6324786324786, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46476; Mature: 46344

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYKLELPDIGEGVVEAEVQQWFVAPGDAITEDQPLVEVMTDKATVVIPSPKRGTVVKLF
CEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCEEEEECCCCCCCEEEEE
WNVGDVAKVHSPLVEIELDDAPPRPAPEAPVRAATAAATAQAAAAASVAVGRPAQAEPPA
ECCCCHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AAAAPSAAAGPAGAKALATPAVRAMARELGIDVNTVPGTGPGGRVTKDDLSRARGKVNGH
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHCCCCCCC
GGETEVPAPAPAAARAPVPAPSPAGPPEADERIPLRGMRRKIAENMARSKRTAAHFTFVE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEC
QADVTELVRVKDRIAAAAKEEGVRVTFLPFVVKAVVAALRKFPKLNASLDDERGEIVVKH
CCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
RYDLGVASATDAGLVVPVIRAADRRSLLDLAREIERLSQDTKAGRIRPEDLGSSTFTITS
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEH
LGALGGLFATPVLNHPEVGILGIHRIRPTPVVRDGQIVARDVMHVSLTSDHRVVDGHEAA
HHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEECCCEEEEHEEEEEECCCCEEECCCHHH
AFTYQVIRYLEDPNLLFMQMV
HHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure 
AYKLELPDIGEGVVEAEVQQWFVAPGDAITEDQPLVEVMTDKATVVIPSPKRGTVVKLF
EEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCEEEEECCCCCCCEEEEE
WNVGDVAKVHSPLVEIELDDAPPRPAPEAPVRAATAAATAQAAAAASVAVGRPAQAEPPA
ECCCCHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AAAAPSAAAGPAGAKALATPAVRAMARELGIDVNTVPGTGPGGRVTKDDLSRARGKVNGH
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHCCCCCCC
GGETEVPAPAPAAARAPVPAPSPAGPPEADERIPLRGMRRKIAENMARSKRTAAHFTFVE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEC
QADVTELVRVKDRIAAAAKEEGVRVTFLPFVVKAVVAALRKFPKLNASLDDERGEIVVKH
CCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
RYDLGVASATDAGLVVPVIRAADRRSLLDLAREIERLSQDTKAGRIRPEDLGSSTFTITS
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEH
LGALGGLFATPVLNHPEVGILGIHRIRPTPVVRDGQIVARDVMHVSLTSDHRVVDGHEAA
HHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEECCCEEEEHEEEEEECCCCEEECCCHHH
AFTYQVIRYLEDPNLLFMQMV
HHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]