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The map label for this gene is bkdA2 [H]

Identifier: 220917207

GI number: 220917207

Start: 2346188

End: 2347162

Strand: Direct

Name: bkdA2 [H]

Synonym: A2cp1_2104

Alternate gene names: 220917207

Gene position: 2346188-2347162 (Clockwise)

Preceding gene: 220917206

Following gene: 220917208

Centisome position: 46.65

GC content: 69.85

Gene sequence:

>975_bases
ATGCCCACCATGAACATCATCCAGGCGGTCAACGACGCGCTCCGGATCGAGATGCGCAAGGATCCGGACGTGGTGGTGCT
GGGCGAGGACGTCGGGAAGTTCGGCGGCGTGTTCCGCGCCACGCAGGGGCTCTACGACGAGTTCGGCGCCGACCGTGTCA
TCGACACCCCGCTCGCCGAGGGCGGCATCATCGGCACCGCGGTGGGCATGGCGCTCTACGGCCTGAAGCCGGTGCCGGAG
ATCCAGTTCGCCGACTTCATCTTCCCGGCGTTCGACCAGATCGTGAACGAGGTGGCGAAGTACCGCTACCGCTCCGGCGG
CCAGTACGCCTGCCCCATGGTCATCCGCACGCCGTACGGCGGCGGCATCAAGGGCGGCCACTACCACTCGCAGTCGCCCG
AGGCGATGTTCATCCACACCGCCGGCCTGAAGGTGGTCGTCCCGTCCAACCCCTACGACGCCAAGGGGCTCCTCATCAGC
GCCATCCGCGACCCGGACCCGGTGCTGTTCTTCGAGCCGAAGCGCGTCTACCGCGCCGCCAAGGGCGACGTGCCCGAAGG
CGAGTACGCCGAGCCGCTCGGCAAGGCCAGGATCACGCGCGCCGGCAACCAGGTGACGGTGATGGCCTGGGGCTCGATGT
GGCACGAGGTGGACCAGGCCGCGCGCGAGGCGGCGGCCGAGGGGTTCGACTGCGAGGTCATCGACCTCCGCTCGCTCCAG
CCGCTCGACCTCGAGACCATCGTGGCGTCGGTGTCGAAGACCGGGCGCGCCATCGTGGTGCACGAGGCGCCGCGCACCTG
CGGGTTCGGCGCGGAGATCGCCGCGCTGGTCCAGGAGCGCTGCTTCCTGCACCTCGAGGCGCCGGTGGCGCGCGTCACCG
GCTTCGACACGCCGTTCCCGTACACGCTCGAGAACGAGTACCTGCCGCGCGCGCCGCGCATCCTGAAGGCCATCCGCGAG
GTGGTCGCGTACTGA

Upstream 100 bases:

>100_bases
TGGCAGCAGCGCGAGCAGCTGGCCGAGATCGAGGCGGCGGTCGCCGCGGACCCGCGCGCGGCGAACCCGCGCCACTCCGA
CGCGTGATACAGGGGGCCGC

Downstream 100 bases:

>100_bases
GGGGGAGTCGTCCATGGCCTACAAGCTCGAGCTCCCCGACATCGGCGAGGGGGTCGTCGAGGCGGAGGTCCAGCAGTGGT
TCGTCGCCCCGGGCGACGCG

Product: transketolase central subunit

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MPTMNIIQAVNDALRIEMRKDPDVVVLGEDVGKFGGVFRATQGLYDEFGADRVIDTPLAEGGIIGTAVGMALYGLKPVPE
IQFADFIFPAFDQIVNEVAKYRYRSGGQYACPMVIRTPYGGGIKGGHYHSQSPEAMFIHTAGLKVVVPSNPYDAKGLLIS
AIRDPDPVLFFEPKRVYRAAKGDVPEGEYAEPLGKARITRAGNQVTVMAWGSMWHEVDQAAREAAAEGFDCEVIDLRSLQ
PLDLETIVASVSKTGRAIVVHEAPRTCGFGAEIAALVQERCFLHLEAPVARVTGFDTPFPYTLENEYLPRAPRILKAIRE
VVAY

Sequences:

>Translated_324_residues
MPTMNIIQAVNDALRIEMRKDPDVVVLGEDVGKFGGVFRATQGLYDEFGADRVIDTPLAEGGIIGTAVGMALYGLKPVPE
IQFADFIFPAFDQIVNEVAKYRYRSGGQYACPMVIRTPYGGGIKGGHYHSQSPEAMFIHTAGLKVVVPSNPYDAKGLLIS
AIRDPDPVLFFEPKRVYRAAKGDVPEGEYAEPLGKARITRAGNQVTVMAWGSMWHEVDQAAREAAAEGFDCEVIDLRSLQ
PLDLETIVASVSKTGRAIVVHEAPRTCGFGAEIAALVQERCFLHLEAPVARVTGFDTPFPYTLENEYLPRAPRILKAIRE
VVAY
>Mature_323_residues
PTMNIIQAVNDALRIEMRKDPDVVVLGEDVGKFGGVFRATQGLYDEFGADRVIDTPLAEGGIIGTAVGMALYGLKPVPEI
QFADFIFPAFDQIVNEVAKYRYRSGGQYACPMVIRTPYGGGIKGGHYHSQSPEAMFIHTAGLKVVVPSNPYDAKGLLISA
IRDPDPVLFFEPKRVYRAAKGDVPEGEYAEPLGKARITRAGNQVTVMAWGSMWHEVDQAAREAAAEGFDCEVIDLRSLQP
LDLETIVASVSKTGRAIVVHEAPRTCGFGAEIAALVQERCFLHLEAPVARVTGFDTPFPYTLENEYLPRAPRILKAIREV
VAY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=323, Percent_Identity=52.3219814241486, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI34101272, Length=323, Percent_Identity=52.3219814241486, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI156564403, Length=320, Percent_Identity=39.6875, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI291084858, Length=320, Percent_Identity=37.8125, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17506935, Length=323, Percent_Identity=54.4891640866873, Blast_Score=341, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17538422, Length=324, Percent_Identity=41.358024691358, Blast_Score=234, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6319698, Length=328, Percent_Identity=40.5487804878049, Blast_Score=233, Evalue=4e-62,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=52.3219814241486, Blast_Score=341, Evalue=4e-94,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=52.3219814241486, Blast_Score=341, Evalue=5e-94,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=41.2307692307692, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=41.2307692307692, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=41.1111111111111, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=41.1111111111111, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 35430; Mature: 35299

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTMNIIQAVNDALRIEMRKDPDVVVLGEDVGKFGGVFRATQGLYDEFGADRVIDTPLAE
CCCHHHHHHHHHHHEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHCCCCEECCCCCC
GGIIGTAVGMALYGLKPVPEIQFADFIFPAFDQIVNEVAKYRYRSGGQYACPMVIRTPYG
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEECCCC
GGIKGGHYHSQSPEAMFIHTAGLKVVVPSNPYDAKGLLISAIRDPDPVLFFEPKRVYRAA
CCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCCEEEEEECCCCCEEEECHHHHHHHH
KGDVPEGEYAEPLGKARITRAGNQVTVMAWGSMWHEVDQAAREAAAEGFDCEVIDLRSLQ
CCCCCCCCHHCHHHHHHHEECCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
PLDLETIVASVSKTGRAIVVHEAPRTCGFGAEIAALVQERCFLHLEAPVARVTGFDTPFP
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHEEEECCCHHHHCCCCCCCC
YTLENEYLPRAPRILKAIREVVAY
CCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PTMNIIQAVNDALRIEMRKDPDVVVLGEDVGKFGGVFRATQGLYDEFGADRVIDTPLAE
CCHHHHHHHHHHHEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHCCCCEECCCCCC
GGIIGTAVGMALYGLKPVPEIQFADFIFPAFDQIVNEVAKYRYRSGGQYACPMVIRTPYG
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEECCCC
GGIKGGHYHSQSPEAMFIHTAGLKVVVPSNPYDAKGLLISAIRDPDPVLFFEPKRVYRAA
CCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCCEEEEEECCCCCEEEECHHHHHHHH
KGDVPEGEYAEPLGKARITRAGNQVTVMAWGSMWHEVDQAAREAAAEGFDCEVIDLRSLQ
CCCCCCCCHHCHHHHHHHEECCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
PLDLETIVASVSKTGRAIVVHEAPRTCGFGAEIAALVQERCFLHLEAPVARVTGFDTPFP
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHEEEECCCHHHHCCCCCCCC
YTLENEYLPRAPRILKAIREVVAY
CCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3416875; 10426958 [H]